EA023764B9 - Генетически модифицированная клетка и способ использования упомянутой клетки - Google Patents

Генетически модифицированная клетка и способ использования упомянутой клетки

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Publication number
EA023764B9
EA023764B9 EA201300999A EA201300999A EA023764B9 EA 023764 B9 EA023764 B9 EA 023764B9 EA 201300999 A EA201300999 A EA 201300999A EA 201300999 A EA201300999 A EA 201300999A EA 023764 B9 EA023764 B9 EA 023764B9
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EA
Eurasian Patent Office
Prior art keywords
cell
genetically modified
biotransformation
relates
present
Prior art date
Application number
EA201300999A
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English (en)
Other versions
EA201300999A1 (ru
EA023764B1 (ru
Inventor
Ник Йоханнес Петрус Виркс
Том Даниель Элинк Схюрман
Сипко Мартен Кюйпер
Харалд Йохан Рёйссенарс
Original Assignee
Пюрак Биохем Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пюрак Биохем Б.В. filed Critical Пюрак Биохем Б.В.
Publication of EA201300999A1 publication Critical patent/EA201300999A1/ru
Publication of EA023764B1 publication Critical patent/EA023764B1/ru
Publication of EA023764B9 publication Critical patent/EA023764B9/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к области биотрансформации фурановых соединений. Более конкретно, изобретение относится к новым генетически модифицированным клеткам с улучшенными характеристиками для биокаталитической трансформации фурановых соединений и к вектору, пригодному для генетической модификации клетки-хозяина. Другой объект изобретения нацелен на способы биотрансформации 5-(гидроксиметил)фуран-2-карбоновой кислоты (ГМФ-кислоты) и ее предшественников с использованием клеток изобретения.
EA201300999A 2011-03-08 2012-03-07 Генетически модифицированная клетка и способ использования упомянутой клетки EA023764B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2006359A NL2006359C2 (en) 2011-03-08 2011-03-08 Genetically modified cell and process for use of said cell.
PCT/NL2012/050141 WO2012064195A2 (en) 2011-03-08 2012-03-07 Genetically modified cell and process for use of said cell

Publications (3)

Publication Number Publication Date
EA201300999A1 EA201300999A1 (ru) 2014-02-28
EA023764B1 EA023764B1 (ru) 2016-07-29
EA023764B9 true EA023764B9 (ru) 2016-10-31

Family

ID=45873204

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201300999A EA023764B9 (ru) 2011-03-08 2012-03-07 Генетически модифицированная клетка и способ использования упомянутой клетки

Country Status (19)

Country Link
US (3) US9309546B2 (ru)
EP (1) EP2683815B1 (ru)
JP (2) JP6017463B2 (ru)
KR (1) KR101947202B1 (ru)
CN (1) CN103502441B (ru)
AU (1) AU2012202889B2 (ru)
BR (1) BR112013022915B1 (ru)
CA (1) CA2829078C (ru)
DK (1) DK2683815T3 (ru)
EA (1) EA023764B9 (ru)
ES (1) ES2543568T3 (ru)
HU (1) HUE027066T2 (ru)
MX (1) MX336694B (ru)
MY (1) MY161455A (ru)
NL (1) NL2006359C2 (ru)
PL (1) PL2683815T3 (ru)
PT (1) PT2683815E (ru)
SI (1) SI2683815T1 (ru)
WO (1) WO2012064195A2 (ru)

Families Citing this family (23)

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NL2006359C2 (en) * 2011-03-08 2012-04-24 Bird Engineering B V Genetically modified cell and process for use of said cell.
US20150152452A1 (en) * 2012-07-20 2015-06-04 Novozymes A/S Enzymatic Oxidation of 5-Hydroxymethylfurfural and Derivatives Thereof
US9528133B2 (en) 2012-09-21 2016-12-27 Synthetic Genomics, Inc. Compositions and methods for producing chemicals and derivatives thereof
CN104769122B (zh) 2012-09-21 2018-08-10 合成基因组股份有限公司 用于生产化学品和其衍生物的组合物和方法
WO2014081973A1 (en) 2012-11-21 2014-05-30 The Regents Of The University Of California Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic archaea
NL2011027C2 (en) 2013-06-24 2014-12-29 Univ Delft Tech Process for the preparation of 2,5-furandicarboxylic acid.
CN104371955B (zh) * 2014-10-30 2017-02-08 江南大学 一种合成2,5‑呋喃二甲酸的土生拉乌尔菌及其应用
EP3259349B1 (en) * 2015-02-17 2020-06-17 Purac Biochem B.V. Dehydrogenase-catalysed production of fdca
WO2017050815A1 (en) 2015-09-21 2017-03-30 Purac Biochem B.V. Fungal production of fdca
JP7071283B2 (ja) * 2016-12-20 2022-05-18 株式会社カネカ ポリヒドロキシアルカン酸の製造方法及び微生物
EP3366681A1 (en) 2017-02-28 2018-08-29 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Production of 2,5-furandicarboxylic acid
EP3378931A1 (en) * 2017-03-21 2018-09-26 Purac Biochem B.V. Fdca-decarboxylating monooxygenase-deficient host cells for producing fdca
CN109609397A (zh) * 2017-10-31 2019-04-12 天津科技大学 一种水产养殖用复合微生物菌剂及应用
WO2019094740A1 (en) 2017-11-10 2019-05-16 Shell Oil Company Methods of producing 2,5-furandicarboxylic acid
EP3732174B1 (en) 2017-12-29 2023-12-27 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO Method for purifying and removing colour of 2,5-furandicarboxylic acid (fdca)
EP3505514A1 (en) 2017-12-29 2019-07-03 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Method for purifying and removing color of 2,5-furandicarboxylic acid (fdca)
US11339414B2 (en) * 2019-03-01 2022-05-24 Braskem S.A. Method for the in vivo synthesis of 4-hydroxymethylfurfural and derivatives thereof
CN110591995A (zh) * 2019-09-12 2019-12-20 华南理工大学 一种共表达重组菌及其在合成呋喃羧酸中的应用
CN110724654B (zh) * 2019-11-22 2020-09-29 南京科技职业学院 一株生产5-羟甲基-2呋喃甲酸的铜绿假单胞菌及其应用
TWI735113B (zh) * 2019-12-23 2021-08-01 國立清華大學 ２,５-呋喃二甲酸之製備方法
NL2024726B1 (en) 2020-01-22 2021-09-09 Biomosae B V Enzymatic crop protection and process for preparing biological crop protection composition
US20240011990A1 (en) 2020-07-17 2024-01-11 Trustees Of Boston University Viral detection systems and uses thereof
US20240115607A1 (en) 2021-01-26 2024-04-11 Cytocares (Shanghai) Inc. Chimeric antigen receptor (car) constructs and nk cells expressing car constructs

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Also Published As

Publication number Publication date
BR112013022915A2 (pt) 2016-12-06
EP2683815B1 (en) 2015-05-20
HUE027066T2 (en) 2016-08-29
EP2683815A2 (en) 2014-01-15
US10023890B2 (en) 2018-07-17
ES2543568T3 (es) 2015-08-20
WO2012064195A2 (en) 2012-05-18
JP2017060471A (ja) 2017-03-30
AU2012202889B2 (en) 2016-09-15
PL2683815T3 (pl) 2015-10-30
WO2012064195A3 (en) 2012-08-23
BR112013022915B1 (pt) 2021-08-31
KR101947202B1 (ko) 2019-02-12
DK2683815T3 (en) 2015-07-20
US20180023105A1 (en) 2018-01-25
MX336694B (es) 2016-01-27
MX2013010226A (es) 2014-01-08
US9309546B2 (en) 2016-04-12
NL2006359C2 (en) 2012-04-24
SI2683815T1 (sl) 2015-09-30
US9828616B2 (en) 2017-11-28
AU2012202889A1 (en) 2013-09-26
CN103502441B (zh) 2016-09-14
US20160257978A1 (en) 2016-09-08
KR20140015439A (ko) 2014-02-06
EA201300999A1 (ru) 2014-02-28
US20140073019A1 (en) 2014-03-13
CA2829078C (en) 2021-12-28
MY161455A (en) 2017-04-14
CA2829078A1 (en) 2012-05-18
JP6327724B2 (ja) 2018-05-23
PT2683815E (pt) 2015-09-18
JP2014508531A (ja) 2014-04-10
JP6017463B2 (ja) 2016-11-02
EA023764B1 (ru) 2016-07-29
CN103502441A (zh) 2014-01-08

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