EA023764B9 - Генетически модифицированная клетка и способ использования упомянутой клетки - Google Patents

Генетически модифицированная клетка и способ использования упомянутой клетки

Info

Publication number
EA023764B9
EA023764B9 EA201300999A EA201300999A EA023764B9 EA 023764 B9 EA023764 B9 EA 023764B9 EA 201300999 A EA201300999 A EA 201300999A EA 201300999 A EA201300999 A EA 201300999A EA 023764 B9 EA023764 B9 EA 023764B9
Authority
EA
Eurasian Patent Office
Prior art keywords
cell
genetically modified
biotransformation
relates
present
Prior art date
Application number
EA201300999A
Other languages
English (en)
Other versions
EA201300999A1 (ru
EA023764B1 (ru
Inventor
Ник Йоханнес Петрус Виркс
Том Даниель Элинк Схюрман
Сипко Мартен Кюйпер
Харалд Йохан Рёйссенарс
Original Assignee
Пюрак Биохем Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пюрак Биохем Б.В. filed Critical Пюрак Биохем Б.В.
Publication of EA201300999A1 publication Critical patent/EA201300999A1/ru
Publication of EA023764B1 publication Critical patent/EA023764B1/ru
Publication of EA023764B9 publication Critical patent/EA023764B9/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к области биотрансформации фурановых соединений. Более конкретно, изобретение относится к новым генетически модифицированным клеткам с улучшенными характеристиками для биокаталитической трансформации фурановых соединений и к вектору, пригодному для генетической модификации клетки-хозяина. Другой объект изобретения нацелен на способы биотрансформации 5-(гидроксиметил)фуран-2-карбоновой кислоты (ГМФ-кислоты) и ее предшественников с использованием клеток изобретения.
EA201300999A 2011-03-08 2012-03-07 Генетически модифицированная клетка и способ использования упомянутой клетки EA023764B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2006359A NL2006359C2 (en) 2011-03-08 2011-03-08 Genetically modified cell and process for use of said cell.
PCT/NL2012/050141 WO2012064195A2 (en) 2011-03-08 2012-03-07 Genetically modified cell and process for use of said cell

Publications (3)

Publication Number Publication Date
EA201300999A1 EA201300999A1 (ru) 2014-02-28
EA023764B1 EA023764B1 (ru) 2016-07-29
EA023764B9 true EA023764B9 (ru) 2016-10-31

Family

ID=45873204

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201300999A EA023764B9 (ru) 2011-03-08 2012-03-07 Генетически модифицированная клетка и способ использования упомянутой клетки

Country Status (19)

Country Link
US (3) US9309546B2 (ru)
EP (1) EP2683815B1 (ru)
JP (2) JP6017463B2 (ru)
KR (1) KR101947202B1 (ru)
CN (1) CN103502441B (ru)
AU (1) AU2012202889B2 (ru)
BR (1) BR112013022915B1 (ru)
CA (1) CA2829078C (ru)
DK (1) DK2683815T3 (ru)
EA (1) EA023764B9 (ru)
ES (1) ES2543568T3 (ru)
HU (1) HUE027066T2 (ru)
MX (1) MX336694B (ru)
MY (1) MY161455A (ru)
NL (1) NL2006359C2 (ru)
PL (1) PL2683815T3 (ru)
PT (1) PT2683815E (ru)
SI (1) SI2683815T1 (ru)
WO (1) WO2012064195A2 (ru)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2006359C2 (en) * 2011-03-08 2012-04-24 Bird Engineering B V Genetically modified cell and process for use of said cell.
WO2014015256A2 (en) * 2012-07-20 2014-01-23 Novozymes A/S Enzymatic oxidation of 5-hydroxymethylfurfural and derivatives thereof
JP6163557B2 (ja) 2012-09-21 2017-07-12 シンセティック ジェノミクス インコーポレーテッド 化学物質およびその誘導体を生成するための組成物および方法
US9528133B2 (en) 2012-09-21 2016-12-27 Synthetic Genomics, Inc. Compositions and methods for producing chemicals and derivatives thereof
WO2014081973A1 (en) * 2012-11-21 2014-05-30 The Regents Of The University Of California Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic archaea
NL2011027C2 (en) 2013-06-24 2014-12-29 Univ Delft Tech Process for the preparation of 2,5-furandicarboxylic acid.
CN104371955B (zh) * 2014-10-30 2017-02-08 江南大学 一种合成2,5‑呋喃二甲酸的土生拉乌尔菌及其应用
AU2016220612B2 (en) * 2015-02-17 2021-09-30 Purac Biochem B.V. Dehydrogenase-catalysed production of FDCA
WO2017050815A1 (en) 2015-09-21 2017-03-30 Purac Biochem B.V. Fungal production of fdca
US10876140B2 (en) * 2016-12-20 2020-12-29 Kaneka Corporation Method for producing polyhydroxyalkanoic acid, and microbes
EP3366681A1 (en) 2017-02-28 2018-08-29 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Production of 2,5-furandicarboxylic acid
EP3378931A1 (en) 2017-03-21 2018-09-26 Purac Biochem B.V. Fdca-decarboxylating monooxygenase-deficient host cells for producing fdca
CN109609397A (zh) * 2017-10-31 2019-04-12 天津科技大学 一种水产养殖用复合微生物菌剂及应用
US20210180095A1 (en) 2017-11-10 2021-06-17 Shell Oil Company Methods of producing 2,5-furandicarboxylic acid
EP3505514A1 (en) 2017-12-29 2019-07-03 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Method for purifying and removing color of 2,5-furandicarboxylic acid (fdca)
EP3732174B1 (en) 2017-12-29 2023-12-27 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO Method for purifying and removing colour of 2,5-furandicarboxylic acid (fdca)
EP3921435A1 (en) * 2019-03-01 2021-12-15 Braskem S.A. Method for the in vivo synthesis of 4-hydroxymethylfurfural and derivatives thereof
CN110591995A (zh) * 2019-09-12 2019-12-20 华南理工大学 一种共表达重组菌及其在合成呋喃羧酸中的应用
CN110724654B (zh) * 2019-11-22 2020-09-29 南京科技职业学院 一株生产5-羟甲基-2呋喃甲酸的铜绿假单胞菌及其应用
TWI735113B (zh) 2019-12-23 2021-08-01 國立清華大學 ２,５-呋喃二甲酸之製備方法
NL2024726B1 (en) 2020-01-22 2021-09-09 Biomosae B V Enzymatic crop protection and process for preparing biological crop protection composition
WO2022016071A1 (en) 2020-07-17 2022-01-20 Trustees Of Boston University Viral detection systems and uses thereof
US20240115607A1 (en) 2021-01-26 2024-04-11 Cytocares (Shanghai) Inc. Chimeric antigen receptor (car) constructs and nk cells expressing car constructs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011026906A2 (en) * 2009-09-02 2011-03-10 Shell Internationale Research Maatschappij B.V. Novel microorganism and its use in lignocellulose detoxification

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2777503C (en) * 2009-09-02 2018-10-16 Dsm Ip Assets B.V. Polypeptides having oxidoreductase activity and their uses
NL2006359C2 (en) * 2011-03-08 2012-04-24 Bird Engineering B V Genetically modified cell and process for use of said cell.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011026906A2 (en) * 2009-09-02 2011-03-10 Shell Internationale Research Maatschappij B.V. Novel microorganism and its use in lignocellulose detoxification

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt 1 March 2008 (2008-03-01), COPELAND A, ET AL: "SubName: Full=Major facilitator superfamily MFS_1;", XP002660638, Database accession no. B1M730 *
DATABASE UniProt 2 August 2005 (2005-08-02), CHEN L, ET AL: "SubName: Full=Conserved transport protein;", XP002660639, Database accession no. Unreviewed *
F. KOOPMAN, WIERCKX N., DE WINDE J. H., RUIJSSENAARS H. J.: "Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14, supplemental information", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 11, 16 March 2010 (2010-03-16), pages 1 - 4, XP055008778, ISSN: 00278424, DOI: 10.1073/pnas.0913039107 *
FRANK KOOPMAN, NICK WIERCKX, JOHANNES H. DE WINDE, HARALD J. RUIJSSENAARS: "Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid", BIORESOURCE TECHNOLOGY, ELSEVIER, vol. 101, no. 16, 1 August 2010 (2010-08-01), pages 6291 - 6296, XP055008790, ISSN: 09608524, DOI: 10.1016/j.biortech.2010.03.050 *
KOOPMAN FRANK ET AL: "Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 107, no. 11, 16 March 2010 (2010-03-16), US, pages 4919 - 4924, XP007913659, ISSN: 0027-8424, DOI: 10.1073/pnas.0913039107 *
MARCELO L. RIBEIRO, ULF SCHUCHARDT: "Cooperative effect of cobalt acetylacetonate and silica in the catalytic cyclization and oxidation of fructose to 2,5-furandicarboxylic acid", CATALYSIS COMMUNICATIONS, ELSEVIER, vol. 4, no. 2, 1 February 2003 (2003-02-01), pages 83 - 86, XP055008814, ISSN: 15667367, DOI: 10.1016/S1566-7367(02)00261-3 *

Also Published As

Publication number Publication date
HUE027066T2 (en) 2016-08-29
AU2012202889B2 (en) 2016-09-15
EP2683815B1 (en) 2015-05-20
CN103502441B (zh) 2016-09-14
US20180023105A1 (en) 2018-01-25
EA201300999A1 (ru) 2014-02-28
CN103502441A (zh) 2014-01-08
CA2829078C (en) 2021-12-28
BR112013022915B1 (pt) 2021-08-31
PL2683815T3 (pl) 2015-10-30
JP6327724B2 (ja) 2018-05-23
US9828616B2 (en) 2017-11-28
CA2829078A1 (en) 2012-05-18
MY161455A (en) 2017-04-14
ES2543568T3 (es) 2015-08-20
SI2683815T1 (sl) 2015-09-30
PT2683815E (pt) 2015-09-18
US20140073019A1 (en) 2014-03-13
NL2006359C2 (en) 2012-04-24
JP6017463B2 (ja) 2016-11-02
JP2017060471A (ja) 2017-03-30
EP2683815A2 (en) 2014-01-15
DK2683815T3 (en) 2015-07-20
KR101947202B1 (ko) 2019-02-12
KR20140015439A (ko) 2014-02-06
WO2012064195A3 (en) 2012-08-23
EA023764B1 (ru) 2016-07-29
US20160257978A1 (en) 2016-09-08
MX2013010226A (es) 2014-01-08
JP2014508531A (ja) 2014-04-10
US9309546B2 (en) 2016-04-12
US10023890B2 (en) 2018-07-17
WO2012064195A2 (en) 2012-05-18
AU2012202889A1 (en) 2013-09-26
MX336694B (es) 2016-01-27
BR112013022915A2 (pt) 2016-12-06

Similar Documents

Publication Publication Date Title
MY161455A (en) Genetically modified cell and process for use of said cell
MX2019004568A (es) Produccion mejorada de derivados de acidos grasos.
WO2013076144A3 (en) Microorganism strains for the production of 2,3-butanediol
GEP20176628B (en) Targeted genomic alteration
MX2020009460A (es) Disminuir nivel de lactato e incrementar produccion de polipeptidos por regulacion a la baja de la expresion de lactato dehidrogenasa y piruvato dehidrogenasa quinasa.
WO2012173659A3 (en) Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
WO2013049121A3 (en) Recombinant phage and methods
WO2014022702A3 (en) Methods and compositions for controlling gene expression by rna processing
MX2015005251A (es) Metodos para producir 5-(halometil)furfural.
SG10201810830UA (en) Cell culture compositions and methods for polypeptide production
NZ627373A (en) Genes involved in noscapine production
MX2013007650A (es) Metodo de cultivo de celulas animales.
WO2012103261A3 (en) Compositions and methods for succinate production
NZ719881A (en) Plant cytochrome p450
MX2019007766A (es) Variantes de enzima con propiedades de ester sintasa mejoradas.
WO2012142094A3 (en) Compositions and methods for increased ethanol production from biomass
EP2831234A4 (en) COMPOSITIONS, METHODS AND GENES OF PLANTS FOR BETTER PRODUCTION OF FERMENTABLE SUGARS FOR BIOFUELS PRODUCTION
EP2647698A4 (en) PROCESS FOR PRODUCING EOSINOPHILES FROM PLURIPOTENT STEM CELLS
WO2012054935A3 (en) Formation of hematopoietic progenitor cells from mesenchymal stem cells
MX2013009403A (es) Agrupamiento genico para la biosintesis de cicloclavina.
CA2865497A1 (en) Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
WO2011086447A3 (en) Fermentation process for the preparation of recombinant heterologous proteins
WO2012150976A3 (en) Methods and compositions for the production of extremophile enzymes from green microalgae and cyanobacteria
WO2010105257A3 (en) Generation of mouse induced pluripotent stem cells bytransient expression of a single non- viral polycistronic vector
HK1198657A1 (en) Addition of iron to improve cell culture

Legal Events

Date Code Title Description
TH4A Publication of the corrected specification to eurasian patent
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ KG MD TJ TM