DK3354732T3 - Nukleasemedieret dna-samling - Google Patents

Nukleasemedieret dna-samling Download PDF

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Publication number
DK3354732T3
DK3354732T3 DK18162656.5T DK18162656T DK3354732T3 DK 3354732 T3 DK3354732 T3 DK 3354732T3 DK 18162656 T DK18162656 T DK 18162656T DK 3354732 T3 DK3354732 T3 DK 3354732T3
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Prior art keywords
mediated dna
dna collection
nuclease mediated
nuclease
collection
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DK18162656.5T
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Inventor
Chris Schoenherr
John Mcwhirter
Corey Momont
Lynn Macdonald
Andrew Murphy
Gregg Warshaw
Jose Rojas
Ka-Man Lai
David Valenzuela
Caitlin Montagna
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Regeneron Pharma
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK18162656.5T 2014-06-23 2015-06-23 Nukleasemedieret dna-samling DK3354732T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462015809P 2014-06-23 2014-06-23
US201462016400P 2014-06-24 2014-06-24
US201462036983P 2014-08-13 2014-08-13
EP15735807.8A EP3155099B1 (en) 2014-06-23 2015-06-23 Nuclease-mediated dna assembly

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DK3354732T3 true DK3354732T3 (da) 2020-04-06

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DK15735807.8T DK3155099T3 (da) 2014-06-23 2015-06-23 Nukleasemedieret dna-samling

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US (5) US9738897B2 (da)
EP (3) EP3708663A1 (da)
JP (4) JP6336140B2 (da)
KR (2) KR102237151B1 (da)
CN (2) CN106715694B (da)
AU (3) AU2015280120B2 (da)
BR (1) BR112016030145A8 (da)
CA (1) CA2953499C (da)
CY (2) CY1120520T1 (da)
DK (2) DK3354732T3 (da)
ES (2) ES2781323T3 (da)
HK (1) HK1256688A1 (da)
HR (1) HRP20200523T1 (da)
HU (1) HUE049405T2 (da)
IL (1) IL249612B (da)
LT (1) LT3354732T (da)
MX (2) MX2016016905A (da)
NZ (1) NZ765591A (da)
PL (2) PL3155099T3 (da)
PT (2) PT3155099T (da)
RS (1) RS60366B1 (da)
RU (1) RU2707911C2 (da)
SG (2) SG10201803444YA (da)
SI (1) SI3354732T1 (da)
WO (1) WO2015200334A1 (da)

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