BR112016030145A2 - ?métodos para a montagem de ao menos dois ácidos nucleicos e de dois ou mais ácidos nucleicos? - Google Patents
?métodos para a montagem de ao menos dois ácidos nucleicos e de dois ou mais ácidos nucleicos?Info
- Publication number
- BR112016030145A2 BR112016030145A2 BR112016030145-5A BR112016030145A BR112016030145A2 BR 112016030145 A2 BR112016030145 A2 BR 112016030145A2 BR 112016030145 A BR112016030145 A BR 112016030145A BR 112016030145 A2 BR112016030145 A2 BR 112016030145A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acids
- sequences
- create
- assembling
- methods
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 230000000295 complement effect Effects 0.000 abstract 3
- 101710163270 Nuclease Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 230000005782 double-strand break Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
a presente invenção está relacionada aos métodos para a montagem de ao menos dois ácidos nucleicos usando um agente nuclease sequência-específico (por exemplo, um complexo grna-cas) para criar sequências de extremidade tendo complementaridade e, subsequentemente, montar as sequências complementares de sobreposição. o agente nuclease (por exemplo, um complexo grna-cas) pode criar quebras na dupla fita no dsdna para criar sequências com extremidade de sobreposição ou pode criar entalhes em cada fita para produzir sequências com extremidade saliente complementares. a montagem usando o método aqui descrito pode montar quaisquer ácidos nucleicos tendo sequências de sobreposição ou pode usar um oligo de união para montar sequências sem extremidades complementares.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462015809P | 2014-06-23 | 2014-06-23 | |
US62/015809 | 2014-06-23 | ||
US201462016400P | 2014-06-24 | 2014-06-24 | |
US62/016400 | 2014-06-24 | ||
US201462036983P | 2014-08-13 | 2014-08-13 | |
US62/036983 | 2014-08-13 | ||
PCT/US2015/037199 WO2015200334A1 (en) | 2014-06-23 | 2015-06-23 | Nuclease-mediated dna assembly |
Publications (2)
Publication Number | Publication Date |
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BR112016030145A2 true BR112016030145A2 (pt) | 2018-02-20 |
BR112016030145A8 BR112016030145A8 (pt) | 2018-12-11 |
Family
ID=53525285
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112016030145A BR112016030145A8 (pt) | 2014-06-23 | 2015-06-23 | métodos para a montagem de ao menos dois ácidos nucleicos e de dois ou mais ácidos nucleicos |
Country Status (25)
Country | Link |
---|---|
US (5) | US9738897B2 (pt) |
EP (3) | EP3708663A1 (pt) |
JP (4) | JP6336140B2 (pt) |
KR (2) | KR102237151B1 (pt) |
CN (2) | CN106715694B (pt) |
AU (3) | AU2015280120B2 (pt) |
BR (1) | BR112016030145A8 (pt) |
CA (1) | CA2953499C (pt) |
CY (2) | CY1120520T1 (pt) |
DK (2) | DK3354732T3 (pt) |
ES (2) | ES2781323T3 (pt) |
HK (1) | HK1256688A1 (pt) |
HR (1) | HRP20200523T1 (pt) |
HU (1) | HUE049405T2 (pt) |
IL (1) | IL249612B (pt) |
LT (1) | LT3354732T (pt) |
MX (2) | MX2016016905A (pt) |
NZ (1) | NZ765591A (pt) |
PL (2) | PL3155099T3 (pt) |
PT (2) | PT3155099T (pt) |
RS (1) | RS60366B1 (pt) |
RU (1) | RU2707911C2 (pt) |
SG (2) | SG10201803444YA (pt) |
SI (1) | SI3354732T1 (pt) |
WO (1) | WO2015200334A1 (pt) |
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