DK282287A - Fremgangsmaade til isolering af biologisk aktivt, monomert vaeksthormon - Google Patents
Fremgangsmaade til isolering af biologisk aktivt, monomert vaeksthormonInfo
- Publication number
- DK282287A DK282287A DK282287A DK282287A DK282287A DK 282287 A DK282287 A DK 282287A DK 282287 A DK282287 A DK 282287A DK 282287 A DK282287 A DK 282287A DK 282287 A DK282287 A DK 282287A
- Authority
- DK
- Denmark
- Prior art keywords
- growth hormone
- monomerative
- insulating
- procedure
- biological active
- Prior art date
Links
- 102000018997 Growth Hormone Human genes 0.000 abstract 5
- 108010051696 Growth Hormone Proteins 0.000 abstract 5
- 239000000122 growth hormone Substances 0.000 abstract 5
- 150000002357 guanidines Chemical class 0.000 abstract 2
- 210000003000 inclusion body Anatomy 0.000 abstract 2
- 239000012266 salt solution Substances 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 229960000789 guanidine hydrochloride Drugs 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 230000003381 solubilizing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/869,702 US4705848A (en) | 1986-06-02 | 1986-06-02 | Isolation of bioactive, monomeric growth hormone |
Publications (2)
Publication Number | Publication Date |
---|---|
DK282287D0 DK282287D0 (da) | 1987-06-02 |
DK282287A true DK282287A (da) | 1987-12-03 |
Family
ID=25354104
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK282287A DK282287A (da) | 1986-06-02 | 1987-06-02 | Fremgangsmaade til isolering af biologisk aktivt, monomert vaeksthormon |
Country Status (12)
Country | Link |
---|---|
US (1) | US4705848A (ja) |
EP (1) | EP0248618B1 (ja) |
JP (1) | JPH0822877B2 (ja) |
KR (1) | KR960003548B1 (ja) |
AT (1) | ATE86627T1 (ja) |
CA (1) | CA1273592A (ja) |
DE (1) | DE3784555T2 (ja) |
DK (1) | DK282287A (ja) |
ES (1) | ES2053537T3 (ja) |
IE (1) | IE61977B1 (ja) |
IL (1) | IL82735A0 (ja) |
MX (1) | MX174060B (ja) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5453363A (en) * | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
GB8701848D0 (en) * | 1987-01-28 | 1987-03-04 | Celltech Ltd | Polypeptides |
US4975529A (en) * | 1989-08-18 | 1990-12-04 | Monsanto Company | Method of folding somatotropins |
DK580789D0 (da) * | 1989-11-20 | 1989-11-20 | Novo Nordisk As | Fremgangsmaade til oprensning af polypeptider |
US5288931A (en) * | 1991-12-06 | 1994-02-22 | Genentech, Inc. | Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation |
US5663304A (en) * | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
EP0840745A2 (en) * | 1995-07-21 | 1998-05-13 | Amgen Inc. | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt |
KR970006498A (ko) * | 1995-07-31 | 1997-02-21 | 유충식 | 활성형 인성장호르몬의 정제 방법 |
US5912327A (en) * | 1996-09-30 | 1999-06-15 | Human Genome Sciences, Inc. | Method of purifying chemokines from inclusion bodies |
US6632425B1 (en) | 1997-03-20 | 2003-10-14 | Human Genome Sciences, Inc. | Chemokine compositions |
JP4798832B2 (ja) * | 2000-10-24 | 2011-10-19 | 一般財団法人化学及血清療法研究所 | ヒト血清アルブミン多量体の除去方法 |
EP1329460B1 (en) * | 2000-10-24 | 2006-06-28 | Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute | Method of monomerizing human serum albumin polymers |
CA3144697A1 (en) * | 2010-11-12 | 2012-05-18 | Nektar Therapeutics | Conjugates of an il-2 moiety and a polymer |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU553017B2 (en) * | 1982-06-07 | 1986-06-26 | Chr. Hansen A/S | A process for the preparation of chymosin |
IE56372B1 (en) * | 1982-12-22 | 1991-07-03 | Genentech Inc | Microbially produced rennet methods for its production and plasmids used for its production |
US4511502A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4512922A (en) * | 1982-12-22 | 1985-04-23 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4511503A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
GR79124B (ja) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
US4518526A (en) * | 1982-12-22 | 1985-05-21 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
JPS59166086A (ja) * | 1983-03-09 | 1984-09-19 | Teruhiko Beppu | 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 |
DE122080T1 (de) * | 1983-03-25 | 1985-05-23 | Celltech Ltd., Slough, Berkshire | Verfahren zur herstellung eines proteins. |
EP0123928A3 (en) * | 1983-03-31 | 1986-02-05 | Codon Genetic Engineering Laboratories | Recombinant dna coding for a polypeptide displaying milk clotting activity |
US4788144A (en) * | 1985-06-28 | 1988-11-29 | International Minerals & Chemical Corp. | Fermentation process for the high level production of swine growth |
US4766224A (en) * | 1985-08-19 | 1988-08-23 | International Minerals & Chemical Corp. | Purification and activation of proteins from insoluble inclusion bodies |
US4656255A (en) * | 1985-09-09 | 1987-04-07 | International Minerals & Chemical Corp. | Protein recovery |
-
1986
- 1986-06-02 US US06/869,702 patent/US4705848A/en not_active Expired - Lifetime
-
1987
- 1987-05-29 IE IE142287A patent/IE61977B1/en not_active IP Right Cessation
- 1987-06-01 ES ES87304818T patent/ES2053537T3/es not_active Expired - Lifetime
- 1987-06-01 AT AT87304818T patent/ATE86627T1/de not_active IP Right Cessation
- 1987-06-01 JP JP62134993A patent/JPH0822877B2/ja not_active Expired - Lifetime
- 1987-06-01 KR KR1019870005507A patent/KR960003548B1/ko not_active IP Right Cessation
- 1987-06-01 DE DE8787304818T patent/DE3784555T2/de not_active Expired - Fee Related
- 1987-06-01 EP EP87304818A patent/EP0248618B1/en not_active Expired - Lifetime
- 1987-06-01 IL IL82735A patent/IL82735A0/xx not_active IP Right Cessation
- 1987-06-02 DK DK282287A patent/DK282287A/da not_active Application Discontinuation
- 1987-06-02 CA CA000538594A patent/CA1273592A/en not_active Expired - Lifetime
- 1987-06-02 MX MX027073A patent/MX174060B/es unknown
Also Published As
Publication number | Publication date |
---|---|
ATE86627T1 (de) | 1993-03-15 |
DE3784555D1 (de) | 1993-04-15 |
US4705848A (en) | 1987-11-10 |
EP0248618A3 (en) | 1990-02-07 |
JPH0822877B2 (ja) | 1996-03-06 |
MX174060B (es) | 1994-04-18 |
DE3784555T2 (de) | 1993-07-01 |
KR880000578A (ko) | 1988-03-26 |
CA1273592C (en) | 1990-09-04 |
IL82735A0 (en) | 1987-12-20 |
ES2053537T3 (es) | 1994-08-01 |
EP0248618A2 (en) | 1987-12-09 |
KR960003548B1 (ko) | 1996-03-15 |
IE61977B1 (en) | 1994-12-14 |
IE871422L (en) | 1987-12-02 |
EP0248618B1 (en) | 1993-03-10 |
JPS63258492A (ja) | 1988-10-25 |
DK282287D0 (da) | 1987-06-02 |
CA1273592A (en) | 1990-09-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK282287D0 (da) | Fremgangsmaade til isolering af biologisk aktivt, monomert vaeksthormon | |
DK394786D0 (da) | Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer | |
KR870004056A (ko) | 단백질 아미노-종결잔기의 선택적인 화학적 제거방법 | |
DK430486A (da) | Fremgangsmaade til udvinding af protein | |
DE3689870D1 (de) | Formulierung für rekombinantes beta-Interferon, Verfahren zur Gewinnung und Stabilisierung des beta-Interferons und dessen Verwendung. | |
DK426386A (da) | Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer | |
DE3686954D1 (de) | Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate. | |
ATE43639T1 (de) | Verfahren zur entfernung von verunreinigungen aus leukozytinterferon-zubereitungen und das so hergestellte gereinigte leukozytinterferon. | |
ATE111480T1 (de) | Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln. | |
DE3855986D1 (de) | Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen | |
DK164916C (da) | Fremgangsmaade til rensning eller berigelse af biologisk aktive proteiner og hertil egnet middel | |
ATE96805T1 (de) | Verfahren zur rueckgewinnung rekombinanter proteine. | |
RU93031156A (ru) | Способ получения плазмид, кодирующих химерный белок с последовательностью соматостатина, штамм бактерий escherichia coli - продуцент этого белка, белок, полученный в этом штамме, содержащий фрагмент ацетилтрансферазы, спейсер и иммуногенный соматостатин | |
DE3880122D1 (de) | Verfahren zur solubilisierung von unloeslichem protein. | |
KR900007865A (ko) | 융합 단백질의 선택적 절단 방법 | |
NO911609L (no) | Veksthormon-frigjoerende faktoranaloger. | |
KR940014780A (ko) | 성장호르몬의 봉입체로부터 활성형태의 성장호르몬을 제조하는 방법 | |
ITRM920385A1 (it) | Procedimento per la purificazione di proteine da sistemi cellulari. | |
AR008364A1 (es) | Procedimiento para la purificacion de eritropoyetina humana recombinante a partir de sobrenadantes de cultivo de celulas y eritropoyetina humanarecombinante obtenida con tal procedimiento |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AHS | Application shelved for other reasons than non-payment |