MX174060B - Aislamiento de hormona del crecimiento monomerica, activa - Google Patents

Aislamiento de hormona del crecimiento monomerica, activa

Info

Publication number
MX174060B
MX174060B MX027073A MX2707387A MX174060B MX 174060 B MX174060 B MX 174060B MX 027073 A MX027073 A MX 027073A MX 2707387 A MX2707387 A MX 2707387A MX 174060 B MX174060 B MX 174060B
Authority
MX
Mexico
Prior art keywords
growth hormone
monomeric
insulation
active growth
solution
Prior art date
Application number
MX027073A
Other languages
English (en)
Inventor
Ren-Der Yang
Edwin J Hamilton Jr
Larry D Taber
Original Assignee
Int Minerals & Chem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Minerals & Chem Corp filed Critical Int Minerals & Chem Corp
Publication of MX174060B publication Critical patent/MX174060B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a un procedimiento para recuperar y purificar hormona del crecimiento monomerica, biológicamente activa, de cuerpos de inclusión insolubles producidos por expresión de un gene heterólogo en un microorganismo, caracterizado porque comprende: a) solubilizar y desnaturalizar la hormona del crecimiento extrayendo los cuerpos de inclusión en una solución de una sal de guanidina; b) renaturalizar cuando menos una porción de la hormona del crecimiento en la solución, reemplazando la solución de sal de guanidina por una solución reguladora libre de desnaturalizante, sin el uso de un desnaturalizante intermedio; c) separar los contaminantes precipitados y agregados de la solución reguladora libre de desnaturalizante; y d) purificar la hormona del crecimiento monomerica en la solución, mediante cromatografía de cambio de iones.
MX027073A 1986-06-02 1987-06-02 Aislamiento de hormona del crecimiento monomerica, activa MX174060B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/869,702 US4705848A (en) 1986-06-02 1986-06-02 Isolation of bioactive, monomeric growth hormone

Publications (1)

Publication Number Publication Date
MX174060B true MX174060B (es) 1994-04-18

Family

ID=25354104

Family Applications (1)

Application Number Title Priority Date Filing Date
MX027073A MX174060B (es) 1986-06-02 1987-06-02 Aislamiento de hormona del crecimiento monomerica, activa

Country Status (12)

Country Link
US (1) US4705848A (es)
EP (1) EP0248618B1 (es)
JP (1) JPH0822877B2 (es)
KR (1) KR960003548B1 (es)
AT (1) ATE86627T1 (es)
CA (1) CA1273592C (es)
DE (1) DE3784555T2 (es)
DK (1) DK282287A (es)
ES (1) ES2053537T3 (es)
IE (1) IE61977B1 (es)
IL (1) IL82735A0 (es)
MX (1) MX174060B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
WO1997004003A2 (en) * 1995-07-21 1997-02-06 Amgen Inc. Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts
KR970006498A (ko) * 1995-07-31 1997-02-21 유충식 활성형 인성장호르몬의 정제 방법
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
US6632425B1 (en) 1997-03-20 2003-10-14 Human Genome Sciences, Inc. Chemokine compositions
ES2261487T3 (es) * 2000-10-24 2006-11-16 Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute Metodo de monomerizacion de polimeros de albumina de suero humano.
JP4798832B2 (ja) * 2000-10-24 2011-10-19 一般財団法人化学及血清療法研究所 ヒト血清アルブミン多量体の除去方法
HUE054318T2 (hu) * 2010-11-12 2021-08-30 Nektar Therapeutics IL-2 molekularész konjugátumai és polimer

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2129810B (en) * 1982-06-07 1985-09-18 Celltech Ltd A process for the preparation of chymosin
GR79124B (es) * 1982-12-22 1984-10-02 Genentech Inc
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
WO1984003711A1 (en) * 1983-03-25 1984-09-27 Celltech Ltd A process for the production of a protein
FI841288A (fi) * 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant-dna-kod foer uttryckning av polypeptid.
US4788144A (en) * 1985-06-28 1988-11-29 International Minerals & Chemical Corp. Fermentation process for the high level production of swine growth
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery

Also Published As

Publication number Publication date
ATE86627T1 (de) 1993-03-15
JPS63258492A (ja) 1988-10-25
JPH0822877B2 (ja) 1996-03-06
DK282287A (da) 1987-12-03
US4705848A (en) 1987-11-10
CA1273592A (en) 1990-09-04
KR880000578A (ko) 1988-03-26
IE61977B1 (en) 1994-12-14
EP0248618B1 (en) 1993-03-10
DK282287D0 (da) 1987-06-02
DE3784555T2 (de) 1993-07-01
IE871422L (en) 1987-12-02
DE3784555D1 (de) 1993-04-15
ES2053537T3 (es) 1994-08-01
CA1273592C (en) 1990-09-04
EP0248618A2 (en) 1987-12-09
KR960003548B1 (ko) 1996-03-15
IL82735A0 (en) 1987-12-20
EP0248618A3 (en) 1990-02-07

Similar Documents

Publication Publication Date Title
MX174060B (es) Aislamiento de hormona del crecimiento monomerica, activa
BR8603944A (pt) Processo de purificacao e ativacao de proteinas a partir de corpos de inclusao insoluveis
ATE113960T1 (de) Proteingewinnung.
DE3686954D1 (de) Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate.
ATE43639T1 (de) Verfahren zur entfernung von verunreinigungen aus leukozytinterferon-zubereitungen und das so hergestellte gereinigte leukozytinterferon.
CN1025350C (zh) 促进变性剂溶液中所含的重组蛋白形成分子内二硫键的方法
ATE156491T1 (de) Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
DE69121742D1 (de) Verfahren zur Herstellung von Somatotropinmonomeren in reiner Form
AU631356B2 (en) Improved process for recovering microbially produced interferon-beta
EP0085220A3 (en) Method for purification of anterior pituitary hormones
EP0446850A3 (en) Process for purifying recombinant human beta-interferon
KR940014780A (ko) 성장호르몬의 봉입체로부터 활성형태의 성장호르몬을 제조하는 방법