KR900007865A - 융합 단백질의 선택적 절단 방법 - Google Patents
융합 단백질의 선택적 절단 방법 Download PDFInfo
- Publication number
- KR900007865A KR900007865A KR1019890015850A KR890015850A KR900007865A KR 900007865 A KR900007865 A KR 900007865A KR 1019890015850 A KR1019890015850 A KR 1019890015850A KR 890015850 A KR890015850 A KR 890015850A KR 900007865 A KR900007865 A KR 900007865A
- Authority
- KR
- South Korea
- Prior art keywords
- fusion protein
- formula
- type
- trypsin
- endopeptidase
- Prior art date
Links
- 102000037865 fusion proteins Human genes 0.000 title claims abstract 9
- 108020001507 fusion proteins Proteins 0.000 title claims abstract 9
- 238000003776 cleavage reaction Methods 0.000 title claims abstract 5
- 230000007017 scission Effects 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims 9
- 101710171801 Alpha-amylase inhibitor Proteins 0.000 claims abstract 4
- 108010059378 Endopeptidases Proteins 0.000 claims abstract 4
- 102000005593 Endopeptidases Human genes 0.000 claims abstract 4
- 239000003392 amylase inhibitor Substances 0.000 claims abstract 4
- 230000002255 enzymatic effect Effects 0.000 claims abstract 4
- 229920001184 polypeptide Polymers 0.000 claims abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- 238000002425 crystallisation Methods 0.000 claims abstract 2
- 150000001875 compounds Chemical class 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 108010053229 Lysyl endopeptidase Proteins 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000008025 crystallization Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 239000004026 insulin derivative Substances 0.000 abstract 2
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- -1 for example Proteins 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 230000001810 trypsinlike Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Welding Or Cutting Using Electron Beams (AREA)
- Fuses (AREA)
- Design And Manufacture Of Integrated Circuits (AREA)
- Semiconductor Integrated Circuits (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (8)
- 융합 단백질로서 하기 서열식(Ⅰ)의 화합물을 사용함을 특징으로 하여 트립신-형 엔도펩티다제를 사용한 융합 단백질의 효소적 절단에 의해 폴리펩타이드 또는 단백질을 제조하는 방법.상기식에서, R1은 -(AA)0-(펩타이드-형 α-아밀라아제 억제제)이고, 여기에서 AA는 Lys을 제외한 유전적으로 암호화가능한 아미노산이고, 0는 0 내지 200, 바람직하게는 5 내지 100, 특히 10 내지 12의 정수이며, 펩타이드-형 α-아밀라제 억제제는 문헌에 공지된 α-아밀라제 억제제의 잔기이고, P는 목적하는 폴리펩타이드 또는 단백질이고; R2는 -(AA)p-이고, 여기에서, AA는 R에서 정의한 것과 같고(Lys를 제외한 유전적으로 암호화 가능한 아미노산), p는 1 내지 35, 바람직하게는 1 내지 10, 특히 1 내지 3의 정수이고; m은 0 또는 1≥n이다.
- 제1항에 있어서, R1이 단지 Ala 또는 Thr, 특히 단지 Thr로 이루어지며 m 및 n이 각각 1인 서열식(Ⅰ)의 화합물을 융합 단백질로서 사용하는 방법.
- 제1 또는 2항에 있어서, R1이 하기 서열식인 서열식(Ⅰ)의 화합물을 융합 단백질로서 사용하는 방법.
- 제1 내지 3항 중 어느 한 항에 있어서, 서열식(Ⅰ)의 융합 단백질을 총 반응 혼합물을 기준하여 약 0.02 내지 15중량%, 바람직하게는 약 0.5 내지 5중량%의 농도로 사용하는 방법.
- 제1 내지 4항 중 어느 한 항에 있어서, 리실 엔도펩티다제가 트립신-형 엔도펩티다제로서 사용되는 방법.
- 제1 내지 5항 중 어느 한 항에 있어서, 트립신-형 엔도펩티다제가 서열식(Ⅰ)의 융합 단밸질의 중량에 대해 약 1/5 내지의 농도로 사용되는 방법.
- 제1 내지 6항 중 어느 한 항에 있어서, 효소적 절단을 pH 5 내지 11, 바람직하게는 약 7 내지 9.5의 수용액중에, 경우에 따라 용해제의 존재하에 수행하는 방법.
- 제1 내지 7항 중 어느 한 항에 있어서, 반응 혼합물을 효소적 절단이 완료된 후 결정화로 후처리하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3837271.1 | 1987-11-03 | ||
| DE3837271A DE3837271A1 (de) | 1988-11-03 | 1988-11-03 | Verfahren zur selektiven spaltung von fusionsproteinen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR900007865A true KR900007865A (ko) | 1990-06-02 |
Family
ID=6366365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890015850A KR900007865A (ko) | 1987-11-03 | 1989-11-02 | 융합 단백질의 선택적 절단 방법 |
Country Status (17)
| Country | Link |
|---|---|
| EP (1) | EP0367161B1 (ko) |
| JP (1) | JP2877253B2 (ko) |
| KR (1) | KR900007865A (ko) |
| AT (1) | ATE139538T1 (ko) |
| AU (1) | AU618420B2 (ko) |
| DE (2) | DE3837271A1 (ko) |
| DK (1) | DK546689A (ko) |
| ES (1) | ES2088883T3 (ko) |
| FI (1) | FI895183A0 (ko) |
| GR (1) | GR3020297T3 (ko) |
| HU (1) | HUT53677A (ko) |
| IL (1) | IL92179A0 (ko) |
| NO (1) | NO894362L (ko) |
| NZ (1) | NZ231221A (ko) |
| PH (1) | PH26508A (ko) |
| PT (1) | PT92176B (ko) |
| ZA (1) | ZA898376B (ko) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012048856A1 (en) | 2010-10-12 | 2012-04-19 | Glucometrix Pvs Gmbh | Proinsulin with helper sequence |
| WO2012098009A1 (en) | 2011-01-21 | 2012-07-26 | Glucometrix Ag | Chimeric polypeptide comprising a membrane protein and an insulin precursor |
| EP2867250A2 (en) | 2012-04-04 | 2015-05-06 | GlucoMetrix AG | Proinsulin with enhanced helper sequence |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3418274A1 (de) * | 1984-05-17 | 1985-11-21 | Hoechst Ag, 6230 Frankfurt | Signalpeptid fuer die exkretion von peptiden in streptomyceten |
| DK129385A (da) * | 1985-03-22 | 1986-09-23 | Novo Industri As | Peptider og fremstilling deraf |
| DE3707150A1 (de) * | 1987-03-06 | 1988-09-15 | Hoechst Ag | Tendamistat-derivate |
-
1988
- 1988-11-03 DE DE3837271A patent/DE3837271A1/de not_active Withdrawn
-
1989
- 1989-10-28 ES ES89120054T patent/ES2088883T3/es not_active Expired - Lifetime
- 1989-10-28 EP EP89120054A patent/EP0367161B1/de not_active Expired - Lifetime
- 1989-10-28 AT AT89120054T patent/ATE139538T1/de not_active IP Right Cessation
- 1989-10-28 DE DE58909692T patent/DE58909692D1/de not_active Expired - Lifetime
- 1989-11-01 NZ NZ231221A patent/NZ231221A/xx unknown
- 1989-11-01 IL IL92179A patent/IL92179A0/xx unknown
- 1989-11-01 FI FI895183A patent/FI895183A0/fi not_active Application Discontinuation
- 1989-11-02 PT PT92176A patent/PT92176B/pt not_active IP Right Cessation
- 1989-11-02 JP JP1285089A patent/JP2877253B2/ja not_active Expired - Lifetime
- 1989-11-02 PH PH39449A patent/PH26508A/en unknown
- 1989-11-02 NO NO89894362A patent/NO894362L/no unknown
- 1989-11-02 KR KR1019890015850A patent/KR900007865A/ko not_active Application Discontinuation
- 1989-11-02 DK DK546689A patent/DK546689A/da not_active Application Discontinuation
- 1989-11-02 AU AU43977/89A patent/AU618420B2/en not_active Expired - Fee Related
- 1989-11-03 ZA ZA898376A patent/ZA898376B/xx unknown
- 1989-11-03 HU HU895664A patent/HUT53677A/hu unknown
-
1996
- 1996-06-20 GR GR960401606T patent/GR3020297T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL92179A0 (en) | 1990-07-26 |
| HU895664D0 (en) | 1990-01-28 |
| AU618420B2 (en) | 1991-12-19 |
| DE58909692D1 (de) | 1996-07-25 |
| ATE139538T1 (de) | 1996-07-15 |
| DK546689A (da) | 1990-05-04 |
| NO894362L (no) | 1990-05-04 |
| EP0367161A2 (de) | 1990-05-09 |
| ES2088883T3 (es) | 1996-10-01 |
| HUT53677A (en) | 1990-11-28 |
| NO894362D0 (no) | 1989-11-02 |
| AU4397789A (en) | 1990-05-10 |
| JPH02195896A (ja) | 1990-08-02 |
| DK546689D0 (da) | 1989-11-02 |
| EP0367161A3 (de) | 1991-09-25 |
| ZA898376B (en) | 1990-08-29 |
| NZ231221A (en) | 1991-10-25 |
| DE3837271A1 (de) | 1990-05-10 |
| EP0367161B1 (de) | 1996-06-19 |
| JP2877253B2 (ja) | 1999-03-31 |
| PH26508A (en) | 1992-08-07 |
| PT92176A (pt) | 1990-05-31 |
| FI895183A0 (fi) | 1989-11-01 |
| PT92176B (pt) | 1995-07-03 |
| GR3020297T3 (en) | 1996-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |