KR840004169A - 프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법 - Google Patents

프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법 Download PDF

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KR840004169A
KR840004169A KR1019830000978A KR830000978A KR840004169A KR 840004169 A KR840004169 A KR 840004169A KR 1019830000978 A KR1019830000978 A KR 1019830000978A KR 830000978 A KR830000978 A KR 830000978A KR 840004169 A KR840004169 A KR 840004169A
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derivative
amino acid
carried out
ratio
acid ester
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KR1019830000978A
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KR900003502B1 (ko
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오베르마이어(외 2) 라이너
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하인리히 벡커,베른하르트 베크
훽스트 아크티엔 게젤샤프트
베른하르트 베크
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/26—Containing cys-cys disulfide bridge between nonadjacent cysteine residues

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 프로인슐린 전구물질 유사체를 트립신 또는 트립신-형 엔도펩티다제 존재하의 인슐린 등전점 미만의 pH에서 천연 아미노산의 에스테르 또는 천연 아미노산 에스테르의 유도체와 반응시킨 다음, 에스테르기와 경우에 따라서 존재할 수도 있는 다른 보호기물을 제거시킴을 특징으로 하는 프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법.
  2. 제 1 항에 있어서, 아미노산 유도체가 ala-OtBu 또는 thr(tBu)OtBu인 방법.
  3. 제 1 항에 있어서, pH 3.8 내지 5.5에서 수행하는 방법.
  4. 제 1 항에 있어서, pH3.8 내지 5.0에서 수행하는 방법.
  5. 제 1 항에 있어서, 효소/기질 비율이 1 : 1 내지 1 : 100 중량비인 방법.
  6. 제 1 항에 있어서, 효소/기질 비율이 1 : 4 내지 1 : 10 중량비인 방법.
  7. 제 1 항에 있어서, 50 내지 500배몰 과잉의 아미노산 에스테르 또는 그 유도체의 존재하에 수행하는 방법.
  8. 제 1 항에 있어서, 100 내지 250배몰 과잉의 아이노산 에스테르 또는 그 유도체의 존재하에 수행하는방법.
  9. 제 1 항에 있어서, 반응온도가 +2내지 +37℃인 방법.
  10. 제 1 항에 있어서, 반응온도가 +4내지 +10℃인 방법.
  11. 제 1 항에 있어서, pH 3.8 내지 5.5, 효소/기질의 비율 1 : 1 내지 1 : 100 중량비, 반응온도 +2 내지 +37℃ 및 50 내지 500배몰 과잉의 아미노산 에스테르 또는 그의 유도체를 사용하여 반응을 수행하는 방법.
  12. 제 1 항에 있어서, pH3.8 내지 5.0, 효소/기질의 비율 1 : 4 내지 1 : 10 중량비: 반응온도 +4 내지 +10℃ 및 100 내지 250배몰 과잉의 아미노산 에스테르 또는 그의 유도체의 존재하에 반응을 수행하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019830000978A 1982-03-13 1983-03-11 프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법 KR900003502B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3209184.2 1982-03-13
DE19823209184 DE3209184A1 (de) 1982-03-13 1982-03-13 Verfahren zur umwandlung von praeproinsulinanaloga zu insulinen

Publications (2)

Publication Number Publication Date
KR840004169A true KR840004169A (ko) 1984-10-10
KR900003502B1 KR900003502B1 (ko) 1990-05-21

Family

ID=6158163

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019830000978A KR900003502B1 (ko) 1982-03-13 1983-03-11 프로인슐린 전구물질 유사체로부터 인슐린을 제조하는 방법

Country Status (15)

Country Link
US (1) US4639333A (ko)
EP (1) EP0089007B1 (ko)
JP (1) JPS58190398A (ko)
KR (1) KR900003502B1 (ko)
AT (1) ATE21118T1 (ko)
AU (1) AU553832B2 (ko)
CA (1) CA1195273A (ko)
DE (2) DE3209184A1 (ko)
DK (1) DK154573C (ko)
ES (1) ES8401448A1 (ko)
FI (1) FI79117C (ko)
GR (1) GR78453B (ko)
IE (1) IE54605B1 (ko)
IL (1) IL68103A (ko)
ZA (1) ZA831693B (ko)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3326472A1 (de) * 1983-07-22 1985-02-14 Hoechst Ag, 6230 Frankfurt Neue insulin-derivate, verfahren zu deren herstellung und deren verwendung sowie pharmazeutische mittel zur behandlung des diabetes mellitus
DE3333640A1 (de) * 1983-09-17 1985-04-25 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von insulin-derivaten, deren b-kette c-terminal verlaengert ist, neue basisch modifizierte insulin-derivate diese enthaltende mittel und ihre verwendung
US4569791A (en) * 1984-06-14 1986-02-11 Eli Lilly And Company Anti-diabetic compounds
US4569792A (en) * 1984-06-14 1986-02-11 Eli Lilly And Company Anti-diabetic compounds
US4581165A (en) * 1984-06-14 1986-04-08 Eli Lilly And Company Anti-diabetic compounds
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
NZ223630A (en) * 1987-02-25 1989-11-28 Novo Industri As Insulin derivative and pharmaceutical compositions
US5514646A (en) * 1989-02-09 1996-05-07 Chance; Ronald E. Insulin analogs modified at position 29 of the B chain
NZ239652A (en) * 1990-09-05 1992-09-25 Hoechst Ag Hydrolysis of preproinsulin to insulin and analogues using clostripain and optionally carboxypeptidase
RU2458989C1 (ru) 2008-08-07 2012-08-20 Байокон Лимитид Способ получения аналогов инсулина из их соответствующих предшественников (варианты)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3276961A (en) * 1963-04-08 1966-10-04 Squibb & Sons Inc Process for preparing human insulin
GB1426061A (en) * 1972-12-28 1976-02-25 Ici Ltd Polypeptides
US3903068A (en) * 1972-12-26 1975-09-02 Us Health Education & Welfare Facile synthesis of human insulin by modification of porcine insulin
NO153061C (no) * 1979-04-13 1986-01-08 Shionogi & Co Fremgangsmaate for fremstilling av et b30-threonininsulin
JPS55138393A (en) * 1979-04-13 1980-10-29 Shionogi & Co Ltd Semisynthesis of insulin
DK147437A (en) * 1980-02-11 1900-01-01 Process for preparing human insulin or threonine B30 esters of human insulin, or a salt or complex thereof
PH16156A (en) * 1980-03-27 1983-07-18 Lilly Co Eli Process for producing an insulin precursor
DK319780A (da) * 1980-07-24 1982-01-25 Forenede Bryggerier As Fremgangsmaade til enzymatisk udskiftning af b-30 aminosyren i insuliner
DE3101382A1 (de) * 1981-01-17 1982-09-02 Hoechst Ag, 6000 Frankfurt "verfahren zur herstellung von humanisulin oder dessen derivaten aus schweineinsulin oder dessen derivaten"

Also Published As

Publication number Publication date
DK117883A (da) 1983-09-14
ZA831693B (en) 1983-11-30
JPH0536034B2 (ko) 1993-05-28
IE830529L (en) 1983-09-13
CA1195273A (en) 1985-10-15
AU553832B2 (en) 1986-07-31
FI830813L (fi) 1983-09-14
FI830813A0 (fi) 1983-03-10
ATE21118T1 (de) 1986-08-15
FI79117B (fi) 1989-07-31
ES520493A0 (es) 1983-12-16
EP0089007A1 (de) 1983-09-21
US4639333A (en) 1987-01-27
AU1242883A (en) 1983-09-15
FI79117C (fi) 1989-11-10
KR900003502B1 (ko) 1990-05-21
DK154573C (da) 1989-05-22
DE3364838D1 (en) 1986-09-04
IE54605B1 (en) 1989-12-06
ES8401448A1 (es) 1983-12-16
DK154573B (da) 1988-11-28
GR78453B (ko) 1984-09-27
JPS58190398A (ja) 1983-11-07
IL68103A (en) 1986-01-31
IL68103A0 (en) 1983-06-15
EP0089007B1 (de) 1986-07-30
DE3209184A1 (de) 1983-09-15
DK117883D0 (da) 1983-03-11

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