DE3855986D1 - Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen - Google Patents

Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen

Info

Publication number
DE3855986D1
DE3855986D1 DE3855986T DE3855986T DE3855986D1 DE 3855986 D1 DE3855986 D1 DE 3855986D1 DE 3855986 T DE3855986 T DE 3855986T DE 3855986 T DE3855986 T DE 3855986T DE 3855986 D1 DE3855986 D1 DE 3855986D1
Authority
DE
Germany
Prior art keywords
oxidized
microorganisms
guanidine hydrochloride
renatured
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3855986T
Other languages
English (en)
Other versions
DE3855986T2 (de
Inventor
Sidney Wolfe
Glenn Dorin
John Davis
Flint Smith
Amy Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Publication of DE3855986D1 publication Critical patent/DE3855986D1/de
Application granted granted Critical
Publication of DE3855986T2 publication Critical patent/DE3855986T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
DE3855986T 1987-05-11 1988-03-31 Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen Expired - Lifetime DE3855986T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4840887A 1987-05-11 1987-05-11
US16714488A 1988-03-25 1988-03-25
PCT/US1988/001043 WO1988008849A1 (en) 1987-05-11 1988-03-31 Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms

Publications (2)

Publication Number Publication Date
DE3855986D1 true DE3855986D1 (de) 1997-09-11
DE3855986T2 DE3855986T2 (de) 1998-02-05

Family

ID=26726091

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3855986T Expired - Lifetime DE3855986T2 (de) 1987-05-11 1988-03-31 Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen

Country Status (9)

Country Link
EP (1) EP0368857B1 (de)
JP (1) JP2955294B2 (de)
AT (1) ATE156491T1 (de)
AU (1) AU622860B2 (de)
DE (1) DE3855986T2 (de)
DK (1) DK10189A (de)
IL (1) IL86326A (de)
NO (1) NO176797C (de)
WO (1) WO1988008849A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
EP0337243A1 (de) * 1988-04-14 1989-10-18 F. Hoffmann-La Roche Ag Verfahren zur Reinigung von rekombinantem Interleukin-2
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
US5258496A (en) * 1990-07-10 1993-11-02 Scios Nova Inc. Isolation and purification of lung surfactant protein
AU658604B2 (en) * 1991-01-10 1995-04-27 Teijin Limited Highly sensitive assay of tissue factor and kit therefor
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
EP0663009A1 (de) * 1993-08-04 1995-07-19 Novartis AG Hochmolekulares desulphatohirudin
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
TW517059B (en) 1994-07-25 2003-01-11 Ciba Geigy Ag New process for the production of biologically active protein
JPH11509551A (ja) * 1995-07-21 1999-08-24 アムジエン・インコーポレーテツド 蛋白質を塩から分離することのできる連続静止相を用いてバッファー交換により蛋白質を巻き戻す方法
WO2003097669A2 (en) * 2002-05-17 2003-11-27 Københavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4572798A (en) * 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
JPH0640832B2 (ja) * 1985-05-10 1994-06-01 味の素株式会社 インタ−ロイキン２ポリペプチドの回収方法
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2

Also Published As

Publication number Publication date
NO885678D0 (no) 1988-12-21
EP0368857B1 (de) 1997-08-06
WO1988008849A1 (en) 1988-11-17
NO176797C (no) 1995-05-31
AU622860B2 (en) 1992-04-30
DK10189D0 (da) 1989-01-11
IL86326A (en) 1997-02-18
NO885678L (no) 1988-12-21
NO176797B (no) 1995-02-20
JP2955294B2 (ja) 1999-10-04
ATE156491T1 (de) 1997-08-15
DE3855986T2 (de) 1998-02-05
EP0368857A1 (de) 1990-05-23
AU1627988A (en) 1988-12-06
IL86326A0 (en) 1988-11-15
DK10189A (da) 1989-01-11
JPH02503384A (ja) 1990-10-18

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NOVARTIS VACCINES AND DIAGNOSTICS,INC.(N.D.GES, US