ATE111480T1 - Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln. - Google Patents
Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln.Info
- Publication number
- ATE111480T1 ATE111480T1 AT90912171T AT90912171T ATE111480T1 AT E111480 T1 ATE111480 T1 AT E111480T1 AT 90912171 T AT90912171 T AT 90912171T AT 90912171 T AT90912171 T AT 90912171T AT E111480 T1 ATE111480 T1 AT E111480T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- molecular weight
- high molecular
- flocculing
- agents
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 239000000356 contaminant Substances 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 1
- 239000012460 protein solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/399,021 US5047511A (en) | 1989-08-28 | 1989-08-28 | Method for recovering recombinant proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE111480T1 true ATE111480T1 (de) | 1994-09-15 |
Family
ID=23577800
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT90912171T ATE111480T1 (de) | 1989-08-28 | 1990-07-30 | Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5047511A (de) |
EP (1) | EP0494873B1 (de) |
AT (1) | ATE111480T1 (de) |
AU (1) | AU643456B2 (de) |
DE (1) | DE69012579T2 (de) |
NZ (1) | NZ235040A (de) |
WO (1) | WO1991003486A1 (de) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA94169B (en) * | 1993-01-26 | 1994-09-07 | Lilly Co Eli | Non-denaturing potency assay for bovine somatotropin |
WO1995032222A1 (en) * | 1994-05-19 | 1995-11-30 | Monsanto Company | Method for purifying somatotropin monomers |
KR100501817B1 (ko) * | 1998-07-21 | 2005-07-20 | 몬산토 테크놀로지 엘엘씨 | 음이온 폴리머성 응집제들을 사용한 단백질 침전물 현탁액의 청징화 방법 |
WO2003010188A1 (en) * | 2001-07-23 | 2003-02-06 | Natimmune A/S | Production of high molecular mass lectins |
US8123944B2 (en) * | 2001-08-28 | 2012-02-28 | Clearvalue Technologies, Inc. | Processes of aqueous solids treatment |
US7611700B2 (en) * | 2002-09-09 | 2009-11-03 | Hanall Pharmaceuticals, Co., Ltd. | Protease resistant modified interferon alpha polypeptides |
US7998930B2 (en) | 2004-11-04 | 2011-08-16 | Hanall Biopharma Co., Ltd. | Modified growth hormones |
AT501380B1 (de) * | 2005-02-07 | 2007-11-15 | Vitali Dipl Ing Dshemelinski | Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat |
FR2902799B1 (fr) | 2006-06-27 | 2012-10-26 | Millipore Corp | Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide |
US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
WO2008079280A1 (en) | 2006-12-21 | 2008-07-03 | Millipore Corporation | Purification of proteins |
US8569464B2 (en) | 2006-12-21 | 2013-10-29 | Emd Millipore Corporation | Purification of proteins |
US8999702B2 (en) * | 2008-06-11 | 2015-04-07 | Emd Millipore Corporation | Stirred tank bioreactor |
CN102257122B (zh) | 2008-12-16 | 2015-07-29 | Emd密理博公司 | 搅拌槽反应器及方法 |
SG184541A1 (en) | 2010-05-17 | 2012-11-29 | Emd Millipore Corp | Stimulus responsive polymers for the purification of biomolecules |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2175090A (en) * | 1938-06-22 | 1939-10-03 | Lederie Lab Inc | Purification of antibody compositions |
US3252961A (en) * | 1961-04-03 | 1966-05-24 | Foremost Dairies Inc | Whey process and product |
US3657071A (en) * | 1968-11-12 | 1972-04-18 | Hans Ulrich Bergmeyer | Fractionation of proteins |
DE2001902C3 (de) * | 1970-01-16 | 1978-10-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen |
US3749668A (en) * | 1971-05-03 | 1973-07-31 | Rohm & Haas | Water purification with amine oxide resins |
CH619433A5 (de) * | 1976-07-02 | 1980-09-30 | Ciba Geigy Ag | |
US4055469A (en) * | 1976-12-10 | 1977-10-25 | Eastman Kodak Company | Purification of microbial enzyme extracts using synthetic polyelectrolytes |
DE2718325C2 (de) * | 1977-04-25 | 1986-09-25 | Behringwerke Ag, 3550 Marburg | Leucinreiches 3,1-S-α↓2↓-Glykoprotein sowie dessen Verwendung |
US4204989A (en) * | 1978-12-13 | 1980-05-27 | Merck & Co., Inc. | Isolation of hepatitis B e antigen |
DE2946458A1 (de) * | 1979-11-17 | 1981-06-11 | Behringwerke Ag, 3550 Marburg | Neues protein pp(pfeil abwaerts)11(pfeil abwaerts) |
JPH0738880B2 (ja) * | 1983-06-24 | 1995-05-01 | 東レ株式会社 | エンドトキシン血症治療剤 |
US4634673A (en) * | 1984-03-28 | 1987-01-06 | Nabisco Brands, Inc. | Enzyme purification process |
JPS60248180A (ja) * | 1984-05-24 | 1985-12-07 | Showa Denko Kk | カリジノゲナ−ゼの精製方法およびその装置 |
DE3484713D1 (de) * | 1984-09-10 | 1991-07-18 | Toray Industries | Ionenaustausch- oder adsorptionsverfahren. |
US4645829A (en) * | 1984-10-29 | 1987-02-24 | Monsanto Company | Method for separating polypeptides |
DK111185A (da) * | 1985-03-12 | 1986-09-13 | Novo Industri As | Xyloseisomerase, fremgangsmaade til fremstilling deraf, immobiliseret xyloseisomerase og fremgangsmaade til isomerisering af glucose til fructose |
SE462100B (sv) * | 1985-08-08 | 1990-05-07 | Perstorp Ab | Komposition och dess anvaendning i ett tvaa- eller flerfassystem |
US4677196A (en) * | 1985-09-06 | 1987-06-30 | International Minerals & Chemical Corp. | Purification and activation of proteins from insoluble inclusion bodies |
DE3608091A1 (de) * | 1986-03-12 | 1987-09-17 | Basf Ag | Verfahren zum isolieren und reinigen von haemin |
US4771128A (en) * | 1986-10-10 | 1988-09-13 | Cetus Corporation | Method of purifying toxin conjugates using hydrophobic interaction chromatography |
US5101018A (en) * | 1989-06-12 | 1992-03-31 | International Minerals & Chemical Corp. | Method for recovering recombinant proteins |
-
1989
- 1989-08-28 US US07/399,021 patent/US5047511A/en not_active Expired - Lifetime
-
1990
- 1990-07-30 EP EP90912171A patent/EP0494873B1/de not_active Expired - Lifetime
- 1990-07-30 AU AU61715/90A patent/AU643456B2/en not_active Ceased
- 1990-07-30 DE DE69012579T patent/DE69012579T2/de not_active Expired - Fee Related
- 1990-07-30 WO PCT/US1990/004237 patent/WO1991003486A1/en active IP Right Grant
- 1990-07-30 AT AT90912171T patent/ATE111480T1/de active
- 1990-08-24 NZ NZ235040A patent/NZ235040A/en unknown
Also Published As
Publication number | Publication date |
---|---|
DE69012579D1 (de) | 1994-10-20 |
EP0494873A1 (de) | 1992-07-22 |
DE69012579T2 (de) | 1995-02-23 |
AU6171590A (en) | 1991-04-08 |
EP0494873B1 (de) | 1994-09-14 |
AU643456B2 (en) | 1993-11-18 |
US5047511A (en) | 1991-09-10 |
WO1991003486A1 (en) | 1991-03-21 |
NZ235040A (en) | 1992-03-26 |
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