ATE73138T1 - Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern. - Google Patents

Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern.

Info

Publication number
ATE73138T1
ATE73138T1 AT86306871T AT86306871T ATE73138T1 AT E73138 T1 ATE73138 T1 AT E73138T1 AT 86306871 T AT86306871 T AT 86306871T AT 86306871 T AT86306871 T AT 86306871T AT E73138 T1 ATE73138 T1 AT E73138T1
Authority
AT
Austria
Prior art keywords
inclusion bodies
purification
activation
proteins
sds
Prior art date
Application number
AT86306871T
Other languages
English (en)
Inventor
Steven K Rausch
Hsi Meng
Original Assignee
Int Minerals & Chem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Minerals & Chem Corp filed Critical Int Minerals & Chem Corp
Application granted granted Critical
Publication of ATE73138T1 publication Critical patent/ATE73138T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT86306871T 1985-09-06 1986-09-05 Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern. ATE73138T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/773,174 US4677196A (en) 1985-09-06 1985-09-06 Purification and activation of proteins from insoluble inclusion bodies
EP86306871A EP0218374B1 (de) 1985-09-06 1986-09-05 Reinigung und Aktivierung von Proteinen aus unlöslichen Einschlusskörpern

Publications (1)

Publication Number Publication Date
ATE73138T1 true ATE73138T1 (de) 1992-03-15

Family

ID=25097436

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86306871T ATE73138T1 (de) 1985-09-06 1986-09-05 Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern.

Country Status (9)

Country Link
US (1) US4677196A (de)
EP (1) EP0218374B1 (de)
JP (1) JPH082313B2 (de)
AT (1) ATE73138T1 (de)
CA (1) CA1267496C (de)
DE (1) DE3684078D1 (de)
DK (1) DK167192B1 (de)
IE (1) IE60644B1 (de)
IL (1) IL79957A (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5792450A (en) * 1985-02-05 1998-08-11 Chiron Corporation Purified human CSF-1
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) * 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
CA1305285C (en) * 1987-04-21 1992-07-14 Malcolm Roy Brandon Production of proteins in active forms
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US4801691A (en) * 1987-05-15 1989-01-31 International Minerals & Chemical Corp. Method for removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilized protein solutions
AU609824B2 (en) * 1987-06-15 1991-05-09 Southern Cross Biotech Pty Ltd. Production of proteins in active forms
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
US5094960A (en) * 1988-10-07 1992-03-10 New York Blood Center, Inc. Removal of process chemicals from labile biological mixtures by hydrophobic interaction chromatography
ES2064412T3 (es) * 1988-10-26 1995-02-01 American Cyanamid Co Procedimiento para reducir el contenido de aglomerado de materiales tipo hormona del crecimiento.
GB8825183D0 (en) * 1988-10-27 1988-11-30 Atomic Energy Authority Uk Recovery of substances
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
US5047511A (en) * 1989-08-28 1991-09-10 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5204256A (en) * 1989-09-06 1993-04-20 Behringwerke Aktiengesellschaft Process for the purification of plasminogen activator inhibitor 2 (pai-2)
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
US5605691A (en) * 1992-09-17 1997-02-25 Ophidian Pharmaceuticals, Inc. Immunologically active proteins from inclusion bodies
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5861372A (en) * 1996-02-22 1999-01-19 The Children's Medical Center Corporation Aggregate angiostatin and method of use
JPH11509551A (ja) * 1995-07-21 1999-08-24 アムジエン・インコーポレーテツド 蛋白質を塩から分離することのできる連続静止相を用いてバッファー交換により蛋白質を巻き戻す方法
US6346510B1 (en) 1995-10-23 2002-02-12 The Children's Medical Center Corporation Therapeutic antiangiogenic endostatin compositions
KR100392780B1 (ko) * 1996-12-04 2003-11-01 주식회사 엘지생명과학 대장균에서 발현된 세포외 신호조절 단백질카이네이즈-2의 정제방법
ZA9711733B (en) * 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
WO1998030684A1 (en) 1997-01-08 1998-07-16 Life Technologies, Inc. Methods for production of proteins
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
DE69934585T2 (de) * 1998-07-09 2007-10-25 BaroFold, Inc., Boulder Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
CA2408851C (en) 2000-05-16 2011-07-12 Bolder Biotechnology, Inc. Methods for refolding proteins containing free cysteine residues
MXPA03003867A (es) * 2000-10-31 2004-10-15 Univ Colorado Regents Desagregacion mejorada de proteina y replegamiento utilizando alta presion.
US6936437B2 (en) 2001-02-23 2005-08-30 Lucia Irene Gonzalez-Villasenor Methods and compositions for production of recombinant peptides
US20070036258A1 (en) * 2003-09-30 2007-02-15 Osamu Ito Process for producing radioactive fluorine compound
JP2009544974A (ja) 2006-07-21 2009-12-17 ライフ テクノロジーズ コーポレーション 鮮明に分解する標識タンパク質分子量標準
EP2059523A2 (de) * 2006-09-15 2009-05-20 Barofold, Inc. Hochdruckbehandlung von proteinen für reduzierte immunogenizität
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
SG178870A1 (en) * 2009-08-26 2012-04-27 Commw Scient Ind Res Org Processes for producing silk dope
WO2017040363A1 (en) * 2015-09-02 2017-03-09 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4426323A (en) * 1980-01-10 1984-01-17 Ionics, Incorporated Selected recovery of proteins from fermentation broths
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins

Also Published As

Publication number Publication date
EP0218374B1 (de) 1992-03-04
DK167192B1 (da) 1993-09-13
US4677196A (en) 1987-06-30
CA1267496A (en) 1990-04-03
JPS6269998A (ja) 1987-03-31
IE60644B1 (en) 1994-08-10
DK426386D0 (da) 1986-09-05
EP0218374A1 (de) 1987-04-15
JPH082313B2 (ja) 1996-01-17
DK426386A (da) 1987-03-07
IE862370L (en) 1987-03-06
CA1267496C (en) 1990-04-03
IL79957A0 (en) 1986-12-31
IL79957A (en) 1991-08-16
DE3684078D1 (de) 1992-04-09

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee