KR870004056A - 단백질 아미노-종결잔기의 선택적인 화학적 제거방법 - Google Patents

단백질 아미노-종결잔기의 선택적인 화학적 제거방법 Download PDF

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KR870004056A
KR870004056A KR1019860008982A KR860008982A KR870004056A KR 870004056 A KR870004056 A KR 870004056A KR 1019860008982 A KR1019860008982 A KR 1019860008982A KR 860008982 A KR860008982 A KR 860008982A KR 870004056 A KR870004056 A KR 870004056A
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acid
peptide
reaction
carried out
growth hormone
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KR1019860008982A
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KR890004352B1 (ko
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데니스 디마시 리차드
스테펜 브루크 제랄드
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메리 앤 터커
일라이 릴리 앤드 캄파니
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

단백질 아미노-종결잔기의 선택적인 화학적 제거방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (19)

  1. 일반식 H-X-Pro-펩티드(여기에서,X는 천연생성 아미노산이고 ; 펩티드는 생물학적 활성 펩티드 또는 단백질을 정의하는 아미노산 서열이다)를 a) 비양성자성 용매중의 약산성 조건 또는 b) 완충수성매질 중의 산성·중성 또는 알칼리성 조건하에 두어 펩티드를 분해 및 분리시키는 동시에 H-X-Pro잔기의 디케토피페라진을 형성함을 특징으로 하여 생물학적 활성 펩티드 또는 단백질을 정의하는 아미노산 서열을 갖는 화합물을 제조하는 방법.
  2. 제 1 항에 있어서, 비양성자성 용매가 N-메틸프롤리디논, N,N-디메틸포름아미드, 디메틸설폭사디이드 포름아미드 또는 헥사메틸포스포르아미드인 방법.
  3. 제 2 항에 있어서, 비양성자성 용매가 N-메틸피롤리디논인 방법.
  4. 제 1 항, 제 2 항 또는 제 3 항에 있어서, 아세트산, 인산, 아인산, 황산, 트리플루오로아세트산, 포름산, 말레산, 타르타르산 또는 글리신을 가하여 약산성 조건을 유도하는 방법.
  5. 제 4 항에 있어서, 산이 아세트산인 방법.
  6. 제 1 항 내지 제 5 항중 어느 한 항에 있어서, 약 15 내지 약 50℃의 온도에서 반응을 수행하는 방법.
  7. 제 1 항 내지 제 6 항중 어느 한 항에 있어서, 산의 농도가 약 0.1 내지 약 1M인 방법.
  8. 제 7 항에 있어서, 약 0.33M의 아세트산을 사용하여 반응을 수행하는 방법.
  9. 제 1 항에 있어서, H-X-Pro-펩티드를 완충 수성 매질중의 중성 또는 알칼리성 조건하에 두는 방법.
  10. 제 9 항에 있어서, 완충염이 인산염, 아인산염 또는 아황산염인 방법.
  11. 제10항에 있어서, 완충염이 약 0.1 내지 약 2.0M의 농도로 존재하는 인산염인 방법.
  12. 제11항에 있어서, 인산염의 농도가 약 0.8 내지 약 1M인 방법.
  13. 제 1 항 또는 제 9 항 내지 제12항중 어느 한 항에 있어서, 약 7 내지 9의 pH에서 반응을 수행하는 방법.
  14. 제13항에 있어서, 약 8의 pH에서 반응을 수행하는 방법.
  15. 제 1 항 또는 제 9 항 내지 제13항중 어느 한 항에 있어서, 약 25 내지 약 60℃의 온도에서 반응을 수행하는 방법.
  16. 제 1 항 내지 제15항중 어느 한 항에 있어서, X가 Met인 방법.
  17. 제 1 항 내지 제16항중 어느 한 항에 있어서, 펩티드가 프로인슐린, 인체 성장호르몬, 소의 성장호르몬, α-인터페론, β-인터페론, γ-인터페론, 인터류킨-1, 인터류킨-2, 성장호르몬 방출인자, 인슐린 유사 성장인자, 조직 플라스미노겐 활성인자, 단백질 또는 상기한 것들 중 어느 하나의 개질된 생물학적 활성 형태를 정의하는 아미노산 잔기의 서열인 방법.
  18. 제17항에 있어서, 펩티드가 인체 성장 호르몬인 방법.
  19. 제17항에 있어서, 펩티드가 소의 성장 호르몬인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860008982A 1985-10-28 1986-10-27 단백질 아미노-종결잔기의 선택적인 화학적 제거방법 KR890004352B1 (ko)

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US79183785A 1985-10-28 1985-10-28
US719.837 1985-10-28
US791,837 1991-11-14

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KR870004056A true KR870004056A (ko) 1987-05-07
KR890004352B1 KR890004352B1 (ko) 1989-10-31

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EP (1) EP0220958A3 (ko)
JP (1) JPS62106100A (ko)
KR (1) KR890004352B1 (ko)
CN (1) CN86107569A (ko)
AU (1) AU6441486A (ko)
DK (1) DK512286A (ko)
HU (1) HUT41811A (ko)
IL (1) IL80422A0 (ko)
NZ (1) NZ218088A (ko)
PT (1) PT83613B (ko)
SU (1) SU1531858A3 (ko)
ZA (1) ZA868168B (ko)

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DK512286A (da) 1987-04-29
JPS62106100A (ja) 1987-05-16
ZA868168B (en) 1988-06-29
EP0220958A2 (en) 1987-05-06
EP0220958A3 (en) 1989-01-25
KR890004352B1 (ko) 1989-10-31
CN86107569A (zh) 1987-07-08
SU1531858A3 (ru) 1989-12-23
PT83613A (en) 1986-11-01
NZ218088A (en) 1989-01-27
PT83613B (en) 1988-11-21
HUT41811A (en) 1987-05-28
DK512286D0 (da) 1986-10-27
AU6441486A (en) 1987-04-30
IL80422A0 (en) 1987-01-30

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