DK2729572T3 - Alfa-amylasevarianter og polynukleotider, som koder for dem - Google Patents
Alfa-amylasevarianter og polynukleotider, som koder for dem Download PDFInfo
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- DK2729572T3 DK2729572T3 DK12738308.1T DK12738308T DK2729572T3 DK 2729572 T3 DK2729572 T3 DK 2729572T3 DK 12738308 T DK12738308 T DK 12738308T DK 2729572 T3 DK2729572 T3 DK 2729572T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Claims (4)
1. Alfa-amyiasevariant eller fragment deraf med forbedret termostabilitet, målt som resterende amylaseaktivitet efter inkubation I 1 time ved 65°C ved pH 3,5, i sammenligning med en stam-aifa-amylase, som er beskrevet som SEQ ID NO: 3, idet alfa-amyiasevarianten eller fragmentet deraf omfatter en substitution i en position svarende til position 143 og endvidere omfatter en eller flere substitutioner i positionersvarende til position 128, 141, 192, 20, 76, 123, 136, 142, 165, 219, 224, 265, 383 og 410 i polypeptidet ifølge SEQ ID NO: 3, hvor alfa-amylasevarianten eller fragmentet deraf udviser mindst 80%, mindst 85%, mindst 90%, mindst 95%, mindst 96%, mindst 97%, mindst 98% eller mindst 99% sekvensidentitet med polypeptidet ifølge SEQ ID NO: 3, og hvor aifa-amylasevarianten eller et fragment deraf omfatter mindst én af følgende substitutioner eller kombinationer af substitutioner: G128D + D143N; eller N142D + D143N; eller Y141W+ D143N; eller Y141W + N142D + D143N; eller G128D + Y141W + D143N; eller Y141W + D143N + P219C; eller Y141W + D143N + K192R; eller G128D + D143N + K192R; eller Y141W + D143N + K192R + P219C; eller G128D + Y141W + D143N + K192R; eller G128D + Y141W + D143N + K192R + P219C.
2, Variant ifølge krav 1, hvor varianten endvidere omfatter en linker og et carbohydratbindende modul (CBM).
3. Variant ifølge krav 2, hvor CBM'et er et polypeptid, som omfatter en aminosyresekvens, der udviser mindst 60% identitet med en sekvens valgt fra gruppen bestående af SEQ ID NO: 27, SEQ ID NO: 28, SEG ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43 og SEQ ID NO: 44.
4, Variant ifølge krav 3, hvor linkeren og CBM'et stammer fra Athelia rolfsii, f.eks. SEQ ID NQ: 19 og SEQ ID NO: 36 eller en sekvens, som udviser 60% identitet dermed.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US201161504771P | 2011-07-06 | 2011-07-06 | |
US201161505192P | 2011-07-07 | 2011-07-07 | |
PCT/US2012/045670 WO2013006756A2 (en) | 2011-07-06 | 2012-07-06 | Alpha amylase variants and polynucleotides encoding same |
Publications (1)
Publication Number | Publication Date |
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DK2729572T3 true DK2729572T3 (da) | 2019-04-23 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK12738308.1T DK2729572T3 (da) | 2011-07-06 | 2012-07-06 | Alfa-amylasevarianter og polynukleotider, som koder for dem |
Country Status (10)
Country | Link |
---|---|
US (2) | US9267124B2 (da) |
EP (1) | EP2729572B1 (da) |
CN (1) | CN103781910B (da) |
BR (1) | BR112014000143B1 (da) |
CA (1) | CA2840962C (da) |
DK (1) | DK2729572T3 (da) |
EA (1) | EA201490216A1 (da) |
ES (1) | ES2729385T3 (da) |
MX (1) | MX337984B (da) |
WO (1) | WO2013006756A2 (da) |
Families Citing this family (52)
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ES2935920T3 (es) | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
EP2859110B1 (en) | 2012-03-30 | 2022-10-26 | Novozymes North America, Inc. | Processes of producing fermentation products |
CN105208869A (zh) | 2013-04-05 | 2015-12-30 | 诺维信公司 | 用α-淀粉酶、脂肪酶和磷脂酶生产烘焙产品的方法 |
CA2915063C (en) | 2013-06-24 | 2023-08-15 | Novozymes A/S | Process of extracting oil from thin stillage |
US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
WO2015007639A1 (en) | 2013-07-17 | 2015-01-22 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
HUE056192T2 (hu) | 2014-01-22 | 2022-01-28 | Novozymes As | Pullulanázvariánsok és azokat kódoló polinukleotidok |
EP3209788B1 (en) | 2014-10-23 | 2019-06-05 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
US10308963B2 (en) | 2015-02-27 | 2019-06-04 | Novozymes A/S | Processes of producing ethanol using a fermenting organism |
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US20180208916A1 (en) | 2015-07-23 | 2018-07-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3394275A1 (en) | 2015-12-22 | 2018-10-31 | Novozymes A/S | Process of extracting oil from thin stillage |
US11104909B2 (en) | 2016-02-26 | 2021-08-31 | Nanjing Bestzyme Bio-Engineering Co., Ltd. | α-amylase variant and use thereof |
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BR112020024347A2 (pt) | 2018-05-31 | 2021-02-23 | Novozymes A/S | processos para aumentar o crescimento e a produtividade de levedura |
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BR112021017085A2 (pt) | 2019-04-02 | 2022-02-08 | Novozymes As | Processo de produção de um produto de fermentação |
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CN113122592A (zh) * | 2019-12-30 | 2021-07-16 | 中国科学院天津工业生物技术研究所 | 一种淀粉或其衍生物的酶法催化利用方法 |
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WO1999028448A1 (en) | 1997-11-26 | 1999-06-10 | Novo Nordisk A/S | Thermostable glucoamylase |
DK2316929T3 (da) | 1998-02-27 | 2016-07-25 | Novozymes As | Maltogene alfa-amylasevarianter |
WO2000004136A1 (en) | 1998-07-15 | 2000-01-27 | Novozymes A/S | Glucoamylase variants |
DE69932345T2 (de) | 1998-10-26 | 2007-07-19 | Novozymes A/S | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
WO2000056900A2 (en) | 1999-03-22 | 2000-09-28 | Novo Nordisk Biotech, Inc. | Promoter sequences derived from fusarium venenatum and uses thereof |
EP2290060B1 (en) | 1999-03-30 | 2016-12-07 | Novozymes A/S | Alpha-amylase variants |
EP1200566A2 (en) | 1999-07-09 | 2002-05-02 | Novozymes A/S | Glucoamylase variant |
CA2416967C (en) | 2000-08-01 | 2014-02-18 | Novozymes A/S | Alpha-amylase mutants with altered properties |
US7151204B2 (en) | 2001-01-09 | 2006-12-19 | Monsanto Technology Llc | Maize chloroplast aldolase promoter compositions and methods for use thereof |
CA2455013A1 (en) | 2001-07-27 | 2003-02-13 | Francesca Storici | Systems for in vivo site-directed mutagenesis using oligonucleotides |
DK1576152T3 (da) * | 2002-12-17 | 2007-04-10 | Novozymes As | Varmestabile alfa-amylaser |
DE602004024964D1 (de) | 2003-03-10 | 2010-02-25 | Novozymes As | Verfahren zur herstellung von alkohol |
DK2336308T3 (da) | 2003-06-25 | 2014-11-10 | Novozymes As | Enzymer til forarbejdning af stivelse |
WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
JP5452869B2 (ja) * | 2004-12-22 | 2014-03-26 | ノボザイムス アクティーゼルスカブ | デンプン加工法 |
WO2009030728A2 (en) | 2007-09-05 | 2009-03-12 | Novozymes A/S | Enzyme compositions with stabilizing constituent |
WO2013006756A2 (en) * | 2011-07-06 | 2013-01-10 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
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- 2012-07-06 MX MX2013014735A patent/MX337984B/es active IP Right Grant
- 2012-07-06 EA EA201490216A patent/EA201490216A1/ru unknown
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- 2012-07-06 CA CA2840962A patent/CA2840962C/en active Active
- 2012-07-06 US US14/131,148 patent/US9267124B2/en active Active
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BR112014000143A2 (pt) | 2017-02-07 |
US20160130572A1 (en) | 2016-05-12 |
US20140147893A1 (en) | 2014-05-29 |
WO2013006756A3 (en) | 2013-05-02 |
BR112014000143B1 (pt) | 2021-04-06 |
MX337984B (es) | 2016-03-30 |
CA2840962C (en) | 2021-04-13 |
US9765316B2 (en) | 2017-09-19 |
WO2013006756A2 (en) | 2013-01-10 |
EP2729572A2 (en) | 2014-05-14 |
MX2013014735A (es) | 2014-02-11 |
CN103781910B (zh) | 2019-04-23 |
US9267124B2 (en) | 2016-02-23 |
CN103781910A (zh) | 2014-05-07 |
ES2729385T3 (es) | 2019-11-04 |
EP2729572B1 (en) | 2019-03-20 |
CA2840962A1 (en) | 2013-01-10 |
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