DK2695943T3 - Expression system - Google Patents

Expression system Download PDF

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Publication number
DK2695943T3
DK2695943T3 DK13191424.4T DK13191424T DK2695943T3 DK 2695943 T3 DK2695943 T3 DK 2695943T3 DK 13191424 T DK13191424 T DK 13191424T DK 2695943 T3 DK2695943 T3 DK 2695943T3
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DK
Denmark
Prior art keywords
vector
expression
dna
expression system
oligonucleotide
Prior art date
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DK13191424.4T
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Danish (da)
English (en)
Inventor
Ian Hodgson
Christopher Lennon
Bhupendra Kara
Original Assignee
Fujifilm Diosynth Biotechnologies Uk Ltd
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Publication of DK2695943T3 publication Critical patent/DK2695943T3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK13191424.4T 2006-02-03 2007-02-01 Expression system DK2695943T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0602173.7A GB0602173D0 (en) 2006-02-03 2006-02-03 Expression system
EP11171994.4A EP2386642B1 (en) 2006-02-03 2007-02-01 Expression system

Publications (1)

Publication Number Publication Date
DK2695943T3 true DK2695943T3 (en) 2016-04-18

Family

ID=36100973

Family Applications (4)

Application Number Title Priority Date Filing Date
DK07712658.9T DK1984506T3 (da) 2006-02-03 2007-02-01 Ekspressionssystem
DK11172432.4T DK2386644T3 (en) 2006-02-03 2007-02-01 Expression system
DK13191424.4T DK2695943T3 (en) 2006-02-03 2007-02-01 Expression system
DK11171994.4T DK2386642T3 (en) 2006-02-03 2007-02-01 Expression system

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DK07712658.9T DK1984506T3 (da) 2006-02-03 2007-02-01 Ekspressionssystem
DK11172432.4T DK2386644T3 (en) 2006-02-03 2007-02-01 Expression system

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK11171994.4T DK2386642T3 (en) 2006-02-03 2007-02-01 Expression system

Country Status (15)

Country Link
US (4) US8530188B2 (pl)
EP (6) EP2695943B1 (pl)
JP (5) JP5221385B2 (pl)
KR (3) KR101624345B1 (pl)
CN (3) CN101410523B (pl)
CA (2) CA2959915C (pl)
DK (4) DK1984506T3 (pl)
ES (3) ES2436883T3 (pl)
GB (1) GB0602173D0 (pl)
HU (1) HUE027350T2 (pl)
PL (3) PL2695943T3 (pl)
PT (2) PT1984506E (pl)
SG (3) SG169389A1 (pl)
WO (1) WO2007088371A2 (pl)
ZA (2) ZA200806238B (pl)

Families Citing this family (19)

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GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
GB0709061D0 (en) 2007-05-11 2007-06-20 Avecia Biolog Ltd Expression system
GB0810154D0 (en) 2008-06-04 2008-07-09 Avecia Biolog Ltd Expression vector
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process
GB201018664D0 (en) 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
GB201209896D0 (en) 2012-06-01 2012-07-18 Fujifilm Diosynth Biotechnologies Uk Ltd Process
AU2014211438B2 (en) 2013-01-31 2017-02-02 Glaxo Group Limited Method of producing a protein
EP2992104B1 (en) * 2013-05-03 2019-04-17 Fujifilm Diosynth Biotechnologies UK Limited Expression process
GB201311837D0 (en) 2013-07-01 2013-08-14 Fujifilm Diosynth Biotechnologies Uk Ltd Expression Process
GB201319930D0 (en) 2013-11-12 2013-12-25 Fujifilm Diosynth Biotechnologies Uk Ltd Expression process
JP6803328B2 (ja) * 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
US9791697B2 (en) 2015-12-11 2017-10-17 Panasonic Intellectual Property Management Co., Ltd. Screen
MX2018011834A (es) 2016-03-29 2019-03-06 Geltor Inc Expresión de proteínas en bacterias gram negativas en la que la relación del volumen periplasmático con respecto al volumen citoplasmático está entre 0.5:1 y 10:1.
KR102504317B1 (ko) 2016-05-06 2023-02-24 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 재조합 단백질을 생성시키는 방법
GB201704659D0 (en) * 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
CA3174377A1 (en) * 2020-04-09 2021-10-14 Samuel Newton Sathyanesan Compositions comprising recombinant epo and methods of use thereof
US20230167467A1 (en) * 2020-04-15 2023-06-01 University of Alaska Anchorage Methods and compositions for the production of isobutene
AU2022413768A1 (en) 2021-12-17 2024-06-13 Fujifilm Diosynth Biotechnologies Uk Limited Sequences and methods for production of recombinant biological molecules in vesicles

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GB8517071D0 (en) * 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5059530A (en) * 1985-09-30 1991-10-22 Suntory Ltd. Expression vector for human TNF
JPS63267288A (ja) 1986-12-26 1988-11-04 Hoechst Japan Kk 増幅された遺伝子によつて蛋白質を生産する方法およびそのための遺伝子
US5510099A (en) * 1987-05-01 1996-04-23 Stratagene Mutagenesis testing using transgenic non-human animals carrying test DNA sequences
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
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JP4854202B2 (ja) * 2004-03-31 2012-01-18 株式会社日本触媒 新規改変型ｓ−ヒドロキシニトリルリアーゼ
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JP2005341803A (ja) * 2004-05-31 2005-12-15 Institute Of Physical & Chemical Research 非対称な塩基配列を認識して切断する酵素Ｉ−ＣｓｍＩ及びその製造方法
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
RU2006129690A (ru) 2006-08-16 2008-02-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ
WO2008051854A2 (en) 2006-10-20 2008-05-02 Trustees Of Boston University A tunable genetic switch for regulating gene expression

Also Published As

Publication number Publication date
JP2015006193A (ja) 2015-01-15
CA2959915C (en) 2022-11-15
CA2959915A1 (en) 2007-08-09
CN102690834B (zh) 2018-09-04
DK2386642T3 (en) 2014-02-17
ES2436883T3 (es) 2014-01-07
CN108841848B (zh) 2022-03-29
ZA200806238B (en) 2010-10-27
PL2386642T3 (pl) 2014-04-30
KR20080098013A (ko) 2008-11-06
EP2390334A2 (en) 2011-11-30
US9677103B2 (en) 2017-06-13
KR101492844B1 (ko) 2015-02-16
EP2386642B1 (en) 2013-11-13
US20140065670A1 (en) 2014-03-06
EP2386643B1 (en) 2017-04-12
EP2386642A3 (en) 2012-06-13
DK1984506T3 (da) 2013-09-16
JP2009525038A (ja) 2009-07-09
WO2007088371A3 (en) 2007-10-04
PT2386642E (pt) 2014-02-12
EP2386643A2 (en) 2011-11-16
KR20140119202A (ko) 2014-10-08
JP5221385B2 (ja) 2013-06-26
CN101410523B (zh) 2012-05-16
JP6298481B2 (ja) 2018-03-20
PL2695943T3 (pl) 2016-07-29
KR20140090699A (ko) 2014-07-17
CN102690834A (zh) 2012-09-26
EP2386642A2 (en) 2011-11-16
US20210348205A1 (en) 2021-11-11
EP2386644A3 (en) 2012-11-28
CA2637818A1 (en) 2007-08-09
JP6571707B2 (ja) 2019-09-04
ZA201004904B (en) 2011-03-30
SG169389A1 (en) 2011-03-30
EP2386643A3 (en) 2012-11-28
EP2386644A2 (en) 2011-11-16
JP2013078323A (ja) 2013-05-02
EP2695943B1 (en) 2016-01-20
EP2695943A1 (en) 2014-02-12
ES2569075T3 (es) 2016-05-06
JP2017136072A (ja) 2017-08-10
EP2390334A3 (en) 2012-11-28
US20090170160A1 (en) 2009-07-02
GB0602173D0 (en) 2006-03-15
EP2386644B1 (en) 2015-12-16
CN101410523A (zh) 2009-04-15
PT1984506E (pt) 2013-09-06
JP2016136959A (ja) 2016-08-04
ES2445173T3 (es) 2014-02-28
SG169390A1 (en) 2011-03-30
US20170240943A1 (en) 2017-08-24
SG169388A1 (en) 2011-03-30
PL1984506T3 (pl) 2013-11-29
HUE027350T2 (hu) 2016-09-28
EP1984506A2 (en) 2008-10-29
EP2390334B1 (en) 2016-08-10
US11098335B2 (en) 2021-08-24
KR101624345B1 (ko) 2016-06-07
KR101512776B1 (ko) 2015-04-16
DK2386644T3 (en) 2016-03-14
EP1984506B1 (en) 2013-06-05
JP5713984B2 (ja) 2015-05-07
WO2007088371A2 (en) 2007-08-09
CA2637818C (en) 2017-05-02
US8530188B2 (en) 2013-09-10
CN108841848A (zh) 2018-11-20

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