DK2635594T3 - Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, der koder for dem Download PDF

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Publication number
DK2635594T3
DK2635594T3 DK11837579.9T DK11837579T DK2635594T3 DK 2635594 T3 DK2635594 T3 DK 2635594T3 DK 11837579 T DK11837579 T DK 11837579T DK 2635594 T3 DK2635594 T3 DK 2635594T3
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polypeptide
seq
polynucleotide
acid
cellulosic material
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DK11837579.9T
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English (en)
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Ye Liu
Lan Tang
Junxin Duan
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Isoleret polypeptid med cellobiohydrolaseaktivitet, som er valgt fra gruppen bestående af: (a) et polypeptid, som udviser mindst 95%, f.eks. mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med aminosyrerne 19 til 469 ifølge SEQ ID NO: 2; (b) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 95%, f.eks. mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med nukleotiderne 55 til 1749 ifølge SEQ ID NO: 1; (c) en variant, som omfatter en substitution, deletion og/eller insertion af op til 10 aminosyrer i aminosyrerne 19 til 469 ifølge SEQ ID NO: 2; og (d) et fragment af et polypeptid, som udviser mindst 95% sekvensidentitet med aminosyrerne 19 til 469 ifølge SEQ ID NO: 2, et polypeptid, der kodes for af et polynukleotid, som udviser mindst 95% sekvensidentitet med nukleotiderne 55 til 1749 ifølge SEQ ID NO: 1, eller en variant, som omfatter en substitution, deletion og/eller insertion af op til 10 aminosyrer i aminosyrerne 19 til 469 ifølge SEQ ID NO: 2, hvor fragmentet har cellobiohydrolaseaktivitet, og hvor fragmentet indeholder mindst 390 aminosyrerester.
2. Polypeptid ifølge krav 1, som omfatter eller består af SEQ ID NO: 2 eller aminosyrerne 19 til 469 ifølge SEQ ID NO: 2.
3. Isoleret polypeptid, som omfatter et katalytisk domæne valgt fra gruppen bestående af: (a) et katalytisk domæne, der kodes for af et polynukleotid, som udviser mindst 96%, f.eks. mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med nukleotiderne 430 til 1749 ifølge SEQ ID NO: 1 eller cDNA-sekvensen deraf; og (b) en variant af aminosyrerne 112 til 469 ifølge SEQ ID NO: 2, som omfatter en substitution, deletion og/eller insertion af op til 10 aminosyrer.
4. Polypeptid ifølge et hvilket som helst af kravene 1-3, der kodes for af polynukleotidet, som er indeholdt i plasmid pGEM-T-CBHII46949-2, der er indeholdt i E. coli DSM 24143.
5. Polypeptid ifølge krav 3, som endvidere omfatter et cellulosebindende domæne.
6. Isoleret polynukleotid, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-5.
7. Rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 6, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet.
8. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-5, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform fremstiller polypeptidet, under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
9. Fremgangsmåde til fremstilling af et polypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af den rekombinante værtscelle ifølge krav 7 under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
10. Transgen plante, plantedel eller plantecelle, som er transformeret med et polynukleotid, hvilket polynukleotid koder for polypeptidet ifølge et hvilket som helst af kravene 1-5.
11. Fremgangsmåde til fremstilling af et polypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af den transgene plante eller plantecelle ifølge krav 10 under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
12. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med cellobiohydrolaseaktivitet ifølge et hvilket som helst af kravene 1-5.
13. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: _ o _ (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge et hvilket som helst af kravene 1-5; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
14. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge et hvilket som helst af kravene 1-5.
15. Totalmedieformulering eller celledyrkningssammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-5.
DK11837579.9T 2010-11-04 2011-11-02 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, der koder for dem DK2635594T3 (da)

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CN2010078419 2010-11-04
PCT/CN2011/081692 WO2012059053A1 (en) 2010-11-04 2011-11-02 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

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US (2) US9212354B2 (da)
EP (1) EP2635594B1 (da)
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US8778640B1 (en) * 2013-02-12 2014-07-15 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8778641B1 (en) * 2013-02-12 2014-07-15 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8993275B2 (en) * 2013-02-12 2015-03-31 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
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