DK1969123T3 - Hidtil ukendte enzymer - Google Patents

Hidtil ukendte enzymer Download PDF

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Publication number
DK1969123T3
DK1969123T3 DK06830937.6T DK06830937T DK1969123T3 DK 1969123 T3 DK1969123 T3 DK 1969123T3 DK 06830937 T DK06830937 T DK 06830937T DK 1969123 T3 DK1969123 T3 DK 1969123T3
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Denmark
Prior art keywords
gly
ser
ala
thr
pro
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DK06830937.6T
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English (en)
Inventor
Matti Siika-Aho
Liisa Viikari
Pentti Ojapalo
Leena Valtakari
Marika Alapuranen
Satu Jämsä
Jarno Kallio
Jari Vehmaanperä
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Ab Enzymes Oy
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38188307&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1969123(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from FI20055692A external-priority patent/FI119325B/fi
Priority claimed from US11/316,397 external-priority patent/US7256032B2/en
Application filed by Ab Enzymes Oy filed Critical Ab Enzymes Oy
Application granted granted Critical
Publication of DK1969123T3 publication Critical patent/DK1969123T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
    • D06P5/02—After-treatment
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
    • D21H21/06—Paper forming aids
    • D21H21/10—Retention agents or drainage improvers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Textile Engineering (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (22)

1. Endoglucanase-polypeptid, der omfatter cellulolytisk aktivitet og er valgt fra gruppen bestående af: a) et polypeptid omfattende en aminosyresekvens, der har mindst 95 % identitet med SEQ ID NO: 2, og b) et polypeptid af a), der mangler det kulhydrat-bindende domæne, eller det kulhydrat-bindende domæne og linker-regionen.
2. Endoglucanase-polypeptidet ifølge krav 1, hvor aminosyresekvensen har mindst 98 % sekvensidentitet med SEQ ID NO: 2.
3. Endoglucanase-polypeptidet ifølge krav 1, der har aminosyresekvensen af SEQ ID NO: 17 eller SEQ ID NO: 19.
4. Endoglucanase-polypeptidet ifølge krav 1, der er opnåeligt fra eller stammer fra Acremonium sp., fortrinsvis fra Acremonium thermophilum, mest fortrinsvis fra Acremonium sp. CBS 115240.
5. Endoglucanase-fusionsprotein omfattende den cellulolytisk aktive kerne af et polypeptid, der har mindst 95 % sekvensidentitet med SEQ ID NO 2, forbundet til et heterologt kulhydrat-bindende domæne (CBD).
6. Endoglucanase-fusionsproteinet ifølge krav 5, hvor CBD og en eventuel linker-region er afledt fra Trichoderma reesei CBHI, Chaetomium thermophilum CBHI, eller Acremonium sp. xylanase.
7. Endoglucanase-fusionsproteinet ifølge krav 6, hvor fusionsproteinet har en aminosyresekvens valgt fra gruppen bestående af en aminosyresekvens, der har mindst 71 % sekvensidentitet med SEQ ID NO: 39, mindst 69 % sekvensidentitet med SEQ ID NO: 40, og mindst 69 % sekvensidentitet med SEQ ID NO: 41.
8. Isoleret polynukleotid, der koder for endoglucanase-polypeptidet ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7.
9. Det isolerede polynukleotid ifølge krav 8, der har en nukleotidsekvens valgt fra gruppen bestående af: a) en nukleotidsekvens af SEQ ID NO: 1, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 37, eller SEQ ID NO: 38; b) en komplementær streng af a); og c) en sekvens, der er degenereret som et resultat af den genetiske kode til en hvilken som helst af sekvenserne som defineret i a) eller b).
10. Ekspressionsvektor omfattende en polynukleotidsekvens, der koder for endoglucanase-polypeptidet ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7.
11. Værtscelle omfattende en ekspressionsvektor ifølge krav 10.
12. Værtscellen ifølge krav 11, hvilken er af svampeoprindelse.
13. Værtscellen ifølge krav 12, hvilken er Trichoderma reesei.
14. Proces til fremstillingen af et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 omfattende trinnet at dyrke værtscellen ifølge et hvilket som helst af kravene 11 til 13.
15. Enzympræparat omfattende et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7.
16. Proces til biostoning, hvilken omfatter trinnet at tilsætte et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 eller et præparat ifølge krav 15 til bomuldsindeholdende stof eller klæder, såsom denim.
17. Proces til biofinishing, hvilken omfatter trinnet at tilsætte et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 eller et præparat ifølge krav 15 til tekstilmaterialer såsom stoffer eller klæder eller garn.
18. Detergentsammensætning omfattende et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 eller et præparat ifølge krav 15 og hjælpestoffer, såsom overfladeaktive stoffer, surfaktanter, blegemidler eller builder.
19. Fremgangsmåde til behandling af cellulosefiber-indeholdende tekstilmateriale, hvor fremgangsmåden omfatter at bringe tekstilmaterialet i kontakt med detergentsammensætningen ifølge krav 18.
20. Fremgangsmåde til behandling af træ-afledt pulp eller fiber, hvilken omfatter trinnet at tilsætte et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 eller et præparat ifølge krav 15 til træ-afledt mekanisk eller kemisk pulp eller sekundære fibre.
21. Fremgangsmåde til forbedring af kvaliteten af dyrefoder, hvilken omfatter at behandle plantemateriale med et endoglucanase-polypeptid ifølge et hvilket som helst af kravene 1 til 4 eller et endoglucanase-fusionsprotein ifølge et hvilket som helst af kravene 5 til 7 eller et præparat ifølge krav 15.
22. Escherichia coli-stamme med accessionsnummer DSM 17324, DSM 18813, DSM 18814, DSM 18815, DSM 18816 eller DSM 18817.
DK06830937.6T 2005-12-22 2006-12-15 Hidtil ukendte enzymer DK1969123T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20055692A FI119325B (fi) 2005-12-22 2005-12-22 Uusia endoglukanaasi polypeptidejä ja niiden tuotto ja käyttö
US11/316,397 US7256032B2 (en) 2005-12-22 2005-12-22 Enzymes
PCT/FI2006/050560 WO2007071820A1 (en) 2005-12-22 2006-12-15 Novel enzymes

Publications (1)

Publication Number Publication Date
DK1969123T3 true DK1969123T3 (da) 2017-08-28

Family

ID=38188307

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06830937.6T DK1969123T3 (da) 2005-12-22 2006-12-15 Hidtil ukendte enzymer

Country Status (7)

Country Link
EP (1) EP1969123B1 (da)
BR (1) BRPI0620318B1 (da)
DK (1) DK1969123T3 (da)
ES (1) ES2637008T3 (da)
MX (1) MX2008008225A (da)
PE (2) PE20071180A1 (da)
WO (1) WO2007071820A1 (da)

Families Citing this family (126)

* Cited by examiner, † Cited by third party
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JP3360830B2 (ja) 1995-03-17 2003-01-07 ノボザイムス アクティーゼルスカブ 新規なエンドグルカナーゼ
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