DK2365064T3 - Phytasevarianter - Google Patents
Phytasevarianter Download PDFInfo
- Publication number
- DK2365064T3 DK2365064T3 DK11155829T DK11155829T DK2365064T3 DK 2365064 T3 DK2365064 T3 DK 2365064T3 DK 11155829 T DK11155829 T DK 11155829T DK 11155829 T DK11155829 T DK 11155829T DK 2365064 T3 DK2365064 T3 DK 2365064T3
- Authority
- DK
- Denmark
- Prior art keywords
- phytase
- leu
- thr
- seq
- lys
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Detergent Compositions (AREA)
Claims (20)
1. Phytase, der har mindst 85 % identitet med SEQ ID NO: 2 og har en forbedret termostabilitet angivet som residualaktivitet, der er bestemt ved opdeling af en fermenteringssupernatant i to dele, hvor den ene del inkuberes i 30 minutter ved 60 °C og den anden del i 30 minutter ved 5 °C, hvorefter aktiviteten i begge bestemmes på p-nitrophenylphosphat ved 37 °C og pH 5,5, hvor phytasens residualaktivitet er aktiviteten i prøven, der er blevet inkuberet ved 60 °C, divideret med aktiviteten i den samme prøve, der er blevet inkuberet ved 5°C, hvor phytasens residualaktivitet er mindst 120% af residualaktiviteten af referencephytasen SEQ ID NO: 2 målt under de samme betingelser, og som omfatter mindst én ændring sammenlignet med SEQ ID NO: 2 i mindst én position, der er udvalgt blandt følgende: G52C/A99C, K141C/V199C, G59C/F100C, Q91C/W46C, N31C/E176C, N31C/T177C og/eller S162C/S247C, hvor graden af identitet mellem sekvenser bestemmes ved hjælp af programmet "Needle" ved anvendelse af substitutionsmatrixen BLOSUM62, idet gap-åbningsstraffen er 10, og gap-forlængelsesstraffen er 0,5.
2. Phytase ifølge krav 1, hvor aminosyrerne i position 179, 180, 181, 182, 183, 184, 185 og 186 er blevet erstattet med QADKP, GEDKP, NGISA, IAGKS, KEKHQ, KEKQQ, KEKKV eller KTDKL.
3. Phytase ifølge et hvilket som helst af kravene 1-2, der omfatter en ændring, der er udvalgt blandt følgende: 4P, 5P, 111P, 137P, 161P, 52E, 57Y, 76G, 107D, 107G, 109A, 1*, 172*, 17273*, 121T, 273L, 285G, 286Q, 299L, 362K, 331K/55D, 107E, 46E, 82E, 119R, 119K, 164E, 223E, 276R, 276K, 362R, 379R, 379K, 385D, 410D, 410E, 411 R, 411K, 53V, 121D, 167Q, 196Q, 200K, 202N, 218Q, 241Q, 285N, 314N, 314G, 406A, 179K/180E/181 K/182H/183Q/18471857186*, 179K/180E/181 K/182Q/183Q/18471857186*, 179 K/180E/181 K/182K/183V/18471857186*, 179 K/180T/181 D/182K/183L/18471857186*, 111 P/241Q, 1K, 114T/115Q/116A/117D/118T/119S/120S/121P/122D/123P/124L og 114T/115Q/116T/117D/118T/119S/120S/121 P/122D/123P/124L, hvor symbolet “*” betyder, at aminosyreresten i den angivne position er deleteret.
4. Phytase ifølge et hvilket som helst af kravene 1-3, der er en variant af phytasen ifølge SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6 eller SEQ ID NO: 9.
5. Phytase ifølge et hvilket som helst af kravene 1-4, der yderligere omfatter en substitution eller en kombination af substitutioner, der er udvalgt blandt substitutionerne og kombinationerne af substitutioner, der er anført i hver række i figur 1.
6. Isoleret nukleinsyre, der omfatter en nukleinsyresekvens, der koder for phytasen ifølge et hvilket som helst af kravene 1 -5.
7. Nukleinsyrekonstruktion, der omfatter nukleinsyresekvensen ifølge krav 6, der er operabelt koblet til en eller flere kontrolsekvenser, der styrer produktionen af phytasen i en egnet ekspressionsvært.
8. Rekombinant ekspressionsvektor, der omfatter nukleinsyrekonstruktionen ifølge krav 7.
9. Rekombinant værtscelle, der omfatter nukleinsyrekonstruktionen ifølge krav 7 og/eller ekspressionsvektoren ifølge krav 8.
10. Fremgangsmåde til produktion af phytasen ifølge et hvilket som helst af kravene 1-5, der omfatter (a) dyrkning af værtscellen ifølge krav 9 til frembringelse af en supernatant, der omfatter phytasen; og (b) indvinding af phytasen.
11. Transgen plante eller plantedel, der er blevet transformeret med en nukleinsyre ifølge krav 6 eller 7, der er i stand til at udtrykke en phytase ifølge et hvilket som helst af kravene 1 -5.
12. Transgent, ikke-humant dyr eller produkter eller elementer deraf, der er blevet transformeret med en nukleinsyre ifølge krav 6 eller 7, der er i stand til at udtrykke en phytase ifølge et hvilket som helst af kravene 1-5.
13. Sammensætning, der omfatter mindst én phytase ifølge et hvilket som helst af kravene 1-5 og (a) mindst ét fedtopløseligt vitamin; (b) mindst et vandopløseligt vitamin; og/eller (c) mindst ét spormineral.
14. Sammensætning ifølge krav 13, der yderligere omfatter mindst ét enzym, der er udvalgt fra følgende gruppe af enzymer: amylase, phytase, phosphatase, xylanase, galactanase, alfa-galactosidase, protease, phospholipase og/eller beta-glucanase.
15. Sammensætning ifølge et hvilket som helst af kravene 13-14, der er et tilsætningsstof til dyrefoder.
16. Dyrefodersammensætning, der har et råproteinindhold på 50 til 800 g/kg og omfatter phytasen ifølge et hvilket som helst af kravene 1-5 eller sammensætningen ifølge et hvilket som helst af kravene 13-15.
17. Fremgangsmåde til forbedring af ernæringsværdien af et dyrefoder, hvor phytasen ifølge et hvilket som helst af kravene 1-5 eller sammensætningen ifølge et hvilket som helst af kravene 13-15 tilsættes til foderet.
18. Fremgangsmåde til reducering af phytatniveauer i dyregødning, der omfatter fodring af et dyr med en effektiv mængde af foderet ifølge krav 16.
19. Fremgangsmåde til behandling af planteproteiner, der omfatter trinet tilsætning af phytasen ifølge et hvilket som helst af kravene 1-5 eller sammensætningen ifølge et hvilket som helst af kravene 13-15 til mindst ét planteprotein eller en planteproteinkilde.
20. Anvendelse af phytasen ifølge et hvilket som helst af kravene 1-5 eller sammensætningen ifølge et hvilket som helst af kravene 13-15 i dyrefoder; til fremstilling af dyrefoder; til forbedring af ernæringsværdien af dyrefoder; til reducering af phytatniveauer i dyregødning; til behandling af planteproteiner; eller til frigørelse af phosphor fra et phytasesubstrat.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200600484 | 2006-04-04 | ||
| DKPA200600581 | 2006-04-25 | ||
| EP07711278A EP2001999B1 (en) | 2006-04-04 | 2007-03-19 | Phytase variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2365064T3 true DK2365064T3 (da) | 2015-03-30 |
Family
ID=38123737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11155829T DK2365064T3 (da) | 2006-04-04 | 2007-03-19 | Phytasevarianter |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US8460656B2 (da) |
| EP (2) | EP2365064B1 (da) |
| JP (1) | JP5221516B2 (da) |
| AU (1) | AU2007234177B2 (da) |
| BR (1) | BRPI0709732B1 (da) |
| CA (1) | CA2647476C (da) |
| DK (1) | DK2365064T3 (da) |
| ES (2) | ES2531434T3 (da) |
| MX (1) | MX2008012632A (da) |
| PL (1) | PL2365064T3 (da) |
| WO (1) | WO2007112739A1 (da) |
Families Citing this family (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| ES2531434T3 (es) | 2006-04-04 | 2015-03-16 | Novozymes A/S | Variantes de fitasa |
| WO2008017066A2 (en) | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| EP2617814B1 (en) | 2006-09-21 | 2015-11-18 | BASF Enzymes LLC | Phytases, nucleic acids encoding them and methods for making and using them |
| ES2544645T3 (es) | 2008-09-26 | 2015-09-02 | Novozymes A/S | Variantes de fitasa de Hafnia |
| EP2408321A1 (en) | 2009-03-20 | 2012-01-25 | Novozymes A/S | A nutritional beverage and a method of making the same |
| AU2010249500B2 (en) | 2009-05-21 | 2016-03-24 | Basf Enzymes Llc | Phytases, nucleic acids encoding them and methods for making and using them |
| AU2013204082B2 (en) * | 2009-05-21 | 2016-03-31 | Basf Enzymes Llc | Phytases, nucleic acids encoding them and methods for making and using them |
| MX336638B (es) | 2009-10-22 | 2016-01-26 | Basf Se | Variantes sinteticas de fitasa. |
| RU2472855C2 (ru) * | 2009-12-15 | 2013-01-20 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) | МУТАНТНАЯ РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ФИТАЗА (ВАРИАНТЫ), ФРАГМЕНТ ДНК, КОДИРУЮЩИЙ УКАЗАННУЮ ФИТАЗУ (ВАРИАНТЫ), ШТАММ Pichia pastoris - ПРОДУЦЕНТ УКАЗАННОЙ ФИТАЗЫ (ВАРИАНТЫ) |
| EP2553091B1 (en) * | 2010-03-26 | 2019-05-08 | Novozymes A/S | Thermostable phytase variants |
| US20130195829A1 (en) * | 2010-03-26 | 2013-08-01 | Novozymes A/S | Themostable Phytase Variants |
| CA2794113A1 (en) * | 2010-03-26 | 2011-09-29 | Novozymes A/S | Thermostable phytase variants |
| GB201102865D0 (en) | 2011-02-18 | 2011-04-06 | Danisco | Feed additive composition |
| WO2012110776A2 (en) * | 2011-02-18 | 2012-08-23 | Dupont Nutrition Biosciences Aps | Feed additive composition |
| GB201102857D0 (en) | 2011-02-18 | 2011-04-06 | Danisco | Feed additive composition |
| CN103502443B (zh) | 2011-04-21 | 2016-05-04 | 巴斯夫欧洲公司 | 合成的植酸酶变体 |
| KR101692103B1 (ko) | 2011-04-21 | 2017-01-02 | 바스프 에스이 | 합성 피타아제 변이체 |
| ES2705533T3 (es) | 2011-07-07 | 2019-03-25 | Dupont Nutrition Biosci Aps | Ensayo |
| GB201213801D0 (en) | 2012-08-03 | 2012-09-12 | Dupont Nutrition Biosci Aps | Feed additive composition |
| DK3527667T3 (da) * | 2013-03-08 | 2021-02-22 | Biogrammatics Inc | Gærpromotorer til proteinekspression |
| PL2964765T3 (pl) | 2013-03-08 | 2019-10-31 | Keck Graduate Inst Of Applied Life Sciences | Promotory drożdżowe z pichia pastoris |
| EP3712274A1 (en) | 2013-09-11 | 2020-09-23 | Novozymes A/S | Processes for producing fermentation products |
| MX2016016871A (es) | 2014-06-27 | 2017-04-25 | Dsm Ip Assets Bv | Metodo para mejorar el valor nutricional de pienso para animales. |
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| EP3394274A1 (en) | 2015-12-22 | 2018-10-31 | Novozymes A/S | Processes for producing fermentation products |
| TWI615471B (zh) * | 2016-02-18 | 2018-02-21 | 基酵生物科技股份有限公司 | 提升耐熱性的植酸酶 |
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| US20200359656A1 (en) | 2018-02-08 | 2020-11-19 | Danisco Us Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
| CN112367847A (zh) | 2018-04-26 | 2021-02-12 | 丹尼斯科美国公司 | 增加固体组合物中植酸酶的稳定性的方法及包含磷酸盐和植酸酶的颗粒组合物 |
| MX2020012754A (es) | 2018-05-31 | 2021-02-26 | Novozymes As | Procesos para mejorar el crecimiento y la productividad de la levadura. |
| RU2706086C1 (ru) * | 2018-10-29 | 2019-11-13 | Акционерное Общество "Биоамид" | Мутантная рекомбинантная термостабильная фитаза |
| CA3120360A1 (en) | 2018-11-20 | 2020-05-28 | Dupont Nutrition Biosciences Aps | Engineered robust high tm-phytase clade polypeptides and fragments thereof |
| BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
| EP3996519A1 (en) | 2019-07-09 | 2022-05-18 | DuPont Nutrition Biosciences ApS | Fat coated particulate enzyme compositions |
| BR112022002203A2 (pt) | 2019-08-05 | 2022-09-06 | Novozymes As | Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa |
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| US20220395543A1 (en) | 2019-10-21 | 2022-12-15 | Dupont Nutrition Biosciences Aps | Compositions for gut health |
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| CN115867146A (zh) | 2020-02-07 | 2023-03-28 | 杜邦营养生物科学有限公司 | 用于动物健康的饲料组合物 |
| TWI725754B (zh) | 2020-02-27 | 2021-04-21 | 金全益股份有限公司 | Ｒｇｖ進料系統 |
| BR112022017034A2 (pt) | 2020-02-28 | 2022-12-13 | Dupont Nutrition Biosci Aps | Composições de ração |
| EP4196575A1 (en) | 2020-08-13 | 2023-06-21 | Novozymes A/S | Phytase variants and polynucleotides encoding same |
| CN117241678A (zh) | 2020-10-16 | 2023-12-15 | 杜邦营养生物科学有限公司 | 用于动物健康的饲料组合物 |
| WO2022169933A2 (en) | 2021-02-03 | 2022-08-11 | Dupont Nutrition Biosciences Aps | Compositions for gut health |
| KR20240060703A (ko) | 2021-09-27 | 2024-05-08 | 인터내셔널 엔＆에이치 덴마크 에이피에스 | 사료 첨가제 조성물 및 이의 사용 방법 |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
| WO2024137246A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
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| PT1448595E (pt) | 2001-11-20 | 2007-02-28 | Novozymes As | Polipéptideos antimicrobianos de pseudoplectania nigrella |
| JP2005513042A (ja) | 2001-12-03 | 2005-05-12 | ノボザイムス アクティーゼルスカブ | スタチン様化合物 |
| AU2003238448A1 (en) * | 2002-05-30 | 2003-12-19 | Basf Aktiengesellschaft | Modified phytases |
| US7968342B2 (en) | 2002-08-12 | 2011-06-28 | Danisco Us Inc. | Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same |
| US7442398B2 (en) * | 2003-03-25 | 2008-10-28 | Republic Of National Fisheries Research And Development Institute | Phytase produced from Citrobacter braakii |
| JP2005137293A (ja) * | 2003-11-07 | 2005-06-02 | Mitsui Chemicals Inc | コドン改変と細胞外分泌の組合せにより外来フィターゼを植物で生産する方法 |
| GB0422052D0 (en) * | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| AR050895A1 (es) * | 2004-10-04 | 2006-11-29 | Novozymes As | Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican |
| AU2005290934C1 (en) | 2004-10-04 | 2013-05-09 | Dupont Nutrition Biosciences Aps | Citrobacter freundii phytase and homologues |
| ES2614744T3 (es) | 2004-10-04 | 2017-06-01 | Novozymes A/S | Polipéptidos con actividad de fitasa y polinucleótidos que codifican los mismos |
| JP2006136314A (ja) * | 2004-10-12 | 2006-06-01 | Mitsui Chemicals Inc | 新規改変フィターゼ |
| KR100663112B1 (ko) * | 2004-10-29 | 2007-01-02 | 주식회사 기성기전 | 절전형 전자개폐기 |
| WO2006063588A1 (en) * | 2004-12-13 | 2006-06-22 | Novozymes A/S | Polypeptides having acid phosphatase activity and polynucleotides encoding same |
| FR2888250B1 (fr) * | 2005-07-08 | 2007-08-17 | Adisseo France Sas Soc Par Act | Phytase de debaryomyces castellii |
| ES2531434T3 (es) | 2006-04-04 | 2015-03-16 | Novozymes A/S | Variantes de fitasa |
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2007
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- 2007-03-19 AU AU2007234177A patent/AU2007234177B2/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| ES2387203T3 (es) | 2012-09-17 |
| AU2007234177A1 (en) | 2007-10-11 |
| PL2365064T3 (pl) | 2015-05-29 |
| US20110258714A1 (en) | 2011-10-20 |
| US8460656B2 (en) | 2013-06-11 |
| BRPI0709732B1 (pt) | 2017-06-06 |
| JP2009532043A (ja) | 2009-09-10 |
| US20160362666A1 (en) | 2016-12-15 |
| EP2001999B1 (en) | 2012-05-16 |
| CA2647476C (en) | 2016-08-16 |
| US9451783B2 (en) | 2016-09-27 |
| CA2647476A1 (en) | 2007-10-11 |
| AU2007234177B2 (en) | 2012-11-08 |
| EP2365064A1 (en) | 2011-09-14 |
| US20130224332A1 (en) | 2013-08-29 |
| JP5221516B2 (ja) | 2013-06-26 |
| EP2001999A1 (en) | 2008-12-17 |
| US10041052B2 (en) | 2018-08-07 |
| EP2365064B1 (en) | 2014-12-24 |
| ES2531434T3 (es) | 2015-03-16 |
| US8877471B2 (en) | 2014-11-04 |
| WO2007112739A1 (en) | 2007-10-11 |
| BRPI0709732A2 (pt) | 2011-07-26 |
| US20150024084A1 (en) | 2015-01-22 |
| MX2008012632A (es) | 2008-10-13 |
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