DK2270140T3 - Lipolytiske enzymvarianter - Google Patents

Lipolytiske enzymvarianter Download PDF

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Publication number
DK2270140T3
DK2270140T3 DK10178844.6T DK10178844T DK2270140T3 DK 2270140 T3 DK2270140 T3 DK 2270140T3 DK 10178844 T DK10178844 T DK 10178844T DK 2270140 T3 DK2270140 T3 DK 2270140T3
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DK
Denmark
Prior art keywords
ala
gly
thr
ser
val
Prior art date
Application number
DK10178844.6T
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English (en)
Inventor
Kim Borch
Louise Erlandsen
Jesper Vind
Allan Svendsen
Christel Thea Joergensen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2270140T3 publication Critical patent/DK2270140T3/da

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Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01032—Phospholipase A1 (3.1.1.32)

Claims (6)

1. Polypeptid, som: består af SEQ ID NO: 2 med substitutionen P256V.
2. Polynukleotid, der koder for polypeptidet ifølge krav 1.
3. Fremgangsmåde til fremstilling af en dej eller et spiseligt produkt fremstillet af dej, hvilken fremgangsmåde omfatter tilsætning af polypeptidet ifølge krav 1 til dejen.
4. Fremgangsmåde ifølge krav 3, som endvidere omfatter en antiældningsamylase, navnlig en maltogen alfa-amylase.
5. Fremgangsmåde ifølge krav 4, som endvidere omfatter et yderligere enzym valgt blandt en amyloglucosidase, en beta-amylase, en pentosanase, en proteinase og/eller en glucoseoxidase.
6. Anvendelse af polypeptidet ifølge krav 1 til at reducere dejklæbrighed.
DK10178844.6T 2003-05-09 2004-04-29 Lipolytiske enzymvarianter DK2270140T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200300709 2003-05-09
DKPA200300811 2003-05-30
US47964703P 2003-06-19 2003-06-19
EP04730201A EP1625216B1 (en) 2003-05-09 2004-04-29 Variant lipolytic enzymes

Publications (1)

Publication Number Publication Date
DK2270140T3 true DK2270140T3 (da) 2016-03-14

Family

ID=43500457

Family Applications (3)

Application Number Title Priority Date Filing Date
DK10178844.6T DK2270140T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter
DK04730201.3T DK1625216T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter
DK10178829.7T DK2270139T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK04730201.3T DK1625216T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter
DK10178829.7T DK2270139T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter

Country Status (6)

Country Link
US (3) US7465570B2 (da)
EP (4) EP2290057A3 (da)
AU (2) AU2004236305B2 (da)
CA (1) CA2523400C (da)
DK (3) DK2270140T3 (da)
WO (1) WO2004099400A2 (da)

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EP2691532A1 (en) 2011-03-30 2014-02-05 Novozymes A/S Esterification process
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CN107475235B (zh) 2011-06-20 2022-09-13 诺维信公司 颗粒组合物
BR112014027861A2 (pt) 2012-05-11 2017-12-12 Novozymes As método de preparação de um mosto
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CN104718289A (zh) 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
MX363261B (es) * 2013-03-21 2019-03-19 Novozymes As Polipéptidos que tienen actividad de la fosfolipasa a y polinucleótidos que los codifican.
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MX2017000044A (es) * 2014-07-07 2017-05-01 Novozymes As Masa con enzima lipolitica y/o xilanasa y monooxigenasa.
WO2016030480A1 (en) * 2014-08-28 2016-03-03 Renescience A/S Solubilization of msw with blend enzymes
CN113913475A (zh) 2015-10-09 2022-01-11 诺维信公司 酶或非酶生物柴油精制方法
DE102015224576A1 (de) * 2015-12-08 2017-06-08 Henkel Ag & Co. Kgaa Lipasen mit erhöhter Thermostabilität
WO2018114941A1 (en) * 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) * 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
BR112019012559A2 (pt) * 2016-12-21 2019-11-26 Dsm Ip Assets Bv variantes de enzima lipolítica
WO2018114912A1 (en) * 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US11051520B2 (en) 2017-02-20 2021-07-06 Novozymes A/S Lipolytic enzyme for use in baking
US11744254B2 (en) * 2017-12-19 2023-09-05 Dupont Nutrition Biosciences Aps Enzymatic modification of phospholipids in food
CN108504643A (zh) * 2018-05-31 2018-09-07 华南理工大学 一种来源于米曲霉的偏甘油酯脂肪酶及其制备方法和晶体结构
EP3809852A1 (en) * 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
EP3937642A1 (en) 2019-03-13 2022-01-19 Novozymes A/S Production of par-baked products with improved freshness employing combination of gh8 xylanase and phospholipase
EP4156944A1 (en) 2020-05-29 2023-04-05 Novozymes A/S Pulse and/or legume protein-fortified doughs and baked goods comprising lipase
WO2022233897A1 (en) 2021-05-04 2022-11-10 Novozymes A/S Enzymatic treatment of feedstock for hydrotreated vegetable oil (hvo) production
WO2023203080A1 (en) 2022-04-20 2023-10-26 Novozymes A/S Process for producing free fatty acids
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
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WO2024079301A1 (en) 2022-10-14 2024-04-18 Novozymes A/S Process for selective hydrolysis of diglycerides in an oil/fat with aid of candida antarctica lipase b
WO2024088550A1 (en) 2022-10-24 2024-05-02 Novozymes A/S Baking method for pulse protein fortified bread employing thermostable amyloglucosidase variante (ec 3.2.1.3)

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Also Published As

Publication number Publication date
US7638317B2 (en) 2009-12-29
WO2004099400A2 (en) 2004-11-18
US8298800B2 (en) 2012-10-30
EP2270139A2 (en) 2011-01-05
EP2270140B1 (en) 2015-12-16
DK2270139T3 (da) 2016-11-07
EP2270139A3 (en) 2011-11-23
US7465570B2 (en) 2008-12-16
AU2004236305A1 (en) 2004-11-18
US20080311242A9 (en) 2008-12-18
EP2290057A2 (en) 2011-03-02
WO2004099400A3 (en) 2004-12-16
AU2010246333A1 (en) 2010-12-09
AU2010241524A1 (en) 2010-12-09
AU2010241524B2 (en) 2013-01-31
EP1625216A2 (en) 2006-02-15
EP2270139B1 (en) 2016-07-27
US20060228446A1 (en) 2006-10-12
US20100047388A1 (en) 2010-02-25
DK1625216T3 (da) 2013-03-18
EP2290057A3 (en) 2011-08-03
EP2270140A2 (en) 2011-01-05
AU2004236305B2 (en) 2010-08-19
EP2270140A3 (en) 2011-11-30
EP1625216B1 (en) 2012-11-21
CA2523400C (en) 2015-03-17
US20080293120A1 (en) 2008-11-27
CA2523400A1 (en) 2004-11-18

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