DK2270139T3 - Lipolytiske enzymvarianter - Google Patents

Lipolytiske enzymvarianter Download PDF

Info

Publication number
DK2270139T3
DK2270139T3 DK10178829.7T DK10178829T DK2270139T3 DK 2270139 T3 DK2270139 T3 DK 2270139T3 DK 10178829 T DK10178829 T DK 10178829T DK 2270139 T3 DK2270139 T3 DK 2270139T3
Authority
DK
Denmark
Prior art keywords
ala
gly
thr
ser
val
Prior art date
Application number
DK10178829.7T
Other languages
English (en)
Inventor
Kim Borch
Louise Erlandsen
Jesper Vind
Allan Svendsen
Christel Thea Joergensen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2270139T3 publication Critical patent/DK2270139T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01032—Phospholipase A1 (3.1.1.32)

Claims (7)

1. Polypeptid, som: a) har hydrolytisk aktivitet overfor en esterbinding i et polært lipid og b) har en aminosyresekvens, som udviser mindst 95% identitet med SEQ ID NO: 1, og hvor aminosyren i positionen svarende til A29 ifølge SEQ ID NO: 1 er P, og hvor polypeptidet fører til reduceret dejklæbrighed i sammenligning med polypeptidet, som består af sekvensen ifølge SEQ ID NO: 1, når de tilsættes til dejen.
2. Polypeptid ifølge krav 1, som har phospholipaseaktivitet eller galactolipaseaktivitet (EC 3.1.1.26), navnlig phospholipase A1-aktivitet (EC 3.1.1.32).
3. Polypeptid ifølge et hvilket som helst af kravene 1-2, hvor aminosyren i positionen svarende til K33 ifølge SEQ ID NO: 1 er N.
4. Polypeptid ifølge et hvilket som helst af kravene 1-3, hvor aminosyren i positionen svarende til I83 ifølge SEQ ID NO: 1 er N/C/W.
5. Polypeptid ifølge et hvilket som helst af kravene 1-2, som består af aminosyrerne 1-272, 1-273, 1-274 eller 1-286 ifølge SEQ ID NO: 1 med følgende substitutioner:
6. Polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-5.
7. Fremgangsmåde til fremstilling af en dej eller et spiseligt produkt fremstillet af dej, hvilken fremgangsmåde omfatter tilsætning af polypeptidet ifølge et hvilket som helst af kravene 1-5 til dejen.
DK10178829.7T 2003-05-09 2004-04-29 Lipolytiske enzymvarianter DK2270139T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200300709 2003-05-09
DKPA200300811 2003-05-30
US47964703P 2003-06-19 2003-06-19
EP04730201A EP1625216B1 (en) 2003-05-09 2004-04-29 Variant lipolytic enzymes

Publications (1)

Publication Number Publication Date
DK2270139T3 true DK2270139T3 (da) 2016-11-07

Family

ID=43500457

Family Applications (3)

Application Number Title Priority Date Filing Date
DK04730201.3T DK1625216T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter
DK10178829.7T DK2270139T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter
DK10178844.6T DK2270140T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK04730201.3T DK1625216T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK10178844.6T DK2270140T3 (da) 2003-05-09 2004-04-29 Lipolytiske enzymvarianter

Country Status (6)

Country Link
US (3) US7465570B2 (da)
EP (4) EP2270139B1 (da)
AU (2) AU2004236305B2 (da)
CA (1) CA2523400C (da)
DK (3) DK1625216T3 (da)
WO (1) WO2004099400A2 (da)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030051836A1 (en) * 2001-05-21 2003-03-20 Novozymes A/S Enzymatic hydrolysis of a polymer comprising vinyl acetate monomer
EP2270139B1 (en) * 2003-05-09 2016-07-27 Novozymes A/S Variant lipolytic enzymes
KR20090101930A (ko) 2006-12-21 2009-09-29 노보자임스 에이/에스 약학적 사용을 위한 리파아제 변형체
WO2010142695A1 (en) * 2009-06-10 2010-12-16 Novozymes A/S Phospholipases and methods of using same
NZ597118A (en) * 2009-06-25 2013-10-25 Dupont Nutrition Biosci Aps A method for increased expression of a variant lipolytic enzyme
EP2343310A1 (en) 2010-01-08 2011-07-13 Novozymes A/S Serine hydrolase formulation
CN102906251B (zh) 2010-04-26 2016-11-16 诺维信公司 酶颗粒剂
JP6002381B2 (ja) * 2010-12-07 2016-10-05 三菱化学フーズ株式会社 グリセロ糖脂質リパーゼを含有する小麦粉製品の製造方法
AR084876A1 (es) 2011-01-21 2013-07-10 Novozymes As Produccion de esteres alquilicos de acidos grasos
AR087146A1 (es) 2011-02-04 2014-02-26 Novozymes As Proceso para la preparacion de derivados de acidos carboxilicos
CN103459607A (zh) 2011-03-30 2013-12-18 诺维信公司 酯化反应法
DK2697356T3 (da) 2011-04-15 2020-09-07 Novozymes As Fremgangsmåde til fremstilling af ølurt
US10829721B2 (en) 2011-06-20 2020-11-10 Novozymes A/S Particulate composition
EP2847316A1 (en) 2012-05-11 2015-03-18 Novozymes A/S A brewing method
KR20150067336A (ko) 2012-10-12 2015-06-17 다니스코 유에스 인크. 지방분해 효소 변이체를 포함하는 조성물 및 방법
CN104718289A (zh) 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
DK2976423T3 (da) * 2013-03-21 2019-03-18 Novozymes As Polypeptider med phospholipase A-aktivitet og polynukleotider, som koder for dem
US20160160197A1 (en) * 2013-07-19 2016-06-09 Danisco Us Inc. Compositions and Methods Comprising a Lipolytic Enzyme Variant
US10155935B2 (en) 2014-03-12 2018-12-18 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP3131921B1 (en) * 2014-04-15 2020-06-10 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP3760713A3 (en) * 2014-05-27 2021-03-31 Novozymes A/S Lipase variants and polynucleotides encoding same
WO2016005315A1 (en) * 2014-07-07 2016-01-14 Novozymes A/S Dough with a lipolytic enzyme and/or xylanase and a monooygenase
US11124808B2 (en) * 2014-08-28 2021-09-21 Renescience A/S Solubilization of MSW with blend enzymes
EP3359679A1 (en) 2015-10-09 2018-08-15 Novozymes A/S Enzymatic or non-enzymatic biodiesel polishing process
DE102015224576A1 (de) * 2015-12-08 2017-06-08 Henkel Ag & Co. Kgaa Lipasen mit erhöhter Thermostabilität
EP3559222A1 (en) 2016-12-21 2019-10-30 DSM IP Assets B.V. Lipolytic enzyme variants
BR112019012559A2 (pt) 2016-12-21 2019-11-26 Dsm Ip Assets Bv variantes de enzima lipolítica
WO2018114938A1 (en) * 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) * 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CA3051770A1 (en) * 2017-02-20 2018-08-23 Novozymes A/S Lipolytic enzyme for use in baking
PE20211298A1 (es) * 2017-12-19 2021-07-20 Dupont Nutrition Biosci Aps Modificacion enzimatica mejorada de fosfolipidos en alimentos
CN108504643A (zh) * 2018-05-31 2018-09-07 华南理工大学 一种来源于米曲霉的偏甘油酯脂肪酶及其制备方法和晶体结构
EP3809852A1 (en) * 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
EP3937642A1 (en) 2019-03-13 2022-01-19 Novozymes A/S Production of par-baked products with improved freshness employing combination of gh8 xylanase and phospholipase
EP4156944A1 (en) 2020-05-29 2023-04-05 Novozymes A/S Pulse and/or legume protein-fortified doughs and baked goods comprising lipase
EP4334467A1 (en) 2021-05-04 2024-03-13 Novozymes A/S Enzymatic treatment of feedstock for hydrotreated vegetable oil (hvo) production
WO2023203080A1 (en) 2022-04-20 2023-10-26 Novozymes A/S Process for producing free fatty acids
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024046594A1 (en) 2022-09-01 2024-03-07 Novozymes A/S Baking with thermostable amg glucosidase variants (ec 3.2.1.3) and low or no added emulsifier
WO2024079301A1 (en) 2022-10-14 2024-04-18 Novozymes A/S Process for selective hydrolysis of diglycerides in an oil/fat with aid of candida antarctica lipase b

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6030488B2 (ja) 1982-11-10 1985-07-17 協和醗酵工業株式会社 生地の改良剤およびそれを含有してなる生地
JPS6078529A (ja) 1983-10-07 1985-05-04 協和醗酵工業株式会社 生地
JPS62111629A (ja) 1985-11-09 1987-05-22 キユーピー株式会社 ケ−キの製造方法
JPS63258528A (ja) 1987-04-15 1988-10-26 キユーピー株式会社 スポンジケ−キの製造方法
EP0426211B1 (en) 1989-09-29 1993-11-24 Unilever N.V. Food product containing dried lyso-phospholipoprotein
DE4017150A1 (de) 1990-05-28 1991-12-05 Henkel Kgaa Verfahren zur herstellung eines backaktiven pentosanase-praeparates
NL9001388A (nl) 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
NZ239083A (en) 1990-07-24 1993-08-26 Gist Brocades Nv Endo-xylanase-containing silage composition
ES2130173T3 (es) 1991-04-02 1999-07-01 Novo Nordisk As Xilanasa, secuencia correspondiente de adn recombinante, agente que contiene la xilanasa, y uso del agente.
WO1993013256A1 (en) 1991-12-20 1993-07-08 Novo Nordisk A/S Removal of hydrophobic esters from textiles
JP4307549B2 (ja) 1995-07-14 2009-08-05 ノボザイムス アクティーゼルスカブ 脂肪分解活性を有する修飾された酵素
WO1997004160A1 (en) 1995-07-19 1997-02-06 Novo Nordisk A/S Treatment of fabrics
CN1148442C (zh) 1996-12-09 2004-05-05 诺维信公司 利用来自具有磷脂酶a和/或b活性的丝状真菌的磷脂酶降低含有大量非水合磷的食用油中的含磷成分
ES2188143T5 (es) 1998-04-20 2008-11-01 Novozymes A/S Preparacion de masa y de productos horneados.
US6077336A (en) 1998-07-15 2000-06-20 Ulrich; Marcia A. Air filter system
EP1131416B1 (en) * 1998-11-27 2009-09-02 Novozymes A/S Lipolytic enzyme variants
EP2236611A1 (en) 2000-04-28 2010-10-06 Novozymes A/S Lipolytic enzyme variant
EP2281878A1 (en) 2000-06-26 2011-02-09 Novozymes A/S Lipolytic enzyme
ES2323947T3 (es) * 2001-01-10 2009-07-28 Novozymes A/S Variante de enzima lipolitica.
EP1468084A1 (en) * 2002-01-16 2004-10-20 Novozymes A/S Lipolytic enzyme variants and method for their production
EP2270139B1 (en) * 2003-05-09 2016-07-27 Novozymes A/S Variant lipolytic enzymes

Also Published As

Publication number Publication date
EP2270139A3 (en) 2011-11-23
AU2004236305A1 (en) 2004-11-18
DK2270140T3 (da) 2016-03-14
US7638317B2 (en) 2009-12-29
EP2270139B1 (en) 2016-07-27
AU2010246333A1 (en) 2010-12-09
US20080293120A1 (en) 2008-11-27
EP2270140A3 (en) 2011-11-30
EP2270140A2 (en) 2011-01-05
US20100047388A1 (en) 2010-02-25
AU2010241524A1 (en) 2010-12-09
EP2270140B1 (en) 2015-12-16
EP1625216A2 (en) 2006-02-15
CA2523400C (en) 2015-03-17
AU2010241524B2 (en) 2013-01-31
US20060228446A1 (en) 2006-10-12
DK1625216T3 (da) 2013-03-18
US20080311242A9 (en) 2008-12-18
WO2004099400A2 (en) 2004-11-18
EP2290057A3 (en) 2011-08-03
EP1625216B1 (en) 2012-11-21
WO2004099400A3 (en) 2004-12-16
EP2270139A2 (en) 2011-01-05
US7465570B2 (en) 2008-12-16
CA2523400A1 (en) 2004-11-18
EP2290057A2 (en) 2011-03-02
US8298800B2 (en) 2012-10-30
AU2004236305B2 (en) 2010-08-19

Similar Documents

Publication Publication Date Title
DK2270139T3 (da) Lipolytiske enzymvarianter
DK2295556T3 (da) Lipolytiske enzymvarianter og fremgangsmåde til fremstilling deraf.
US20120082756A1 (en) Preparation of Dough or Baked Products
MXPA02005452A (es) Metodo de mejorar la calidad de masa y pan.
WO2017142904A1 (en) Baking lipases
AU2017369532B2 (en) Method of baking
CA2766009A1 (en) Variant lipolytic enzymes with improved expression, functionality and/or activity
AU2008200576A1 (en) Lipolytic enzyme variants and method for their production
AU2001272359B2 (en) Method of preparing a dough or a baked product made from a dough, with addition of lipolytic enzymes
AU2001272359A1 (en) Method of preparing a dough or a baked product made from a dough, with addition of lipolytic enzymes