DK2258836T3 - Fremgangsmåder til forebyggelse, fjernelse, reduktion eller ødelæggelse af biofilm - Google Patents

Fremgangsmåder til forebyggelse, fjernelse, reduktion eller ødelæggelse af biofilm Download PDF

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DK2258836T3
DK2258836T3 DK10180231.2T DK10180231T DK2258836T3 DK 2258836 T3 DK2258836 T3 DK 2258836T3 DK 10180231 T DK10180231 T DK 10180231T DK 2258836 T3 DK2258836 T3 DK 2258836T3
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DK10180231.2T
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Randy Deinhammer
Carsten Andersen
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Novozymes North America Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B17/00—Methods preventing fouling
    • B08B17/02—Preventing deposition of fouling or of dust
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B7/00—Cleaning by methods not provided for in a single other subclass or a single group in this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
    • B08B9/027—Cleaning the internal surfaces; Removal of blockages
    • B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22002—Papain (3.4.22.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22004—Bromelain (3.4.22.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/2404—Serralysin (3.4.24.40)
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/20—Prevention of biofouling
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/04—Surfactants, used as part of a formulation or alone
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mechanical Engineering (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Claims (17)

1. Fremgangsmåde til forebyggelse, fjernelse, reduktion eller ødelæggelse af biofilm, som forefindes på en overflade, hvilken fremgangsmåde omfatter etablering af kontakt mellem overfladen og en alfa-amylase, som har sekvensen ifølge SEQ ID NO: 4, eller en alfa-amylase, som udviser mindst 90 % identitet med SEQ ID NO: 4.
2. Fremgangsmåde ifølge krav 1, hvor alfa-amylasen stammer fra en Bacillus-stamme.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor der etableres kontakt med den biofilmkontaminerede eller -modtagelige overflade i mellem 1 minut og 2 dage.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1-3, hvor der endvidere er et overfladeaktivt middel til stede.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor alfa-amylasen omfatter Asn-Gly-Thr-Met-Met-GIn-Tyr-Phe-Glu-Trp i sin N-terminale aminosyreregion.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1-5, hvor alfa-amylasen anvendes i en koncentration på 0,005-500 mg enzymprotein, fortrinsvis 0,01-100 mg enzymprotein pr. liter biofilmbekæmpelsesopløsning.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1-6, hvor alfa-amylasen har en procentdel (%) af hydrolyseret stivelse, som er højere end 15, fortrinsvis 25, især 35, efter 5 timer ved 40° C, 3 mg enzymprotein pr. g stivelse, pH 8,0.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1-7, hvor der er yderligere enzymer til stede, hvilke enzymer er valgt fra gruppen bestående af en aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextringlycosyltransferase, deoxyribonuklease, esterase, alfa-galactosidase, beta-galactosidase, glucoamylase, alfa-glucosidase, beta-glucosidase, haloperoxidase, invertase, laccase, lipase, mannosidase, oxidoreduktaser, pectinolytisk enzym, peptidoglutaminase, peroxidase, phytase, polyphenoloxidase, proteolytisk enzym, ribonuklease, transglutaminase og xylanase.
9. Fremgangsmåde ifølge krav 8, hvor det pectinolytiske enzym er valgt fra gruppen bestående af pectintranseliminase, polygalacturonase, pectinesterase.
10. Fremgangsmåde ifølge krav 9, hvor cellulasen er et flerkomponentcellulasepræparat eller en endoglucanase, fortrinsvis en Humicola-endoglucanase, især en Humicola insolens-endoglucanase, eller en endoglucanase, fortrinsvis en Γ/7/e/av/a-endoglucanase, fortrinsvis en Thielavia fe/resfr/'s-endoglucanase, eller en variant deraf.
11. Fremgangsmåde ifølge krav 10, hvor det proteolytiske enzym er en protease, fortrinsvis en serinprotease, især en, der stammer fra en Sac/V/i/s-stamme, såsom Bacillus lentus eller Bacillus clausii, eller en variant deraf.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 1-11, hvor der endvidere er et eller flere midler valgt fra gruppen bestående af dispergeringsmidler, overfladeaktive midler, antimikrobielle midler og biocider til stede.
13. Fremgangsmåde ifølge et hvilket som helst af kravene 1-12, hvor overfladen er en hård, blød eller porøs overflade.
14. Fremgangsmåde ifølge krav 13, hvor overfladen er en membran.
15. Fremgangsmåde ifølge et hvilket som helst af kravene 1-14, hvor biofilmfjernelsen udføres ved en temperatur på 10-70° C, fortrinsvis 40-60° C.
16. Anvendelse af en alfa-amylase ifølge et hvilket som helst af kravene 1-15 til forebyggelse eller fjernelse af biofilm fra overflader.
17. Anvendelse ifølge krav 16, hvor der anvendes yderligere enzymer og/eller midler ifølge krav 1-15.
DK10180231.2T 2004-09-10 2005-09-07 Fremgangsmåder til forebyggelse, fjernelse, reduktion eller ødelæggelse af biofilm DK2258836T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60853504P 2004-09-10 2004-09-10
EP05795624.5A EP1791966B1 (en) 2004-09-10 2005-09-07 Methods for preventing, removing, reducing, or disrupting biofilm

Publications (1)

Publication Number Publication Date
DK2258836T3 true DK2258836T3 (da) 2016-07-25

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US (4) US20080248558A1 (da)
EP (3) EP1791966B1 (da)
JP (1) JP5345781B2 (da)
CN (4) CN103181400B (da)
DK (1) DK2258836T3 (da)
WO (1) WO2006031554A2 (da)

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