DK2251421T3 - Stabiliseret transglutaminase og fremgangsmåde til fremstilling deraf - Google Patents

Stabiliseret transglutaminase og fremgangsmåde til fremstilling deraf Download PDF

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Publication number
DK2251421T3
DK2251421T3 DK09711348.4T DK09711348T DK2251421T3 DK 2251421 T3 DK2251421 T3 DK 2251421T3 DK 09711348 T DK09711348 T DK 09711348T DK 2251421 T3 DK2251421 T3 DK 2251421T3
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DK
Denmark
Prior art keywords
transglutaminase
tgase
stabilized
pro
microorganism
Prior art date
Application number
DK09711348.4T
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English (en)
Inventor
Masamichi Okada
Yoshiaki Kurono
Hitoshi Amano
Shotaro Yamaguchi
Original Assignee
Amano Enzyme Inc
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Publication date
Application filed by Amano Enzyme Inc filed Critical Amano Enzyme Inc
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Publication of DK2251421T3 publication Critical patent/DK2251421T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

Claims (13)

1. Stabiliseret transglutaminase omfattende et transglutaminaseprosekvenspeptid og en moden transglutaminase, hvor transglutaminaseprosekvenspeptidet er bundet til den modne transglutaminase, og den stabiliserede transglutaminase har højere stabilitet end den modne transglutaminase, hvortil prosekvenspeptidet ikke er bundet, og stabiliteten er mindst én stabilitetsform udvalgt fra gruppen bestående af pH-stabilitet, temperaturstabilitet, stabilitet mod oxidation og lagerstabilitet.
2. Stabiliseret transglutaminase ifølge krav 2, hvor den modne transglutaminase er afledt af en mikroorganisme.
3. Stabiliseret transglutaminase ifølge krav 2, hvor mikroorganismen er en mikroorganisme af slægten Streptomyces.
4. Stabiliseret transglutaminase ifølge krav 2, hvor mikroorganismen er Streptomyces mobaraensis.
5. Stabiliseret transglutaminase ifølge krav 1, hvor den modne transglutaminase indbefatter en aminosyresekvens gengivet i SEQ ID NO:6.
6. Stabiliseret transglutaminase ifølge krav 1, hvor prosekvenspeptidet er afledt af en mikroorganisme.
7. Stabiliseret transglutaminase ifølge krav 6, hvor mikroorganismen er en mikroorganisme af slægten Streptomyces.
8. Stabiliseret transglutaminase ifølge krav 6, hvor mikroorganismen er Streptomyces mobaraensis.
9. Stabiliseret transglutaminase ifølge krav 1, hvor prosekvenspeptidet indbefatter en hvilken som helst af følgende sekvenser (1) til (3): (1) DNGAGEETKSYAETYRLTADDVANINALNESAPAA (SEQ ID NO: 1); (2) DNGAGEETKSYAETYRLTADDVANINALNESAPAAS (SEQ ID NO: 2); og (3) DNGAGEETKSYAETYRLTADDVANINALNESAPAASS (SEQ ID NO: 3).
10. Fremgangsmåde til fremstilling af en stabiliseret transglutaminase, hvilken fremgangsmåde omfatter følgende trin: at dyrke en mikroorganisme, der er i stand til at danne transglutaminase under betingelser, hvor transglutaminase kan dannes; og at separere og indvinde en moden transglutaminase, hvortil prosekvenspeptid er bundet, fra en kulturopløsning.
11. Fremgangsmåde til fremstilling af en stabiliseret transglutaminase ifølge krav 10, hvor mikroorganismen er en mikroorganisme af slægten Streptomyces.
12. Fremgangsmåde til fremstilling af stabiliseret transglutaminase ifølge krav 10, hvor mikroorganismen er Streptomyces mobaraensis.
13. Anvendelse af den stabiliserede transglutaminase ifølge et hvilket som helst af kravene 1 til 9 til binding af kød, fremstilling af pølser, tofu, brød eller nudler.
DK09711348.4T 2008-02-13 2009-01-22 Stabiliseret transglutaminase og fremgangsmåde til fremstilling deraf DK2251421T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008032252 2008-02-13
PCT/JP2009/000221 WO2009101762A1 (ja) 2008-02-13 2009-01-22 安定型トランスグルタミナーゼ及びその製造法

Publications (1)

Publication Number Publication Date
DK2251421T3 true DK2251421T3 (da) 2017-02-06

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DK09711348.4T DK2251421T3 (da) 2008-02-13 2009-01-22 Stabiliseret transglutaminase og fremgangsmåde til fremstilling deraf

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US (3) US20100297726A1 (da)
EP (1) EP2251421B1 (da)
JP (1) JP5580056B2 (da)
CN (1) CN101945995B (da)
DK (1) DK2251421T3 (da)
ES (1) ES2616020T3 (da)
WO (1) WO2009101762A1 (da)

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CN103748207A (zh) 2011-08-25 2014-04-23 天野酶株式会社 提高泡沫稳定性的组合物及其用途
FI126442B (en) * 2011-11-01 2016-12-15 Valio Oy Liquid enzyme preparation and process for its preparation
CN102533689A (zh) * 2011-12-30 2012-07-04 华东师范大学 一种谷氨酰胺转胺酶真空干燥的制备方法
EP3355934A1 (en) * 2015-10-02 2018-08-08 Bristol-Myers Squibb Company Transglutaminase variants for conjugating antibodies
CN107326019B (zh) * 2016-02-16 2018-05-01 上海青瑞食品科技有限公司 一种液体酶制剂及制备方法
CN108018279B (zh) * 2018-01-24 2020-09-04 江南大学 一种谷氨酰胺转胺酶复配剂及其应用
CN111593011B (zh) * 2020-07-07 2021-12-24 上海东之汇生物科技有限公司 一种转谷氨酰胺酶生产菌
CN114686410B (zh) * 2020-12-25 2023-11-21 泰兴市东圣生物科技有限公司 一种增强1-磷酸果糖激酶基因转录水平的谷氨酰胺转氨酶高产菌株及其制备与发酵方法
CN117940561A (zh) 2021-10-04 2024-04-26 天野酶制品株式会社 液态转谷氨酰胺酶酶制剂
WO2023225666A1 (en) * 2022-05-20 2023-11-23 Curie Co. Inc. Pro-peptide variants for modulating activity of transglutaminases

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JPH0665280B2 (ja) 1987-03-04 1994-08-24 味の素株式会社 タンパクゲル化剤及びそれを用いるタンパクのゲル化方法
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Also Published As

Publication number Publication date
ES2616020T3 (es) 2017-06-09
US9487765B2 (en) 2016-11-08
CN101945995B (zh) 2013-06-12
JPWO2009101762A1 (ja) 2011-06-09
EP2251421B1 (en) 2016-11-16
WO2009101762A1 (ja) 2009-08-20
CN101945995A (zh) 2011-01-12
JP5580056B2 (ja) 2014-08-27
US20160040141A1 (en) 2016-02-11
EP2251421A1 (en) 2010-11-17
US20100297726A1 (en) 2010-11-25
US20150176000A1 (en) 2015-06-25
US9217141B2 (en) 2015-12-22
EP2251421A4 (en) 2011-08-31

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