DK2173861T4 - Fremgangsmåder til behandling af et cellekulturmedium til anvendelse i en bioreaktor - Google Patents

Fremgangsmåder til behandling af et cellekulturmedium til anvendelse i en bioreaktor Download PDF

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Publication number
DK2173861T4
DK2173861T4 DK08770870.7T DK08770870T DK2173861T4 DK 2173861 T4 DK2173861 T4 DK 2173861T4 DK 08770870 T DK08770870 T DK 08770870T DK 2173861 T4 DK2173861 T4 DK 2173861T4
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Denmark
Prior art keywords
cell culture
culture medium
culture media
bioreactor
methods
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DK08770870.7T
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English (en)
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DK2173861T3 (da
Inventor
Joe Zhou
Felix M Solamo
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Amgen Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A61L2/0017—Filtration

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (11)

  1. PAT E NT K RAV
    1. Fremgangsmåde til behandling af et cellekulturmedium til anvendelse i en bioreaktor, omfattende: (a) at udsætte cellekulturmediet for ultraviolet C (UVC) lys; (a2) at lede cellekulturmediet gennem et dybdefilter; (b) at lede cellekulturmediet gennem et sterilt filter; og (c) at indføre cellekulturmediet i en bioreaktor.
  2. 2. Fremgangsmåde ifølge krav 1, hvor UVC lyset har en bølgelængde på 254 nm.
  3. 3. Fremgangsmåde ifølge krav 1, hvor cellekulturmediet udsættes for UVC lys ved en strømningshastighed på 1 - 12 liter pr. time, fortrinsvis 6 liter pr. time.
  4. 4. Fremgangsmåde ifølge krav 1, hvor logreduktionsværdien er større end eller lig med 4,85, idet logreduktionsværdien fortrinsvis er 6 - 7.
  5. 5. Fremgangsmåde ifølge krav 1, hvor cellekulturmediet udsættes for UVC lys ved en energidensitet på 120 - 320 J/m2, fortrinsvis 238 J/m2.
  6. 6. Fremgangsmåde ifølge krav 1, hvor det sterile filter har porer med en maksimumsstørrelse på 200 nm.
  7. 7. Fremgangsmåde ifølge krav 1, hvor trinet med at udsætte cellekulturmediet for UVC lys er tilstrækkeligt til at beskadige nucleinsyrerne af eventuelt ikke-indhyllede virusser i cellekulturmediet.
  8. 8. Fremgangsmåde ifølge krav 1, hvor det behandlede cellekulturmedium anvendes til at støtte væksten af pattedyrceller eller insektceller.
  9. 9. Fremgangsmåde ifølge krav 8, hvor pattedyrcellerne kan producere antistoffer.
  10. 10. Fremgangsmåde ifølge krav 1, hvor cellekulturmediet ledes gennem dybdefiltret ved en sur pH, fortrinsvis pH 5,0.
  11. 11. Fremgangsmåde ifølge krav 1, hvor trinet med at lede cellekulturmediet gennem et dybdefilter er tilstrækkeligt til at fjerne eventuelt indhyllede virusser fra cellekulturmediet.
DK08770870.7T 2007-06-15 2008-06-12 Fremgangsmåder til behandling af et cellekulturmedium til anvendelse i en bioreaktor DK2173861T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94446807P 2007-06-15 2007-06-15
PCT/US2008/066745 WO2008157247A1 (en) 2007-06-15 2008-06-12 Methods of treating cell culture media for use in a bioreactor

Publications (2)

Publication Number Publication Date
DK2173861T3 DK2173861T3 (da) 2011-07-25
DK2173861T4 true DK2173861T4 (da) 2017-05-15

Family

ID=39722676

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08770870.7T DK2173861T4 (da) 2007-06-15 2008-06-12 Fremgangsmåder til behandling af et cellekulturmedium til anvendelse i en bioreaktor

Country Status (21)

Country Link
US (1) US9320816B2 (da)
EP (1) EP2173861B2 (da)
JP (1) JP5579599B2 (da)
KR (1) KR101307697B1 (da)
CN (1) CN101821381B (da)
AT (1) ATE506432T2 (da)
AU (1) AU2008266131B2 (da)
BR (1) BRPI0813368A2 (da)
CA (1) CA2690673C (da)
CY (1) CY1111875T1 (da)
DE (1) DE602008006410D1 (da)
DK (1) DK2173861T4 (da)
EA (1) EA017322B1 (da)
ES (1) ES2362990T5 (da)
HK (1) HK1143184A1 (da)
IL (1) IL202542A0 (da)
MX (1) MX2009013762A (da)
PL (1) PL2173861T5 (da)
PT (1) PT2173861E (da)
WO (1) WO2008157247A1 (da)
ZA (1) ZA201000144B (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2173861B2 (en) 2007-06-15 2017-02-22 Amgen Inc. Methods of treating cell culture media for use in a bioreactor
US20130039943A1 (en) * 2010-05-03 2013-02-14 Bruno Rene Andre Novel method
WO2012115874A1 (en) * 2011-02-23 2012-08-30 Amgen Inc. Cell culture media for uvc exposure and methods related thereto
BR112014010027A2 (pt) * 2011-10-26 2017-04-25 Amgen Inc métodos de redução ou eliminação de modificação e degradação de proteínas decorrentes de exposição à luz uv
ITNA20120046A1 (it) * 2012-08-02 2014-02-03 No Self S R L Uso di acidi nucleici di sistemi biologici parassiti, patogeni e infestanti per l'inibizione e/o controllo degli stessi sistemi
JP6695814B2 (ja) 2014-06-04 2020-05-20 アムジエン・インコーポレーテツド 哺乳類細胞培養物を回収するための方法
EA036178B1 (ru) 2014-12-01 2020-10-09 Эмджен Инк. Процесс контроля уровня содержания гликанов в составе гликопротеинов
CN107496950B (zh) * 2017-08-18 2021-02-02 广州市众为生物技术有限公司 一种紫外线照射灭活蛋白溶液中病毒的方法
US11771814B2 (en) 2018-07-27 2023-10-03 Terumo Bct Biotechnologies, Llc Flow cell
AU2021260878A1 (en) 2020-04-20 2022-11-17 Vestaron Corporation Proteolytically stable U1-agatoxin-Ta1b variant polypeptides for pest control
TW202205956A (zh) 2020-05-01 2022-02-16 美商韋斯塔隆公司 殺蟲組合
US20230287335A1 (en) 2020-07-30 2023-09-14 Amgen Inc. Cell culture media and methods of making and using the same
IL301655A (en) 2020-09-28 2023-05-01 Vestaron Corp MU-Digotoxin-DC1A variant polypeptides for pest control
CA3215602A1 (en) 2021-04-01 2022-10-06 Vestaron Corporation Av3 mutant polypeptides for pest control
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
WO2023192924A1 (en) 2022-03-30 2023-10-05 Vestaron Corporation Combinations of av3 mutant polypeptides and bt toxins for pest control
TW202413392A (zh) 2022-05-18 2024-04-01 美商韋斯塔隆公司 殺蟲ａｃｔｘ肽變異體
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9500724D0 (sv) 1994-06-23 1995-02-24 Pharmacia Ab Filtrering
US6090588A (en) * 1997-05-02 2000-07-18 Board Of Regents Of University Of Nebraska Isolated melanin-like substance and method for producing the same
US6995006B2 (en) * 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6214221B1 (en) * 1999-02-22 2001-04-10 Henry B. Kopf Method and apparatus for purification of biological substances
US20030127603A1 (en) * 2001-05-15 2003-07-10 Bernard Horowitz Apparatus for the inactivation of pathogens in protein-containing fluids and uses thereof
CN1259139C (zh) * 2001-10-10 2006-06-14 诺利塔克股份有限公司 在选择地使生物学有害物质灭活的光催化材料及其利用
US20040214314A1 (en) * 2001-11-02 2004-10-28 Friedrich Srienc High throughput bioreactor
WO2003094978A1 (en) * 2002-05-08 2003-11-20 Millipore Corporation Light decontamination of fermentation media
US20050196854A1 (en) * 2002-05-14 2005-09-08 Konz John O.Jr. Methods of adenovirus purification
WO2004075931A2 (en) * 2003-02-27 2004-09-10 Baxter International Inc. Method for the validatable inactivation of pathogens in a biological fluid by irradiation
DE10312765A1 (de) 2003-03-21 2004-09-30 Bayer Technology Services Gmbh Vorrichtung und Verfahren zur Sterilisation flüssiger Medien mittels UV-Bestrahlung und Kurzzeiterhitzung
EP1624950B1 (en) * 2003-05-19 2007-07-11 Millipore Corporation Process for prefiltration of a protein solution
US20090130704A1 (en) * 2003-11-13 2009-05-21 Gyure Dale C Novel bioreactor
US8703467B2 (en) * 2004-05-27 2014-04-22 Baxter International Inc. Inactivation of a pathogen in a sample by a treatment with formalin and UV light
KR101483337B1 (ko) 2004-05-27 2015-01-15 백스터 인터내셔널 인코포레이티드 포르말린 및 ｕｖ광으로의 처리에 의한 샘플 중 병원균의 불활성화
US7476885B2 (en) * 2006-02-22 2009-01-13 Oreck Corporation Disinfecting device utilizing ultraviolet radiation
DK2007883T3 (da) * 2006-04-20 2012-02-06 Wyeth Llc Rensningsfremgangsmåder til isolering af renset vesikulær stomatitis-virus fra cellekultur
EP2173861B2 (en) 2007-06-15 2017-02-22 Amgen Inc. Methods of treating cell culture media for use in a bioreactor

Also Published As

Publication number Publication date
HK1143184A1 (en) 2010-12-24
AU2008266131A1 (en) 2008-12-24
US20100203610A1 (en) 2010-08-12
ZA201000144B (en) 2010-09-29
CN101821381B (zh) 2012-09-05
ES2362990T3 (es) 2011-07-18
BRPI0813368A2 (pt) 2015-01-06
EP2173861B1 (en) 2011-04-20
WO2008157247A1 (en) 2008-12-24
IL202542A0 (en) 2011-08-01
KR101307697B1 (ko) 2013-09-11
KR20100036319A (ko) 2010-04-07
EA017322B1 (ru) 2012-11-30
JP2010529856A (ja) 2010-09-02
US9320816B2 (en) 2016-04-26
ES2362990T5 (es) 2017-07-20
DK2173861T3 (da) 2011-07-25
CA2690673C (en) 2013-04-23
PL2173861T5 (pl) 2017-10-31
CA2690673A1 (en) 2008-12-24
EA201000020A1 (ru) 2010-06-30
DE602008006410D1 (de) 2011-06-01
ATE506432T2 (de) 2011-05-15
PL2173861T3 (pl) 2011-09-30
PT2173861E (pt) 2011-05-13
MX2009013762A (es) 2010-03-01
JP5579599B2 (ja) 2014-08-27
CY1111875T1 (el) 2016-02-10
EP2173861B2 (en) 2017-02-22
CN101821381A (zh) 2010-09-01
AU2008266131B2 (en) 2011-10-20
EP2173861A1 (en) 2010-04-14

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