DK1781772T3 - Metabolisk modificering af xylose-fermenterende eukaryote celler - Google Patents

Metabolisk modificering af xylose-fermenterende eukaryote celler Download PDF

Info

Publication number
DK1781772T3
DK1781772T3 DK05763114.5T DK05763114T DK1781772T3 DK 1781772 T3 DK1781772 T3 DK 1781772T3 DK 05763114 T DK05763114 T DK 05763114T DK 1781772 T3 DK1781772 T3 DK 1781772T3
Authority
DK
Denmark
Prior art keywords
host cell
ala
leu
lys
gly
Prior art date
Application number
DK05763114.5T
Other languages
English (en)
Inventor
Aaron Adriaan Winkler
Sipko Maarten Kuyper
Laat Wilhelmus Theodorus Antonius Maria De
Dijken Johannes Pieter Van
Jacobus Thomas Pronk
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of DK1781772T3 publication Critical patent/DK1781772T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (21)

1. Gær eller trådformet svampeværtscelle, der er transformeret med en nukleinsyrekonstruktion, der omfatter en nukleotidsekvens, der koder for en xyloseisomerase, hvilken xyloseisomerase omfatter en aminosyresekvens, der har mindst 95 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 2, hvor nukleinsyrekonstruktionen ved transformation af værtscellen bibringer værtscellen evnen til direkte isomerisering af xylose til xylulose, hvor værtscellen omfatter en genmodifikation, der øger strømningen via pentosephosphat-reaktionsvejen, og hvor genmodifikationen omfatter overekspression af mindst ét gen i den ikke-oxidative del af pentosephosphat-reaktionsvejen sammenlignet med en værtscelle, der er genetisk identisk bortset fra genmodifikationen.
2. Værtscelle ifølge krav 1, hvor nukleotidsekvensen koder for en xyloseisomerase, der kan opnås fra en bakterie fra klassen Bacteroides.
3. Værtscelle ifølge krav 1 eller 2, hvor genet i den ikke-oxidative del af pentosephosphat-reaktionsvejen er udvalgt fra gruppen, der består af generne, der koder for ribulose-5-phosphatisomerase, ribulose-5-phosphatepimerase, transketolase og transaldolase.
4. Værtscelle ifølge krav 3, hvor genmodifikationen omfatter overekspression af mindst generne, der koder for en transketolase og en transaldolase.
5. Værtscelle ifølge et hvilket som helst af kravene 1-4, hvor værtscellen yderligere omfatter en genmodifikation, der øger den specifikke xylulosekinaseaktivitet.
6. Værtscelle ifølge krav 5, hvor genmodifikationen omfatter overekspression af et gen, der koder for en xylulosekinase.
7. Værtscelle ifølge et hvilket som helst af kravene 1-6, hvor genet, der overudtrykkes, er endogent for værtscellen.
8. Værtscelle ifølge et hvilket som helst af kravene 1-7, hvor værtscellen omfatter en genmodifikation, der reducerer uspecifik aldosereductaseaktivitet i værtscellen.
9. Værtscelle ifølge krav 8, hvor genmodifikationen reducerer ekspressionen af eller inaktiverer et gen, der koder for en uspecifik aldosereductase.
10. Værtscelle ifølge krav 9, hvor genet er inaktiveret ved deletion af mindst en del af genet eller ved destruktion af genet.
11. Værtscelle ifølge krav 8 eller 9, hvor ekspressionen af hvert gen i værtscellen, der koder for en uspecifik aldosereductase, er reduceret eller inaktiveret.
12. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen er en gær, fortrinsvis en gær, der tilhører en af slægterne: Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces og Yarrowia.
13. Værtscelle ifølge krav 12, hvor gæren tilhører en af arterne: S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, K. lactis, K. marxianus og K. fragilis.
14. Værtscelle ifølge et hvilket som helst af kravene 1-11, hvor værtscellen er en trådformet svamp, fortrinsvis en trådformet svamp, der tilhører en af slægterne: Aspergillus, Trichoderma, Humicola, Acremonium, Fusarium og Penicillium.
15. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen udtrykker et eller flere enzymer, der bibringer værtscellen evnen til at danne mindst ét fermenteringsprodukt udvalgt fra gruppen, der består af ethanol, mælkesyre, 3-hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner.
16. Fremgangsmåde til fremstillng af ethanol, hvilken fremgangsmåde omfatter trinene: (a) fermentering af et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge et hvilket som helst af kravene 1-14, hvor værtscellen fermenterer xylose til ethanol, og eventuelt (b) indvinding af ethanol.
17. Fremgangsmåde ifølge krav 16, hvor mediet også indeholder en kilde af glucose.
18. Fremgangsmåde ifølge krav 16 eller 17, hvor den volumetriske produktivitet af ethanol er mindst 0,5 g ethanol pr. liter pr. time.
19. Fremgangsmåde ifølge et hvilket som helst af kravene 16-18, hvor ethanoludbyttet er mindst 50 %.
20. Fremgangsmåde til fremstilling af et fermenteringsprodukt udvalgt fra gruppen, der består af mælkesyre, 3- hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner, hvilken fremgangsmåde omfatter trinene: (a) fermentering at et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge krav 15, hvor værtscellen fermenterer xylose til fermenteringsproduktet, og eventuelt (b) indvinding af fermenteringproduktet.
21. Fremgangsmåde ifølge krav 20, hvor mediet også indeholder en kilde af glucose.
DK05763114.5T 2004-07-16 2005-07-15 Metabolisk modificering af xylose-fermenterende eukaryote celler DK1781772T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58838104P 2004-07-16 2004-07-16
EP04077073 2004-07-16
US58983304P 2004-07-22 2004-07-22
PCT/NL2005/000516 WO2006009434A1 (en) 2004-07-16 2005-07-15 Metabolic engineering of xylose fermenting eukaryotic cells

Publications (1)

Publication Number Publication Date
DK1781772T3 true DK1781772T3 (da) 2017-01-09

Family

ID=34928378

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05763114.5T DK1781772T3 (da) 2004-07-16 2005-07-15 Metabolisk modificering af xylose-fermenterende eukaryote celler

Country Status (10)

Country Link
US (3) US8034591B2 (da)
EP (1) EP1781772B1 (da)
JP (2) JP5796925B2 (da)
CN (2) CN101914462B (da)
BR (1) BRPI0513437A (da)
CA (2) CA2573483C (da)
DK (1) DK1781772T3 (da)
ES (1) ES2607891T3 (da)
PL (1) PL1781772T3 (da)
WO (1) WO2006009434A1 (da)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0306740B1 (pt) 2002-01-23 2019-08-27 C5 Yeast Company B V célula hospedeira transformada com um construto de ácido nucléico e processo para a produção de etanol
BRPI0513437A (pt) * 2004-07-16 2008-05-06 Univ Delft Tech célula hospedeira eucariótica com a capacidade para isomerizar diretamente xilose em xilulose, e, processos para produzir etanol e um produto de fermentação
EP1873246A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a transaldolase
EP1873248A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a glucose-6-phosphate isomerase
CA2664646C (en) * 2006-10-02 2016-09-20 Dsm Ip Assets B.V. Metabolic engineering of arabinose- fermenting yeast cells
CN103289908B (zh) * 2006-10-02 2018-05-11 帝斯曼知识产权资产管理有限公司 发酵阿拉伯糖的真核细胞的代谢工程改造
WO2008144012A2 (en) * 2007-05-14 2008-11-27 The Regents Of The University Of California Xylose- and arabinose- metabolizing enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same
US20100304454A1 (en) * 2007-07-19 2010-12-02 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
EP2062967A1 (en) 2007-11-21 2009-05-27 Technical University of Denmark Genetically engineered aspergillus
US20110189728A1 (en) * 2008-03-07 2011-08-04 Paul Klaassen Pentose sugar fermenting cell
WO2009109630A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109631A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
US8399215B2 (en) * 2008-03-07 2013-03-19 Dsm Ip Assets B.V. Pentose sugar fermenting cell
GB0812318D0 (en) 2008-07-04 2008-08-13 Terranol As Microorganism
WO2010014817A2 (en) * 2008-07-30 2010-02-04 Novozymes A/S Producing fermentation products
CA3092340A1 (en) * 2008-11-21 2010-05-27 Universiteit Stellenbosch Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
US9334488B2 (en) * 2008-12-24 2016-05-10 Dsm Ip Assets B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
KR20110124781A (ko) * 2009-02-23 2011-11-17 기린 홀딩스 가부시키가이샤 자일로오스를 탄소원으로 사용할 수 있는, 캔디다 유틸리스에 의한 물질의 제조법
CA2753538A1 (en) 2009-02-25 2010-09-02 Indiana University Research & Technology Corporation Materials and methods for identifying and using yeast strains that metabolize pentose sugars in the presence of d-glucose
US8679782B2 (en) 2009-06-15 2014-03-25 Massachusetts Institute Of Technology Production of triacylglycerides, fatty acids, and their derivatives
US20120184020A1 (en) * 2009-07-09 2012-07-19 Verdezyne, Inc. Engineered microorganisms with enhanced fermentation activity
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
WO2011065539A1 (ja) * 2009-11-30 2011-06-03 国立大学法人神戸大学 バイオマスからのエタノールの生産方法
CA2785276C (en) * 2009-12-22 2019-04-23 Kabushiki Kaisha Toyota Chuo Kenkyusho Xylose isomerase and use thereof
PL2561064T3 (pl) 2010-04-21 2018-03-30 Dsm Ip Assets B.V. Komórka odpowiednia do fermentacji kompozycji mieszanych cukrów
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
WO2011153516A2 (en) 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
US8679822B2 (en) * 2010-06-29 2014-03-25 E I Du Pont De Nemours And Company Xylose utilization in recombinant zymomonas
WO2012019175A2 (en) 2010-08-06 2012-02-09 Mascoma Corporation Production of malonyl-coa drived products via anaerobic pathways
CN102399804A (zh) * 2010-09-15 2012-04-04 中国农业科学院作物科学研究所 木糖异构酶基因的功能及其应用
JP5961886B2 (ja) 2010-10-13 2016-08-03 ディーエスエム アイピー アセッツ ビー．ブイ． ペントースおよびグルコース発酵酵母細胞
ES2532508T3 (es) 2010-11-18 2015-03-27 Dsm Ip Assets B.V. Cepas de levaduras manipuladas para producir etanol a partir de glicerol
JP2012120491A (ja) * 2010-12-09 2012-06-28 Toyota Motor Corp キシロースを含む培地における発酵培養方法
WO2012088467A2 (en) 2010-12-22 2012-06-28 Mascoma Corporation Genetically modified clostridium thermocellum engineered to ferment xylose
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
WO2012138942A1 (en) 2011-04-05 2012-10-11 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
AR086471A1 (es) 2011-04-22 2013-12-18 Dsm Ip Assets Bv Celula de levadura capaz de convertir azucares que incluyen arabinosa y xilosa
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
EP2785849B1 (en) 2011-11-30 2017-09-27 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
DK2806754T3 (da) 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpen-fremstilling
ES2698500T3 (es) * 2012-03-06 2019-02-05 Lygos Inc Células huésped recombinantes para la producción de malonato
JP6249391B2 (ja) * 2012-06-15 2017-12-20 国立研究開発法人農業・食品産業技術総合研究機構 キシロースを高温で発酵する方法
IN2015DN01041A (da) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
CN104797708A (zh) 2012-08-29 2015-07-22 拉勒曼德匈牙利流动性管理有限责任公司 酵母中用于木质纤维素衍生的寡聚物cbp的酶的表达
US10604773B2 (en) 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
ES2717682T3 (es) * 2012-10-16 2019-06-24 Dsm Ip Assets Bv Células con conversión mejorada de pentosas
CN104769099A (zh) 2012-11-07 2015-07-08 帝斯曼知识产权资产管理有限公司 pH控制的酵母繁殖
US20140256011A1 (en) 2012-11-09 2014-09-11 Mascoma Corporation Method for Acetate Consumption During Ethanolic Fermentaion of Cellulosic Feedstocks
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014160402A1 (en) 2013-03-14 2014-10-02 Mascoma Corporation Co-conversion of carbohydrates to fermentation products in a single fermentation step
DE102013205986A1 (de) 2013-04-04 2014-10-23 Wacker Chemie Ag Mikroorganismenstamm und Verfahren zur fermentativen Herstellung von C4-Verbindungen aus C5-Zuckern
DK3004366T3 (da) 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
EP3885443A1 (en) 2013-07-15 2021-09-29 DSM IP Assets B.V. Diterpene production
US20160185813A1 (en) 2013-07-31 2016-06-30 Dsm Ip Assets B.V. Recovery of steviol glycosides
MX2016001881A (es) 2013-08-15 2016-08-03 Lallemand Hungary Liquidity Man Llc Metodos para la mejora de rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol.
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
EP3770267A1 (en) * 2013-11-04 2021-01-27 The Board of Trustees of the Leland Stanford Junior University Benzylisoquinoline alkaloid (bia) precursor producing microbes, and methods of making and using the same
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
BR102014014407A2 (pt) * 2014-06-12 2016-04-19 Biocelere Agroindustrial Ltda cassete de expressão para transformar célula eucariótica, micro-organismo geneticamente modificado com eficiente consumo de xilose, processo para produção de biocombustíveis e bioquímicos e biocombustível e/ou bioquímico assim produzido
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
BR102014027233A2 (pt) * 2014-10-30 2016-05-24 Biocelere Agroindustrial Ltda cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos
MY182182A (en) 2015-01-28 2021-01-18 Dsm Ip Assets Bv Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA2973302C (en) 2015-01-28 2022-10-25 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN115851470A (zh) 2015-03-16 2023-03-28 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
EP3274448B1 (en) 2015-03-23 2021-08-11 DSM IP Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
CN107666834B (zh) 2015-04-03 2021-08-24 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA2980649A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthase
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN107820394A (zh) 2015-07-10 2018-03-20 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷组合物
JP6977231B2 (ja) 2015-07-13 2021-12-08 マラ リニューアブルズ コーポレーション Ｃ５有機炭素の微生物による代謝の増強
EP3332004A1 (en) 2015-08-05 2018-06-13 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
CN107922913B (zh) 2015-08-13 2022-04-05 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷转运
CN106554924B (zh) * 2015-09-24 2019-12-06 中粮营养健康研究院有限公司 生产乙醇的重组酿酒酵母菌株、其构建方法以及利用该菌株生产乙醇的方法
EP4043558A3 (en) 2015-10-05 2022-11-16 DSM IP Assets B.V. Kaurenoic acid hydroxylases
US10570421B2 (en) * 2015-11-06 2020-02-25 Lallemand Hungary Liquidity Management Llc Limiting yeast-produced trehalose in fermentation
CN105838744A (zh) * 2016-04-26 2016-08-10 上海交通大学 制备2-表-5-表有效醇酮的方法
BR112018074561A2 (pt) 2016-06-09 2019-03-12 Dsm Ip Assets Bv trem de semente para produção de enzima em grande escala
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018029274A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
CA3032656A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
WO2018078014A1 (en) 2016-10-27 2018-05-03 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
CA3040380A1 (en) 2016-11-04 2018-05-11 Inbicon A/S Method for preparing fermentable sugars from lignocellulosic biomass
WO2018096017A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme composition
EP3545100A1 (en) 2016-11-24 2019-10-02 DSM IP Assets B.V. Enzyme composition
WO2018104238A1 (en) 2016-12-08 2018-06-14 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2018115251A1 (en) 2016-12-21 2018-06-28 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
WO2018115513A1 (en) * 2016-12-22 2018-06-28 Lallemand Hungary Liquidity Management Llc Sulfite tolerance in recombinant yeast host cells
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN110892068B (zh) 2017-06-27 2024-03-08 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
CA3073387A1 (en) * 2017-08-29 2019-03-07 Danisco Us Inc Modified yeast comprising glucose-specific, atp-mediated transporters
CN111225982A (zh) 2017-10-09 2020-06-02 帝斯曼知识产权资产管理有限公司 用于木质纤维素材料的酶促水解和糖发酵的方法
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
BR112020007246A2 (pt) 2017-10-30 2020-10-13 Dsm Ip Assets B.V. processo para hidrólise enzimática de material lignocelulósico e fermentação de açúcares
US11193145B2 (en) 2018-03-28 2021-12-07 Dsm Ip Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
CN112424218A (zh) 2018-04-30 2021-02-26 帝斯曼知识产权资产管理有限公司 甜菊糖苷转运
BR112020023198A2 (pt) 2018-05-17 2021-02-09 Dsm Ip Assets B.V. processo para produção de um polipeptídeo
US20210115412A1 (en) * 2018-05-22 2021-04-22 Dsm Ip Assets B.V. Modified sterol acyltransferases
CN112204151B (zh) 2018-05-30 2024-06-11 维尔萨利斯股份公司 从碳水化合物材料产生糖的方法
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
CA3116984A1 (en) * 2018-10-23 2020-04-30 MARA Renewables Corporation Adaptation and process optimization of microorganisms for growth in hemicellulosic derived carbohydrates
CN109593662B (zh) * 2018-12-11 2021-12-03 大连理工大学 一种乙酸和木糖共利用的马克斯克鲁维酵母菌株及筛选方法
CN109504695B (zh) * 2019-01-02 2022-08-09 中国农业科学院作物科学研究所 多中心单鞭毛菌木糖异构酶基因及其应用
WO2020182843A1 (en) 2019-03-12 2020-09-17 Dsm Ip Assets B.V. Process for producing a fermentation broth
US20220340943A1 (en) 2019-09-10 2022-10-27 Dsm Ip Assets B.V. Enzyme composition
CN114667347A (zh) 2019-11-08 2022-06-24 帝斯曼知识产权资产管理有限公司 用于生产乙醇的方法
EP4232568A1 (en) 2020-10-22 2023-08-30 DSM IP Assets B.V. Microorganisms for diterpene production
CA3214435A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
WO2022214459A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
CN117178060A (zh) 2021-04-06 2023-12-05 帝斯曼知识产权资产管理有限公司 酶组合物
EP4320259A1 (en) 2021-04-08 2024-02-14 Versalis S.p.A. Process for the preparation of a sugar product and a fermentation product
CN115851850A (zh) * 2021-09-23 2023-03-28 中国科学院天津工业生物技术研究所 利用二氧化碳制备淀粉的方法、重组微生物和构建重组微生物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10117771A (ja) * 1996-10-23 1998-05-12 Natl Food Res Inst 冷凍生地耐性実用パン酵母
US6566107B1 (en) * 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
BRPI0306740B1 (pt) 2002-01-23 2019-08-27 C5 Yeast Company B V célula hospedeira transformada com um construto de ácido nucléico e processo para a produção de etanol
DK2338895T3 (da) * 2003-05-02 2013-09-08 Cargill Inc Genetisk modificerede gærspecies og fermenteringsproces under anvendelse af genetisk modificeret gær
BRPI0513437A (pt) * 2004-07-16 2008-05-06 Univ Delft Tech célula hospedeira eucariótica com a capacidade para isomerizar diretamente xilose em xilulose, e, processos para produzir etanol e um produto de fermentação

Also Published As

Publication number Publication date
EP1781772A1 (en) 2007-05-09
BRPI0513437A (pt) 2008-05-06
CN101914462A (zh) 2010-12-15
CA2573483A1 (en) 2006-01-26
WO2006009434A1 (en) 2006-01-26
CN101052706B (zh) 2015-02-18
US20120225451A1 (en) 2012-09-06
PL1781772T3 (pl) 2017-03-31
US20150010943A1 (en) 2015-01-08
US8790903B2 (en) 2014-07-29
JP2008506383A (ja) 2008-03-06
EP1781772B1 (en) 2016-09-28
ES2607891T3 (es) 2017-04-04
CA2573483C (en) 2014-10-28
JP2013055948A (ja) 2013-03-28
CA2861834A1 (en) 2006-01-26
JP5796925B2 (ja) 2015-10-21
CN101052706A (zh) 2007-10-10
US8034591B2 (en) 2011-10-11
CN101914462B (zh) 2013-04-24
US20080261287A1 (en) 2008-10-23

Similar Documents

Publication Publication Date Title
DK1781772T3 (da) Metabolisk modificering af xylose-fermenterende eukaryote celler
CA2664646C (en) Metabolic engineering of arabinose- fermenting yeast cells
DK1468093T4 (da) Fermentering af pentose-sukre
US20110104736A1 (en) Selection of organisms capable of fermenting mixed substrates
US20100304454A1 (en) Novel arabinose-fermenting eukaryotic cells
DK2069476T3 (da) Metabolisk modificering af arabinose-fermenterende gærceller
Winkler et al. Metabolic engineering of xylose fermenting eukaryotic cells