DK1781772T3 - Metabolisk modificering af xylose-fermenterende eukaryote celler - Google Patents

Metabolisk modificering af xylose-fermenterende eukaryote celler Download PDF

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DK1781772T3
DK1781772T3 DK05763114.5T DK05763114T DK1781772T3 DK 1781772 T3 DK1781772 T3 DK 1781772T3 DK 05763114 T DK05763114 T DK 05763114T DK 1781772 T3 DK1781772 T3 DK 1781772T3
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Aaron Adriaan Winkler
Sipko Maarten Kuyper
Laat Wilhelmus Theodorus Antonius Maria De
Dijken Johannes Pieter Van
Jacobus Thomas Pronk
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Dsm Ip Assets Bv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (21)

1. Gær eller trådformet svampeværtscelle, der er transformeret med en nukleinsyrekonstruktion, der omfatter en nukleotidsekvens, der koder for en xyloseisomerase, hvilken xyloseisomerase omfatter en aminosyresekvens, der har mindst 95 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 2, hvor nukleinsyrekonstruktionen ved transformation af værtscellen bibringer værtscellen evnen til direkte isomerisering af xylose til xylulose, hvor værtscellen omfatter en genmodifikation, der øger strømningen via pentosephosphat-reaktionsvejen, og hvor genmodifikationen omfatter overekspression af mindst ét gen i den ikke-oxidative del af pentosephosphat-reaktionsvejen sammenlignet med en værtscelle, der er genetisk identisk bortset fra genmodifikationen.
2. Værtscelle ifølge krav 1, hvor nukleotidsekvensen koder for en xyloseisomerase, der kan opnås fra en bakterie fra klassen Bacteroides.
3. Værtscelle ifølge krav 1 eller 2, hvor genet i den ikke-oxidative del af pentosephosphat-reaktionsvejen er udvalgt fra gruppen, der består af generne, der koder for ribulose-5-phosphatisomerase, ribulose-5-phosphatepimerase, transketolase og transaldolase.
4. Værtscelle ifølge krav 3, hvor genmodifikationen omfatter overekspression af mindst generne, der koder for en transketolase og en transaldolase.
5. Værtscelle ifølge et hvilket som helst af kravene 1-4, hvor værtscellen yderligere omfatter en genmodifikation, der øger den specifikke xylulosekinaseaktivitet.
6. Værtscelle ifølge krav 5, hvor genmodifikationen omfatter overekspression af et gen, der koder for en xylulosekinase.
7. Værtscelle ifølge et hvilket som helst af kravene 1-6, hvor genet, der overudtrykkes, er endogent for værtscellen.
8. Værtscelle ifølge et hvilket som helst af kravene 1-7, hvor værtscellen omfatter en genmodifikation, der reducerer uspecifik aldosereductaseaktivitet i værtscellen.
9. Værtscelle ifølge krav 8, hvor genmodifikationen reducerer ekspressionen af eller inaktiverer et gen, der koder for en uspecifik aldosereductase.
10. Værtscelle ifølge krav 9, hvor genet er inaktiveret ved deletion af mindst en del af genet eller ved destruktion af genet.
11. Værtscelle ifølge krav 8 eller 9, hvor ekspressionen af hvert gen i værtscellen, der koder for en uspecifik aldosereductase, er reduceret eller inaktiveret.
12. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen er en gær, fortrinsvis en gær, der tilhører en af slægterne: Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces og Yarrowia.
13. Værtscelle ifølge krav 12, hvor gæren tilhører en af arterne: S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, K. lactis, K. marxianus og K. fragilis.
14. Værtscelle ifølge et hvilket som helst af kravene 1-11, hvor værtscellen er en trådformet svamp, fortrinsvis en trådformet svamp, der tilhører en af slægterne: Aspergillus, Trichoderma, Humicola, Acremonium, Fusarium og Penicillium.
15. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen udtrykker et eller flere enzymer, der bibringer værtscellen evnen til at danne mindst ét fermenteringsprodukt udvalgt fra gruppen, der består af ethanol, mælkesyre, 3-hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner.
16. Fremgangsmåde til fremstillng af ethanol, hvilken fremgangsmåde omfatter trinene: (a) fermentering af et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge et hvilket som helst af kravene 1-14, hvor værtscellen fermenterer xylose til ethanol, og eventuelt (b) indvinding af ethanol.
17. Fremgangsmåde ifølge krav 16, hvor mediet også indeholder en kilde af glucose.
18. Fremgangsmåde ifølge krav 16 eller 17, hvor den volumetriske produktivitet af ethanol er mindst 0,5 g ethanol pr. liter pr. time.
19. Fremgangsmåde ifølge et hvilket som helst af kravene 16-18, hvor ethanoludbyttet er mindst 50 %.
20. Fremgangsmåde til fremstilling af et fermenteringsprodukt udvalgt fra gruppen, der består af mælkesyre, 3- hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner, hvilken fremgangsmåde omfatter trinene: (a) fermentering at et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge krav 15, hvor værtscellen fermenterer xylose til fermenteringsproduktet, og eventuelt (b) indvinding af fermenteringproduktet.
21. Fremgangsmåde ifølge krav 20, hvor mediet også indeholder en kilde af glucose.
DK05763114.5T 2004-07-16 2005-07-15 Metabolisk modificering af xylose-fermenterende eukaryote celler DK1781772T3 (da)

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US58838104P 2004-07-16 2004-07-16
EP04077073 2004-07-16
US58983304P 2004-07-22 2004-07-22
PCT/NL2005/000516 WO2006009434A1 (en) 2004-07-16 2005-07-15 Metabolic engineering of xylose fermenting eukaryotic cells

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EP (1) EP1781772B1 (da)
JP (2) JP5796925B2 (da)
CN (2) CN101914462B (da)
BR (1) BRPI0513437A (da)
CA (2) CA2573483C (da)
DK (1) DK1781772T3 (da)
ES (1) ES2607891T3 (da)
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WO (1) WO2006009434A1 (da)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0306740A (pt) 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
CN101914462B (zh) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程
EP1873248A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a glucose-6-phosphate isomerase
EP1873246A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a transaldolase
AU2007302867B2 (en) * 2006-10-02 2013-11-07 Dsm Ip Assets B.V. Metabolic engineering of arabinose-fermenting yeast cells
PL2069476T3 (pl) * 2006-10-02 2017-02-28 Dsm Ip Assets B.V. Metaboliczne opracowywanie komórek drożdży fermentujących arabinozę
WO2008144012A2 (en) * 2007-05-14 2008-11-27 The Regents Of The University Of California Xylose- and arabinose- metabolizing enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same
US20100304454A1 (en) * 2007-07-19 2010-12-02 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
EP2062967A1 (en) 2007-11-21 2009-05-27 Technical University of Denmark Genetically engineered aspergillus
WO2009109634A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
AU2009221104B2 (en) * 2008-03-07 2014-07-31 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109630A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109631A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
GB0812318D0 (en) 2008-07-04 2008-08-13 Terranol As Microorganism
WO2010014817A2 (en) * 2008-07-30 2010-02-04 Novozymes A/S Producing fermentation products
CN108315272A (zh) * 2008-11-21 2018-07-24 拉勒曼德匈牙利流动性管理有限责任公司 用于使用纤维素进行同时糖化和发酵的表达纤维素酶的酵母
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
CN102307987B (zh) * 2008-12-24 2016-11-16 帝斯曼知识产权资产管理有限公司 木糖异构酶基因及其在戊糖发酵中的用途
AU2010216616A1 (en) * 2009-02-23 2011-10-20 Kirin Holdings Kabushiki Kaisha Manufacturing method for substances from Candida utilis that can use xylose as carbon source
CA2753538A1 (en) 2009-02-25 2010-09-02 Indiana University Research & Technology Corporation Materials and methods for identifying and using yeast strains that metabolize pentose sugars in the presence of d-glucose
WO2010147642A1 (en) 2009-06-15 2010-12-23 Massachusetts Institute Of Technology Production of triacylglycerides, fatty acids, and their derivatives
EP2451960A2 (en) * 2009-07-09 2012-05-16 Verdezyne, Inc. Engineered microorganisms with enhanced fermentation activity
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
JP5804327B2 (ja) * 2009-11-30 2015-11-04 国立大学法人神戸大学 バイオマスからのエタノールの生産方法
WO2011078262A1 (ja) * 2009-12-22 2011-06-30 株式会社豊田中央研究所 キシロースイソメラーゼ及びその利用
MX2012012171A (es) 2010-04-21 2013-02-27 Dsm Ip Assets Bv Proceso para la produccion de celulas que tienen la capacidad de convertir arabinosa.
BR112012028290B1 (pt) 2010-05-05 2021-02-02 Lallemand Hungary Liquidity Management Llc. levedura recombinante, processo para converter biomassa em etanol e meio de fermentação compreendendo dita levedura
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
BR112012031362A2 (pt) 2010-06-03 2015-10-13 Mascoma Corp levedura expressando enzimas sacarolíticas para bioprocessamento consolidado usando amido e celulose
US8679822B2 (en) * 2010-06-29 2014-03-25 E I Du Pont De Nemours And Company Xylose utilization in recombinant zymomonas
WO2012019175A2 (en) 2010-08-06 2012-02-09 Mascoma Corporation Production of malonyl-coa drived products via anaerobic pathways
CN102399804A (zh) * 2010-09-15 2012-04-04 中国农业科学院作物科学研究所 木糖异构酶基因的功能及其应用
PL2627765T3 (pl) 2010-10-13 2017-10-31 Dsm Ip Assets Bv Komórka drożdżowa fermentująca pentozę i glukozę
PL2663645T3 (pl) 2010-11-18 2015-05-29 Dsm Ip Assets Bv Szczepy drożdży zmodyfikowane do wytwarzania etanolu z glicerolu
JP2012120491A (ja) * 2010-12-09 2012-06-28 Toyota Motor Corp キシロースを含む培地における発酵培養方法
WO2012088467A2 (en) 2010-12-22 2012-06-28 Mascoma Corporation Genetically modified clostridium thermocellum engineered to ferment xylose
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
BR122019017739B1 (pt) 2011-04-05 2021-06-22 Lallemand Hungary Liquidity Management Llc Micro-organismo recombinante compreendendo uma deleção de enzimas nativas que atuam para produzir glicerol e/ou regular a síntese de glicerol e vias metabólicas sintéticas para converter uma fonte de carboidrato a etanol
CA2833312A1 (en) 2011-04-22 2012-10-26 Dsm Ip Assets B.V. Yeast cell capable of converting sugars including arabinose and xylose
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
CN104126011B (zh) 2011-11-30 2017-07-11 帝斯曼知识产权资产管理有限公司 由乙酸和甘油生产乙醇的工程化酵母菌株
CN104203005A (zh) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
WO2013134424A1 (en) * 2012-03-06 2013-09-12 Lygos, Inc. Recombinant host cells for the production of malonate
JP6249391B2 (ja) * 2012-06-15 2017-12-20 国立研究開発法人農業・食品産業技術総合研究機構 キシロースを高温で発酵する方法
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
IN2015DN01041A (da) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
WO2014035458A1 (en) 2012-08-29 2014-03-06 Mascoma Corporation Expression of enzymes in yeast for lignocellulose derived oligomer cbp
US10604773B2 (en) 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
AR093025A1 (es) 2012-10-16 2015-05-13 Dsm Ip Assets Bv Celulas con conversion mejorada de pentosas
US20150252319A1 (en) 2012-11-07 2015-09-10 Dsm Ip Assets B.V. pH CONTROLLED YEAST PROPAGATION
CA2889890C (en) 2012-11-09 2023-03-28 Lallemand Hungary Liquidity Management Llc Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014160402A1 (en) 2013-03-14 2014-10-02 Mascoma Corporation Co-conversion of carbohydrates to fermentation products in a single fermentation step
DE102013205986A1 (de) 2013-04-04 2014-10-23 Wacker Chemie Ag Mikroorganismenstamm und Verfahren zur fermentativen Herstellung von C4-Verbindungen aus C5-Zuckern
DK3004366T3 (da) 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
CA2914900A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Recombinant microorganism and process for the production of steviol glycosides
CN108064226A (zh) 2013-07-31 2018-05-22 帝斯曼知识产权资产管理有限公司 甜菊糖苷的回收
EP3033413B2 (en) 2013-08-15 2023-05-10 Lallemand Hungary Liquidity Management LLC Methods for the improvement of product yield and production in a microorganism through glycerol recycling
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
WO2015066642A1 (en) * 2013-11-04 2015-05-07 The Board Of Trustees Of The Leland Stanford Junior University Benzylisoquinoline alkaloid (bia) precursor producing microbes, and methods of making and using the same
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
BR102014014407A2 (pt) * 2014-06-12 2016-04-19 Biocelere Agroindustrial Ltda cassete de expressão para transformar célula eucariótica, micro-organismo geneticamente modificado com eficiente consumo de xilose, processo para produção de biocombustíveis e bioquímicos e biocombustível e/ou bioquímico assim produzido
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
BR102014027233A2 (pt) * 2014-10-30 2016-05-24 Biocelere Agroindustrial Ltda cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos
DK3640336T3 (da) 2015-01-28 2022-06-27 Dsm Ip Assets Bv Fremgangsmåde til enzymatisk hydrolyse af lignocellulosemateriale og fermentering af sukre
CA2973302C (en) 2015-01-28 2022-10-25 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
EP3274448B1 (en) 2015-03-23 2021-08-11 DSM IP Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
CA2980090A1 (en) 2015-04-03 2016-10-06 Dsm Ip Assets B.V. Steviol glycosides
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
CN107532135A (zh) 2015-04-21 2018-01-02 帝斯曼知识产权资产管理有限公司 萜类化合物的微生物生产
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
MY192153A (en) 2015-07-10 2022-08-02 Dsm Ip Assets Bv Steviol glycoside composition
MX2018000502A (es) 2015-07-13 2018-09-07 Mara Renewables Corp Mejora del metabolismo de microalga de xilosa.
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
CA2993744A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
CN106554924B (zh) * 2015-09-24 2019-12-06 中粮营养健康研究院有限公司 生产乙醇的重组酿酒酵母菌株、其构建方法以及利用该菌株生产乙醇的方法
BR112018006724A2 (pt) 2015-10-05 2018-10-09 Dsm Ip Assets Bv ácido caurenoico hidroxilases
BR122023025807A2 (pt) * 2015-11-06 2024-01-16 Lallemand Hungary Liquidity Management Llc Célula hospedeira de levedura recombinante e método para aumentar a produção de um produto de fermentação
CN105838744A (zh) * 2016-04-26 2016-08-10 上海交通大学 制备2-表-5-表有效醇酮的方法
EP3469090A1 (en) 2016-06-09 2019-04-17 DSM IP Assets B.V. Seed train for large scale enzyme production
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
EP3497109A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
CN109563117A (zh) 2016-08-09 2019-04-02 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷的结晶
AU2017350309A1 (en) 2016-10-27 2019-05-02 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
CA3040380A1 (en) 2016-11-04 2018-05-11 Inbicon A/S Method for preparing fermentable sugars from lignocellulosic biomass
BR112019010355B1 (pt) 2016-11-24 2024-03-05 Dsm Ip Assets B.V. Composição de enzimas
CA3043435A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme compositions with improved hydrolysis performance
WO2018104238A1 (en) 2016-12-08 2018-06-14 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
BR112019012949A2 (pt) 2016-12-21 2019-11-26 Globalyeast N V isomerases de xilose que conferem capacidade de fermentação eficiente de xilose em levedura
CA3047889A1 (en) * 2016-12-22 2018-06-28 Lallemand Hungary Liquidity Management Llc Sulfite tolerance in recombinant yeast host cells
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP4155400A1 (en) 2017-06-27 2023-03-29 DSM IP Assets B.V. Udp-glycosyltransferases
WO2019046043A1 (en) * 2017-08-29 2019-03-07 Danisco Us Inc MODIFIED YEAST COMPRISING GLUCOSE-SPECIFIC ATP MEDIATION TRANSPORTERS
BR112020005439A2 (pt) 2017-10-09 2020-09-24 Dsm Ip Assets B.V. processo para hidrólise enzimática de material lignocelulósico e fermentação de açúcares
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3704259A1 (en) 2017-10-30 2020-09-09 DSM IP Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3775189A1 (en) 2018-03-28 2021-02-17 DSM IP Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
WO2019211230A1 (en) 2018-04-30 2019-11-07 Dsm Ip Assets B.V. Steviol glycoside transport
WO2019219804A1 (en) 2018-05-17 2019-11-21 Dsm Ip Assets B.V. Process for producing a polypeptide
US20210115412A1 (en) * 2018-05-22 2021-04-22 Dsm Ip Assets B.V. Modified sterol acyltransferases
LT3802843T (lt) 2018-05-30 2023-04-25 Versalis S.P.A. Cukrų gamybos iš angliavandenių medžiagos būdas
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
CA3116984A1 (en) * 2018-10-23 2020-04-30 MARA Renewables Corporation Adaptation and process optimization of microorganisms for growth in hemicellulosic derived carbohydrates
CN109593662B (zh) * 2018-12-11 2021-12-03 大连理工大学 一种乙酸和木糖共利用的马克斯克鲁维酵母菌株及筛选方法
CN109504695B (zh) * 2019-01-02 2022-08-09 中国农业科学院作物科学研究所 多中心单鞭毛菌木糖异构酶基因及其应用
EP3938525A1 (en) 2019-03-12 2022-01-19 DSM IP Assets B.V. Process for producing a fermentation broth
US20220340943A1 (en) 2019-09-10 2022-10-27 Dsm Ip Assets B.V. Enzyme composition
WO2021089877A1 (en) 2019-11-08 2021-05-14 Dsm Ip Assets B.V. Process for producing ethanol
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
US20240182936A1 (en) 2021-04-06 2024-06-06 Dsm Ip Assets B.V. Enzyme composition
CA3213845A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
BR112023020357A2 (pt) 2021-04-06 2023-11-21 Dsm Ip Assets Bv Composição de enzima
WO2022214460A1 (en) 2021-04-08 2022-10-13 Dsm Ip Assets B.V. Process for the preparation of a sugar product and a fermentation product
CN115851850A (zh) * 2021-09-23 2023-03-28 中国科学院天津工业生物技术研究所 利用二氧化碳制备淀粉的方法、重组微生物和构建重组微生物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10117771A (ja) * 1996-10-23 1998-05-12 Natl Food Res Inst 冷凍生地耐性実用パン酵母
US6566107B1 (en) * 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
BR0306740A (pt) * 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
CN1922198B (zh) * 2003-05-02 2012-07-11 卡吉尔公司 基因修饰酵母物种和使用基因修饰酵母的发酵方法
CN101914462B (zh) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程

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