DK1468093T4 - Fermentering af pentose-sukre - Google Patents

Fermentering af pentose-sukre Download PDF

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DK1468093T4
DK1468093T4 DK03731853.2T DK03731853T DK1468093T4 DK 1468093 T4 DK1468093 T4 DK 1468093T4 DK 03731853 T DK03731853 T DK 03731853T DK 1468093 T4 DK1468093 T4 DK 1468093T4
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host cell
xylose
ala
ethanol
nucleic acid
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DK1468093T3 (da
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Den Camp Hubertus Johannes Marie Op
Harry Ramanoedj Harhangi
Der Drift Christiaan Van
Jacobus Thomas Pronk
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Dsm Ip Assets Bv
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    • C12Y503/01005—Xylose isomerase (5.3.1.5)
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Claims (17)

1. Gær- eller filamentøs svampe-værtscelle transformeret med et nukleinsy-rekonstrukt omfattende en nukleotidsekvens, der koder xylose isomerase omfattende en aminosyresekvens, som har mindst 70 % sekvensidentitet med aminosyresekvensen SEQ ID NO: 1, bestemt med anvendelse af Need-leman og Wunsch-algoritme, BLOSSUM 62-sammenligningsmatrix, en Gap penalty på 12 og en Gap-længde-penalty på 4, idet nukleinsyrekonstruktet, efter transformation af værtscellen, giver værtscellen evnen til at isomerise xylose til xylulose, hvilket giver værtscellen evnen til at vokse på xylose som carbonkilde.
2. Transformeret værtscelle ifølge krav 1, hvor værtscellen er gær, som tilhører en af slægterne: Saccharomyces, Kluyveromyces, Candida, Pichia, Schi-zosaccharomyces, Hansenula, Kloeckera, Schwanniomyces og Yarrowia.
3. Transformeret værtscelle ifølge krav 2, hvor gæret tilhører en af følgende arter: S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastati-cus, K. lactis, K. marxianus og K. fragilis.
4. Transformeret værtscelle ifølge krav 1, hvor værtscellen er en trådagtig svamp, som tilhører en af følgende slægter: Aspergillus, Trichoderma, Humi-cola, Acremonium, Fusarium og Penicillium.
5. Transformeret værtscelle ifølge et hvilket som helst af de foregående krav, hvor nukleotidsekvensen, der koder en xylose-isomerase, er operabelt koblet til en promoter, som forårsager tilstrækkelig ekspression af xylose-isomerasen i værtscellen til at give værtscellen evnen til at isomerisere xylose til xylulose.
6. Transformeret værtscelle ifølge krav 5, hvor promoteren ikke er følsom over for katabolisk repression i værtscellen.
7. Transformeret værtscelle ifølge et hvilket som helst af de foregående krav, hvor værtscellen omfatter en genetisk modifikation, som resulterer i et kendetegn valgt fra gruppen bestående af: (a) forøget transport af xylose ind i værtscellen; (b) forøget xylulose-kinaseaktivitet; (c) forøget flux af pentose phosphat-pathway; (d) nedsat følsomhed over for katabolisk repression; (e) forøget tolerance over for ethanol, osmolaritet eller organiske syrer; og (f) reduceret produktion af biprodukter.
8. Transformeret værtscelle ifølge krav 7, hvor den genetiske modifikation består af overekspression af et endogent gen, ekspression af et heterologt gen, eller en kombination deraf, og hvor det endogene, eller heterologe gen er valgt fra gruppen bestående af et gen, som koder: en hexose- eller pento-se-transporter, en xylulose-kinase; et enzym fra pentose-phosphat-pathway, et glycolytisk enzym og et ethanologent enzym.
9. Transformeret værtscelle ifølge krav 7, hvor den genetiske modifikation består i inaktivering af et endogent gen, hvor genet er valgt fra gruppen bestående af et hexose-kinase-gen, Saccharomyces MIG1 og MIG2 gener og hybridiserende homologer deraf.
10. Transformeret værtscelle ifølge et hvilket som helst af de foregående krav, hvor værtscellen udtrykker et eller flere enzymer, som giver værtscellen evnen til at producere mælkesyre, eddikesyre, raysyre, aminosyrer, 1,3-propan-diol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner.
11. Transformeret værtscelle ifølge krav 10, hvor værtscellen indeholder en genetisk modifikation, som resulterer i nedsat alkohol-dehydrogenaseaktivitet.
12. Fremgangsmåde til fremstilling af ethanol, hvor fremgangsmåden omfatter trinnene: (a) at fermentere et medium indeholdende en kilde af xylose med en transformeret værtscelle som defineret i et hvilket som helst af kravene 1 - 9, hvorved værtscellen fermenterer xylose til ethanol, og eventuelt (b) genvinde ethanolen.
13. Fremgangsmåde ifølge krav 12, hvor mediet også indeholder en glucose-kilde.
14. Fremgangsmåde ifølge krav 12 eller 13, hvor den volumetriske ethanol-produktivitet er mindst 0,5 g ethanol pr. liter pr. time.
15. Fremgangsmåde ifølge et hvilket som helst af kravene 12 - 14, hvor ethanoludbyttet er mindst 50 % af det teoretiske udbytte på 0,51 g ethanol pr. g glucose eller xylose.
16. Fremgangsmåde til fremstilling af et fermenteringsprodukt valgt fra gruppen bestående af mælkesyre, eddikesyre, ravsyre, aminosyrer, 1,3-propan-diol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner, hvor fremgangsmåden omfatter trinnene: (a) at fermentere et medium indeholdende en kilde af xylose med en transformeret værtscelle som defineret i krav 10 eller 11, hvorved værtscellen fermenterer xylose til fermenteringsproduktet, og eventuelt (b) genvinde fermenteringsproduktet.
17. Fremgangsmåde ifølge krav 16, hvor mediet også indeholder en glucose-kilde.
DK03731853.2T 2002-01-23 2003-01-23 Fermentering af pentose-sukre DK1468093T4 (da)

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EP02075266 2002-01-23
PCT/NL2003/000049 WO2003062430A1 (en) 2002-01-23 2003-01-23 Fermentation of pentose sugars

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DK1468093T3 DK1468093T3 (da) 2009-02-16
DK1468093T4 true DK1468093T4 (da) 2018-04-09

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US (6) US7622284B2 (da)
EP (1) EP1468093B2 (da)
JP (4) JP4334352B2 (da)
CN (1) CN100448996C (da)
AT (1) ATE418616T2 (da)
BR (2) BRPI0306740B1 (da)
CA (1) CA2474033C (da)
CY (1) CY1108905T1 (da)
DE (1) DE60325457D1 (da)
DK (1) DK1468093T4 (da)
ES (1) ES2319757T5 (da)
PT (1) PT1468093E (da)
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WO (1) WO2003062430A1 (da)

Families Citing this family (289)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1468093B2 (en) * 2002-01-23 2018-01-24 DSM IP Assets B.V. Fermentation of pentose sugars
US20050233030A1 (en) 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
CN102210376B (zh) 2003-03-10 2014-12-31 波伊特研究股份有限公司 利用生淀粉生产乙醇的方法
ES2543385T3 (es) 2003-05-02 2015-08-18 Cargill, Incorporated Especies de levadura genéticamente modificadas y procesos de fermentación que emplean levaduras genéticamente modificadas
US20080032373A1 (en) * 2004-05-13 2008-02-07 Novozymes North America, Inc. Process Of Producing A Fermentation Product
CN101052706B (zh) * 2004-07-16 2015-02-18 帝斯曼知识产权资产管理有限公司 发酵木糖的真核细胞的代谢工程
US20150328347A1 (en) 2005-03-24 2015-11-19 Xyleco, Inc. Fibrous materials and composites
US7708214B2 (en) 2005-08-24 2010-05-04 Xyleco, Inc. Fibrous materials and composites
KR20080028902A (ko) 2005-06-02 2008-04-02 카아길, 인코포레이팃드 이싸첸키아 오리엔탈리스에 속하는 종 및 그와 밀접한관련이 있는 종의 유전자 변형된 효모 및 이를 이용한 발효방법
PT103331A (pt) * 2005-08-05 2007-02-28 Fundacao Da Faculdade De Cienc Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente
US7919289B2 (en) 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
US7357527B2 (en) * 2006-03-03 2008-04-15 Thomas A Meyers Solar sign light
JP5507045B2 (ja) 2006-12-15 2014-05-28 石原産業株式会社 アントラニルアミド系化合物の製造方法
DE102006060381B4 (de) * 2006-12-20 2010-04-08 Johann Wolfgang Goethe-Universität Frankfurt am Main Neuer spezifischer Arabinose-Transporter aus der Hefe Pichia stipitis und dessen Verwendungen
CN101765661B (zh) * 2007-06-01 2014-08-06 索拉兹米公司 在微生物中生产油
CA2693365A1 (en) * 2007-07-19 2009-01-22 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
EP2062967A1 (en) 2007-11-21 2009-05-27 Technical University of Denmark Genetically engineered aspergillus
EP2128262A1 (en) * 2008-05-28 2009-12-02 Università Degli Studi Di Milano - Bicocca Improved yeast strains for organic acid production
DE102008031350B4 (de) * 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
GB0812318D0 (en) 2008-07-04 2008-08-13 Terranol As Microorganism
US8440449B2 (en) 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
CN107034142A (zh) 2008-11-28 2017-08-11 泰拉瑞亚控股公司 在异养型微生物中制备特制油
EP2373788A1 (en) 2008-12-04 2011-10-12 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
GB0822937D0 (en) * 2008-12-16 2009-01-21 Terranol As Microorganism
CA2747056A1 (en) 2008-12-19 2010-07-15 Novozymes, Inc. Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
EP3141609A1 (en) 2008-12-19 2017-03-15 Novozymes, Inc. Methods for increasing hydrolysis of cellulosic material in the presence of cellobiose dehydrogenase
US8337663B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for increasing hydrolysis of cellulosic material
JP5821132B2 (ja) 2008-12-24 2015-11-24 ディーエスエム アイピー アセッツ ビー．ブイ． キシロース異性化酵素遺伝子及びペントース糖の発酵におけるそれらの使用
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2010088463A2 (en) 2009-01-30 2010-08-05 Novozymes, Inc. Polypeptides having expansin activity and polynucleotides encoding same
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
US8815552B2 (en) 2009-03-03 2014-08-26 Poet Research, Inc. System for fermentation of biomass for the production of ethanol
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
JP5608999B2 (ja) * 2009-04-08 2014-10-22 株式会社豊田中央研究所 キシロースを利用して有用物質を生産する方法
MX2011012585A (es) 2009-05-29 2011-12-14 Novozymes Inc Metodos para incrementar la degradacion o conversion de material celulosico.
CA2764148A1 (en) 2009-06-02 2010-12-09 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2011005867A1 (en) 2009-07-07 2011-01-13 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity activity and polynucleotides encoding same
IN2012DN00568A (da) 2009-07-09 2015-06-12 Verdzyne Inc
WO2011035027A2 (en) 2009-09-17 2011-03-24 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035029A1 (en) 2009-09-18 2011-03-24 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
CN102648276A (zh) 2009-09-29 2012-08-22 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
CN102597228A (zh) 2009-10-23 2012-07-18 诺维信股份有限公司 纤维二糖水解酶变体及编码其的多核苷酸
BR112012006847A2 (pt) 2009-10-29 2015-09-08 Novozymes As polipeptídeo polinucleotídeo, célula hospedeira recombinante, métodos para produzir o polipeptídeo para produzir um mutante de uma célula parental, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para produzir um produto de fermentação e pra fermentar um material celulósico, planta transgênica, parte da planta ou célula da planta, molécula de rna inibitória de filamento duplo, e, composição.
DK2496693T3 (da) 2009-11-06 2018-01-22 Novozymes Inc Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
EP2496692B1 (en) 2009-11-06 2016-03-16 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
CA2780179A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Compositions for saccharification of cellulosic material
WO2011059314A1 (en) * 2009-11-12 2011-05-19 Stichting Voor De Technische Wetenschappen Pentose transporters and uses thereof
CA2785276C (en) 2009-12-22 2019-04-23 Kabushiki Kaisha Toyota Chuo Kenkyusho Xylose isomerase and use thereof
ES2651313T3 (es) 2009-12-28 2018-01-25 Dsm Ip Assets B.V. Traustoquítridos recombinantes que crecen en xilosa, y composiciones, métodos de preparación y usos de los mismos
CN102947440B (zh) 2009-12-30 2015-04-08 埃欧金能源公司 呈现增强的木质纤维素水解产物发酵的改良酵母菌株
US8759049B2 (en) 2010-02-25 2014-06-24 Iogen Energy Corporation Method for the production of a fermentation product from a sugar hydrolysate
ES2651076T3 (es) * 2010-04-21 2018-01-24 Dsm Ip Assets B.V. Célula adecuada para la fermentación de una composición de azúcares mixtos
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
SG185780A1 (en) 2010-05-28 2013-01-30 Solazyme Inc Tailored oils produced from recombinant heterotrophic microorganisms
CA2798937A1 (en) * 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
EP3168300A1 (en) 2010-06-03 2017-05-17 Lallemand Hungary Liquidity Management LLC Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
WO2011159915A1 (en) 2010-06-17 2011-12-22 Poet Research, Inc. Fermentation of biomass
CN103108951A (zh) 2010-06-30 2013-05-15 诺维信公司 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸
FI2591119T4 (fi) 2010-07-07 2023-01-13 Fermentointimenetelmä GH61-polypeptidien kanssa
US20130189747A1 (en) 2010-08-12 2013-07-25 Novozymes, Inc. Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Sulfur-Containing Compound And Uses Thereof
WO2012024046A2 (en) * 2010-08-20 2012-02-23 Codexis, Inc. Pentose fermentation by a recombinant microorganism
DK2606131T3 (da) 2010-08-20 2017-06-19 Codexis Inc Anvendelse af proteiner fra glycosidhydrolase 61-familie til forarbejdning af cellulose
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US20130212746A1 (en) 2010-08-30 2013-08-15 Novoyzmes A/S Polypeptides Having Hemicellulolytic Activity And Polynucleotides Encoding Same
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2611901B1 (en) 2010-08-30 2016-05-11 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
WO2012030799A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012030849A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
US8709770B2 (en) 2010-08-31 2014-04-29 Iogen Energy Corporation Process for improving the hydrolysis of cellulose in high consistency systems using one or more unmixed and mixed hydrolysis reactors
CN102399804A (zh) * 2010-09-15 2012-04-04 中国农业科学院作物科学研究所 木糖异构酶基因的功能及其应用
EP2622068B1 (en) 2010-09-30 2016-07-20 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN107345226A (zh) 2010-09-30 2017-11-14 诺维信股份有限公司 具有纤维素分解增强活性的多肽变体及其编码多核苷酸
EP2622069B1 (en) 2010-10-01 2015-11-25 Novozymes, Inc. Beta-glucosidase variants and polynucleotides encoding same
KR20130100169A (ko) * 2010-10-13 2013-09-09 디에스엠 아이피 어셋츠 비.브이. 펜토스 및 글루코스 발효 효모 세포
WO2012058293A1 (en) 2010-10-26 2012-05-03 Novozymes North America, Inc. Methods of saccharifying sugarcane trash
ES2541492T3 (es) 2010-11-02 2015-07-21 Novozymes, Inc. Métodos para pretratar material celulósico con polipéptido GH61
JP5970463B2 (ja) 2010-11-03 2016-08-17 ソラザイム, インコーポレイテッドSolazyme Inc 流動点が低い微生物油、それから生成される誘電性流体、及び関連する方法
WO2012059053A1 (en) 2010-11-04 2012-05-10 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012063344A1 (ja) * 2010-11-11 2012-05-18 トヨタ自動車株式会社 組換え酵母を用いたエタノールの製造方法
DK2638153T3 (da) 2010-11-12 2017-10-16 Novozymes Inc Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem
EP2663645B1 (en) 2010-11-18 2014-12-17 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from glycerol
US9676830B2 (en) 2010-11-18 2017-06-13 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8927235B2 (en) 2010-12-06 2015-01-06 Novozymes A/S Methods of hydrolyzing oligomers in hemicellulosic liquor
WO2012100187A1 (en) 2011-01-21 2012-07-26 Poet Research, Inc. Systems and methods for improving fermentation
US9080161B2 (en) 2011-01-26 2015-07-14 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012101206A2 (en) 2011-01-26 2012-08-02 Novozymes A/S Novel glycoside hydrolases from thermophilic fungi
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
MX337942B (es) 2011-01-26 2016-03-29 Novozymes As Polipeptidos que tienen actividad de endoglucanasa y polinucleotidos que codifican los mismos.
US8759023B2 (en) 2011-01-26 2014-06-24 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK2670853T3 (da) 2011-01-31 2017-08-28 Novozymes North America Inc Fremgangsmåde til enzymatisk raffinering af forbehandlet celluloseholdigt materiale til forsukring
SG192594A1 (en) 2011-02-02 2013-09-30 Solazyme Inc Tailored oils produced from recombinant oleaginous microorganisms
CN102643845A (zh) * 2011-02-21 2012-08-22 中国农业科学院作物科学研究所 大麦木糖异构酶基因的功能及其应用
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
EP2678352B1 (en) 2011-02-23 2017-12-06 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103608461B (zh) 2011-03-09 2016-08-03 诺维信公司 增加多肽的纤维素分解增强活性的方法
WO2012122477A1 (en) 2011-03-10 2012-09-13 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
EP3333258A3 (en) 2011-03-25 2018-07-25 Novozymes A/S Method for degrading or converting cellulosic material
JP5813977B2 (ja) 2011-03-30 2015-11-17 トヨタ自動車株式会社 Ｋｌｕｙｖｅｒｏｍｙｃｅｓ属の変異体酵母及びこれを用いたエタノールの製造方法
US20140080182A1 (en) 2011-03-31 2014-03-20 Novozymes, Inc. Cellulose Binding Domain Variants and Polynucleotides Encoding Same
US9410136B2 (en) 2011-03-31 2016-08-09 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
DK2702153T3 (da) 2011-04-28 2019-03-18 Novozymes Inc Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem
BR112013027463A2 (pt) 2011-04-29 2017-09-26 Novozymes Inc métodos para degradar ou converter, e para fermentar um material celulóliso, para produzir um produto de fermentação, e para limpar ou lavar uma superfície dura ou roupa suja, e, composição de detergente
EP2705138B1 (en) * 2011-05-06 2017-03-22 TerraVia Holdings, Inc. Genetically engineered microorganisms that metabolize xylose
EP2710132A1 (en) 2011-05-19 2014-03-26 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
EP2710133A1 (en) 2011-05-19 2014-03-26 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
AU2012274080B2 (en) * 2011-06-21 2016-07-28 Katholieke Universiteit Leuven, K.U.Leuven R&D Specific alleles important for ethanol tolerance
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
DK2734633T3 (da) 2011-07-22 2019-06-11 Novozymes North America Inc Fremgangsmåder til forbehandling af celluloseholdigt materiale og forbedring af hydrolyse deraf
WO2013019780A2 (en) 2011-08-04 2013-02-07 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
FI3091073T4 (fi) 2011-08-04 2023-05-11 Ksylanaasiaktiivisuutta omaavia polypeptidejä ja niitä koodaavia polynukleotideja
WO2013028701A1 (en) 2011-08-22 2013-02-28 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
CN103890165A (zh) 2011-08-24 2014-06-25 诺维信股份有限公司 纤维素分解酶组合物及其用途
US9663772B2 (en) 2011-08-24 2017-05-30 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
CN103930555A (zh) 2011-09-13 2014-07-16 诺维信北美公司 水解和发酵纤维素材料的方法
US20140308705A1 (en) 2011-09-20 2014-10-16 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
CN108373498A (zh) 2011-09-23 2018-08-07 诺维信公司 纤维素分解酶组合物及其用途
WO2013089889A2 (en) 2011-09-30 2013-06-20 Novozymes, Inc. Chimeric polypeptides having beta-glucosidase activity and polynucleotides encoding same
DK2773656T3 (da) 2011-10-31 2019-09-09 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
EP3382017A1 (en) 2011-11-18 2018-10-03 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
EP3597736A1 (en) 2011-11-21 2020-01-22 Novozymes A/S Gh61 polypeptide variants and polynucleotides encoding same
BR112014011387A2 (pt) 2011-11-22 2017-05-02 Novozymes Inc polipeptídeo e polinucleotídeo isolados, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, e uma proteína, e para inibir a expressão de um polipeptídeo, planta transgênica, parte da planta ou célula de planta, molécula de rna, processos para degradar ou converter um material celulósico ou contendo xilano, para sintetizar um produto de fermentação, e para fermentar um material celulósico ou contendo xilano, e, formulação de caldo completo ou composição de cultura de célula
BR112014012417A2 (pt) 2011-12-01 2017-06-06 Novozymes Inc polipeptídeo e polinucleotídeo isolados, célula hospedeira recombinante, métodos para a produção de um polipeptídeo, de um mutante de uma célula de origem, e de uma proteína, e para a inibição da expressão de um polipeptídeo, planta transgênica, parte da planta ou célula de planta, molécula de rna, processos para degradar ou converter um material celulósico ou contendo xilano, para produzir um produto de fermentação, e de fermentação de um material celulósico ou contendo xilano, e, formulação de caldo integral ou composição de cultura de células
EP2791330B1 (en) 2011-12-16 2017-07-26 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
EP2794870A4 (en) 2011-12-19 2015-06-17 Novozymes Inc POLYPEPTIDES WITH XYLANASE ACTIVITY AND POLYNUCLEOTIDES THAT CODE
US10036050B2 (en) 2011-12-20 2018-07-31 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US20150017670A1 (en) 2011-12-21 2015-01-15 Novozymes, Inc. Methods For Determining The Degradation Of A Biomass Material
EA201400836A8 (ru) 2012-01-23 2016-03-31 ДСМ АйПи АССЕТС Б.В. Получение дитерпена
EP2626423A1 (en) * 2012-02-07 2013-08-14 Clariant Produkte (Deutschland) GmbH Pentose-fermenting microorganism
EP2831255A2 (en) 2012-03-26 2015-02-04 Novozymes North America, Inc. Methods of preconditioning cellulosic material
EP2859110B1 (en) 2012-03-30 2022-10-26 Novozymes North America, Inc. Processes of producing fermentation products
SG11201406711TA (en) 2012-04-18 2014-11-27 Solazyme Inc Tailored oils
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
WO2013160248A2 (en) 2012-04-23 2013-10-31 Novozymes A/S Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
EP2841570B1 (en) 2012-04-23 2017-12-20 Novozymes A/S Polypeptides having glucuronyl esterase activity and polynucleotides encoding same
NL2008682C2 (en) 2012-04-23 2013-10-31 Stichting Energie Wet biomass treatment.
BR112014026625A2 (pt) 2012-04-27 2017-07-18 Novozymes Inc E Novozymes As variante do polipeptídeo, polinucleotídeo isolado, célula hospedeira recombinante, métodos para produção de uma variante do polipeptídeo e para obtenção de uma variante do polipeptídeo, planta, parte de planta ou célula de planta transgênica, processos para degradação ou conversão de um material celulósico, para produção de um produto da fermentação e para fermentação de um material celulósico, composição, e, formulação ou composição de cultura celular de caldo inteiro
CA2879680A1 (en) 2012-07-24 2014-01-30 Bp Corporation North America Inc. Xylose isomerases and their uses
EP2890784A1 (en) 2012-08-29 2015-07-08 Lallemand Hungary Liquidity Management LLC Expression of enzymes in yeast for lignocellulose derived oligomer cbp
US8748152B1 (en) 2012-09-14 2014-06-10 The United States Of America, As Represented By The Secretary Of Agriculture Prevotella ruminicola xylose isomerase and co-expression with xylulokinase in yeast for xylose fermentation
WO2014092832A2 (en) 2012-09-19 2014-06-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
US10035996B2 (en) 2012-10-08 2018-07-31 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
KR20150070151A (ko) 2012-10-16 2015-06-24 디에스엠 아이피 어셋츠 비.브이. 5탄당 전환이 개선된 세포
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
EP2917334A1 (en) 2012-11-07 2015-09-16 DSM IP Assets B.V. pH CONTROLLED YEAST PROPAGATION
AU2013358981A1 (en) 2012-12-14 2015-06-04 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150337280A1 (en) 2012-12-19 2015-11-26 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
US20140178954A1 (en) 2012-12-20 2014-06-26 E I Du Pont De Nemours And Company Expression of xylose isomerase activity in yeast
US11492753B2 (en) 2013-02-15 2022-11-08 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Process for the treatment of lignocellulosic biomass
NL2011164C2 (en) 2013-07-15 2015-01-21 Stichting Energie Improved process for the organosolv treatment of lignocellulosic biomass.
CN105074001A (zh) 2013-02-21 2015-11-18 诺维信公司 糖化和发酵纤维素材料的方法
WO2014138672A1 (en) 2013-03-08 2014-09-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
NZ706072A (en) 2013-03-08 2018-12-21 Xyleco Inc Equipment protecting enclosures
US8669076B1 (en) 2013-03-11 2014-03-11 E I Du Pont De Nemours And Company Cow rumen xylose isomerases active in yeast cells
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
US9340767B2 (en) 2013-03-13 2016-05-17 Poet Research, Inc. Propagating an organism and related methods and compositions
US9034631B2 (en) 2013-03-14 2015-05-19 Poet Research, Inc. Systems and methods for yeast propagation
JP6027559B2 (ja) 2013-03-28 2016-11-16 株式会社豊田中央研究所 キシロースイソメラーゼ活性を有するタンパク質及びその利用
BR112015026063A2 (pt) 2013-04-15 2017-10-17 Katholieke Univ Leuven K U Leuven R&D alelos de levedura envolvidos na capacidade máxima de acúmulo e tolerância a altos níveis de álcool
EP2994529B1 (en) 2013-05-10 2018-11-21 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP3502264A3 (en) 2013-05-31 2019-11-06 DSM IP Assets B.V. Microorganisms for diterpene production
JP5845210B2 (ja) 2013-06-13 2016-01-20 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
CN108064135A (zh) 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 二萜的生产
EP3022300B1 (en) 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN108064226A (zh) 2013-07-31 2018-05-22 帝斯曼知识产权资产管理有限公司 甜菊糖苷的回收
EP3052620B1 (en) 2013-09-04 2020-07-15 Novozymes A/S Processes for increasing enzymatic hydrolysis of cellulosic material
US10053715B2 (en) 2013-10-04 2018-08-21 Corbion Biotech, Inc. Tailored oils
JP6210487B2 (ja) * 2013-10-11 2017-10-11 国立大学法人山口大学 キシロース資化能を有するエタノール生産酵母を生産する方法
CN118325984A (zh) 2013-11-01 2024-07-12 诺维信公司 糖化和发酵纤维素材料的方法
EP3074513A1 (en) 2013-11-26 2016-10-05 Novozymes A/S Enzyme compositions and uses thereof
KR20150065213A (ko) 2013-12-04 2015-06-15 삼성전자주식회사 감소된 에탄올 생산능을 갖는 효모 세포 및 이의 용도
MY186318A (en) 2014-01-07 2021-07-08 Novozymes As Process for degrading mannan-containing cellulosic materials
DK3097192T3 (da) 2014-01-22 2018-11-19 Novozymes As Pullulanasevarianter og polynukleotider, som koder for dem
EP3132045A1 (en) 2014-04-17 2017-02-22 Shell Internationale Research Maatschappij B.V. Processes for producing fermentation products
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
WO2015166645A1 (ja) 2014-04-28 2015-11-05 国立大学法人山口大学 キシロース資化能及びエタノール生産能を有する酵母
MY182924A (en) 2014-06-06 2021-02-05 Novozymes As Enzyme compositions and uses thereof
US10190086B2 (en) 2014-06-24 2019-01-29 Poet Research, Inc. Methods of pitching yeast for fermentation, and related methods of fermentation and systems
CN106574255A (zh) 2014-07-10 2017-04-19 泰拉瑞亚控股公司 酮脂酰acp合酶基因及其用途
US10619173B2 (en) 2014-07-22 2020-04-14 Iogen Corporation Process for using biogenic carbon dioxide derived from non-fossil organic material
WO2016011555A1 (en) 2014-07-22 2016-01-28 Iogen Corporation Process for producing fuel using two fermentations
EP3191597A1 (en) 2014-08-21 2017-07-19 Novozymes A/S Process for saccharifying cellulosic material under oxygen addition
EP3189137A1 (en) 2014-09-05 2017-07-12 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
MX2017003639A (es) 2014-09-23 2017-07-13 Novozymes As Procesos para producir etanol y organismos fermentadores.
CA2961448C (en) 2014-10-23 2023-09-26 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
BR102014027233A2 (pt) * 2014-10-30 2016-05-24 Biocelere Agroindustrial Ltda cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos
JP6303984B2 (ja) 2014-10-31 2018-04-04 トヨタ自動車株式会社 連続培養によるエタノールの製造方法及び連続培養装置
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
US11434509B2 (en) 2014-12-08 2022-09-06 Iogen Corporation Process for using biogenic carbon dioxide derived from non-fossil organic material
US10287610B2 (en) 2015-01-28 2019-05-14 Dsm Ip Assets B.V. Integrated process for coproducing alcohol and organic acid from lignocellulosic material
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3640336B1 (en) 2015-01-28 2022-04-06 DSM IP Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
NL1041192B1 (en) 2015-02-16 2017-01-05 Stichting Energieonderzoek Centrum Nederland Separation of lignin from organosolv liquors.
WO2016138167A2 (en) 2015-02-24 2016-09-01 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US20180044707A1 (en) 2015-03-12 2018-02-15 Novozymes A/S Multi-Stage Enzymatic Hydrolysis of Lignocellulosic Biomass
WO2016145363A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Multi-stage enzymatic hydrolysis of lignocellulosic biomass employing an oxidoreductase with an aa9 polypeptide
BR112017019331A2 (pt) 2015-03-12 2018-06-05 Beta Renewables Spa processos para sacarificação de múltiplos estágios de um material lignocelulósico e para melhorar um rendimento de glicose ou xilose de sacarificação de um material lignocelulósico em um reator de tanque continuamente agitado
US11008598B2 (en) 2015-03-16 2021-05-18 Iogen Corporation Process comprising acid pretreatment and enzymatic hydrolysis
US10995314B2 (en) 2015-03-16 2021-05-04 Iogen Corporation Sulfur dioxide and/or sulfurous acid pretreatment with sulfur dioxide recovery
US10513714B2 (en) 2015-03-16 2019-12-24 Iogen Corporation Lignocellulosic conversion process comprising sulfur dioxide and/or sulfurous acid pretreatment
EP3862426A3 (en) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferases
CN107429238A (zh) 2015-03-23 2017-12-01 帝斯曼知识产权资产管理有限公司 来自番茄的udp‑糖基转移酶
BR112017021066B1 (pt) 2015-04-03 2022-02-08 Dsm Ip Assets B.V. Glicosídeos de esteviol, método para a produção de um glicosídeo de esteviol, composição, usos relacionados, gênero alimentício, alimento para animais e bebida
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
BR112017022298A2 (pt) 2015-04-21 2018-07-10 Dsm Ip Assets Bv geranilgeranil pirofosfato sintase
EP3303578B1 (en) 2015-05-27 2020-07-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2016201184A1 (en) * 2015-06-11 2016-12-15 Ebio, Llc Efficient production of biofuels from cells carrying a metabolic-bypass gene cassette
CN108350442A (zh) 2015-06-18 2018-07-31 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA2991651A1 (en) 2015-07-10 2017-01-19 Dsm Ip Assets B.V. Steviol glycoside composition
EP3322796A4 (en) * 2015-07-13 2019-03-27 Mara Renewables Corporation IMPROVEMENT OF MICROGENONAL FLUID CHANGES OF XYLOSE
US10577594B2 (en) 2015-07-24 2020-03-03 Novozymes, Inc. Polypeptides having arabinofuranosidase activity and polynucleotides encoding same
US20180216089A1 (en) 2015-07-24 2018-08-02 Novozymes, Inc. Polypeptides Having Beta-Xylosidase Activity And Polynucleotides Encoding Same
EP3332004A1 (en) 2015-08-05 2018-06-13 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
WO2017025649A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017050242A1 (en) 2015-09-22 2017-03-30 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US10513715B2 (en) 2015-09-24 2019-12-24 Iogen Corporation Wet oxidation of biomass
WO2017060318A2 (en) 2015-10-05 2017-04-13 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2017070219A1 (en) 2015-10-20 2017-04-27 Novozymes A/S Lytic polysaccharide monooxygenase (lpmo) variants and polynucleotides encoding same
BR112018010741B1 (pt) 2015-11-25 2022-11-22 Iogen Energy Corporation Método para resfriamento e hidrólise de biomassa pré-tratada e sistema para hidrólise de biomassa
US10662455B2 (en) 2015-12-18 2020-05-26 Iogen Corporation Sulfur dioxide and/or sulfurous acid pretreatment
WO2017136915A1 (en) 2016-02-10 2017-08-17 Iogen Corporation Pretreatment of lignocellulosic biomass with sulfur dioxide and/or sulfurous acid
EP3423577B1 (en) 2016-03-02 2024-05-08 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
EP3433358B1 (en) 2016-03-24 2022-07-06 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
BR112018070645A2 (pt) 2016-04-08 2019-02-05 Du Pont células de levedura recombinante, métodos para produzir uma célula de levedura e método para produzir um composto alvo
WO2017205535A1 (en) 2016-05-27 2017-11-30 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
MY197083A (en) 2016-06-09 2023-05-24 Dsm Ip Assets Bv Seed train for large scale enzyme production
JP6447583B2 (ja) 2016-06-24 2019-01-09 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
CN109642225A (zh) 2016-07-21 2019-04-16 诺维信公司 丝氨酸蛋白酶变体以及对其进行编码的多核苷酸
CA3029479A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018026868A1 (en) 2016-08-01 2018-02-08 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
CA3033243A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
BR112019002417B1 (pt) 2016-08-09 2023-05-02 Dsm Ip Assets B.V. Cristalização de glicosídeos de esteviol
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
US11225647B2 (en) 2016-10-27 2022-01-18 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
WO2018085370A1 (en) 2016-11-02 2018-05-11 Novozymes A/S Processes for reducing production of primeverose during enzymatic saccharification of lignocellulosic material
WO2018083301A2 (en) 2016-11-04 2018-05-11 Inbicon A/S Method for preparing fermentable sugars from lignocellulosic biomass
WO2018096019A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme composition
WO2018096017A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme composition
US20210292723A1 (en) 2016-11-29 2021-09-23 Novozymes A/S Alpha-Amylase Variants and Polynucleotides Encoding Same
US20190345522A1 (en) 2016-12-06 2019-11-14 Novozymes A/S Improved Processes For Production Of Ethanol From Xylose-Containing Cellulosic Substrates Using Engineered Yeast Strains
AU2017373570A1 (en) 2016-12-08 2019-06-13 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
BR112019012949A2 (pt) 2016-12-21 2019-11-26 Globalyeast N V isomerases de xilose que conferem capacidade de fermentação eficiente de xilose em levedura
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2018220116A1 (en) 2017-05-31 2018-12-06 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
EP3630989A1 (en) 2017-06-02 2020-04-08 Novozymes A/S Improved yeast for ethanol production
CN110892068B (zh) 2017-06-27 2024-03-08 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
WO2019005755A1 (en) 2017-06-28 2019-01-03 Novozymes A/S POLYPEPTIDES HAVING TREHALASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
BR112020002115A2 (pt) 2017-08-08 2020-08-11 Novozymes A/S polipeptídeo variante de trealase, polinucleotídeo, construto de ácido nucleico, vetor de expressão, célula hospedeira, composição, formulação de caldo inteiro ou composição de cultura de células, método de produção de uma variante de trealase, e, processo de produção de um produto de fermentação
US20200181663A1 (en) 2017-08-30 2020-06-11 Novozymes A/S Combined use of at least one endoprotease and at least one exo-protease in an ssf process for improving ethanol yield
RS65333B1 (sr) 2017-10-09 2024-04-30 Versalis Spa Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA3078156A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA3078822A1 (en) 2017-11-09 2019-05-16 Iogen Corporation Low temperature sulfur dioxide pretreatment
CA3078833A1 (en) 2017-11-09 2019-05-16 Iogen Corporation Low temperature pretreatment with sulfur dioxide
CA3079778A1 (en) 2017-11-14 2019-05-23 Dsm Ip Assets B.V. Polypeptides with improved arabinose transport specificity
US11371012B2 (en) 2017-11-16 2022-06-28 Poet Research, Inc. Methods for propagating microorganisms for fermentation and related methods and systems
CA3085746A1 (en) 2017-12-14 2019-06-20 Poet Research, Inc. Method for propagating microorganisms on a medium comprising stillage
WO2019148192A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
WO2019149789A1 (en) 2018-02-01 2019-08-08 Dsm Ip Assets B.V. Yeast cell capable of simultaneously fermenting hexose and pentose sugars
US20210017526A1 (en) 2018-03-27 2021-01-21 Basf Se Xylose metabolizing yeast
US11193145B2 (en) 2018-03-28 2021-12-07 Dsm Ip Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
WO2019191828A1 (en) 2018-04-06 2019-10-10 Iogen Corporation Pretreatment with lignosulfonic acid
WO2019211230A1 (en) 2018-04-30 2019-11-07 Dsm Ip Assets B.V. Steviol glycoside transport
BR112020023198A2 (pt) 2018-05-17 2021-02-09 Dsm Ip Assets B.V. processo para produção de um polipeptídeo
BR112020024109B1 (pt) 2018-05-30 2023-12-26 Versalis S.P.A Processos para preparação de um produto de açúcar a partir de material de carboidrato
EP3827088A1 (en) 2018-07-25 2021-06-02 Novozymes A/S Enzyme-expressing yeast for ethanol production
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
JP7078900B2 (ja) 2018-10-05 2022-06-01 トヨタ自動車株式会社 形質転換酵母及びこれを用いたエタノールの製造方法
BR112021006692A2 (pt) 2018-10-08 2021-08-10 Novozymes A/S levedura expressando enzimas para produção de etanol
EP3894551A1 (en) 2018-12-12 2021-10-20 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
CN113544280A (zh) 2019-03-12 2021-10-22 帝斯曼知识产权资产管理有限公司 用于产生发酵液的方法
BR112021026761A2 (pt) 2019-07-26 2022-02-15 Novozymes As Célula de levedura, composição, e, métodos de produção de um derivado de uma estirpe de levedura, de produção de etanol e de produção de um produto de fermentação a partir de um material contendo amido ou contendo celulose
CN114127124A (zh) 2019-08-06 2022-03-01 诺维信公司 用于提高酶表达的融合蛋白
BR112022002942A2 (pt) 2019-09-10 2022-05-10 Dsm Ip Assets Bv Composição enzimática
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
CA3191025A1 (en) 2020-09-04 2022-03-10 Roberto Nobuyuki Maeda Improved fermenting organism for ethanol production
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
CA3214435A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
CA3216054A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
CN117178060A (zh) 2021-04-06 2023-12-05 帝斯曼知识产权资产管理有限公司 酶组合物
MX2023010968A (es) 2021-04-08 2023-09-27 Versalis Spa Proceso para la preparacion de un producto de azucar y un producto de fermentacion.
MX2023014545A (es) 2021-06-07 2024-03-01 Novozymes As Microorganismo diseñado para mejorar la fermentación del etanol.

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
US5190877A (en) * 1987-09-03 1993-03-02 Gist-Brocades N.V. Saccharomyces strains for maltose fermentation
ES2198410T3 (es) 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.
GB9502413D0 (en) 1995-02-08 1995-03-29 Primalco Ltd Xylose isomerase
US5935837A (en) 1997-07-28 1999-08-10 Novo Nordisk A/S DNA constructs and methods of producing xylose isomerase
SE9901298D0 (sv) 1999-04-09 1999-04-09 Forskarpatent I Syd Ab Xylose isomerase with improved kinetic properties
EP1468093B2 (en) * 2002-01-23 2018-01-24 DSM IP Assets B.V. Fermentation of pentose sugars
ES2543385T3 (es) 2003-05-02 2015-08-18 Cargill, Incorporated Especies de levadura genéticamente modificadas y procesos de fermentación que emplean levaduras genéticamente modificadas
CN101052706B (zh) 2004-07-16 2015-02-18 帝斯曼知识产权资产管理有限公司 发酵木糖的真核细胞的代谢工程
JP5625199B2 (ja) 2008-03-07 2014-11-19 ディーエスエム アイピー アセッツ ビー．ブイ． ペントース糖発酵細胞
JP5821132B2 (ja) 2008-12-24 2015-11-24 ディーエスエム アイピー アセッツ ビー．ブイ． キシロース異性化酵素遺伝子及びペントース糖の発酵におけるそれらの使用
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
CN103131720A (zh) 2011-11-23 2013-06-05 中国农业科学院作物科学研究所 一种真菌木糖异构酶基因及其应用

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