DK175496B1 - Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin - Google Patents

Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin Download PDF

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Publication number
DK175496B1
DK175496B1 DK198502656A DK265685A DK175496B1 DK 175496 B1 DK175496 B1 DK 175496B1 DK 198502656 A DK198502656 A DK 198502656A DK 265685 A DK265685 A DK 265685A DK 175496 B1 DK175496 B1 DK 175496B1
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DK
Denmark
Prior art keywords
dna
hirudin
desulfatohirudin
solution
transformed
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DK198502656A
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Danish (da)
English (en)
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DK265685D0 (da
DK265685A (da
Inventor
Markus Gerhard Gruetter
Manfred Liersch
Hans Rink
Walter Maerki
Bernd Meyhack
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Ucp Gen Pharma Ag
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Publication of DK265685D0 publication Critical patent/DK265685D0/da
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/38—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK198502656A 1984-06-14 1985-06-13 Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin DK175496B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH288284 1984-06-14
CH288284 1984-06-14

Publications (3)

Publication Number Publication Date
DK265685D0 DK265685D0 (da) 1985-06-13
DK265685A DK265685A (da) 1985-12-15
DK175496B1 true DK175496B1 (da) 2004-11-08

Family

ID=4243708

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198502656A DK175496B1 (da) 1984-06-14 1985-06-13 Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin

Country Status (23)

Country Link
US (2) US5422249A (xx)
EP (1) EP0168342B1 (xx)
JP (1) JPH0720434B2 (xx)
KR (1) KR920009522B1 (xx)
AT (1) ATE64956T1 (xx)
AU (1) AU591061B2 (xx)
CA (1) CA1341426C (xx)
CY (1) CY1782A (xx)
DD (1) DD240392A5 (xx)
DE (1) DE3583361D1 (xx)
DK (1) DK175496B1 (xx)
ES (3) ES8708012A1 (xx)
FI (1) FI90787C (xx)
GR (1) GR851428B (xx)
HK (1) HK11095A (xx)
HU (1) HU202288B (xx)
IE (1) IE57999B1 (xx)
IL (1) IL75508A (xx)
NO (2) NO177433C (xx)
NZ (1) NZ212405A (xx)
PH (1) PH26867A (xx)
PT (1) PT80631B (xx)
ZA (1) ZA854418B (xx)

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US5705355A (en) * 1984-03-27 1998-01-06 Transgene, S.A. Hirudin, pharmaceutical compositions comprising it and their use
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5821079A (en) * 1985-05-02 1998-10-13 Transgene S.A. Vectors for the expression and secretion of hirudin by transformed yeasts
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB2188322A (en) * 1986-03-26 1987-09-30 Bayer Ag Aprotinin and analogues thereof produced by a recombinant host
FR2607516B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
JPH0616716B2 (ja) * 1986-10-14 1994-03-09 塩野義製薬株式会社 酵母におけるヒトｐｓｔｉの製造法
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
US5112615A (en) * 1988-08-03 1992-05-12 New England Deaconess Hospital Corporation Soluble hirudin conjugates
US5167960A (en) * 1988-08-03 1992-12-01 New England Deaconess Hospital Corporation Hirudin-coated biocompatible substance
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
ES2069726T5 (es) * 1989-01-25 1999-09-16 Novartis Ag Anticuerpos monoclonales especificos para hirudina.
JPH037583A (ja) * 1989-02-28 1991-01-14 Canon Inc 部分的二本鎖オリゴヌクレオチド及びオリゴヌクレオチドの形成方法
EP0385410B1 (en) * 1989-02-28 1996-10-02 Canon Kabushiki Kaisha Partially double-stranded oligonucleotide and method for forming oligonucleotide
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
GB9006400D0 (en) 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
US6060451A (en) * 1990-06-15 2000-05-09 The National Research Council Of Canada Thrombin inhibitors based on the amino acid sequence of hirudin
US5296352A (en) * 1990-10-02 1994-03-22 Ciba-Geigy Corporation Monoclonal antibodies directed against complexes formed by thrombin and hirudin
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
DE4209110A1 (de) * 1991-11-26 1993-05-27 Basf Ag Neue thrombininhibitorische proteine aus landblutegeln
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
US5643580A (en) * 1994-10-17 1997-07-01 Surface Genesis, Inc. Biocompatible coating, medical device using the same and methods
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de) * 1995-11-24 1997-05-28 Hoechst Ag Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co. Ltd., Tokio/Tokyo Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
US6623981B2 (en) * 1998-01-27 2003-09-23 Bristol-Myers Squibb Company Detection of patients at risk for developing integrin antagonist/agonist mediated disease states
DE10149678A1 (de) 2001-10-09 2009-03-12 Novel Science International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
US20070016969A1 (en) * 2002-01-18 2007-01-18 Animal Technology Institute Taiwan Expression vector for hirudin and transformed cells and transgenic animals containing said vector
DE10301255A1 (de) 2003-01-15 2004-07-29 Cpi Creative Pharma International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
JP3535151B1 (ja) * 2003-07-10 2004-06-07 株式会社ミナキアドバンス 生酵母菌を成分とする液剤の製造方法及びその液剤
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
KR101915445B1 (ko) * 2007-12-28 2018-11-05 박스알타 인코퍼레이티드 단백질의 역압 여과

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US3432596A (en) * 1967-10-13 1969-03-11 Dresden Arzneimittel Method for producing highly pure hirudine
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US4517294A (en) * 1982-03-03 1985-05-14 Genentech, Inc. Human antithrombin III
GR79124B (xx) * 1982-12-22 1984-10-02 Genentech Inc
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341417C (fr) * 1984-03-27 2003-01-21 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
FR2562088B1 (fr) * 1984-03-27 1987-11-13 Transgene Sa Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3438296A1 (de) * 1984-04-18 1985-11-07 Hoechst Ag, 6230 Frankfurt Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
DE3689525D1 (de) * 1985-07-17 1994-02-24 Hoechst Ag Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel.
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
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FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche

Also Published As

Publication number Publication date
NO177433B (no) 1995-06-06
DE3583361D1 (de) 1991-08-08
HU202288B (en) 1991-02-28
FI852342L (fi) 1985-12-15
ES8707765A1 (es) 1987-08-16
CA1341426C (en) 2003-04-01
EP0168342A1 (de) 1986-01-15
DK265685D0 (da) 1985-06-13
GR851428B (xx) 1985-11-25
DK265685A (da) 1985-12-15
PT80631B (de) 1987-04-28
IL75508A (en) 1991-06-10
IE57999B1 (en) 1993-06-02
ES544126A0 (es) 1987-09-01
KR860000381A (ko) 1986-01-28
FI90787B (fi) 1993-12-15
ES557115A0 (es) 1987-08-16
DD240392A5 (de) 1986-10-29
IE851475L (en) 1985-12-14
PH26867A (en) 1992-11-16
NZ212405A (en) 1989-01-06
PT80631A (de) 1985-07-01
AU4365585A (en) 1985-12-19
KR920009522B1 (ko) 1992-10-17
IL75508A0 (en) 1985-10-31
US5422249A (en) 1995-06-06
EP0168342B1 (de) 1991-07-03
ES8708012A1 (es) 1987-09-01
ES557116A0 (es) 1987-08-16
CY1782A (en) 1995-10-20
NO177433C (no) 1995-09-13
NO1999023I1 (no) 1999-11-17
HK11095A (en) 1995-02-03
NO852389L (no) 1985-12-16
US5728549A (en) 1998-03-17
ATE64956T1 (de) 1991-07-15
FI90787C (fi) 1994-03-25
ZA854418B (en) 1986-02-26
AU591061B2 (en) 1989-11-30
FI852342A0 (fi) 1985-06-12
JPH0720434B2 (ja) 1995-03-08
JPS6119492A (ja) 1986-01-28
ES8707766A1 (es) 1987-08-16
HUT38674A (en) 1986-06-30

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