DK175496B1 - Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin - Google Patents

Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin Download PDF

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Publication number
DK175496B1
DK175496B1 DK198502656A DK265685A DK175496B1 DK 175496 B1 DK175496 B1 DK 175496B1 DK 198502656 A DK198502656 A DK 198502656A DK 265685 A DK265685 A DK 265685A DK 175496 B1 DK175496 B1 DK 175496B1
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DK
Denmark
Prior art keywords
dna
hirudin
desulfatohirudin
solution
transformed
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DK198502656A
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Danish (da)
English (en)
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DK265685D0 (da
DK265685A (da
Inventor
Markus Gerhard Gruetter
Manfred Liersch
Hans Rink
Walter Maerki
Bernd Meyhack
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Ucp Gen Pharma Ag
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Publication of DK265685D0 publication Critical patent/DK265685D0/da
Publication of DK265685A publication Critical patent/DK265685A/da
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/38—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
DK198502656A 1984-06-14 1985-06-13 Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin DK175496B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH288284 1984-06-14
CH288284 1984-06-14

Publications (3)

Publication Number Publication Date
DK265685D0 DK265685D0 (da) 1985-06-13
DK265685A DK265685A (da) 1985-12-15
DK175496B1 true DK175496B1 (da) 2004-11-08

Family

ID=4243708

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198502656A DK175496B1 (da) 1984-06-14 1985-06-13 Ekspressionsvektor, fremgangsmåde til fremstilling deraf, mikroorganisme transformeret dermed, fremgangsmåde til fremstilling af den transformerede mikroorganisme samt fremgangsmåde til fremstilling af biologisk aktive desulfatohirudin

Country Status (23)

Country Link
US (2) US5422249A (fr)
EP (1) EP0168342B1 (fr)
JP (1) JPH0720434B2 (fr)
KR (1) KR920009522B1 (fr)
AT (1) ATE64956T1 (fr)
AU (1) AU591061B2 (fr)
CA (1) CA1341426C (fr)
CY (1) CY1782A (fr)
DD (1) DD240392A5 (fr)
DE (1) DE3583361D1 (fr)
DK (1) DK175496B1 (fr)
ES (3) ES8708012A1 (fr)
FI (1) FI90787C (fr)
GR (1) GR851428B (fr)
HK (1) HK11095A (fr)
HU (1) HU202288B (fr)
IE (1) IE57999B1 (fr)
IL (1) IL75508A (fr)
NO (2) NO177433C (fr)
NZ (1) NZ212405A (fr)
PH (1) PH26867A (fr)
PT (1) PT80631B (fr)
ZA (1) ZA854418B (fr)

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DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
US5821079A (en) * 1985-05-02 1998-10-13 Transgene S.A. Vectors for the expression and secretion of hirudin by transformed yeasts
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
GB2188322A (en) * 1986-03-26 1987-09-30 Bayer Ag Aprotinin and analogues thereof produced by a recombinant host
FR2607516B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
JPH0616716B2 (ja) * 1986-10-14 1994-03-09 塩野義製薬株式会社 酵母におけるヒトｐｓｔｉの製造法
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins
US5112615A (en) * 1988-08-03 1992-05-12 New England Deaconess Hospital Corporation Soluble hirudin conjugates
US5167960A (en) * 1988-08-03 1992-12-01 New England Deaconess Hospital Corporation Hirudin-coated biocompatible substance
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
EP0380443B2 (fr) * 1989-01-25 1999-06-30 Novartis AG Anticorps monoclonaux spécifiques pour hirudin
JPH037583A (ja) * 1989-02-28 1991-01-14 Canon Inc 部分的二本鎖オリゴヌクレオチド及びオリゴヌクレオチドの形成方法
EP0385410B1 (fr) * 1989-02-28 1996-10-02 Canon Kabushiki Kaisha Oligonucléotide à double brin partiel et son procédé de formation
GB9006400D0 (en) 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
US6060451A (en) * 1990-06-15 2000-05-09 The National Research Council Of Canada Thrombin inhibitors based on the amino acid sequence of hirudin
US5296352A (en) * 1990-10-02 1994-03-22 Ciba-Geigy Corporation Monoclonal antibodies directed against complexes formed by thrombin and hirudin
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
DE4209110A1 (de) * 1991-11-26 1993-05-27 Basf Ag Neue thrombininhibitorische proteine aus landblutegeln
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
US5643580A (en) * 1994-10-17 1997-07-01 Surface Genesis, Inc. Biocompatible coating, medical device using the same and methods
DE19529997C1 (de) * 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de) * 1995-11-24 1997-05-28 Hoechst Ag Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
US6623981B2 (en) * 1998-01-27 2003-09-23 Bristol-Myers Squibb Company Detection of patients at risk for developing integrin antagonist/agonist mediated disease states
DE10149678A1 (de) 2001-10-09 2009-03-12 Novel Science International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
US20070016969A1 (en) * 2002-01-18 2007-01-18 Animal Technology Institute Taiwan Expression vector for hirudin and transformed cells and transgenic animals containing said vector
DE10301255A1 (de) 2003-01-15 2004-07-29 Cpi Creative Pharma International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
JP3535151B1 (ja) * 2003-07-10 2004-06-07 株式会社ミナキアドバンス 生酵母菌を成分とする液剤の製造方法及びその液剤
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
NZ586316A (en) * 2007-12-28 2012-06-29 Baxter Int Counter-pressure filtration of proteins

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US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US4517294A (en) * 1982-03-03 1985-05-14 Genentech, Inc. Human antithrombin III
GR79124B (fr) * 1982-12-22 1984-10-02 Genentech Inc
WO1985000831A1 (fr) * 1983-08-10 1985-02-28 Amgen Expression microbienne d'un facteur de croissance semblable a l'insuline
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
EP0158564B1 (fr) * 1984-03-27 1992-07-15 Transgene S.A. Vecteurs d'expression de l'hirudine, cellules transformées et procédé de préparation de l'hirudine
FR2562088B1 (fr) * 1984-03-27 1987-11-13 Transgene Sa Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3438296A1 (de) * 1984-04-18 1985-11-07 Hoechst Ag, 6230 Frankfurt Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
EP0209061B1 (fr) * 1985-07-17 1994-01-12 Hoechst Aktiengesellschaft Peptides à activité anticoagulante, leur procédé de préparation, d'obtention, leur application et les agents les contenant
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche

Also Published As

Publication number Publication date
NZ212405A (en) 1989-01-06
DK265685D0 (da) 1985-06-13
NO852389L (no) 1985-12-16
FI90787B (fi) 1993-12-15
CY1782A (en) 1995-10-20
NO1999023I1 (no) 1999-11-17
IL75508A0 (en) 1985-10-31
EP0168342A1 (fr) 1986-01-15
US5728549A (en) 1998-03-17
FI852342L (fi) 1985-12-15
ES544126A0 (es) 1987-09-01
ES557116A0 (es) 1987-08-16
AU591061B2 (en) 1989-11-30
JPS6119492A (ja) 1986-01-28
NO177433C (no) 1995-09-13
PT80631B (de) 1987-04-28
HU202288B (en) 1991-02-28
ES557115A0 (es) 1987-08-16
KR920009522B1 (ko) 1992-10-17
HUT38674A (en) 1986-06-30
US5422249A (en) 1995-06-06
HK11095A (en) 1995-02-03
IE57999B1 (en) 1993-06-02
FI90787C (fi) 1994-03-25
PT80631A (de) 1985-07-01
DE3583361D1 (de) 1991-08-08
JPH0720434B2 (ja) 1995-03-08
IL75508A (en) 1991-06-10
NO177433B (no) 1995-06-06
FI852342A0 (fi) 1985-06-12
PH26867A (en) 1992-11-16
ES8708012A1 (es) 1987-09-01
GR851428B (fr) 1985-11-25
DD240392A5 (de) 1986-10-29
IE851475L (en) 1985-12-14
DK265685A (da) 1985-12-15
KR860000381A (ko) 1986-01-28
EP0168342B1 (fr) 1991-07-03
ZA854418B (en) 1986-02-26
ES8707766A1 (es) 1987-08-16
ATE64956T1 (de) 1991-07-15
AU4365585A (en) 1985-12-19
ES8707765A1 (es) 1987-08-16
CA1341426C (fr) 2003-04-01

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