DK172695B1 - DNA-afsnit af trp-operonet fra E. coli, plasmider indeholdende DNA-afsnittet, fremgangsmåde til fremstilling af en vektor i - Google Patents

DNA-afsnit af trp-operonet fra E. coli, plasmider indeholdende DNA-afsnittet, fremgangsmåde til fremstilling af en vektor i Download PDF

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Publication number
DK172695B1
DK172695B1 DK198601796A DK179686A DK172695B1 DK 172695 B1 DK172695 B1 DK 172695B1 DK 198601796 A DK198601796 A DK 198601796A DK 179686 A DK179686 A DK 179686A DK 172695 B1 DK172695 B1 DK 172695B1
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Prior art keywords
sequence
dna
coli
vector
plasmid
Prior art date
Application number
DK198601796A
Other languages
Danish (da)
English (en)
Other versions
DK179686A (da
DK179686D0 (da
Inventor
Eugen Uhlmann
Joachim Engels
Friedrich Wengenmayer
Michael Leineweber
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of DK179686D0 publication Critical patent/DK179686D0/da
Publication of DK179686A publication Critical patent/DK179686A/da
Application granted granted Critical
Publication of DK172695B1 publication Critical patent/DK172695B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Electrotherapy Devices (AREA)
  • Complex Calculations (AREA)
  • Advance Control (AREA)
DK198601796A 1985-04-19 1986-04-18 DNA-afsnit af trp-operonet fra E. coli, plasmider indeholdende DNA-afsnittet, fremgangsmåde til fremstilling af en vektor i DK172695B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3514113 1985-04-19
DE19853514113 DE3514113A1 (de) 1985-04-19 1985-04-19 Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression

Publications (3)

Publication Number Publication Date
DK179686D0 DK179686D0 (da) 1986-04-18
DK179686A DK179686A (da) 1986-10-20
DK172695B1 true DK172695B1 (da) 1999-05-31

Family

ID=6268543

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198601796A DK172695B1 (da) 1985-04-19 1986-04-18 DNA-afsnit af trp-operonet fra E. coli, plasmider indeholdende DNA-afsnittet, fremgangsmåde til fremstilling af en vektor i

Country Status (19)

Country Link
EP (1) EP0198415B1 (el)
JP (1) JPH0665317B2 (el)
KR (1) KR940004543B1 (el)
AT (1) ATE44046T1 (el)
AU (1) AU600229B2 (el)
CA (1) CA1321963C (el)
DE (2) DE3514113A1 (el)
DK (1) DK172695B1 (el)
ES (2) ES8704541A1 (el)
FI (1) FI84362C (el)
GR (1) GR861022B (el)
HU (1) HU196458B (el)
IE (1) IE58994B1 (el)
IL (1) IL78529A (el)
NO (1) NO175646C (el)
NZ (1) NZ215858A (el)
PH (1) PH26596A (el)
PT (1) PT82417B (el)
ZA (1) ZA862925B (el)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966849A (en) * 1985-09-20 1990-10-30 President And Fellows Of Harvard College CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression
AU8379991A (en) * 1990-09-14 1992-03-26 Astra Aktiebolag A novel method of generating clones for the expression of unfused proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
DE3430683A1 (de) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt Synthetische regulationsregion

Also Published As

Publication number Publication date
IE861031L (en) 1986-10-19
PH26596A (en) 1992-08-19
DK179686A (da) 1986-10-20
DK179686D0 (da) 1986-04-18
FI861624A (fi) 1986-10-20
EP0198415B1 (de) 1989-06-14
EP0198415A2 (de) 1986-10-22
PT82417A (de) 1986-05-01
IL78529A0 (en) 1986-08-31
HU196458B (en) 1988-11-28
ES554097A0 (es) 1987-04-01
ES8704541A1 (es) 1987-04-01
DE3663956D1 (en) 1989-07-20
NZ215858A (en) 1989-01-06
KR940004543B1 (ko) 1994-05-25
AU5638786A (en) 1986-10-23
AU600229B2 (en) 1990-08-09
GR861022B (en) 1986-08-18
CA1321963C (en) 1993-09-07
FI84362B (fi) 1991-08-15
IL78529A (en) 1991-07-18
NO175646C (no) 1994-11-09
HUT41068A (en) 1987-03-30
ATE44046T1 (de) 1989-06-15
ES8706818A1 (es) 1987-07-01
FI84362C (fi) 1991-11-25
DE3514113A1 (de) 1986-10-23
IE58994B1 (en) 1993-12-15
EP0198415A3 (en) 1986-12-30
JPH0665317B2 (ja) 1994-08-24
KR860008286A (ko) 1986-11-14
JPS61242583A (ja) 1986-10-28
NO175646B (el) 1994-08-01
NO861551L (no) 1986-10-20
ES556233A0 (es) 1987-07-01
FI861624A0 (fi) 1986-04-17
PT82417B (pt) 1988-08-17
ZA862925B (en) 1986-12-30

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Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired