ES8704541A1 - Procedimiento para la preparacion de un vector con el sistema de expresion en trp - Google Patents

Procedimiento para la preparacion de un vector con el sistema de expresion en trp

Info

Publication number
ES8704541A1
ES8704541A1 ES554097A ES554097A ES8704541A1 ES 8704541 A1 ES8704541 A1 ES 8704541A1 ES 554097 A ES554097 A ES 554097A ES 554097 A ES554097 A ES 554097A ES 8704541 A1 ES8704541 A1 ES 8704541A1
Authority
ES
Spain
Prior art keywords
sequence
dna
start codon
ligation
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES554097A
Other languages
English (en)
Other versions
ES554097A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8704541A1 publication Critical patent/ES8704541A1/es
Publication of ES554097A0 publication Critical patent/ES554097A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Electrotherapy Devices (AREA)
  • Complex Calculations (AREA)
  • Advance Control (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN VECTOR CON UN SISTEMA DE EXPRESION EN TRP. COMPRENDE: A) CORTAR CON LA ENZIMA DE RESTRICCION NCO I A UN VECTOR DE E. COLI QUE CONTIENE LA SECUENCIA DE ADN I (5KGTATCGACC) (HELICE CODIFICADORA) SEGUIDA POR 5K ATCG 3K; Y B) LIGAR AL VECTOR CORTADO UNA ESTRUCTURA DE GEN DE LA SECUENCIA ADN II (5K CATGX...3K) (3K Y...5K). SIENDO X E Y UN PAR COMPLEMENTARIO DE NUCLEOTIDOS DETRAS DEL CODON DE INICIACION DEL GEN ESTRUCTURAL. EL GEN ESTRUCTURAL EN EL EXTREMO 5K ES UN GEN PROPIO DEL HOSPEDANTE QUE CODIFICA UNA PROTEINA DE FUSION INSOLUBLE, UN INTERFERON O UNA INTERLEUCINA O SOMATOSTATINA.
ES554097A 1985-04-19 1986-04-17 Procedimiento para la preparacion de un vector con el sistema de expresion en trp Expired ES8704541A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853514113 DE3514113A1 (de) 1985-04-19 1985-04-19 Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression

Publications (2)

Publication Number Publication Date
ES8704541A1 true ES8704541A1 (es) 1987-04-01
ES554097A0 ES554097A0 (es) 1987-04-01

Family

ID=6268543

Family Applications (2)

Application Number Title Priority Date Filing Date
ES554097A Expired ES8704541A1 (es) 1985-04-19 1986-04-17 Procedimiento para la preparacion de un vector con el sistema de expresion en trp
ES556233A Expired ES8706818A1 (es) 1985-04-19 1986-06-19 Procedimiento para preparar un polipeptido

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES556233A Expired ES8706818A1 (es) 1985-04-19 1986-06-19 Procedimiento para preparar un polipeptido

Country Status (19)

Country Link
EP (1) EP0198415B1 (es)
JP (1) JPH0665317B2 (es)
KR (1) KR940004543B1 (es)
AT (1) ATE44046T1 (es)
AU (1) AU600229B2 (es)
CA (1) CA1321963C (es)
DE (2) DE3514113A1 (es)
DK (1) DK172695B1 (es)
ES (2) ES8704541A1 (es)
FI (1) FI84362C (es)
GR (1) GR861022B (es)
HU (1) HU196458B (es)
IE (1) IE58994B1 (es)
IL (1) IL78529A (es)
NO (1) NO175646C (es)
NZ (1) NZ215858A (es)
PH (1) PH26596A (es)
PT (1) PT82417B (es)
ZA (1) ZA862925B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966849A (en) * 1985-09-20 1990-10-30 President And Fellows Of Harvard College CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression
AU8379991A (en) * 1990-09-14 1992-03-26 Astra Aktiebolag A novel method of generating clones for the expression of unfused proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
DE3430683A1 (de) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt Synthetische regulationsregion

Also Published As

Publication number Publication date
EP0198415A2 (de) 1986-10-22
DE3663956D1 (en) 1989-07-20
ES8706818A1 (es) 1987-07-01
NZ215858A (en) 1989-01-06
JPH0665317B2 (ja) 1994-08-24
CA1321963C (en) 1993-09-07
ATE44046T1 (de) 1989-06-15
EP0198415B1 (de) 1989-06-14
AU5638786A (en) 1986-10-23
HU196458B (en) 1988-11-28
DE3514113A1 (de) 1986-10-23
IE58994B1 (en) 1993-12-15
ES556233A0 (es) 1987-07-01
PT82417B (pt) 1988-08-17
KR860008286A (ko) 1986-11-14
EP0198415A3 (en) 1986-12-30
GR861022B (en) 1986-08-18
FI84362B (fi) 1991-08-15
IL78529A (en) 1991-07-18
DK172695B1 (da) 1999-05-31
IL78529A0 (en) 1986-08-31
DK179686A (da) 1986-10-20
FI861624A0 (fi) 1986-04-17
FI84362C (fi) 1991-11-25
DK179686D0 (da) 1986-04-18
ES554097A0 (es) 1987-04-01
JPS61242583A (ja) 1986-10-28
AU600229B2 (en) 1990-08-09
PT82417A (de) 1986-05-01
NO861551L (no) 1986-10-20
NO175646B (es) 1994-08-01
KR940004543B1 (ko) 1994-05-25
IE861031L (en) 1986-10-19
NO175646C (no) 1994-11-09
HUT41068A (en) 1987-03-30
FI861624A (fi) 1986-10-20
ZA862925B (en) 1986-12-30
PH26596A (en) 1992-08-19

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Effective date: 20151230