DK1461426T3 - Eubakterielle RNA-polymerasemutanter til forbedret heterolog genekspression - Google Patents
Eubakterielle RNA-polymerasemutanter til forbedret heterolog genekspressionInfo
- Publication number
- DK1461426T3 DK1461426T3 DK02787465T DK02787465T DK1461426T3 DK 1461426 T3 DK1461426 T3 DK 1461426T3 DK 02787465 T DK02787465 T DK 02787465T DK 02787465 T DK02787465 T DK 02787465T DK 1461426 T3 DK1461426 T3 DK 1461426T3
- Authority
- DK
- Denmark
- Prior art keywords
- product
- interest
- gene expression
- rna polymerase
- production
- Prior art date
Links
- 241001112695 Clostridiales Species 0.000 title abstract 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 title abstract 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 title 1
- 241000186394 Eubacterium Species 0.000 abstract 3
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 238000006467 substitution reaction Methods 0.000 abstract 2
- 108010064250 RNA polymerase beta subunit Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 101150090202 rpoB gene Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200101972 | 2001-12-29 | ||
| DKPA200200274 | 2002-02-21 | ||
| PCT/DK2002/000886 WO2003055996A1 (en) | 2001-12-29 | 2002-12-20 | Eubacterial rna-polymerase mutants with altered product production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1461426T3 true DK1461426T3 (da) | 2008-05-19 |
Family
ID=26069122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK02787465T DK1461426T3 (da) | 2001-12-29 | 2002-12-20 | Eubakterielle RNA-polymerasemutanter til forbedret heterolog genekspression |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US20050095669A1 (https=) |
| EP (1) | EP1461426B1 (https=) |
| JP (3) | JP4741186B2 (https=) |
| CN (2) | CN101851599B (https=) |
| AT (1) | ATE383419T1 (https=) |
| AU (1) | AU2002351750A1 (https=) |
| DE (1) | DE60224579T2 (https=) |
| DK (1) | DK1461426T3 (https=) |
| WO (1) | WO2003055996A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4997420B2 (ja) * | 2006-03-28 | 2012-08-08 | 花王株式会社 | 変異微生物 |
| JP6139139B2 (ja) * | 2012-01-11 | 2017-05-31 | 国立大学法人 岡山大学 | 変異型ＲＮＡポリメラーゼβ−サブユニット遺伝子 |
| KR101594156B1 (ko) * | 2013-06-25 | 2016-02-15 | 씨제이제일제당 주식회사 | Ｌ-라이신 생산능이 향상된 미생물 및 그를 이용하여 ｌ-라이신을 생산하는 방법 |
| JP7025325B2 (ja) * | 2015-10-30 | 2022-02-24 | ダニスコ・ユーエス・インク | タンパク質発現の増強およびその方法 |
| CN106244714B (zh) * | 2016-09-23 | 2019-09-24 | 山西大学 | 一种巨大芽孢杆菌特异性检测的基因芯片 |
| US11407983B2 (en) * | 2018-08-07 | 2022-08-09 | Cj Cheiljedang Corporation | Nucleic acid molecules comprising a variant RpoC coding sequence |
| CN111118091A (zh) * | 2018-10-31 | 2020-05-08 | 宁夏乙征生物工程有限公司 | 一种抗菌肽的生产方法 |
| WO2022094445A1 (en) * | 2020-11-02 | 2022-05-05 | University Of Stuttgart | Genome-modified bacterial strains for large scale bioprocesses |
| CN112608911B (zh) * | 2020-12-18 | 2022-07-05 | 湖州中科工业生物技术中心 | 一种rna聚合酶突变体及其应用 |
| WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
| CN120041420B (zh) * | 2025-04-25 | 2025-10-03 | 天津科技大学 | 一种RNA聚合酶β亚单位突变体及其应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6762040B2 (en) * | 2000-05-24 | 2004-07-13 | Novozymes A/S | Method for increasing gene copy number in a host cell and resulting host cell |
| EP1239040A3 (de) * | 2001-02-16 | 2003-01-08 | Degussa AG | Mutationen im rpoB-Gen L-Lysin produzierender Corynebacterium glutamicum-Stämme und Verfahren zur Herstellung von L-Lysin |
-
2002
- 2002-12-20 JP JP2003556516A patent/JP4741186B2/ja not_active Expired - Fee Related
- 2002-12-20 CN CN201010155784.7A patent/CN101851599B/zh not_active Expired - Lifetime
- 2002-12-20 AU AU2002351750A patent/AU2002351750A1/en not_active Abandoned
- 2002-12-20 DE DE60224579T patent/DE60224579T2/de not_active Expired - Lifetime
- 2002-12-20 US US10/498,302 patent/US20050095669A1/en not_active Abandoned
- 2002-12-20 CN CN02826472XA patent/CN1610740B/zh not_active Expired - Lifetime
- 2002-12-20 AT AT02787465T patent/ATE383419T1/de not_active IP Right Cessation
- 2002-12-20 DK DK02787465T patent/DK1461426T3/da active
- 2002-12-20 WO PCT/DK2002/000886 patent/WO2003055996A1/en not_active Ceased
- 2002-12-20 EP EP02787465A patent/EP1461426B1/en not_active Expired - Lifetime
-
2009
- 2009-08-21 US US12/545,535 patent/US20090317865A1/en not_active Abandoned
-
2011
- 2011-03-03 JP JP2011046487A patent/JP2011155977A/ja not_active Ceased
-
2013
- 2013-01-22 US US13/747,032 patent/US20130122548A1/en not_active Abandoned
- 2013-11-22 JP JP2013241836A patent/JP5876460B2/ja not_active Expired - Fee Related
-
2015
- 2015-02-09 US US14/616,950 patent/US20150147783A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE383419T1 (de) | 2008-01-15 |
| CN101851599A (zh) | 2010-10-06 |
| CN101851599B (zh) | 2016-01-20 |
| CN1610740B (zh) | 2010-05-26 |
| US20150147783A1 (en) | 2015-05-28 |
| DE60224579D1 (de) | 2008-02-21 |
| US20050095669A1 (en) | 2005-05-05 |
| JP4741186B2 (ja) | 2011-08-03 |
| US20090317865A1 (en) | 2009-12-24 |
| CN1610740A (zh) | 2005-04-27 |
| JP2005512595A (ja) | 2005-05-12 |
| US20130122548A1 (en) | 2013-05-16 |
| EP1461426A1 (en) | 2004-09-29 |
| JP2014042530A (ja) | 2014-03-13 |
| DE60224579T2 (de) | 2008-12-24 |
| AU2002351750A1 (en) | 2003-07-15 |
| JP2011155977A (ja) | 2011-08-18 |
| JP5876460B2 (ja) | 2016-03-02 |
| WO2003055996A1 (en) | 2003-07-10 |
| EP1461426B1 (en) | 2008-01-09 |
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