CN101851599B - 具有改变产物产量特性的真细菌rna聚合酶突变体 - Google Patents

具有改变产物产量特性的真细菌rna聚合酶突变体 Download PDF

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Publication number
CN101851599B
CN101851599B CN201010155784.7A CN201010155784A CN101851599B CN 101851599 B CN101851599 B CN 101851599B CN 201010155784 A CN201010155784 A CN 201010155784A CN 101851599 B CN101851599 B CN 101851599B
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polypeptide
enzyme
bacillus
gene
mutant
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Chinese (zh)
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CN101851599A (zh
Inventor
斯蒂恩·T·乔根森
詹斯·T·安德森
尼尔斯·班克
普雷本·尼尔森
金·B·佩德森
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Novo Nordisk AS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1247DNA-directed RNA polymerase (2.7.7.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201010155784.7A 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体 Expired - Lifetime CN101851599B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200101972 2001-12-29
DKPA200101972 2001-12-29
DKPA200200274 2002-02-21
DKPA200200274 2002-02-21
CN02826472XA CN1610740B (zh) 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体

Related Parent Applications (1)

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CN02826472XA Division CN1610740B (zh) 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体

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CN101851599A CN101851599A (zh) 2010-10-06
CN101851599B true CN101851599B (zh) 2016-01-20

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CN201010155784.7A Expired - Lifetime CN101851599B (zh) 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体
CN02826472XA Expired - Lifetime CN1610740B (zh) 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体

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CN02826472XA Expired - Lifetime CN1610740B (zh) 2001-12-29 2002-12-20 具有改变产物产量特性的真细菌rna聚合酶突变体

Country Status (9)

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US (4) US20050095669A1 (https=)
EP (1) EP1461426B1 (https=)
JP (3) JP4741186B2 (https=)
CN (2) CN101851599B (https=)
AT (1) ATE383419T1 (https=)
AU (1) AU2002351750A1 (https=)
DE (1) DE60224579T2 (https=)
DK (1) DK1461426T3 (https=)
WO (1) WO2003055996A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4997420B2 (ja) * 2006-03-28 2012-08-08 花王株式会社 変異微生物
JP6139139B2 (ja) * 2012-01-11 2017-05-31 国立大学法人 岡山大学 変異型RNAポリメラーゼβ−サブユニット遺伝子
KR101594156B1 (ko) 2013-06-25 2016-02-15 씨제이제일제당 주식회사 L-라이신 생산능이 향상된 미생물 및 그를 이용하여 l-라이신을 생산하는 방법
KR20180069057A (ko) 2015-10-30 2018-06-22 다니스코 유에스 인크. 향상된 단백질 발현 및 이의 방법
CN106244714B (zh) * 2016-09-23 2019-09-24 山西大学 一种巨大芽孢杆菌特异性检测的基因芯片
CN112840028B (zh) * 2018-08-07 2024-01-16 Cj第一制糖株式会社 包含变体RpoC编码序列的核酸分子
CN111118091A (zh) * 2018-10-31 2020-05-08 宁夏乙征生物工程有限公司 一种抗菌肽的生产方法
EP4237540A4 (en) * 2020-11-02 2024-11-06 University of Stuttgart BACTERIAL STRAINS WITH MODIFIED GENOME FOR LARGE-SCALE BIOPROCESSES
CN112608911B (zh) * 2020-12-18 2022-07-05 湖州中科工业生物技术中心 一种rna聚合酶突变体及其应用
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
CN120041420B (zh) * 2025-04-25 2025-10-03 天津科技大学 一种RNA聚合酶β亚单位突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1239040A2 (de) * 2001-02-16 2002-09-11 Degussa AG Mutationen im rpoB-Gen L-Lysin produzierender Corynebacterium glutamicum-Stämme und Verfahren zur Herstellung von L-Lysin

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001090393A1 (en) * 2000-05-24 2001-11-29 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1239040A2 (de) * 2001-02-16 2002-09-11 Degussa AG Mutationen im rpoB-Gen L-Lysin produzierender Corynebacterium glutamicum-Stämme und Verfahren zur Herstellung von L-Lysin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ingham C J et al.Mutations in the beta subunit of the Bacillus subtilis RNApolymerase that confer both rifampicin resistance andhypersensitivity to NusG.《Microbiology》.2000,第146卷(第12期),3041-3049. *
Nicholson Wayne L ET AL.The spectrum of spontaneous rifampin resistance mutantionsspores differs from that of Mycobacterium tuberculosis.《Journal of Bacteriology》.2002,第184卷(第17期),4936-4940. *

Also Published As

Publication number Publication date
JP2005512595A (ja) 2005-05-12
US20130122548A1 (en) 2013-05-16
US20050095669A1 (en) 2005-05-05
CN101851599A (zh) 2010-10-06
JP2014042530A (ja) 2014-03-13
CN1610740B (zh) 2010-05-26
DE60224579T2 (de) 2008-12-24
EP1461426A1 (en) 2004-09-29
JP4741186B2 (ja) 2011-08-03
US20090317865A1 (en) 2009-12-24
AU2002351750A1 (en) 2003-07-15
WO2003055996A1 (en) 2003-07-10
EP1461426B1 (en) 2008-01-09
DK1461426T3 (da) 2008-05-19
JP5876460B2 (ja) 2016-03-02
US20150147783A1 (en) 2015-05-28
ATE383419T1 (de) 2008-01-15
CN1610740A (zh) 2005-04-27
DE60224579D1 (de) 2008-02-21
JP2011155977A (ja) 2011-08-18

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