HUP0102310A2 - Vibrio cholerae thyA- törzsek, eljárás előállításukra és alkalmazásuk - Google Patents

Vibrio cholerae thyA- törzsek, eljárás előállításukra és alkalmazásuk

Info

Publication number
HUP0102310A2
HUP0102310A2 HU0102310A HUP0102310A HUP0102310A2 HU P0102310 A2 HUP0102310 A2 HU P0102310A2 HU 0102310 A HU0102310 A HU 0102310A HU P0102310 A HUP0102310 A HU P0102310A HU P0102310 A2 HUP0102310 A2 HU P0102310A2
Authority
HU
Hungary
Prior art keywords
thya
strain
vibrio cholerae
subject
gene
Prior art date
Application number
HU0102310A
Other languages
English (en)
Inventor
Nils Carlin
Michael R. Lebens
Original Assignee
Active Biotech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Active Biotech Ab filed Critical Active Biotech Ab
Publication of HUP0102310A2 publication Critical patent/HUP0102310A2/hu
Publication of HUP0102310A3 publication Critical patent/HUP0102310A3/hu

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C07K14/43559—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from trematodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

A találmány tárgyát képezi eljárás a thyA-Vibrio cholerae törzselőállítására, amely eljárás irányított mutagenezis lépést tartalmaz aV. cholerae kromoszómáján lévő thyA-gén (1. ábra, a szekvencialistában1. azonosítószámon megadott szekvencia) lokuszán. A találmány tárgyatovábbá működésképtelen thyA-gént tartalmazó Vibrio cholerae DthyAtörzs. E törzs tartalmazhat egy vagy több episzómális eredetű, autonómreplikálódó DNS-elemet, pl. plazmidot, amely hordozhat idegen, példáulE. coli eredetű, működésképes thyA-gént, továbbá homológ vagyheterológ proteint kódoló struktúrgént. Továbbá, a találmány tárgyátképezi a thyA-struktúrgén és szegélyező régióinaknukleotidszekvenciái, továbbá általuk kódolt proteinek (4. és 5. ábrántalálható szekvenciák). Ezenfelül, a találmány tárgyát képezi atalálmány szerinti Vibrio cholerae DthyA törzset vagy thyA- törzsettartalmazó vakcina. A találmány szerinti megoldás előnyösenalkalmazható a a protein-előállítás területén, ahol szelekcióra nyújtlehetőséget, továbbá vakcinázáshoz. Ó
HU0102310A 1998-05-26 1999-05-21 Method of producing thy a-strains of vibrio cholerae, such strains and their use HUP0102310A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9801852A SE9801852D0 (sv) 1998-05-26 1998-05-26 Method of producing thy A-strains of Vibro cholerae, suck strains and their use
PCT/EP1999/003509 WO1999061634A1 (en) 1998-05-26 1999-05-21 Method of producing thy a-strains of vibrio cholerae, such strains and their use

Publications (2)

Publication Number Publication Date
HUP0102310A2 true HUP0102310A2 (hu) 2001-10-28
HUP0102310A3 HUP0102310A3 (en) 2003-10-28

Family

ID=20411459

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0102310A HUP0102310A3 (en) 1998-05-26 1999-05-21 Method of producing thy a-strains of vibrio cholerae, such strains and their use

Country Status (18)

Country Link
US (1) US7261899B1 (hu)
EP (1) EP1080211B1 (hu)
AT (1) ATE474055T1 (hu)
AU (1) AU4499999A (hu)
BR (1) BR9910703B1 (hu)
CA (1) CA2329255C (hu)
CZ (1) CZ301662B6 (hu)
DE (1) DE69942579D1 (hu)
DK (1) DK1080211T3 (hu)
EE (1) EE05520B1 (hu)
HK (1) HK1037206A1 (hu)
HU (1) HUP0102310A3 (hu)
IS (1) IS2904B (hu)
NO (1) NO327856B1 (hu)
PL (1) PL197450B1 (hu)
RU (1) RU2265657C2 (hu)
SE (1) SE9801852D0 (hu)
WO (1) WO1999061634A1 (hu)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU22661A1 (es) * 1997-12-30 2001-04-27 Cnic Ct Nac Investigaciones Nuevos candidatos vacunales de vibrio cholerae y método de obtención
SE9801852D0 (sv) * 1998-05-26 1998-05-26 Sbl Vaccin Ab Method of producing thy A-strains of Vibro cholerae, suck strains and their use
GB0121998D0 (en) 2001-09-11 2001-10-31 Acambis Res Ltd Attenuated bacteria useful in vaccines
GB0325494D0 (en) * 2003-10-31 2003-12-03 Sbl Vaccin Ab Expression system
JP2010088301A (ja) * 2007-02-01 2010-04-22 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
US11203761B2 (en) * 2016-08-26 2021-12-21 Codex Dna, Inc. Genetically engineered Vibrio sp. and uses thereof

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470729A (en) * 1983-03-04 1995-11-28 University Of Maryland At Baltimore Method of isolating restriction fragment deletions in Vibrio cholerae, and products thereof
GB2177097A (en) 1985-06-18 1987-01-14 Genencor Inc Stable maintenance of nucleic acid in recombinant cells
EP0251579A3 (en) 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Non-antibiotic marker system
US5198343A (en) 1986-08-05 1993-03-30 Transgene, S.A. Method for expressing a heterologous protein in a dapD- mutant of E. coli and the strain obtained
IE892131A1 (en) * 1989-06-30 1991-01-02 Univ Cork Marker genes for genetic manipulation
EP0692031B1 (en) * 1993-02-22 2007-04-11 The General Hospital Corporation Heterologous antigens in live cell vaccine strains
US5434065A (en) * 1993-05-06 1995-07-18 President And Fellows Of Harvard College In vivo selection of microbial virulence genes
ATE287947T1 (de) * 1994-10-24 2005-02-15 Texas A & M Univ Sys Orale immunisierung durch verwendung von transgenen pflanzen
US6251866B1 (en) * 1997-08-05 2001-06-26 Watson Laboratories, Inc. Conjugates targeted to the interleukin-2 receptor
CU22661A1 (es) * 1997-12-30 2001-04-27 Cnic Ct Nac Investigaciones Nuevos candidatos vacunales de vibrio cholerae y método de obtención
SE9801852D0 (sv) * 1998-05-26 1998-05-26 Sbl Vaccin Ab Method of producing thy A-strains of Vibro cholerae, suck strains and their use

Also Published As

Publication number Publication date
ATE474055T1 (de) 2010-07-15
CA2329255A1 (en) 1999-12-02
CZ20004354A3 (en) 2001-05-16
IS5724A (is) 2000-11-23
EE05520B1 (et) 2012-02-15
NO20005950L (no) 2001-01-25
US7261899B1 (en) 2007-08-28
NO327856B1 (no) 2009-10-05
BR9910703B1 (pt) 2014-10-29
HK1037206A1 (en) 2002-02-01
IS2904B (is) 2014-10-15
RU2265657C2 (ru) 2005-12-10
WO1999061634A1 (en) 1999-12-02
SE9801852D0 (sv) 1998-05-26
NO20005950D0 (no) 2000-11-24
PL197450B1 (pl) 2008-03-31
PL344483A1 (en) 2001-11-05
BR9910703A (pt) 2001-01-30
WO1999061634A9 (en) 2001-12-13
CA2329255C (en) 2009-03-10
HUP0102310A3 (en) 2003-10-28
AU4499999A (en) 1999-12-13
EP1080211B1 (en) 2010-07-14
CZ301662B6 (cs) 2010-05-19
EE200000686A (et) 2002-04-15
DK1080211T3 (da) 2010-10-18
EP1080211A1 (en) 2001-03-07
DE69942579D1 (de) 2010-08-26

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