DK1188841T3 - Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben - Google Patents

Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben

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Publication number
DK1188841T3
DK1188841T3 DK01917537T DK01917537T DK1188841T3 DK 1188841 T3 DK1188841 T3 DK 1188841T3 DK 01917537 T DK01917537 T DK 01917537T DK 01917537 T DK01917537 T DK 01917537T DK 1188841 T3 DK1188841 T3 DK 1188841T3
Authority
DK
Denmark
Prior art keywords
probe
polymer
target gene
complementary
base sequence
Prior art date
Application number
DK01917537T
Other languages
Danish (da)
English (en)
Inventor
Chikako Hakii
Mitsugu Usui
Mari Mitsuka
Original Assignee
Sanko Junyaku Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanko Junyaku Kk filed Critical Sanko Junyaku Kk
Application granted granted Critical
Publication of DK1188841T3 publication Critical patent/DK1188841T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polymerisation Methods In General (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
DK01917537T 2000-03-31 2001-03-28 Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben DK1188841T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000098797 2000-03-31
JP2000309959 2000-10-10
PCT/JP2001/002554 WO2001075157A1 (fr) 2000-03-31 2001-03-28 Sonde servant a fabriquer un polymere sonde, procede de fabrication d'un polymere sonde et son utilisation

Publications (1)

Publication Number Publication Date
DK1188841T3 true DK1188841T3 (da) 2009-04-20

Family

ID=26589206

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01917537T DK1188841T3 (da) 2000-03-31 2001-03-28 Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben

Country Status (13)

Country Link
US (1) US7060814B2 (es)
EP (1) EP1188841B1 (es)
JP (1) JP3310662B2 (es)
KR (1) KR100446358B1 (es)
CN (1) CN1364199B (es)
AT (1) ATE418620T1 (es)
AU (1) AU747848B2 (es)
CA (1) CA2387755C (es)
DE (1) DE60137104D1 (es)
DK (1) DK1188841T3 (es)
ES (1) ES2317893T3 (es)
PT (1) PT1188841E (es)
WO (1) WO2001075157A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175689A1 (en) * 2000-08-30 2003-09-18 Mitsugu Usui Gene detecting method
US20050130139A1 (en) * 2001-09-28 2005-06-16 Mitsugu Usui Method f amplifying dna chip signals
WO2003040367A1 (fr) * 2001-11-08 2003-05-15 Sanko Junyaku Co., Ltd. Procede de formation d'un autoassemblage au moyen d'oligonucleotides synthetises par amplification genique et procede de detection d'un autoassemblage et d'un gene
EP1593746B1 (en) * 2003-02-14 2013-01-09 Eisai R&D Management Co., Ltd. Signal amplification method for detecting expressed gene
CN100532550C (zh) 2003-02-21 2009-08-26 卫材R&D管理有限公司 用于变异基因检测的信号扩增方法
JP2005304489A (ja) * 2004-03-24 2005-11-04 Sysmex Corp 標的物質検出用プローブセット及び標的物質検出方法。
ATE478158T1 (de) * 2004-04-28 2010-09-15 Eisai R&D Man Co Ltd Hybridisierungsverfahren
EP1793004B1 (en) * 2004-09-08 2010-03-31 Eisai R&D Management Co., Ltd. Method of forming signal probe polymer
US7862998B2 (en) 2005-02-28 2011-01-04 Eisai R&D Management Co., Ltd. Assist probe and method of using the same
JP4616881B2 (ja) 2005-02-28 2011-01-19 エーザイ・アール・アンド・ディー・マネジメント株式会社 アシストプローブ及びその利用方法
JP3941828B2 (ja) * 2005-09-15 2007-07-04 トヨタ自動車株式会社 内燃機関の空燃比制御装置
US20090117560A1 (en) * 2005-09-27 2009-05-07 Toshihiko Fujikawa Method of Forming Self Assembly Substance on Microsphere and Method of Detecting Target Analyte
JPWO2007108378A1 (ja) * 2006-03-15 2009-08-06 エーザイ・アール・アンド・ディー・マネジメント株式会社 シグナルプローブポリマーの形成方法
SG185315A1 (en) * 2007-10-24 2012-11-29 Eisai R&D Man Co Ltd Nucleic acid probe, and method of forming probe -polymer
WO2009057702A1 (ja) 2007-10-31 2009-05-07 Eisai R & D Management Co., Ltd. 標的物質検出用ポリマー及び標的物質の検出方法
JP2012070635A (ja) * 2009-01-29 2012-04-12 Eisai R & D Management Co Ltd 核酸の検出方法
US9393309B2 (en) * 2011-04-19 2016-07-19 Kyoto University Self-gelatinizable nucleic acid
CN102703584B (zh) * 2012-04-20 2013-08-21 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法
US20150299788A1 (en) 2012-05-15 2015-10-22 Eisai R&D Management Co., Ltd. Rna detection method and detection kit
US20150105298A1 (en) * 2013-10-10 2015-04-16 The Research Foundation For The State University Of New York Multi-oligomer in situ hybridization probes
TW201936929A (zh) * 2018-02-26 2019-09-16 美商Ｃｙ分子診斷公司 以親和力為導向的富集稀少分子的組成物及方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
CA1260372A (en) 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
AU8103687A (en) 1986-10-14 1988-05-06 Beckman Instruments, Inc. Improved nucleic acid hybridization technique and kit therefor
AU2735988A (en) 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays
US4895325A (en) * 1988-11-10 1990-01-23 Safetran Systems Corporation Railroad gate arm lamp circuit
AU635105B2 (en) * 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
US5216143A (en) * 1991-06-28 1993-06-01 Gen-Probe Products Company Nucleic acid probes to mycobacterium gordonae
US5232831A (en) * 1991-06-28 1993-08-03 Gen-Probe Incorporated Nucleic acid probes to streptococcus pyogenes
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP3947597B2 (ja) * 1997-06-06 2007-07-25 栄研化学株式会社 核酸配列の増幅方法
ATE327344T1 (de) * 1997-07-29 2006-06-15 Polyprobe Inc Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
US6277607B1 (en) * 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
DE60123626T2 (de) * 2000-10-11 2007-01-18 Sanko Junyaku Co., Ltd. Verfahren zur konstruktion von selbstassemlbierenden sonden und verfahren zu ihrer detektion

Also Published As

Publication number Publication date
AU4458301A (en) 2001-10-15
PT1188841E (pt) 2009-03-24
US7060814B2 (en) 2006-06-13
AU747848B2 (en) 2002-05-23
ATE418620T1 (de) 2009-01-15
ES2317893T3 (es) 2009-05-01
EP1188841A4 (en) 2005-01-05
EP1188841B1 (en) 2008-12-24
CA2387755A1 (en) 2001-10-11
KR100446358B1 (ko) 2004-09-01
WO2001075157A1 (fr) 2001-10-11
KR20020033599A (ko) 2002-05-07
CA2387755C (en) 2007-07-03
CN1364199B (zh) 2012-05-09
CN1364199A (zh) 2002-08-14
EP1188841A1 (en) 2002-03-20
US20030008294A1 (en) 2003-01-09
DE60137104D1 (de) 2009-02-05
JP3310662B2 (ja) 2002-08-05

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