DK0550696T3 - Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus - Google Patents

Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Info

Publication number
DK0550696T3
DK0550696T3 DK91920000T DK91920000T DK0550696T3 DK 0550696 T3 DK0550696 T3 DK 0550696T3 DK 91920000 T DK91920000 T DK 91920000T DK 91920000 T DK91920000 T DK 91920000T DK 0550696 T3 DK0550696 T3 DK 0550696T3
Authority
DK
Denmark
Prior art keywords
pct
nucleic acid
enzyme
thermosipho africanus
purified thermostable
Prior art date
Application number
DK91920000T
Other languages
Danish (da)
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Richard D Abramson
I Lawrence Greenfield
Fred L Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0550696T3 publication Critical patent/DK0550696T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Feed For Specific Animals (AREA)
DK91920000T 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus DK0550696T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59049090A 1990-09-28 1990-09-28
PCT/US1991/007076 WO1992006202A1 (fr) 1990-09-28 1991-09-26 Enzyme de polymerase d'acide nucleique purifie thermostable derivee du thermosipho africanus

Publications (1)

Publication Number Publication Date
DK0550696T3 true DK0550696T3 (da) 2001-03-19

Family

ID=24362481

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91920000T DK0550696T3 (da) 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Country Status (10)

Country Link
US (1) US5968799A (fr)
EP (1) EP0550696B1 (fr)
JP (1) JP2582980B2 (fr)
AT (1) ATE197718T1 (fr)
AU (1) AU658378B2 (fr)
CA (1) CA2092317A1 (fr)
DE (1) DE69132477T2 (fr)
DK (1) DK0550696T3 (fr)
ES (1) ES2152923T3 (fr)
WO (1) WO1992006202A1 (fr)

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US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
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US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
JP2007506430A (ja) * 2003-09-23 2007-03-22 ユニヴァーシティー オブ ミズーリ 耐熱性酵素を使用するポリヌクレオチド合成方法
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
WO2008021431A2 (fr) 2006-08-14 2008-02-21 Xdx, Inc. Méthodes et compositions permettant de diagnostiquer et de surveiller l'état de rejet de greffe et de troubles immunitaires
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
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CN103492559B (zh) 2011-04-11 2016-07-06 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
WO2012146260A1 (fr) 2011-04-23 2012-11-01 Biolytix Ag Préparation et utilisation de protéines en biologie moléculaire
WO2012170908A1 (fr) 2011-06-08 2012-12-13 Life Technologies Corporation Conception et développement de nouveaux détergents pour utilisation dans des systèmes pcr
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
ES2668448T3 (es) 2011-12-08 2018-05-18 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
JP6140182B2 (ja) 2011-12-08 2017-05-31 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2015061714A1 (fr) 2013-10-25 2015-04-30 Life Technologies Corporation Nouveaux composés à utiliser dans des systèmes acp et applications correspondantes
CN112760305B (zh) * 2021-01-25 2022-04-29 浙江工业大学 一种栖热腔菌磷酸酶突变体及其应用

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US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
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IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
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ES2134198T3 (es) * 1990-09-28 1999-10-01 Hoffmann La Roche Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.

Also Published As

Publication number Publication date
US5968799A (en) 1999-10-19
ES2152923T3 (es) 2001-02-16
JP2582980B2 (ja) 1997-02-19
DE69132477D1 (en) 2000-12-28
AU658378B2 (en) 1995-04-13
WO1992006202A1 (fr) 1992-04-16
EP0550696A1 (fr) 1993-07-14
DE69132477T2 (de) 2001-06-13
AU8907791A (en) 1992-04-28
CA2092317A1 (fr) 1992-03-29
ATE197718T1 (de) 2000-12-15
JPH06504196A (ja) 1994-05-19
EP0550696B1 (fr) 2000-11-22

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