DE69943292D1 - Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität - Google Patents
Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer StabilitätInfo
- Publication number
- DE69943292D1 DE69943292D1 DE69943292T DE69943292T DE69943292D1 DE 69943292 D1 DE69943292 D1 DE 69943292D1 DE 69943292 T DE69943292 T DE 69943292T DE 69943292 T DE69943292 T DE 69943292T DE 69943292 D1 DE69943292 D1 DE 69943292D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acids
- sequencing
- amplification
- production
- thermodynamic stability
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title 2
- 102000039446 nucleic acids Human genes 0.000 title 2
- 108020004707 nucleic acids Proteins 0.000 title 2
- 230000003321 amplification Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/104,067 US6743605B1 (en) | 1998-06-24 | 1998-06-24 | Linear amplification of specific nucleic acid sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69943292D1 true DE69943292D1 (de) | 2011-05-05 |
Family
ID=22298492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69943292T Expired - Lifetime DE69943292D1 (de) | 1998-06-24 | 1999-06-24 | Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität |
Country Status (6)
| Country | Link |
|---|---|
| US (11) | US6743605B1 (enExample) |
| EP (1) | EP0971039B1 (enExample) |
| JP (3) | JP2000037194A (enExample) |
| CA (1) | CA2273064C (enExample) |
| DE (1) | DE69943292D1 (enExample) |
| IL (1) | IL130477A (enExample) |
Families Citing this family (110)
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| WO2001034838A1 (en) | 1999-11-08 | 2001-05-17 | Eiken Kagaku Kabushiki Kaisha | Method of detecting variation or polymorphism |
| EP2253717B1 (en) * | 2000-04-07 | 2014-04-30 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
| JP2001309785A (ja) * | 2000-05-01 | 2001-11-06 | Eiken Chem Co Ltd | 遺伝子配列間の相違検出方法 |
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| DE60141087D1 (de) | 2000-06-26 | 2010-03-04 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| JP4918210B2 (ja) | 2000-09-19 | 2012-04-18 | 栄研化学株式会社 | ポリヌクレオチドの合成方法 |
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1998
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-
1999
- 1999-06-14 IL IL13047799A patent/IL130477A/en not_active IP Right Cessation
- 1999-06-15 CA CA002273064A patent/CA2273064C/en not_active Expired - Lifetime
- 1999-06-24 JP JP11179056A patent/JP2000037194A/ja not_active Withdrawn
- 1999-06-24 DE DE69943292T patent/DE69943292D1/de not_active Expired - Lifetime
- 1999-06-24 EP EP99112181A patent/EP0971039B1/en not_active Expired - Lifetime
- 1999-11-12 US US09/439,594 patent/US6764821B1/en not_active Expired - Lifetime
-
2002
- 2002-11-22 US US10/303,152 patent/US20050260573A1/en not_active Abandoned
- 2002-11-22 US US10/302,362 patent/US7264930B2/en not_active Expired - Lifetime
- 2002-11-22 US US10/304,035 patent/US7485417B2/en not_active Expired - Fee Related
- 2002-11-27 US US10/305,664 patent/US7713691B2/en not_active Expired - Lifetime
- 2002-11-27 US US10/305,690 patent/US7468245B2/en not_active Expired - Fee Related
- 2002-11-27 US US10/307,108 patent/US8133989B2/en not_active Expired - Fee Related
- 2002-11-29 US US10/306,990 patent/US20030165938A1/en not_active Abandoned
-
2003
- 2003-12-24 JP JP2003428482A patent/JP4493330B2/ja not_active Expired - Lifetime
-
2005
- 2005-04-18 JP JP2005120409A patent/JP4675141B2/ja not_active Expired - Lifetime
-
2007
- 2007-10-26 US US11/925,556 patent/US8288092B2/en not_active Expired - Fee Related
- 2007-10-26 US US11/925,730 patent/US8486633B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2000037194A (ja) | 2000-02-08 |
| US8133989B2 (en) | 2012-03-13 |
| JP2005270108A (ja) | 2005-10-06 |
| US7485417B2 (en) | 2009-02-03 |
| US20030165938A1 (en) | 2003-09-04 |
| US7264930B2 (en) | 2007-09-04 |
| US20030170681A1 (en) | 2003-09-11 |
| JP2004141170A (ja) | 2004-05-20 |
| US20030104460A1 (en) | 2003-06-05 |
| EP0971039B1 (en) | 2011-03-23 |
| IL130477A0 (en) | 2000-06-01 |
| US20030170682A1 (en) | 2003-09-11 |
| US20090286285A1 (en) | 2009-11-19 |
| US7713691B2 (en) | 2010-05-11 |
| US7468245B2 (en) | 2008-12-23 |
| JP4675141B2 (ja) | 2011-04-20 |
| US8288092B2 (en) | 2012-10-16 |
| JP4493330B2 (ja) | 2010-06-30 |
| US6764821B1 (en) | 2004-07-20 |
| US20030165939A1 (en) | 2003-09-04 |
| EP0971039A2 (en) | 2000-01-12 |
| CA2273064A1 (en) | 1999-12-24 |
| US20030165936A1 (en) | 2003-09-04 |
| US8486633B2 (en) | 2013-07-16 |
| CA2273064C (en) | 2008-04-01 |
| EP0971039A3 (en) | 2004-01-28 |
| US20090263789A1 (en) | 2009-10-22 |
| US20050260573A1 (en) | 2005-11-24 |
| US6743605B1 (en) | 2004-06-01 |
| IL130477A (en) | 2004-03-28 |
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