DE69931928T2 - Reversibel inhibierende sonden - Google Patents

Reversibel inhibierende sonden Download PDF

Info

Publication number
DE69931928T2
DE69931928T2 DE69931928T DE69931928T DE69931928T2 DE 69931928 T2 DE69931928 T2 DE 69931928T2 DE 69931928 T DE69931928 T DE 69931928T DE 69931928 T DE69931928 T DE 69931928T DE 69931928 T2 DE69931928 T2 DE 69931928T2
Authority
DE
Germany
Prior art keywords
amplification
probe
sequence
nucleic acid
rna polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69931928T
Other languages
German (de)
English (en)
Other versions
DE69931928D1 (de
Inventor
C. Christopher San Diego ADAMS
T. Steven Santee BRENTANO
P. Gary Foster City SCHROTH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE69931928D1 publication Critical patent/DE69931928D1/de
Application granted granted Critical
Publication of DE69931928T2 publication Critical patent/DE69931928T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69931928T 1998-07-31 1999-07-30 Reversibel inhibierende sonden Expired - Lifetime DE69931928T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9497998P 1998-07-31 1998-07-31
US94979P 1998-07-31
PCT/US1999/017340 WO2000006779A1 (en) 1998-07-31 1999-07-30 Reversible inhibiting probes

Publications (2)

Publication Number Publication Date
DE69931928D1 DE69931928D1 (de) 2006-07-27
DE69931928T2 true DE69931928T2 (de) 2007-01-04

Family

ID=22248313

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69931928T Expired - Lifetime DE69931928T2 (de) 1998-07-31 1999-07-30 Reversibel inhibierende sonden

Country Status (9)

Country Link
US (3) US6297365B1 (https=)
EP (1) EP1100972B1 (https=)
JP (1) JP2002521070A (https=)
AT (1) ATE330031T1 (https=)
AU (1) AU759915B2 (https=)
CA (1) CA2336539A1 (https=)
DE (1) DE69931928T2 (https=)
ES (1) ES2265688T3 (https=)
WO (1) WO2000006779A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2286750T3 (es) 1998-05-01 2007-12-01 Gen-Probe Incorporated Dispositivo para agitar el contenido liquido de un contenedor.
US8337753B2 (en) * 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
ATE330031T1 (de) * 1998-07-31 2006-07-15 Gen Probe Inc Reversibel inhibierende sonden
US7923205B2 (en) * 2000-08-25 2011-04-12 Toshikazu Shiba Method for protecting personal information
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002072889A2 (en) * 2001-01-12 2002-09-19 Applera Corporation Methods and compositions for microarray control
AU2002314734B2 (en) 2001-03-30 2007-08-30 Ge Healthcare Bio-Sciences Ab P450 single nucleotide polymorphism biochip analysis
CA2477670A1 (en) * 2002-03-15 2003-09-25 Arcturus Bioscience, Inc. Improved nucleic acid amplification
EP1954706B1 (en) 2005-11-30 2012-03-28 Epicentre Technologies Corporation Method using reversibly blocked tagging oligonucleotides
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US9458451B2 (en) 2007-06-21 2016-10-04 Gen-Probe Incorporated Multi-channel optical measurement instrument
CA2706444C (en) 2007-11-27 2017-06-06 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
JP5601777B2 (ja) * 2008-02-13 2014-10-08 株式会社ジーンデザイン 新規オリゴヌクレオチド誘導体及びそれから成るＮＦ−κＢデコイ
EP2391452B1 (en) 2009-01-30 2015-06-17 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
WO2011063403A1 (en) * 2009-11-23 2011-05-26 Swift Biosciences, Inc. Devices to extend single stranded target molecules
CA3067868A1 (en) * 2017-06-20 2018-12-27 Ulisse Biomed S.P.A Molecular fingerprinting methods to detect and genotype dna targets through polymerase chain reaction

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4720385A (en) * 1983-03-29 1988-01-19 Miles Laboratories, Inc. Protein compositions substantially free from infectious agents
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5089386A (en) * 1987-09-11 1992-02-18 Gene-Trak Systems Test for listeria
CA2013317A1 (en) 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5811533A (en) * 1990-06-11 1998-09-22 Nexstar Pharmaceuticals, Inc. High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF)
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5567583A (en) 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
ES2168380T3 (es) * 1994-07-15 2002-06-16 Organon Teknika Bv Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico.
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US6020130A (en) * 1995-06-07 2000-02-01 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit DNA polymerases
JP2000514307A (ja) 1996-07-16 2000-10-31 ジェン―プローブ・インコーポレーテッド 増加した標的特異的ｔ▲下ｍ▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法
ATE330031T1 (de) * 1998-07-31 2006-07-15 Gen Probe Inc Reversibel inhibierende sonden
US6279365B1 (en) * 2000-06-12 2001-08-28 Shao-Chien Tseng Cold forging forming method for three-dimensional hollow article

Also Published As

Publication number Publication date
DE69931928D1 (de) 2006-07-27
JP2002521070A (ja) 2002-07-16
ATE330031T1 (de) 2006-07-15
US7122316B2 (en) 2006-10-17
CA2336539A1 (en) 2000-02-10
US20020038016A1 (en) 2002-03-28
AU5246799A (en) 2000-02-21
US6602668B2 (en) 2003-08-05
ES2265688T3 (es) 2007-02-16
EP1100972B1 (en) 2006-06-14
AU759915B2 (en) 2003-05-01
US6297365B1 (en) 2001-10-02
WO2000006779A1 (en) 2000-02-10
EP1100972A1 (en) 2001-05-23
US20030157547A1 (en) 2003-08-21

Similar Documents

Publication Publication Date Title
DE69334092T2 (de) Nachweis von Mycobacterium tuberculosis durch Vervielfältigung von Nukleinsäuresequenzen
DE69619959T2 (de) Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer
DE69931928T2 (de) Reversibel inhibierende sonden
DE69428252T2 (de) Verfahren zur Verbesserung der Nukleinsäureamplifizierung
DE3856455T2 (de) Selektive Amplifikation von Oligonukleotidenzielsequenzen
DE69836012T2 (de) Zwei-schritt hybridisierung und einfang von einem polynukleotid
DE69825240T2 (de) Verfahren zur subtraktiven hybridisierung und differenziellen analyse
DE69030955T2 (de) Nukleinsäureverstärkung
DE68926484T2 (de) RNS-Template mit endverbundener Sonde und Verfahren zur Verwendung
DE69512003T2 (de) Oligonukleotid verwendbar als Primer in einem Amplifikationsverfahren auf der Basis einer Strangverdrängungsreplikation
DE69829328T2 (de) Strangverdrängungsamplifikation von RNA
DE69514773T2 (de) Chimärisches Oligonukleotid und dessen Verwendung zur Herstellung von Nukleinsäure-Transkripten
DE69523360T2 (de) Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung
DE69333891T2 (de) Verfahren zur Nukleinsäurenamplifizierung, Zusammensetzung und Kit
DE69636080T2 (de) Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
DE69736667T2 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE69011101T2 (de) Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.
DE69432586T2 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE69432919T2 (de) Verfahren für den Nachweis spezifischer Polynukleotiden
DE69507646T2 (de) Mikrosatelliteverbindung für detektion genetisches polymorphismen
EP0669395A2 (de) 3'-RNA-Markierung mit Terminaler Transferase
DE69900592T2 (de) Verfahren zur unspezifischen amplifizierung einer nukleinsäure
DE69219627T2 (de) Verfahren zur Bestimmung von einer Nukleinsäuresequenz
DE69125702T2 (de) Enzymatische synthese von oligonukleotiden
EP0545010A1 (de) Verfahren zur spezifischen Vervielfältigung von Nukleinsäuresequenzen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition