ES2168380T3 - Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico. - Google Patents

Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico.

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Publication number
ES2168380T3
ES2168380T3 ES95929679T ES95929679T ES2168380T3 ES 2168380 T3 ES2168380 T3 ES 2168380T3 ES 95929679 T ES95929679 T ES 95929679T ES 95929679 T ES95929679 T ES 95929679T ES 2168380 T3 ES2168380 T3 ES 2168380T3
Authority
ES
Spain
Prior art keywords
template
rna
nucleic acid
dna
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95929679T
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English (en)
Inventor
Roy Sooknanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux BV
Original Assignee
Organon Teknika BV
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Filing date
Publication date
Application filed by Organon Teknika BV filed Critical Organon Teknika BV
Application granted granted Critical
Publication of ES2168380T3 publication Critical patent/ES2168380T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

LA INVENCION SE REFIERE A LA UTILIZACION DE UNA ARN POLIMERASA ADN-DIRIGIDA, PROCARIOTICA O EUCARIOTICA, DE UN TIPO QUE SINTETIZA ARN CELULAR, PARA UN PROCEDIMIENTO DE AMPLIFICACION DE UNA SECUENCIA ESPECIFICA DE UN ACIDO NUCLEICO O DE SU COMPLEMENTO. ASIMISMO, SE REFIERE A UN NUEVO PROCEDIMIENTO DE AMPLIFICACION DE UNA SECUENCIA DE ACIDO NUCLEICO ESPECIFICA. EL PROCEDIMIENTO INCLUYE UNA O MAS REACCIONES QUE PUEDEN TENER LUGAR EN UN UNICO RECIPIENTE DE REACCION. EN UN ASPECTO DEL PROCEDIMIENTO, SE PROPORCIONA, EN UNA PRIMERA REACCION, UNA PRIMERA ARN POLIMERASA QUE UTILIZA UN PRIMER MOLDE DE ADN, PARA SINTETIZAR UN PRIMER MOLDE DE ARN, Y, EN UNA SEGUNDA REACCION, SE PROPORCIONA DICHO PRIMER MOLDE DE ARN Y VARIOS REACTIVOS DIFERENTES, DE FORMA QUE LOS REACTIVOS UTILIZAN EL PRIMER MOLDE DE ARN PARA SINTETIZAR UN SEGUNDO MOLDE DE ADN Y UN SEGUNDO MOLDE DE ARN. A CONTINUACION SE PRODUCE UN CICLO EN EL QUE LOS REACTIVOS UTILIZAN EL SEGUNDO MOLDE DE ARN PARA SINTETIZAR UN TERCER MOLDE DE ADN Y MULTIPLES COPIAS DEL SEGUNDO MOLDE DE ARN. EL SEGUNDO MOLDE DE ARN, ES LA SECUENCIA DE ACIDO NUCLEICO ESPECIFICA, O SU COMPLEMENTO. LA INVENCION INCLUYE UN EQUIPAMIENTO QUE CONTIENE LOS REACTIVOS DE LA INVENCION.
ES95929679T 1994-07-15 1995-07-13 Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico. Expired - Lifetime ES2168380T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27525094A 1994-07-15 1994-07-15

Publications (1)

Publication Number Publication Date
ES2168380T3 true ES2168380T3 (es) 2002-06-16

Family

ID=23051486

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95929679T Expired - Lifetime ES2168380T3 (es) 1994-07-15 1995-07-13 Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico.

Country Status (12)

Country Link
US (1) US6025134A (es)
EP (1) EP0776376B1 (es)
JP (1) JP3881009B2 (es)
KR (1) KR100392551B1 (es)
AT (1) ATE209258T1 (es)
AU (1) AU711115B2 (es)
CA (1) CA2195209C (es)
DE (1) DE69524105T2 (es)
DK (1) DK0776376T3 (es)
ES (1) ES2168380T3 (es)
PT (1) PT776376E (es)
WO (1) WO1996002668A1 (es)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712127A (en) 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
WO1998021361A1 (en) * 1996-11-12 1998-05-22 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
ZA989950B (en) 1997-11-17 1999-05-04 Akzo Nobel Nv Transcription based amplification of double stranded DNA targets
US6338954B1 (en) * 1998-02-27 2002-01-15 Pamgene B.V. Method for the non-specific amplification of nucleic acid
WO2000000638A2 (en) * 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
ATE330031T1 (de) * 1998-07-31 2006-07-15 Gen Probe Inc Reversibel inhibierende sonden
WO2003064679A2 (en) 2002-01-30 2003-08-07 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
EP2108707A3 (en) 2002-05-17 2010-04-14 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Molecular identification of Aspergillus Species
US20040018494A1 (en) * 2002-07-25 2004-01-29 Shurtliff Roxanne N Isothermal transcription based amplification assay for detection and quantification of guanylyl cyclase C
AU2003275127A1 (en) 2002-09-19 2004-04-08 Merial Limited P. ariasi polypeptides, p. perniciosus polypeptides and methods of use
EP2085467B1 (en) 2002-10-29 2013-12-11 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Lutzomyia Longipalpis polypeptides and methods of use
US7863041B2 (en) 2005-10-14 2011-01-04 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Rabies virus vector systems and compositions and methods thereof
CA2646132C (en) 2006-02-13 2019-03-19 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primers and probe for detection and discrimination of influenza virus type a
EP2397855A3 (en) 2006-03-14 2012-03-14 Oregon Health and Science University Methods for detecting a mycobacterium tuberculosis infection
NZ629273A (en) 2006-12-27 2015-02-27 Harvard College Compositions and methods for the treatment of infections and tumors
EP2444410A3 (en) 2007-02-28 2012-08-08 The Govt. Of U.S.A. As Represented By The Secretary Of The Department Of Health And Human Services Brachyury polypeptides and methods for use
JP2011517941A (ja) 2008-04-10 2011-06-23 ジーンニュース・コーポレーション 被験者における結腸直腸癌の確率を決定するための方法および装置
CA2732750A1 (en) 2008-09-22 2010-03-25 The Government Of The United States Of America D.B.A.The Department Of V Eterans Affairs Methods for detecting a mycobacterium tuberculosis infection
CA2744449C (en) 2008-11-28 2019-01-29 Emory University Methods for the treatment of infections and tumors
WO2010096713A2 (en) 2009-02-20 2010-08-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for the diagnosis of age-associated vascular disorders
WO2010138908A1 (en) 2009-05-29 2010-12-02 Ventana Medical Systems, Inc. Igfir gene copy number as a prognostic marker in a non-small cell lung cancer
WO2010138926A1 (en) 2009-05-29 2010-12-02 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Nucleic acids for detection and discrimination of genotypes of chlamydophila psittaci
AU2010253978B2 (en) 2009-05-29 2014-12-11 Ventana Medical Systems, Inc. Methods of scoring gene copy number in a biological sample using in situ hybridization
CN103403026B (zh) 2009-09-25 2016-05-11 美国政府(由卫生和人类服务部的部长所代表) Hiv-1中和抗体及其用途
US9181306B2 (en) 2009-10-16 2015-11-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Insertion of foreign genes in rubella virus and their stable expression in a live, attenuated viral vaccine
WO2011063263A2 (en) 2009-11-20 2011-05-26 Oregon Health & Science University Methods for producing an immune response to tuberculosis
WO2011133433A2 (en) 2010-04-16 2011-10-27 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Real time pcr assay for detection of bacterial respiratory pathogens
US9828637B2 (en) 2010-04-18 2017-11-28 Wake Forest University Health Sciences Methods of predicting predisposition to or risk of kidney disease
EP2582727B8 (en) 2010-06-16 2017-04-19 University of Pittsburgh- Of the Commonwealth System of Higher Education Antibodies to endoplasmin and their use
WO2012006056A2 (en) 2010-06-29 2012-01-12 Oregon Health & Science University Ccr6 as a biomarker of alzheimer's disease
WO2012030711A1 (en) 2010-08-30 2012-03-08 Agrigenetics, Inc. Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics
AU2011296212B2 (en) 2010-08-30 2015-06-11 Dow Agrosciences Llc Activation tagging platform for maize, and resultant tagged population and plants
JP2014509189A (ja) 2011-01-25 2014-04-17 アルマック ダイアグノスティックス リミテッド 結腸ガン遺伝子発現シグネチャーおよび使用方法
US9150644B2 (en) 2011-04-12 2015-10-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human monoclonal antibodies that bind insulin-like growth factor (IGF) I and II
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
WO2013006684A1 (en) 2011-07-05 2013-01-10 The Gov. Of The U.S.A. As Represented By The Secretary Of The Dept. Of Health And Human Services. Hiv-1 genotyping assay for global surveillance of hiv-1 drug resistance
WO2013049535A2 (en) 2011-09-30 2013-04-04 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Influenza vaccine
WO2013071954A1 (en) 2011-11-15 2013-05-23 Université Libre de Bruxelles Streptococcus pneumoniae detection in blood
RU2639517C2 (ru) 2012-02-29 2017-12-21 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Энхансер палочковидного вируса сахарного тростника (scbv) и его применение в функциональной геномике растений
CA2866185C (en) 2012-03-23 2021-04-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pathogenic phlebovirus isolates and compositions and methods of use
WO2013148147A1 (en) 2012-03-26 2013-10-03 The U.S.A., As Represented By The Secretary Dept. Of Health And Human Services Dna methylation analysis for the diagnosis, prognosis and treatment of adrenal neoplasms
US9566329B2 (en) 2012-04-06 2017-02-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Live, attenuated rubella vector to express vaccine antigens
CA2871490C (en) 2012-04-27 2022-10-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of cpg oligonucleotides co-formulated with an antibiotic to accelerate wound healing
WO2013165551A1 (en) 2012-05-03 2013-11-07 The Government Of The Usa As Represented By The Secretary Of The Department Of Health And Human Services Methods of detecting influenza virus
US9394574B2 (en) 2012-06-12 2016-07-19 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for detecting Legionella nucleic acids in a sample
US9631243B2 (en) 2012-06-18 2017-04-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and compositions for identifying JC virus
EP2895191B1 (en) 2012-09-14 2019-06-05 The U.S.A. as represented by the Secretary, Department of Health and Human Services Brachyury protein, adenoviral vectors encoding brachyury protein, and their use
WO2014093702A1 (en) 2012-12-12 2014-06-19 The Usa, As Represented By The Secretary, Department Of Health And Human Services Hiv therapeutics and methods of making and using same
EP2951290B1 (en) 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)
EP2956169B1 (en) 2013-02-12 2018-04-11 THE UNITED STATES OF AMERICA, represented by the S Monoclonal antibodies that neutralize norovirus
WO2014152155A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Massively multiplexed rna sequencing
CN105392882A (zh) 2013-04-19 2016-03-09 加利福尼亚大学董事会 孤星病毒
US10000556B2 (en) 2013-05-09 2018-06-19 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Single-domain VHH antibodies directed to norovirus GI.1 and GII.4 and their use
US10006097B2 (en) 2013-10-22 2018-06-26 The United States of America, as represented by the Secretry, Department of Health and Human Services Compositions and methods for detection and discrimination of influenza viruses
WO2015106003A1 (en) 2014-01-08 2015-07-16 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Ras pathways as markers of protection against hiv and methods to improve vaccine efficacy
EP3107930A1 (en) 2014-02-21 2016-12-28 THE UNITED STATES OF AMERICA, represented by the S Hiv-2 nucleic acids and methods of detection
US20170044631A1 (en) 2014-04-14 2017-02-16 The Unite States of America, as represented by the Secretary, Dept. of Health and Human Services Methods for rapid detection and identification of viral nucleic acids
WO2015199976A1 (en) 2014-06-24 2015-12-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Target activated microdissection
US20170252364A1 (en) 2014-07-30 2017-09-07 Mor Research Applications Ltd. Compositions for treatment of acute lymphoblastic leukemia and methods of use thereof
US10934360B2 (en) 2014-07-31 2021-03-02 The Hong Kong University Of Science And Technology Human monoclonal antibodies against EPHA4 and their use
US11279974B2 (en) 2014-12-01 2022-03-22 The Broad Institute, Inc. Method for in situ determination of nucleic acid proximity
WO2016090170A1 (en) 2014-12-05 2016-06-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services A potent anti-influenza a neuraminidase subtype n1 antibody
WO2016134144A1 (en) 2015-02-20 2016-08-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and reagents for detecting ebola virus
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
AU2016308339A1 (en) 2015-08-18 2018-04-12 Baylor College Of Medicine Methods and compositions for altering function and structure of chromatin loops and/or domains
EP3359651A1 (en) 2015-10-05 2018-08-15 THE UNITED STATES OF AMERICA, represented by the S Human rota virus g9p[6]strain and use as a vaccine
WO2017192589A1 (en) 2016-05-02 2017-11-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to influenza ha and their use and identification
WO2017200790A1 (en) 2016-05-20 2017-11-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Real-time reverse transcriptase-polymerase chain reaction assay with modified probe for the diagnosis of rabies viruses and other lyssaviruses
US11248272B2 (en) 2016-06-27 2022-02-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and compositions for influenza a virus subtyping
JP7086984B2 (ja) 2016-12-06 2022-06-20 オレゴン ステイト ユニバーシティ Ｓｔｒｅｐｔｏｍｙｃｅｓ ｆｕｎｇｉｃｉｄｉｃｕｓの遺伝子組換え株におけるエンデュラシジンの産生を増強するための組成物及び方法
WO2018204175A1 (en) 2017-05-03 2018-11-08 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Rapid detection of zika virus by reverse transcription loop-mediated isothermal amplification
US11466329B2 (en) 2017-06-14 2022-10-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Detection of blaIMP antibacterial resistance genes
GB201710838D0 (en) 2017-07-05 2017-08-16 Ucl Business Plc Bispecific antibodies
KR20200066648A (ko) 2017-09-25 2020-06-10 프레드 헛친슨 켄서 리서치 센터 고효율의 표적화된 원위치 게놈 전체 프로파일링
US20200255828A1 (en) 2017-10-04 2020-08-13 The Broad Institute, Inc. Methods and compositions for altering function and structure of chromatin loops and/or domains
WO2019133727A1 (en) 2017-12-29 2019-07-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Universal influenza virus probe set for enrichment of any influenza virus nucleic acid
US11814674B2 (en) 2018-02-02 2023-11-14 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Random amplification methods for extremely low input nucleic acids
CA3107590A1 (en) 2018-07-27 2020-01-30 Aperta Biosciences, Llc Spinosyn formulations for treatment of demodex-induced ocular and facial conditions
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
WO2022056078A1 (en) 2020-09-11 2022-03-17 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Rnase h-assisted detection assay for rna (radar)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE69034177T2 (de) * 1989-07-11 2005-10-27 Gen-Probe Inc., San Diego Verfahren zur Amplifikation von Nukleinsäuresequenzen
WO1991004340A1 (en) * 1989-09-20 1991-04-04 Cambridge Biotech Corporation In vitro, isothermal nucleic acid amplification
DE69029401T2 (de) * 1989-09-21 1997-07-10 Camborne Ind Plc Wiedergewinnung von schrott

Also Published As

Publication number Publication date
CA2195209C (en) 2008-09-23
EP0776376A1 (en) 1997-06-04
KR100392551B1 (ko) 2003-10-30
AU711115B2 (en) 1999-10-07
DK0776376T3 (da) 2002-02-18
ATE209258T1 (de) 2001-12-15
US6025134A (en) 2000-02-15
EP0776376B1 (en) 2001-11-21
DE69524105D1 (de) 2002-01-03
JP3881009B2 (ja) 2007-02-14
CA2195209A1 (en) 1996-02-01
DE69524105T2 (de) 2002-07-18
WO1996002668A1 (en) 1996-02-01
PT776376E (pt) 2002-05-31
AU3336695A (en) 1996-02-16
JPH11505402A (ja) 1999-05-21

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