WO1998049341A3 - Polynucleotide sequencing using semi-degenerate primers - Google Patents

Polynucleotide sequencing using semi-degenerate primers Download PDF

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Publication number
WO1998049341A3
WO1998049341A3 PCT/GB1998/001233 GB9801233W WO9849341A3 WO 1998049341 A3 WO1998049341 A3 WO 1998049341A3 GB 9801233 W GB9801233 W GB 9801233W WO 9849341 A3 WO9849341 A3 WO 9849341A3
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WO
WIPO (PCT)
Prior art keywords
semi
degenerate primers
oligonucleotide primers
polymerase
polynucleotide
Prior art date
Application number
PCT/GB1998/001233
Other languages
French (fr)
Other versions
WO1998049341A2 (en
Inventor
Andrew Webster
Original Assignee
Andrew Webster
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew Webster filed Critical Andrew Webster
Priority to AU72203/98A priority Critical patent/AU7220398A/en
Priority to EP98919324A priority patent/EP0979307A2/en
Publication of WO1998049341A2 publication Critical patent/WO1998049341A2/en
Publication of WO1998049341A3 publication Critical patent/WO1998049341A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the analysis of polynucleotide molecules comprises the steps of: a) reacting a target polynucleotide with oligonucleotide primers, a polymerase enzyme, and the other reagents necessary for the polymerase reaction, wherein the oligonucleotide primers are chosen such that polymerase products of varying lengths are produced; and b) analysing the products of the said reaction or reactions; wherein the oligonucleotide primers include an array of semi-degenerate primers whose (3') ends comprise variations of one or more nucleotides A, T, G, and C such that the array is complementary to all the polynucleotide sequence.
PCT/GB1998/001233 1997-04-28 1998-04-28 Polynucleotide sequencing using semi-degenerate primers WO1998049341A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU72203/98A AU7220398A (en) 1997-04-28 1998-04-28 Polynucleotide sequencing using semi-degenerate primers
EP98919324A EP0979307A2 (en) 1997-04-28 1998-04-28 Polynucleotide sequencing using semi-degenerate primers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9708606.0A GB9708606D0 (en) 1997-04-28 1997-04-28 Sequencing
GB9708606.0 1997-04-28

Publications (2)

Publication Number Publication Date
WO1998049341A2 WO1998049341A2 (en) 1998-11-05
WO1998049341A3 true WO1998049341A3 (en) 1999-01-28

Family

ID=10811491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/001233 WO1998049341A2 (en) 1997-04-28 1998-04-28 Polynucleotide sequencing using semi-degenerate primers

Country Status (4)

Country Link
EP (1) EP0979307A2 (en)
AU (1) AU7220398A (en)
GB (1) GB9708606D0 (en)
WO (1) WO1998049341A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
AUPQ008799A0 (en) * 1999-04-30 1999-05-27 Tillett, Daniel Genome sequencing
JP2005218301A (en) * 2002-03-06 2005-08-18 Takara Bio Inc Method for base sequencing of nucleic acid

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012014A1 (en) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Molecular tagging system
WO1996041893A1 (en) * 1995-06-09 1996-12-27 The University Of Tennessee Research Corporation Methods for the generation of sequence signatures from nucleic acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012014A1 (en) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Molecular tagging system
WO1996041893A1 (en) * 1995-06-09 1996-12-27 The University Of Tennessee Research Corporation Methods for the generation of sequence signatures from nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANDERSSON S ET AL: "THE MOLECULAR BIOLOGY OF ANDROGENIC 17BETA-HYDROXYSTEROID DEHYDROGENASES", JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 53, no. 1/06, June 1995 (1995-06-01), pages 37 - 39, XP000196679 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS, vol. 13, 1992, pages 718 - 725, XP000199116 *

Also Published As

Publication number Publication date
GB9708606D0 (en) 1997-06-18
AU7220398A (en) 1998-11-24
EP0979307A2 (en) 2000-02-16
WO1998049341A2 (en) 1998-11-05

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