JP2002521070A - 可逆的阻害プローブ - Google Patents
可逆的阻害プローブInfo
- Publication number
- JP2002521070A JP2002521070A JP2000562561A JP2000562561A JP2002521070A JP 2002521070 A JP2002521070 A JP 2002521070A JP 2000562561 A JP2000562561 A JP 2000562561A JP 2000562561 A JP2000562561 A JP 2000562561A JP 2002521070 A JP2002521070 A JP 2002521070A
- Authority
- JP
- Japan
- Prior art keywords
- probe
- region
- nucleic acid
- amplification
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- WAHQBNXSPALNEA-UHFFFAOYSA-L lithium succinate Chemical compound [Li+].[Li+].[O-]C(=O)CCC([O-])=O WAHQBNXSPALNEA-UHFFFAOYSA-L 0.000 description 1
- 229960004254 lithium succinate Drugs 0.000 description 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- JWILWRLEBBNTFH-UOWFLXDJSA-N methyl (2r,3r,4r)-2,3,4,5-tetrahydroxypentanoate Chemical compound COC(=O)[C@H](O)[C@H](O)[C@H](O)CO JWILWRLEBBNTFH-UOWFLXDJSA-N 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 239000002062 molecular scaffold Substances 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 230000018791 negative regulation of catalytic activity Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Natural products 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9497998P | 1998-07-31 | 1998-07-31 | |
| US60/094,979 | 1998-07-31 | ||
| PCT/US1999/017340 WO2000006779A1 (en) | 1998-07-31 | 1999-07-30 | Reversible inhibiting probes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002521070A true JP2002521070A (ja) | 2002-07-16 |
| JP2002521070A5 JP2002521070A5 (https=) | 2007-05-31 |
Family
ID=22248313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000562561A Pending JP2002521070A (ja) | 1998-07-31 | 1999-07-30 | 可逆的阻害プローブ |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6297365B1 (https=) |
| EP (1) | EP1100972B1 (https=) |
| JP (1) | JP2002521070A (https=) |
| AT (1) | ATE330031T1 (https=) |
| AU (1) | AU759915B2 (https=) |
| CA (1) | CA2336539A1 (https=) |
| DE (1) | DE69931928T2 (https=) |
| ES (1) | ES2265688T3 (https=) |
| WO (1) | WO2000006779A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010090103A (ja) * | 2008-02-13 | 2010-04-22 | Gene Design Inc | 新規オリゴヌクレオチド誘導体及びそれから成るＮＦ−κＢデコイ |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2286750T3 (es) | 1998-05-01 | 2007-12-01 | Gen-Probe Incorporated | Dispositivo para agitar el contenido liquido de un contenedor. |
| US8337753B2 (en) * | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
| ATE330031T1 (de) * | 1998-07-31 | 2006-07-15 | Gen Probe Inc | Reversibel inhibierende sonden |
| US7923205B2 (en) * | 2000-08-25 | 2011-04-12 | Toshikazu Shiba | Method for protecting personal information |
| US6794141B2 (en) | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
| WO2002072889A2 (en) * | 2001-01-12 | 2002-09-19 | Applera Corporation | Methods and compositions for microarray control |
| AU2002314734B2 (en) | 2001-03-30 | 2007-08-30 | Ge Healthcare Bio-Sciences Ab | P450 single nucleotide polymorphism biochip analysis |
| CA2477670A1 (en) * | 2002-03-15 | 2003-09-25 | Arcturus Bioscience, Inc. | Improved nucleic acid amplification |
| EP1954706B1 (en) | 2005-11-30 | 2012-03-28 | Epicentre Technologies Corporation | Method using reversibly blocked tagging oligonucleotides |
| US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| US9458451B2 (en) | 2007-06-21 | 2016-10-04 | Gen-Probe Incorporated | Multi-channel optical measurement instrument |
| CA2706444C (en) | 2007-11-27 | 2017-06-06 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step rt-pcr |
| EP2391452B1 (en) | 2009-01-30 | 2015-06-17 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
| WO2011063403A1 (en) * | 2009-11-23 | 2011-05-26 | Swift Biosciences, Inc. | Devices to extend single stranded target molecules |
| CA3067868A1 (en) * | 2017-06-20 | 2018-12-27 | Ulisse Biomed S.P.A | Molecular fingerprinting methods to detect and genotype dna targets through polymerase chain reaction |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996041010A1 (en) * | 1995-06-07 | 1996-12-19 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit dna polymerases |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4720385A (en) * | 1983-03-29 | 1988-01-19 | Miles Laboratories, Inc. | Protein compositions substantially free from infectious agents |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5089386A (en) * | 1987-09-11 | 1992-02-18 | Gene-Trak Systems | Test for listeria |
| CA2013317A1 (en) | 1989-04-17 | 1990-10-17 | Fred T. Oakes | Diagnostic kit, primer composition and their use for replication or detection of nucleic acids |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| WO1991001384A1 (en) | 1989-07-11 | 1991-02-07 | Gen-Probe, Incorporated | Nucleic acid sequence amplification methods utilizing a transcription complex |
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| US5215899A (en) * | 1989-11-09 | 1993-06-01 | Miles Inc. | Nucleic acid amplification employing ligatable hairpin probe and transcription |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
| US5811533A (en) * | 1990-06-11 | 1998-09-22 | Nexstar Pharmaceuticals, Inc. | High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF) |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US5567583A (en) | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| KR100249110B1 (ko) * | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5861273A (en) * | 1993-12-21 | 1999-01-19 | Celtrix Phamraceuticals, Inc. | Chromosomal expression of heterologous genes in bacterial cells |
| ES2168380T3 (es) * | 1994-07-15 | 2002-06-16 | Organon Teknika Bv | Utilizacion de arn polimerasa para mejorar un procedimiento de amplificacion de acido nucleico. |
| GB2293238A (en) | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| JP2000514307A (ja) | 1996-07-16 | 2000-10-31 | ジェン―プローブ・インコーポレーテッド | 増加した標的特異的ｔ▲下ｍ▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法 |
| ATE330031T1 (de) * | 1998-07-31 | 2006-07-15 | Gen Probe Inc | Reversibel inhibierende sonden |
| US6279365B1 (en) * | 2000-06-12 | 2001-08-28 | Shao-Chien Tseng | Cold forging forming method for three-dimensional hollow article |
-
1999
- 1999-07-30 AT AT99937681T patent/ATE330031T1/de not_active IP Right Cessation
- 1999-07-30 AU AU52467/99A patent/AU759915B2/en not_active Ceased
- 1999-07-30 US US09/365,121 patent/US6297365B1/en not_active Expired - Lifetime
- 1999-07-30 DE DE69931928T patent/DE69931928T2/de not_active Expired - Lifetime
- 1999-07-30 CA CA002336539A patent/CA2336539A1/en not_active Abandoned
- 1999-07-30 EP EP99937681A patent/EP1100972B1/en not_active Expired - Lifetime
- 1999-07-30 JP JP2000562561A patent/JP2002521070A/ja active Pending
- 1999-07-30 ES ES99937681T patent/ES2265688T3/es not_active Expired - Lifetime
- 1999-07-30 WO PCT/US1999/017340 patent/WO2000006779A1/en not_active Ceased
-
2001
- 2001-05-29 US US09/867,193 patent/US6602668B2/en not_active Expired - Fee Related
-
2003
- 2003-02-27 US US10/375,623 patent/US7122316B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996041010A1 (en) * | 1995-06-07 | 1996-12-19 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit dna polymerases |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010090103A (ja) * | 2008-02-13 | 2010-04-22 | Gene Design Inc | 新規オリゴヌクレオチド誘導体及びそれから成るＮＦ−κＢデコイ |
Also Published As
| Publication number | Publication date |
|---|---|
| DE69931928D1 (de) | 2006-07-27 |
| ATE330031T1 (de) | 2006-07-15 |
| US7122316B2 (en) | 2006-10-17 |
| CA2336539A1 (en) | 2000-02-10 |
| US20020038016A1 (en) | 2002-03-28 |
| AU5246799A (en) | 2000-02-21 |
| US6602668B2 (en) | 2003-08-05 |
| DE69931928T2 (de) | 2007-01-04 |
| ES2265688T3 (es) | 2007-02-16 |
| EP1100972B1 (en) | 2006-06-14 |
| AU759915B2 (en) | 2003-05-01 |
| US6297365B1 (en) | 2001-10-02 |
| WO2000006779A1 (en) | 2000-02-10 |
| EP1100972A1 (en) | 2001-05-23 |
| US20030157547A1 (en) | 2003-08-21 |
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