JP2002521070A - 可逆的阻害プローブ - Google Patents
可逆的阻害プローブInfo
- Publication number
- JP2002521070A JP2002521070A JP2000562561A JP2000562561A JP2002521070A JP 2002521070 A JP2002521070 A JP 2002521070A JP 2000562561 A JP2000562561 A JP 2000562561A JP 2000562561 A JP2000562561 A JP 2000562561A JP 2002521070 A JP2002521070 A JP 2002521070A
- Authority
- JP
- Japan
- Prior art keywords
- probe
- region
- nucleic acid
- amplification
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- WAHQBNXSPALNEA-UHFFFAOYSA-L lithium succinate Chemical compound [Li+].[Li+].[O-]C(=O)CCC([O-])=O WAHQBNXSPALNEA-UHFFFAOYSA-L 0.000 description 1
- 229960004254 lithium succinate Drugs 0.000 description 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- JWILWRLEBBNTFH-UOWFLXDJSA-N methyl (2r,3r,4r)-2,3,4,5-tetrahydroxypentanoate Chemical compound COC(=O)[C@H](O)[C@H](O)[C@H](O)CO JWILWRLEBBNTFH-UOWFLXDJSA-N 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 239000002062 molecular scaffold Substances 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 230000018791 negative regulation of catalytic activity Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Natural products 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9497998P | 1998-07-31 | 1998-07-31 | |
| US60/094,979 | 1998-07-31 | ||
| PCT/US1999/017340 WO2000006779A1 (en) | 1998-07-31 | 1999-07-30 | Reversible inhibiting probes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002521070A true JP2002521070A (ja) | 2002-07-16 |
| JP2002521070A5 JP2002521070A5 (https=) | 2007-05-31 |
Family
ID=22248313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000562561A Pending JP2002521070A (ja) | 1998-07-31 | 1999-07-30 | 可逆的阻害プローブ |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6297365B1 (https=) |
| EP (1) | EP1100972B1 (https=) |
| JP (1) | JP2002521070A (https=) |
| AT (1) | ATE330031T1 (https=) |
| AU (1) | AU759915B2 (https=) |
| CA (1) | CA2336539A1 (https=) |
| DE (1) | DE69931928T2 (https=) |
| ES (1) | ES2265688T3 (https=) |
| WO (1) | WO2000006779A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010090103A (ja) * | 2008-02-13 | 2010-04-22 | Gene Design Inc | 新規オリゴヌクレオチド誘導体及びそれから成るＮＦ−κＢデコイ |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10011529T1 (de) | 1998-05-01 | 2011-09-01 | Gen-Probe Incorporated | Automatisches Diagnoseanalysegerät und Verfahren |
| US8337753B2 (en) * | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
| ATE330031T1 (de) * | 1998-07-31 | 2006-07-15 | Gen Probe Inc | Reversibel inhibierende sonden |
| AU2001280153A1 (en) * | 2000-08-25 | 2002-03-04 | Fujirebio Inc. | Method of protecting personal information |
| US6794141B2 (en) * | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
| WO2002072889A2 (en) * | 2001-01-12 | 2002-09-19 | Applera Corporation | Methods and compositions for microarray control |
| US6986992B2 (en) | 2001-03-30 | 2006-01-17 | Amersham Biosciences Ab | P450 single nucleotide polymorphism biochip analysis |
| EP1485503A4 (en) * | 2002-03-15 | 2005-12-28 | Arcturus Bioscience Inc | IMPROVED NUCLEIC ACID AMPLIFICATION |
| ATE551349T1 (de) * | 2005-11-30 | 2012-04-15 | Epict Technologies Corp | Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden |
| US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| CA2754884C (en) | 2007-06-21 | 2013-04-30 | Gen-Probe Incorporated | Methods of concentrating an analyte |
| CA2964559C (en) | 2007-11-27 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step rt-pcr |
| US8368882B2 (en) | 2009-01-30 | 2013-02-05 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
| EP2504450B1 (en) * | 2009-11-23 | 2016-07-06 | Swift Biosciences, Inc. | Devices to extend single stranded target molecules |
| US20200123598A1 (en) * | 2017-06-20 | 2020-04-23 | Ulisse Biomed S.P.A. | Molecular fingerprinting methods to detect and genotype dna targets through polymerase chain reaction |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996041010A1 (en) * | 1995-06-07 | 1996-12-19 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit dna polymerases |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4720385A (en) * | 1983-03-29 | 1988-01-19 | Miles Laboratories, Inc. | Protein compositions substantially free from infectious agents |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5089386A (en) * | 1987-09-11 | 1992-02-18 | Gene-Trak Systems | Test for listeria |
| CA2013317A1 (en) | 1989-04-17 | 1990-10-17 | Fred T. Oakes | Diagnostic kit, primer composition and their use for replication or detection of nucleic acids |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| FI911152A7 (fi) | 1989-07-11 | 1991-03-07 | Gen Probe Inc | Nukleiinihapposekvenssin amplifikaatiomenetelmät |
| US5215899A (en) * | 1989-11-09 | 1993-06-01 | Miles Inc. | Nucleic acid amplification employing ligatable hairpin probe and transcription |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
| US5811533A (en) * | 1990-06-11 | 1998-09-22 | Nexstar Pharmaceuticals, Inc. | High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF) |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US5567583A (en) | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| JP4137996B2 (ja) * | 1992-05-06 | 2008-08-20 | ジェン−プローブ・インコーポレイテッド | 核酸配列増幅方法，組成物及びキット |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5861273A (en) * | 1993-12-21 | 1999-01-19 | Celtrix Phamraceuticals, Inc. | Chromosomal expression of heterologous genes in bacterial cells |
| DE69524105T2 (de) * | 1994-07-15 | 2002-07-18 | Organon Teknika B.V., Boxtel | Verwendung von rna-polymerase zur verbesserung von nukeinsaeure-amplifikationsverfahren |
| GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| WO1998002582A2 (en) | 1996-07-16 | 1998-01-22 | Gen-Probe Incorporated | Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific t¿m? |
| ATE330031T1 (de) * | 1998-07-31 | 2006-07-15 | Gen Probe Inc | Reversibel inhibierende sonden |
| US6279365B1 (en) * | 2000-06-12 | 2001-08-28 | Shao-Chien Tseng | Cold forging forming method for three-dimensional hollow article |
-
1999
- 1999-07-30 AT AT99937681T patent/ATE330031T1/de not_active IP Right Cessation
- 1999-07-30 ES ES99937681T patent/ES2265688T3/es not_active Expired - Lifetime
- 1999-07-30 CA CA002336539A patent/CA2336539A1/en not_active Abandoned
- 1999-07-30 AU AU52467/99A patent/AU759915B2/en not_active Ceased
- 1999-07-30 US US09/365,121 patent/US6297365B1/en not_active Expired - Lifetime
- 1999-07-30 JP JP2000562561A patent/JP2002521070A/ja active Pending
- 1999-07-30 DE DE69931928T patent/DE69931928T2/de not_active Expired - Lifetime
- 1999-07-30 EP EP99937681A patent/EP1100972B1/en not_active Expired - Lifetime
- 1999-07-30 WO PCT/US1999/017340 patent/WO2000006779A1/en not_active Ceased
-
2001
- 2001-05-29 US US09/867,193 patent/US6602668B2/en not_active Expired - Fee Related
-
2003
- 2003-02-27 US US10/375,623 patent/US7122316B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996041010A1 (en) * | 1995-06-07 | 1996-12-19 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit dna polymerases |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010090103A (ja) * | 2008-02-13 | 2010-04-22 | Gene Design Inc | 新規オリゴヌクレオチド誘導体及びそれから成るＮＦ−κＢデコイ |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE330031T1 (de) | 2006-07-15 |
| DE69931928T2 (de) | 2007-01-04 |
| CA2336539A1 (en) | 2000-02-10 |
| EP1100972B1 (en) | 2006-06-14 |
| WO2000006779A1 (en) | 2000-02-10 |
| US20030157547A1 (en) | 2003-08-21 |
| DE69931928D1 (de) | 2006-07-27 |
| US20020038016A1 (en) | 2002-03-28 |
| EP1100972A1 (en) | 2001-05-23 |
| US7122316B2 (en) | 2006-10-17 |
| ES2265688T3 (es) | 2007-02-16 |
| AU5246799A (en) | 2000-02-21 |
| US6602668B2 (en) | 2003-08-05 |
| AU759915B2 (en) | 2003-05-01 |
| US6297365B1 (en) | 2001-10-02 |
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