DE69930765D1 - Methode zur kontrollierten verlängerung eines oligonukleotids - Google Patents

Methode zur kontrollierten verlängerung eines oligonukleotids

Info

Publication number
DE69930765D1
DE69930765D1 DE69930765T DE69930765T DE69930765D1 DE 69930765 D1 DE69930765 D1 DE 69930765D1 DE 69930765 T DE69930765 T DE 69930765T DE 69930765 T DE69930765 T DE 69930765T DE 69930765 D1 DE69930765 D1 DE 69930765D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
polynucleotide sequence
target polynucleotide
binding substance
extension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69930765T
Other languages
English (en)
Other versions
DE69930765T2 (de
Inventor
Nurith Kurn
Ping Liu
Alla Lishanski
Marc Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aventis Pharmaceuticals Inc
Original Assignee
Aventis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharmaceuticals Inc filed Critical Aventis Pharmaceuticals Inc
Application granted granted Critical
Publication of DE69930765D1 publication Critical patent/DE69930765D1/de
Publication of DE69930765T2 publication Critical patent/DE69930765T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69930765T 1999-01-19 1999-12-10 Methode zur kontrollierten verlängerung eines oligonukleotids Expired - Lifetime DE69930765T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/233,413 US6200757B1 (en) 1999-01-19 1999-01-19 Method for controlling the extension of an oligonucleotide
US233413 1999-01-19
PCT/US1999/029253 WO2000043544A1 (en) 1999-01-19 1999-12-10 Method for controlling the extension of an oligonucleotide

Publications (2)

Publication Number Publication Date
DE69930765D1 true DE69930765D1 (de) 2006-05-18
DE69930765T2 DE69930765T2 (de) 2006-11-23

Family

ID=22877144

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69930765T Expired - Lifetime DE69930765T2 (de) 1999-01-19 1999-12-10 Methode zur kontrollierten verlängerung eines oligonukleotids

Country Status (8)

Country Link
US (1) US6200757B1 (de)
EP (1) EP1147230B1 (de)
JP (1) JP4657454B2 (de)
AT (1) ATE322557T1 (de)
CA (1) CA2358992C (de)
DE (1) DE69930765T2 (de)
HK (1) HK1042324B (de)
WO (1) WO2000043544A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
WO2001062975A2 (en) * 2000-02-23 2001-08-30 City Of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
CA2521127C (en) * 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP2489746B1 (de) 2005-06-07 2016-02-03 Luminex Corporation Verfahren zur Detektion und Typisierung von Nucleinsäuren
US20070048758A1 (en) * 2005-06-09 2007-03-01 Epoch Biosciences, Inc. Improved primer-based amplification methods
AU2006287548A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
ES2357746T3 (es) * 2006-03-28 2011-04-29 Commonwealth Scientific And Industrial Research Organisation Amplificación de fragmentos de adn.
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
WO2010046067A1 (en) * 2008-10-20 2010-04-29 Roche Diagnostics Gmbh Improved allele-specific amplification
AU2010307991A1 (en) * 2009-10-13 2012-05-10 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
EP3502272A1 (de) 2010-06-21 2019-06-26 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
US9005895B2 (en) 2010-06-21 2015-04-14 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
CN108018343B (zh) 2012-05-10 2023-01-03 通用医疗公司 用于测定核苷酸序列的方法
CN106661612A (zh) 2014-01-27 2017-05-10 通用医疗公司 制备用于测序的核酸的方法
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
ES2903357T3 (es) 2016-09-15 2022-04-01 Archerdx Llc Métodos de preparación de muestras de ácido nucleico
AU2019276719A1 (en) * 2018-05-31 2020-11-26 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
KR950013953B1 (ko) 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
ATE188509T1 (de) 1990-02-16 2000-01-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
FR2722799B1 (fr) 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
WO1997023646A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
CA2217143A1 (en) * 1996-02-01 1997-08-07 Behringwerke Aktiengesellschaft Positive controls for use in polynucleotide amplification
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
DK0866071T3 (da) * 1997-03-20 2005-01-17 Hoffmann La Roche Modificerede primere

Also Published As

Publication number Publication date
DE69930765T2 (de) 2006-11-23
HK1042324B (zh) 2006-06-23
US6200757B1 (en) 2001-03-13
HK1042324A1 (en) 2002-08-09
EP1147230A1 (de) 2001-10-24
CA2358992A1 (en) 2000-07-27
CA2358992C (en) 2010-10-12
WO2000043544A1 (en) 2000-07-27
JP4657454B2 (ja) 2011-03-23
JP2002534986A (ja) 2002-10-22
EP1147230B1 (de) 2006-04-05
ATE322557T1 (de) 2006-04-15

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN

8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC