DE69900813T2 - Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher - Google Patents

Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher

Info

Publication number
DE69900813T2
DE69900813T2 DE69900813T DE69900813T DE69900813T2 DE 69900813 T2 DE69900813 T2 DE 69900813T2 DE 69900813 T DE69900813 T DE 69900813T DE 69900813 T DE69900813 T DE 69900813T DE 69900813 T2 DE69900813 T2 DE 69900813T2
Authority
DE
Germany
Prior art keywords
dye
reporter
quencher
oligonucleotide
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69900813T
Other languages
German (de)
English (en)
Other versions
DE69900813D1 (de
Inventor
J. Graham
T. Haxo
G. Lee
B. Mullah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PE Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PE Corp filed Critical PE Corp
Application granted granted Critical
Publication of DE69900813D1 publication Critical patent/DE69900813D1/de
Publication of DE69900813T2 publication Critical patent/DE69900813T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69900813T 1998-01-23 1999-01-19 Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher Expired - Lifetime DE69900813T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/012,525 US6080868A (en) 1998-01-23 1998-01-23 Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
PCT/US1999/001163 WO1999037717A1 (en) 1998-01-23 1999-01-19 Asymmetric cyanine dye quenchers

Publications (2)

Publication Number Publication Date
DE69900813D1 DE69900813D1 (de) 2002-03-14
DE69900813T2 true DE69900813T2 (de) 2002-10-02

Family

ID=21755376

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69900813T Expired - Lifetime DE69900813T2 (de) 1998-01-23 1999-01-19 Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher

Country Status (8)

Country Link
US (5) US6080868A (enExample)
EP (1) EP1049744B1 (enExample)
JP (2) JP3946443B2 (enExample)
AT (1) ATE212367T1 (enExample)
AU (1) AU740989B2 (enExample)
CA (1) CA2318160C (enExample)
DE (1) DE69900813T2 (enExample)
WO (1) WO1999037717A1 (enExample)

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Also Published As

Publication number Publication date
ATE212367T1 (de) 2002-02-15
JP2002501102A (ja) 2002-01-15
JP3946443B2 (ja) 2007-07-18
US6750024B2 (en) 2004-06-15
CA2318160A1 (en) 1999-07-29
CA2318160C (en) 2005-11-29
AU740989B2 (en) 2001-11-22
WO1999037717A1 (en) 1999-07-29
US7166715B2 (en) 2007-01-23
US20050009058A1 (en) 2005-01-13
JP4471711B2 (ja) 2010-06-02
EP1049744A1 (en) 2000-11-08
JP2004323839A (ja) 2004-11-18
US6348596B1 (en) 2002-02-19
EP1049744B1 (en) 2002-01-23
AU2238399A (en) 1999-08-09
US6080868A (en) 2000-06-27
US20030207305A1 (en) 2003-11-06
US6541618B1 (en) 2003-04-01
DE69900813D1 (de) 2002-03-14

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