DE69900813D1 - Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher - Google Patents

Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher

Info

Publication number
DE69900813D1
DE69900813D1 DE69900813T DE69900813T DE69900813D1 DE 69900813 D1 DE69900813 D1 DE 69900813D1 DE 69900813 T DE69900813 T DE 69900813T DE 69900813 T DE69900813 T DE 69900813T DE 69900813 D1 DE69900813 D1 DE 69900813D1
Authority
DE
Germany
Prior art keywords
asymmetric cyanine
cyanine dyes
sub
dye
extinguishers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69900813T
Other languages
English (en)
Other versions
DE69900813T2 (de
Inventor
G Lee
J Graham
B Mullah
T Haxo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PE Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PE Corp filed Critical PE Corp
Application granted granted Critical
Publication of DE69900813D1 publication Critical patent/DE69900813D1/de
Publication of DE69900813T2 publication Critical patent/DE69900813T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
DE69900813T 1998-01-23 1999-01-19 Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher Expired - Lifetime DE69900813T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/012,525 US6080868A (en) 1998-01-23 1998-01-23 Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
PCT/US1999/001163 WO1999037717A1 (en) 1998-01-23 1999-01-19 Asymmetric cyanine dye quenchers

Publications (2)

Publication Number Publication Date
DE69900813D1 true DE69900813D1 (de) 2002-03-14
DE69900813T2 DE69900813T2 (de) 2002-10-02

Family

ID=21755376

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69900813T Expired - Lifetime DE69900813T2 (de) 1998-01-23 1999-01-19 Asymmetrische cyaninfarbstoffe als fluoreszenzlöscher

Country Status (8)

Country Link
US (5) US6080868A (de)
EP (1) EP1049744B1 (de)
JP (2) JP3946443B2 (de)
AT (1) ATE212367T1 (de)
AU (1) AU740989B2 (de)
CA (1) CA2318160C (de)
DE (1) DE69900813T2 (de)
WO (1) WO1999037717A1 (de)

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6583168B1 (en) * 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
US7625705B2 (en) * 1999-10-29 2009-12-01 Hologic, Inc. Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3′ flap
US20070134686A1 (en) * 1999-10-29 2007-06-14 Stratagene California Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap
JP2001204497A (ja) * 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、ｄｎａハイブリダイゼーション法によるｄｎａ検出方法
TW572969B (en) * 2000-02-10 2004-01-21 Hayashibara Biochem Lab Trimethine cyanine dye, light absorbent, light-resistant improver and optical recording medium containing same, and process for producing same
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
GB0024351D0 (en) * 2000-10-04 2000-11-22 Amersham Pharm Biotech Uk Ltd Dye-labelled peptide and method
US6811979B2 (en) * 2000-10-11 2004-11-02 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
CA2439750A1 (en) * 2001-03-09 2002-09-19 Boston Probes, Inc. Methods, kits and compositions pertaining to combination oligomers and libraries for their preparation
JP4676654B2 (ja) * 2001-07-19 2011-04-27 富士フイルム株式会社 蛍光ヌクレオチド及びそれを用いた標識法
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US8323903B2 (en) * 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
AU2003256587A1 (en) * 2002-07-18 2004-02-09 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
ATE449103T1 (de) 2002-09-08 2009-12-15 Applera Corp Festphasengebundene pna-dimere und deren synthese
CA2498320A1 (en) 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
AU2004230569B2 (en) 2003-03-31 2007-12-06 F. Hoffmann-La Roche Ag Compositions and methods for detecting certain flaviviruses, including members of the Japanese encephalitis virus serogroup
US7052412B2 (en) * 2003-05-07 2006-05-30 Bonneau Michael D Load transfer arm for putter
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US7271265B2 (en) * 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
ATE458200T1 (de) * 2003-09-12 2010-03-15 Life Technologies Corp Anwendungen des resonanzenergietransfers zwischen terbium und gfp
US7282339B2 (en) * 2003-09-17 2007-10-16 Invitrogen Corporation Competitive immunoassay
US20050118619A1 (en) * 2003-09-22 2005-06-02 Wensheng Xia Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
WO2005056689A2 (en) * 2003-12-05 2005-06-23 Molecular Probes, Inc. Cyanine dye compounds
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
US8883486B2 (en) 2004-05-04 2014-11-11 Universite De Montreal Arrestin biosensor
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20060292649A1 (en) * 2004-07-19 2006-12-28 Cell Biosciences Inc. Methods and apparatus for reference lab diagnostics
US7846676B2 (en) * 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US20060292558A1 (en) * 2004-07-19 2006-12-28 Cell Biosciences Inc. Methods and apparatus for protein assay diagnostics
WO2006014680A1 (en) 2004-07-19 2006-02-09 Cell Biosciences, Inc. Methods and devices for analyte detection
EP1781675B1 (de) 2004-08-13 2014-03-26 Epoch Biosciences, Inc. Fluoreszierende phosphonatfarbstoffe und konjugate
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
US7910753B2 (en) 2004-09-10 2011-03-22 Anaspec Incorporated Cyanine dyes and their applications as luminescence quenching compounds
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
US20060140203A1 (en) * 2004-12-28 2006-06-29 Sanjeev Jain System and method for packet queuing
US20070015176A1 (en) * 2005-02-18 2007-01-18 Applera Corporation Small nucleic acid detection probes and uses thereof
WO2006094200A2 (en) * 2005-03-03 2006-09-08 The Trustees Of Boston College Method of obtaining a desired localization for cellular imaging with the use of peptidoconjugates
US20070065948A1 (en) 2005-03-15 2007-03-22 Applera Corporation Use of antibody-surrogate antigen systems for detection of analytes
EP1872139A2 (de) * 2005-04-09 2008-01-02 Cell Biosciences, Inc. Automatisches mikrovolumen-assaysystem
EP1902142B1 (de) 2005-04-14 2010-01-06 Applied Biosystems, LLC 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden
EP1886145A2 (de) 2005-05-03 2008-02-13 Applera Corporation Fluoreszenznachweissystem und farbstoffsatz zur verwendung damit
JP5306811B2 (ja) * 2005-05-11 2013-10-02 ライフ テクノロジーズ コーポレーション ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
US20060286378A1 (en) * 2005-05-23 2006-12-21 Shivkumar Chiruvolu Nanostructured composite particles and corresponding processes
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US20060292574A1 (en) * 2005-05-26 2006-12-28 Center for Disease Control, Department of Health, Taiwan, R.O.C. Automated VNTR genotyping method
US8227621B2 (en) 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7630849B2 (en) * 2005-09-01 2009-12-08 Applied Biosystems, Llc Method of automated calibration and diagnosis of laboratory instruments
CN100361990C (zh) * 2005-09-06 2008-01-16 武汉大学 含吲哚基团的二阶非线性光学发色团及其制法
WO2007035864A2 (en) * 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
AU2005234696B2 (en) * 2005-11-18 2012-02-16 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
US8092784B2 (en) 2005-11-30 2012-01-10 Biotium, Inc. Enzyme substrate comprising a functional dye and associated technology and methods
WO2007106690A2 (en) 2006-03-15 2007-09-20 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
WO2008029295A2 (en) 2006-06-30 2008-03-13 Rosetta Genomics Ltd A method for detecting nucleic acids
US8425861B2 (en) * 2007-04-04 2013-04-23 Netbio, Inc. Methods for rapid multiplexed amplification of target nucleic acids
CN101343420B (zh) * 2007-07-12 2013-10-16 大连理工大学 一类不对称菁类荧光染料
US20090023156A1 (en) * 2007-07-20 2009-01-22 Voss Karl O Methods and reagents for quantifying analytes
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US8017338B2 (en) * 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
WO2009100382A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
US7943320B2 (en) * 2008-12-30 2011-05-17 Canon U.S. Life Sciences, Inc. Unsymmetrical cyanine dyes for high resolution nucleic acid melting analysis
US8332007B2 (en) * 2009-01-12 2012-12-11 The Board Of Trustees Of The University Of Illinois Quantitative three-dimensional mapping of oxygen tension
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2553123B1 (de) 2010-03-26 2016-08-24 Integrated DNA Technologies, Inc. Verfahren zur verstärkung einer nukleinsäurehybridisierung
CA2809457C (en) 2010-09-07 2019-07-30 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US8968875B2 (en) 2012-03-21 2015-03-03 Larry Takiff Eyewear including nitrophenyl functionalized boron pyrromethene dye for neutralizing laser threat
US8486311B1 (en) 2012-03-21 2013-07-16 Revision Military, Ltd. Eyewear including catechol functionalized boron pyrromethene dye for neutralizing laser threat
JP6240162B2 (ja) 2012-05-02 2017-11-29 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞収集システムおよびその使用
EP2769218B1 (de) 2012-05-02 2016-08-10 Charles River Laboratories, Inc. Lebensfähigkeitsfärbungsverfahren
JP6482459B2 (ja) 2012-05-02 2019-03-13 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞サンプル中の生存細胞を検出する方法
US10570463B2 (en) 2014-10-29 2020-02-25 Biotium, Inc. Nucleic acid modifying agents and uses thereof
NL2013786B1 (en) * 2014-11-13 2016-10-07 Original G B V Quenched coating.
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
JP6967006B2 (ja) 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析
CA3025031A1 (en) 2016-05-20 2017-11-23 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
EP3500684A4 (de) 2016-08-19 2020-02-26 Quantapore Inc. Optische nanoporensequenzierung mit quenchern
WO2018162986A2 (en) 2017-03-09 2018-09-13 Elitechgroup B.V. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
ES2909351T3 (es) 2017-08-17 2022-05-06 Elitechgroup Inc Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos
CN109053778A (zh) * 2018-06-14 2018-12-21 忻州师范学院 特异性检测氰根的试剂及其合成方法和应用
WO2020047477A1 (en) * 2018-08-31 2020-03-05 City Of Hope Cationic compounds for delivery of nucleic acids
US20230075533A1 (en) 2021-09-03 2023-03-09 ProteinSimple Methods and systems for analysis of samples containing particles used for gene delivery
CA3229091A1 (en) 2021-10-26 2023-05-04 Caribou Biosciences, Inc. Exonuclease-coupled real-time endonuclease activity assay

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2078233A (en) * 1934-09-14 1937-04-27 Eastman Kodak Co Photographic emulsion
GB455710A (en) * 1935-03-21 1936-10-21 Kodak Ltd Improvements in the manufacture of cyanine and related dyes
US2152615A (en) * 1936-08-29 1939-03-28 Agfa Ansco Corp Pentamethine-omega-aldehydes of heterocyclic bases
US2269234A (en) 1940-10-18 1942-01-06 Eastman Kodak Co Polymethine dye intermediates
US4230558A (en) 1978-10-02 1980-10-28 Coulter Electronics, Inc. Single drop separator
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4439356A (en) 1981-03-03 1984-03-27 Syva Company Unsymmetrical fluorescein derivatives
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5258538A (en) 1985-08-26 1993-11-02 Applied Biosystems, Inc. 2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0605655B1 (de) * 1991-09-16 1997-05-07 Molecular Probes, Inc. Dimere unsymmetrische Cyaninfarbstoffe
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
EP0601889A2 (de) 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5410030A (en) * 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
US5565554A (en) * 1994-07-29 1996-10-15 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
JP3189000B2 (ja) * 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US5658735A (en) * 1995-11-09 1997-08-19 Biometric Imaging, Inc. Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods
JPH09288326A (ja) * 1996-04-23 1997-11-04 Fuji Photo Film Co Ltd 熱現像感光材料
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
SE506700C2 (sv) * 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US5736333A (en) 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5770716A (en) 1997-04-10 1998-06-23 The Perkin-Elmer Corporation Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
WO2000075237A2 (en) 1999-06-09 2000-12-14 Carnegie Mellon University pH SENSITIVE CYANINE DYES AS REACTIVE FLUORESCENT REAGENTS

Also Published As

Publication number Publication date
EP1049744B1 (de) 2002-01-23
US20050009058A1 (en) 2005-01-13
US6541618B1 (en) 2003-04-01
JP2004323839A (ja) 2004-11-18
CA2318160C (en) 2005-11-29
ATE212367T1 (de) 2002-02-15
CA2318160A1 (en) 1999-07-29
DE69900813T2 (de) 2002-10-02
US6750024B2 (en) 2004-06-15
US6080868A (en) 2000-06-27
EP1049744A1 (de) 2000-11-08
JP2002501102A (ja) 2002-01-15
JP4471711B2 (ja) 2010-06-02
AU740989B2 (en) 2001-11-22
AU2238399A (en) 1999-08-09
JP3946443B2 (ja) 2007-07-18
US20030207305A1 (en) 2003-11-06
US7166715B2 (en) 2007-01-23
US6348596B1 (en) 2002-02-19
WO1999037717A1 (en) 1999-07-29

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