DE69836779T2 - Mutierte chimäre DNS-Polymerasen - Google Patents

Mutierte chimäre DNS-Polymerasen Download PDF

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Publication number
DE69836779T2
DE69836779T2 DE69836779T DE69836779T DE69836779T2 DE 69836779 T2 DE69836779 T2 DE 69836779T2 DE 69836779 T DE69836779 T DE 69836779T DE 69836779 T DE69836779 T DE 69836779T DE 69836779 T2 DE69836779 T2 DE 69836779T2
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DE
Germany
Prior art keywords
dna polymerase
dna
tma
amino acid
thermus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69836779T
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German (de)
English (en)
Other versions
DE69836779D1 (de
Inventor
Harrow David Oakland Gelfand
Lawrence Fred Oakland Reichert
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of DE69836779D1 publication Critical patent/DE69836779D1/de
Application granted granted Critical
Publication of DE69836779T2 publication Critical patent/DE69836779T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69836779T 1997-07-09 1998-07-03 Mutierte chimäre DNS-Polymerasen Expired - Lifetime DE69836779T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09
US52065P 1997-07-09

Publications (2)

Publication Number Publication Date
DE69836779D1 DE69836779D1 (de) 2007-02-15
DE69836779T2 true DE69836779T2 (de) 2007-04-26

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69836779T Expired - Lifetime DE69836779T2 (de) 1997-07-09 1998-07-03 Mutierte chimäre DNS-Polymerasen

Country Status (7)

Country Link
US (1) US6228628B1 (enExample)
EP (1) EP0892058B1 (enExample)
JP (1) JP4350815B2 (enExample)
AT (1) ATE325877T1 (enExample)
CA (1) CA2240570C (enExample)
DE (1) DE69836779T2 (enExample)
ES (1) ES2263188T3 (enExample)

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US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
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US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
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WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
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US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
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US9428737B1 (en) * 2010-01-11 2016-08-30 Eric Gaucher Taq polymerase sequences useful for incorporating analogs of nucleoside triphosphates
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
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EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
CN103492587B (zh) 2010-12-13 2015-02-18 生命技术公司 利用通过多磷酸分解作用(app)反应的活化聚合核酸
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US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
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WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
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JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
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ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ． ホフマン－ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ
JP7515413B2 (ja) 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
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JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN115772533B (zh) * 2022-08-24 2025-06-06 江南大学 一种细菌连续进化系统、正交易错dna聚合酶及连续进化方法
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Also Published As

Publication number Publication date
JP4350815B2 (ja) 2009-10-21
JPH1189588A (ja) 1999-04-06
EP0892058A2 (en) 1999-01-20
ATE325877T1 (de) 2006-06-15
CA2240570A1 (en) 1999-01-09
US6228628B1 (en) 2001-05-08
DE69836779D1 (de) 2007-02-15
ES2263188T3 (es) 2006-12-01
EP0892058A3 (en) 2001-09-26
EP0892058B1 (en) 2006-05-10
CA2240570C (en) 2009-12-15

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