DE69836779T2 - Mutierte chimäre DNS-Polymerasen - Google Patents

Mutierte chimäre DNS-Polymerasen Download PDF

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Publication number
DE69836779T2
DE69836779T2 DE69836779T DE69836779T DE69836779T2 DE 69836779 T2 DE69836779 T2 DE 69836779T2 DE 69836779 T DE69836779 T DE 69836779T DE 69836779 T DE69836779 T DE 69836779T DE 69836779 T2 DE69836779 T2 DE 69836779T2
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DE
Germany
Prior art keywords
dna polymerase
dna
tma
amino acid
thermus
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE69836779T
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German (de)
English (en)
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DE69836779D1 (de
Inventor
Harrow David Oakland Gelfand
Lawrence Fred Oakland Reichert
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of DE69836779D1 publication Critical patent/DE69836779D1/de
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Publication of DE69836779T2 publication Critical patent/DE69836779T2/de
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Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69836779T 1997-07-09 1998-07-03 Mutierte chimäre DNS-Polymerasen Expired - Lifetime DE69836779T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09
US52065P 1997-07-09

Publications (2)

Publication Number Publication Date
DE69836779D1 DE69836779D1 (de) 2007-02-15
DE69836779T2 true DE69836779T2 (de) 2007-04-26

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69836779T Expired - Lifetime DE69836779T2 (de) 1997-07-09 1998-07-03 Mutierte chimäre DNS-Polymerasen

Country Status (7)

Country Link
US (1) US6228628B1 (enExample)
EP (1) EP0892058B1 (enExample)
JP (1) JP4350815B2 (enExample)
AT (1) ATE325877T1 (enExample)
CA (1) CA2240570C (enExample)
DE (1) DE69836779T2 (enExample)
ES (1) ES2263188T3 (enExample)

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Also Published As

Publication number Publication date
JPH1189588A (ja) 1999-04-06
ES2263188T3 (es) 2006-12-01
ATE325877T1 (de) 2006-06-15
EP0892058B1 (en) 2006-05-10
JP4350815B2 (ja) 2009-10-21
DE69836779D1 (de) 2007-02-15
CA2240570A1 (en) 1999-01-09
EP0892058A2 (en) 1999-01-20
EP0892058A3 (en) 2001-09-26
US6228628B1 (en) 2001-05-08
CA2240570C (en) 2009-12-15

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