ATE325877T1 - Mutierte chimäre dns-polymerasen - Google Patents

Mutierte chimäre dns-polymerasen

Info

Publication number
ATE325877T1
ATE325877T1 AT98112327T AT98112327T ATE325877T1 AT E325877 T1 ATE325877 T1 AT E325877T1 AT 98112327 T AT98112327 T AT 98112327T AT 98112327 T AT98112327 T AT 98112327T AT E325877 T1 ATE325877 T1 AT E325877T1
Authority
AT
Austria
Prior art keywords
dna polymerase
enzymes
thermostable dna
chimeric thermostable
mutant
Prior art date
Application number
AT98112327T
Other languages
German (de)
English (en)
Inventor
David Harrow Gelfand
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE325877T1 publication Critical patent/ATE325877T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98112327T 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen ATE325877T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09

Publications (1)

Publication Number Publication Date
ATE325877T1 true ATE325877T1 (de) 2006-06-15

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98112327T ATE325877T1 (de) 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen

Country Status (7)

Country Link
US (1) US6228628B1 (enExample)
EP (1) EP0892058B1 (enExample)
JP (1) JP4350815B2 (enExample)
AT (1) ATE325877T1 (enExample)
CA (1) CA2240570C (enExample)
DE (1) DE69836779T2 (enExample)
ES (1) ES2263188T3 (enExample)

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ES3049590T3 (en) 2012-11-02 2025-12-17 Life Technologies Corp Small rna capture, detection and quantification
CN105339505B (zh) 2013-03-12 2021-08-10 生命技术公司 基于通用报告体的基因分型方法和材料
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
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CN111556900A (zh) 2017-12-22 2020-08-18 赛默飞世尔科技波罗的海封闭股份公司 包括胺的聚合酶链反应组合物
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CN112703248B (zh) 2018-09-13 2025-03-25 豪夫迈·罗氏有限公司 具有改良链置换能力的突变型dna聚合酶
KR20210084349A (ko) 2018-10-31 2021-07-07 일루미나, 인코포레이티드 중합효소, 조성물, 및 사용 방법
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Also Published As

Publication number Publication date
JPH1189588A (ja) 1999-04-06
ES2263188T3 (es) 2006-12-01
EP0892058B1 (en) 2006-05-10
JP4350815B2 (ja) 2009-10-21
DE69836779D1 (de) 2007-02-15
DE69836779T2 (de) 2007-04-26
CA2240570A1 (en) 1999-01-09
EP0892058A2 (en) 1999-01-20
EP0892058A3 (en) 2001-09-26
US6228628B1 (en) 2001-05-08
CA2240570C (en) 2009-12-15

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