ATE325877T1 - Mutierte chimäre dns-polymerasen - Google Patents

Mutierte chimäre dns-polymerasen

Info

Publication number
ATE325877T1
ATE325877T1 AT98112327T AT98112327T ATE325877T1 AT E325877 T1 ATE325877 T1 AT E325877T1 AT 98112327 T AT98112327 T AT 98112327T AT 98112327 T AT98112327 T AT 98112327T AT E325877 T1 ATE325877 T1 AT E325877T1
Authority
AT
Austria
Prior art keywords
dna polymerase
enzymes
thermostable dna
chimeric thermostable
mutant
Prior art date
Application number
AT98112327T
Other languages
German (de)
English (en)
Inventor
David Harrow Gelfand
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE325877T1 publication Critical patent/ATE325877T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98112327T 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen ATE325877T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09

Publications (1)

Publication Number Publication Date
ATE325877T1 true ATE325877T1 (de) 2006-06-15

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98112327T ATE325877T1 (de) 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen

Country Status (7)

Country Link
US (1) US6228628B1 (enExample)
EP (1) EP0892058B1 (enExample)
JP (1) JP4350815B2 (enExample)
AT (1) ATE325877T1 (enExample)
CA (1) CA2240570C (enExample)
DE (1) DE69836779T2 (enExample)
ES (1) ES2263188T3 (enExample)

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US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
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CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
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EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
CN103492587B (zh) 2010-12-13 2015-02-18 生命技术公司 利用通过多磷酸分解作用(app)反应的活化聚合核酸
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
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US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
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WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
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WO2014165210A2 (en) 2013-03-12 2014-10-09 Life Technologies Corporation Universal reporter-based genotyping methods and materials
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
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ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ． ホフマン－ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ
JP7515413B2 (ja) 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
EP3891275A2 (en) 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN115772533B (zh) * 2022-08-24 2025-06-06 江南大学 一种细菌连续进化系统、正交易错dna聚合酶及连续进化方法
CN118460500B (zh) * 2024-04-28 2025-01-07 湖南艾科瑞生物工程有限公司 一种融合dna聚合酶及其制备方法与应用
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Also Published As

Publication number Publication date
JP4350815B2 (ja) 2009-10-21
JPH1189588A (ja) 1999-04-06
EP0892058A2 (en) 1999-01-20
CA2240570A1 (en) 1999-01-09
US6228628B1 (en) 2001-05-08
DE69836779D1 (de) 2007-02-15
ES2263188T3 (es) 2006-12-01
EP0892058A3 (en) 2001-09-26
EP0892058B1 (en) 2006-05-10
CA2240570C (en) 2009-12-15
DE69836779T2 (de) 2007-04-26

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