CA2240570C - Mutant chimeric dna polymerase - Google Patents
Mutant chimeric dna polymerase Download PDFInfo
- Publication number
- CA2240570C CA2240570C CA002240570A CA2240570A CA2240570C CA 2240570 C CA2240570 C CA 2240570C CA 002240570 A CA002240570 A CA 002240570A CA 2240570 A CA2240570 A CA 2240570A CA 2240570 C CA2240570 C CA 2240570C
- Authority
- CA
- Canada
- Prior art keywords
- dna polymerase
- leu
- seq
- glu
- ala
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
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| US7238505B2 (en) | 2000-10-04 | 2007-07-03 | Ahram Biosystems Inc. | Immobilized DNA polymerase |
| DE10049211A1 (de) * | 2000-10-05 | 2002-04-18 | Qiagen Gmbh | Thermostabile Polymerase aus Thermococcus pacificus |
| NZ525336A (en) * | 2000-10-20 | 2006-03-31 | Expression Diagnostics Inc | Leukocyte expression profiling |
| US7026121B1 (en) * | 2001-06-08 | 2006-04-11 | Expression Diagnostics, Inc. | Methods and compositions for diagnosing and monitoring transplant rejection |
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| US6905827B2 (en) * | 2001-06-08 | 2005-06-14 | Expression Diagnostics, Inc. | Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases |
| US20050042629A1 (en) * | 2002-09-13 | 2005-02-24 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
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| US7052877B2 (en) | 2001-11-30 | 2006-05-30 | Applera Corporation | Thermus brockianus nucleic acid polymerases |
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| US7645575B2 (en) * | 2004-09-08 | 2010-01-12 | Xdx, Inc. | Genes useful for diagnosing and monitoring inflammation related disorders |
| US20060223067A1 (en) * | 2005-03-31 | 2006-10-05 | Paolo Vatta | Mutant DNA polymerases and methods of use |
| US7378260B2 (en) * | 2005-04-01 | 2008-05-27 | Applera Corporation | Products and methods for reducing dye artifacts |
| EP1885889A4 (en) * | 2005-05-11 | 2010-01-20 | Expression Diagnostics Inc | METHOD FOR MONITORING THE OPERATING STATUS OF TRANSPLANTS OVER GENLISTS |
| US20060292578A1 (en) | 2005-06-28 | 2006-12-28 | Weidong Zheng | DNA polymerase blends and mutant DNA polymerases |
| US20070190538A1 (en) * | 2005-07-29 | 2007-08-16 | Applera Corporation | Chimeric polymerases |
| EP2007909A4 (en) * | 2006-04-07 | 2011-01-26 | Xdx Inc | EXPRESSION OF NUCLEIC ACID IN RESPONSE TO STEROIDS AND PREDICTION OF DISEASE ACTIVITY |
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| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| WO2008140484A2 (en) * | 2006-11-09 | 2008-11-20 | Xdx, Inc. | Methods for diagnosing and monitoring the status of systemic lupus erythematosus |
| US8540517B2 (en) * | 2006-11-27 | 2013-09-24 | Pharos Innovations, Llc | Calculating a behavioral path based on a statistical profile |
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| US9428737B1 (en) * | 2010-01-11 | 2016-08-30 | Eric Gaucher | Taq polymerase sequences useful for incorporating analogs of nucleoside triphosphates |
| EP2582808B1 (en) | 2010-06-18 | 2015-04-22 | Roche Diagniostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| JP5876477B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
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| JP5926247B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
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| EP3161154B1 (en) * | 2014-06-27 | 2020-04-15 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| CN107429296B (zh) | 2015-03-13 | 2022-01-28 | 生命技术公司 | 捕获、检测和定量小rna的方法、组合物与试剂盒 |
| CN111556900A (zh) | 2017-12-22 | 2020-08-18 | 赛默飞世尔科技波罗的海封闭股份公司 | 包括胺的聚合酶链反应组合物 |
| ES2969883T3 (es) | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| CN112703248B (zh) | 2018-09-13 | 2025-03-25 | 豪夫迈·罗氏有限公司 | 具有改良链置换能力的突变型dna聚合酶 |
| KR20210084349A (ko) | 2018-10-31 | 2021-07-07 | 일루미나, 인코포레이티드 | 중합효소, 조성물, 및 사용 방법 |
| SG11202012559SA (en) | 2018-12-05 | 2021-01-28 | Illumina Inc | Polymerases, compositions, and methods of use |
| JP7209980B2 (ja) * | 2020-12-11 | 2023-01-23 | 東洋紡株式会社 | Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体 |
| US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| CN115772533B (zh) * | 2022-08-24 | 2025-06-06 | 江南大学 | 一种细菌连续进化系统、正交易错dna聚合酶及连续进化方法 |
| CN118460500B (zh) * | 2024-04-28 | 2025-01-07 | 湖南艾科瑞生物工程有限公司 | 一种融合dna聚合酶及其制备方法与应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| DK0550687T3 (da) * | 1990-09-28 | 1999-12-13 | Hoffmann La Roche | 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser |
| US5474920A (en) | 1993-11-23 | 1995-12-12 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Modified thermo-resistant DNA polymerases |
| US5912155A (en) * | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| EP0655506B1 (en) * | 1994-10-17 | 1996-09-18 | President And Fellows Of Harvard College | DNA polymerases having modified nucleotide binding site for DNA sequencing |
| US5614365A (en) | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5948614A (en) * | 1995-09-08 | 1999-09-07 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga maritima and mutants thereof |
-
1998
- 1998-06-26 US US09/105,697 patent/US6228628B1/en not_active Expired - Lifetime
- 1998-07-03 EP EP98112327A patent/EP0892058B1/en not_active Expired - Lifetime
- 1998-07-03 AT AT98112327T patent/ATE325877T1/de not_active IP Right Cessation
- 1998-07-03 ES ES98112327T patent/ES2263188T3/es not_active Expired - Lifetime
- 1998-07-03 DE DE69836779T patent/DE69836779T2/de not_active Expired - Lifetime
- 1998-07-07 CA CA002240570A patent/CA2240570C/en not_active Expired - Fee Related
- 1998-07-09 JP JP20853398A patent/JP4350815B2/ja not_active Expired - Fee Related
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|---|---|
| JPH1189588A (ja) | 1999-04-06 |
| ES2263188T3 (es) | 2006-12-01 |
| ATE325877T1 (de) | 2006-06-15 |
| EP0892058B1 (en) | 2006-05-10 |
| JP4350815B2 (ja) | 2009-10-21 |
| DE69836779D1 (de) | 2007-02-15 |
| DE69836779T2 (de) | 2007-04-26 |
| CA2240570A1 (en) | 1999-01-09 |
| EP0892058A2 (en) | 1999-01-20 |
| EP0892058A3 (en) | 2001-09-26 |
| US6228628B1 (en) | 2001-05-08 |
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