JP4350815B2 - 変性キメラｄｎａポリメラーゼ - Google Patents

変性キメラｄｎａポリメラーゼ Download PDF

Info

Publication number
JP4350815B2
JP4350815B2 JP20853398A JP20853398A JP4350815B2 JP 4350815 B2 JP4350815 B2 JP 4350815B2 JP 20853398 A JP20853398 A JP 20853398A JP 20853398 A JP20853398 A JP 20853398A JP 4350815 B2 JP4350815 B2 JP 4350815B2
Authority
JP
Japan
Prior art keywords
dna polymerase
dna
amino acid
sequence
tma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP20853398A
Other languages
English (en)
Japanese (ja)
Other versions
JPH1189588A (ja
JPH1189588A5 (enExample
Inventor
ハーロウ ゲルファンド デビッド
ローレンス レイチャート フレッド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JPH1189588A publication Critical patent/JPH1189588A/ja
Publication of JPH1189588A5 publication Critical patent/JPH1189588A5/ja
Application granted granted Critical
Publication of JP4350815B2 publication Critical patent/JP4350815B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP20853398A 1997-07-09 1998-07-09 変性キメラｄｎａポリメラーゼ Expired - Fee Related JP4350815B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09
US60/052065 1997-07-09

Publications (3)

Publication Number Publication Date
JPH1189588A JPH1189588A (ja) 1999-04-06
JPH1189588A5 JPH1189588A5 (enExample) 2005-10-13
JP4350815B2 true JP4350815B2 (ja) 2009-10-21

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20853398A Expired - Fee Related JP4350815B2 (ja) 1997-07-09 1998-07-09 変性キメラｄｎａポリメラーゼ

Country Status (7)

Country Link
US (1) US6228628B1 (enExample)
EP (1) EP0892058B1 (enExample)
JP (1) JP4350815B2 (enExample)
AT (1) ATE325877T1 (enExample)
CA (1) CA2240570C (enExample)
DE (1) DE69836779T2 (enExample)
ES (1) ES2263188T3 (enExample)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
AU4174397A (en) 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
GB9818432D0 (en) * 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
WO2001061015A2 (en) * 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AU2001241621B2 (en) 2000-02-23 2005-08-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination
EP1294863B1 (en) * 2000-05-24 2009-07-08 Third Wave Technologies, Inc. Detection of rna
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
WO2002004022A1 (en) * 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof
US7238505B2 (en) 2000-10-04 2007-07-03 Ahram Biosystems Inc. Immobilized DNA polymerase
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
CA2426540A1 (en) * 2000-10-20 2002-07-25 Biocardia, Inc. Leukocyte expression profiling
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
ATE406456T1 (de) 2001-11-28 2008-09-15 Bio Rad Laboratories Verfahren zur verwendung verbesserter polymerasen
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US7666645B2 (en) * 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
GB2416538B (en) * 2004-07-26 2008-07-30 Bioline Ltd Chimeric DNA polymerase
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20060223067A1 (en) * 2005-03-31 2006-10-05 Paolo Vatta Mutant DNA polymerases and methods of use
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
WO2006122295A2 (en) * 2005-05-11 2006-11-16 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
WO2007016702A2 (en) * 2005-07-29 2007-02-08 Applera Corporation Chimeric polymerases
AU2007309726A1 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2102367A2 (en) * 2006-11-09 2009-09-23 XDX, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US8540517B2 (en) * 2006-11-27 2013-09-24 Pharos Innovations, Llc Calculating a behavioral path based on a statistical profile
WO2009068268A1 (en) * 2007-11-28 2009-06-04 Roche Diagnostics Gmbh Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9428737B1 (en) * 2010-01-11 2016-08-30 Eric Gaucher Taq polymerase sequences useful for incorporating analogs of nucleoside triphosphates
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
CN103492587B (zh) 2010-12-13 2015-02-18 生命技术公司 利用通过多磷酸分解作用(app)反应的活化聚合核酸
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP3461807B1 (en) 2011-06-08 2023-07-12 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
WO2014165210A2 (en) 2013-03-12 2014-10-09 Life Technologies Corporation Universal reporter-based genotyping methods and materials
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
WO2015200693A1 (en) * 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
CN114250278B (zh) 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
WO2019122200A1 (en) 2017-12-22 2019-06-27 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ． ホフマン－ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ
JP7515413B2 (ja) 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
EP3891275A2 (en) 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN115772533B (zh) * 2022-08-24 2025-06-06 江南大学 一种细菌连续进化系统、正交易错dna聚合酶及连续进化方法
CN118460500B (zh) * 2024-04-28 2025-01-07 湖南艾科瑞生物工程有限公司 一种融合dna聚合酶及其制备方法与应用
WO2026153862A1 (en) 2025-01-14 2026-07-23 F. Hoffmann-La Roche Ag Dna polymerases with improved activity

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US5474920A (en) 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE69400567T2 (de) * 1994-10-17 1997-02-06 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit veränderter Nukleotid-Bindungstelle
AU7236296A (en) * 1995-09-08 1997-03-27 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof

Also Published As

Publication number Publication date
JPH1189588A (ja) 1999-04-06
EP0892058A2 (en) 1999-01-20
ATE325877T1 (de) 2006-06-15
CA2240570A1 (en) 1999-01-09
US6228628B1 (en) 2001-05-08
DE69836779D1 (de) 2007-02-15
ES2263188T3 (es) 2006-12-01
EP0892058A3 (en) 2001-09-26
EP0892058B1 (en) 2006-05-10
CA2240570C (en) 2009-12-15
DE69836779T2 (de) 2007-04-26

Similar Documents

Publication Publication Date Title
EP0892058B1 (en) Mutant chimeric DNA polymerases
EP2272952B1 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
US5968799A (en) Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
US5489523A (en) Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5624833A (en) Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima
EP0550687B1 (en) 5' to 3' exonuclease mutations of thermostable dna polymerases
US5405774A (en) DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5466591A (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5455170A (en) Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5545552A (en) Purified thermostable pyrococcus furiosus DNA polymerase I
US5795762A (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
US7045328B2 (en) Purified thermostable Pyrococcus furiosus DNA polymerase I
US5814506A (en) Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion
JP2000501616A (ja) テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性ｄｎａポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素
CA2280001A1 (en) Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1992009689A1 (en) PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)
US20020076768A1 (en) Modified thermostable DNA polymerase
CA2185362C (en) Purified nucleic acid encoding a thermostable pyrophosphatase
US5866395A (en) Purified thermostable pyrococcus furiosus DNA polymerase I
WO2003048310A2 (en) Thermus oshimai nucleic acid polymerases

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20050531

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20050531

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20080520

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080819

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20081125

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090225

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20090623

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090723

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120731

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130731

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees