DE69738873D1 - Mutierte penicillin g acylasen - Google Patents

Mutierte penicillin g acylasen

Info

Publication number
DE69738873D1
DE69738873D1 DE69738873T DE69738873T DE69738873D1 DE 69738873 D1 DE69738873 D1 DE 69738873D1 DE 69738873 T DE69738873 T DE 69738873T DE 69738873 T DE69738873 T DE 69738873T DE 69738873 D1 DE69738873 D1 DE 69738873D1
Authority
DE
Germany
Prior art keywords
penicillin
mutaten
acylasen
acylases
acylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738873T
Other languages
English (en)
Inventor
Li You
John J Usher
Brenda J White
Jiri Novotny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Application granted granted Critical
Publication of DE69738873D1 publication Critical patent/DE69738873D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69738873T 1996-11-05 1997-10-31 Mutierte penicillin g acylasen Expired - Lifetime DE69738873D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3036596P 1996-11-05 1996-11-05
PCT/US1997/020298 WO1998020120A1 (en) 1996-11-05 1997-10-31 Mutant penicillin g acylases

Publications (1)

Publication Number Publication Date
DE69738873D1 true DE69738873D1 (de) 2008-09-11

Family

ID=21853878

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69738873T Expired - Lifetime DE69738873D1 (de) 1996-11-05 1997-10-31 Mutierte penicillin g acylasen

Country Status (12)

Country Link
US (1) US6403356B1 (de)
EP (1) EP0961825B1 (de)
JP (1) JP4080542B2 (de)
KR (1) KR100570244B1 (de)
AT (1) ATE402997T1 (de)
AU (1) AU718648B2 (de)
CA (1) CA2270400C (de)
DE (1) DE69738873D1 (de)
HK (1) HK1024933A1 (de)
HU (1) HU226348B1 (de)
IL (1) IL129055A (de)
WO (1) WO1998020120A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891703A (en) * 1994-08-12 1999-04-06 Gist-Hrocades Mutated penicillin G acylase genes
JP4080542B2 (ja) 1996-11-05 2008-04-23 ブリストル―マイヤーズ・スクイブ・カンパニー 突然変異ペニシリンｇアシラーゼ
WO2000066751A1 (en) * 1999-04-29 2000-11-09 Dsm N.V. Expression cassette for efficient production of a protein
KR20040075042A (ko) * 2001-12-27 2004-08-26 디에스엠 아이피 어셋츠 비.브이. β-락탐 항생제의 제조 방법
ITMI20030042A1 (it) * 2003-01-14 2004-07-15 Univ Degli Studi Trieste Processo di sintesi enzimatica di antibiotici b lattamici.
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
US8071330B2 (en) 2004-12-27 2011-12-06 Dsm Ip Assets B.V. Process for the synthesis of cefaclor
CN101177688B (zh) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株
EP2173892B8 (de) 2007-07-27 2012-08-01 Fermenta Biotech Limited Verfahren zur Präparation von immobilisierter rekombinanter Penicillin Acylase aus Achromobacter sp CCM 4824 exprimiert in E. coli BL21 CCM 7394 und dessen Verwendung für die Synthese von beta-Lactam Antibiotika
EP2367940B1 (de) * 2008-12-23 2017-11-22 DSM Sinochem Pharmaceuticals Netherlands B.V. Mutierte Penicillin-G-Acylasen
EP2513327B1 (de) 2009-12-14 2016-06-29 DSM Sinochem Pharmaceuticals Netherlands B.V. Herstellungsverfahren für cephradin
WO2011113486A1 (en) 2010-03-17 2011-09-22 Deretil, S.A. Process for the synthesis of hydroxyphenylglycine esters
RU2564578C2 (ru) * 2012-04-25 2015-10-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) МУТАНТ ПЕНИЦИЛЛИНАЦИЛАЗЫ ИЗ E.coli С УЛУЧШЕННЫМИ СВОЙСТВАМИ
RU2537845C2 (ru) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы
RU2576002C2 (ru) * 2013-10-18 2016-02-27 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ улучшения каталитических свойств пенициллинацилазы из escherichia coli и применение мутантной пенициллинацилазы
CN103667418B (zh) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 高活性β-内酰胺抗生素合成用酶的高通量筛选方法
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
US10724025B2 (en) * 2016-05-05 2020-07-28 Codexis, Inc. Penicillin-G acylases
KR101985911B1 (ko) * 2017-12-28 2019-06-04 아미코젠주식회사 Achromobacter sp. CCM 4824 유래 페니실린 G 아실라제 변이체 및 이의 이용
KR102363768B1 (ko) * 2019-11-15 2022-02-16 아미코젠주식회사 세파졸린 생산성이 증가된 페니실린 g 아실라제 변이체 및 이의 이용
CN115418331B (zh) * 2022-08-29 2024-09-10 新发药业有限公司 一种产青霉素g酰基转移酶自诱导培养基及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554250A (en) * 1983-06-30 1985-11-19 E. R. Squibb & Sons, Inc. Method of increased production of penicillin acylase and plasmid employed therein
JPS6121097A (ja) 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
SE8403929L (sv) 1984-07-31 1986-02-21 Sten Gatenbeck Rekombinant-dna-molekyl, transformerade mikroorganismer och forfarande for framstellning av penicillin v-amidas
DE3439843A1 (de) * 1984-10-31 1986-04-30 Boehringer Mannheim Gmbh, 6800 Mannheim Plasmide zur erhoehten produktion von penicillin g-amidase
US4981789A (en) 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (de) 1987-12-21 1991-01-30 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt
US5229274A (en) * 1989-06-27 1993-07-20 Merck & Co., Inc. Gene encoding one step cephalosporin C amidase and expression thereof in recombinant bacillus
DE69129604T2 (de) 1990-04-18 1998-10-15 Gist Brocades Nv Mutierte beta-Lactamacylasegene
ATE124724T1 (de) * 1990-04-18 1995-07-15 Gist Brocades Nv Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym.
GB9019724D0 (en) 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
US5891703A (en) * 1994-08-12 1999-04-06 Gist-Hrocades Mutated penicillin G acylase genes
US5516679A (en) 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
JP4080542B2 (ja) 1996-11-05 2008-04-23 ブリストル―マイヤーズ・スクイブ・カンパニー 突然変異ペニシリンｇアシラーゼ

Also Published As

Publication number Publication date
HU226348B1 (en) 2008-09-29
ATE402997T1 (de) 2008-08-15
HUP9904162A3 (en) 2001-06-28
KR20000053041A (ko) 2000-08-25
AU718648B2 (en) 2000-04-20
CA2270400C (en) 2010-09-28
EP0961825A1 (de) 1999-12-08
HK1024933A1 (en) 2000-10-27
EP0961825A4 (de) 2002-11-20
JP2001503272A (ja) 2001-03-13
KR100570244B1 (ko) 2006-04-12
WO1998020120A1 (en) 1998-05-14
AU5248698A (en) 1998-05-29
HUP9904162A2 (hu) 2000-03-28
CA2270400A1 (en) 1998-05-14
US6403356B1 (en) 2002-06-11
JP4080542B2 (ja) 2008-04-23
IL129055A (en) 2005-06-19
IL129055A0 (en) 2000-02-17
EP0961825B1 (de) 2008-07-30

Similar Documents

Publication Publication Date Title
DE69738873D1 (de) Mutierte penicillin g acylasen
ES2144139T3 (es) Genes de la penicilina g-acilasa mutados.
NO20021931L (no) L-pantolacton-hydrolase og en fremgangsmåte for fremstilling av D-pantolacton
DE69129604D1 (de) Mutierte beta-Lactamacylasegene
DK0652960T3 (da) Cyklisk GMP-bindende, cyklisk GMP-specifikke phosphodiesterasematerialer og fremgangsmåder
CA2166685A1 (en) An enzyme with protease activity
IE890446L (en) Novel proteolytic enzymes and their use in detergents
DK1106696T3 (da) Proteindeamiderende enzym, mikroorganisme producerende dette, gen kodende derfor, fremgangsmåde til fremstilling af enzymet og anvendelse deraf
PT1165763E (pt) Variantes de hidantoinase com propriedades melhoradas e a sua utilizacao para a producao de aminoacidos
FI962247A (fi) Uudet apoptoosia muuntavat proteiinit, niitä koodaava DNA ja näiden käyttömenetelmät
DK0981631T3 (da) Polypeptider, som har aminopeptidase-aktivitet, og nukleinsyrer, som koder for samme
HUP0202596A2 (hu) Szignálszekvenciák Leu-hirudin előállítására E. coli általi szekréció révén tenyésztő tápközegben
EP1757684A3 (de) 2,6-Beta-D-Fruktanhydrolase Enzym und Verfahren zu dessen Verwendung
ATE248923T1 (de) Promtors für gen expression
EP1010761A4 (de) Gen
ATE356212T1 (de) O-fukosyltransferase
NO20020064D0 (no) Chlamysin B antibakterielt protein, gen som koder for dette samt et ekspresjonssystem for dette
DE69938427D1 (de) Tes gen und dessen verwendung
DK43393D0 (da) Dna-sekvens, der koder for et enzym
DK1141337T3 (da) Gram-positive bakterier, som mangler HtrA-proteaseaktivitet, samt deres anvendelser
EA200100440A1 (ru) Ген prv-1 и его применение
HUP0400915A2 (hu) Növényi reprodukciós proteinek
ATE28186T1 (de) Alpha-allenyl-alpha-amino carbonsaeuren.
AU7416100A (en) Dihydroorotase extracted from plants

Legal Events

Date Code Title Description
8364 No opposition during term of opposition