ES2144139T3 - Genes de la penicilina g-acilasa mutados. - Google Patents
Genes de la penicilina g-acilasa mutados.Info
- Publication number
- ES2144139T3 ES2144139T3 ES95929882T ES95929882T ES2144139T3 ES 2144139 T3 ES2144139 T3 ES 2144139T3 ES 95929882 T ES95929882 T ES 95929882T ES 95929882 T ES95929882 T ES 95929882T ES 2144139 T3 ES2144139 T3 ES 2144139T3
- Authority
- ES
- Spain
- Prior art keywords
- penicillin
- acilase
- mutated
- genes
- acylases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
- C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/84—Penicillin amidase (3.5.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE PROPORCIONAN NUEVOS MUTANTES BE -LACTAMA DE ACILASA DE PENICILINA G, QUE PRESENTAN ESPECIFICIDADES DE SUSTRATO ALTERADAS. DICHAS ACILASAS DE PENICILINA G, SE OBTIENEN MEDIANTE EXPRESION DE UN GEN QUE CODIFICA PARA DICHA ACILASA DE PENICILINA G, LA CUAL TIENE UNA SECUENCIA AMINOACIDA QUE DIFIERE AL MENOS EN UN AMINOACIDO DE LA ACILASA DE PENICILINA G DE TIPO SILVESTRE.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP94202314 | 1994-08-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2144139T3 true ES2144139T3 (es) | 2000-06-01 |
Family
ID=8217100
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95929882T Expired - Lifetime ES2144139T3 (es) | 1994-08-12 | 1995-08-14 | Genes de la penicilina g-acilasa mutados. |
Country Status (10)
Country | Link |
---|---|
US (2) | US5891703A (es) |
EP (1) | EP0775210B1 (es) |
JP (1) | JP4147319B2 (es) |
CN (1) | CN1159444C (es) |
AT (1) | ATE189477T1 (es) |
AU (1) | AU3346295A (es) |
DE (1) | DE69514923T2 (es) |
ES (1) | ES2144139T3 (es) |
MX (1) | MX9701025A (es) |
WO (1) | WO1996005318A1 (es) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5891703A (en) * | 1994-08-12 | 1999-04-06 | Gist-Hrocades | Mutated penicillin G acylase genes |
ATE402997T1 (de) * | 1996-11-05 | 2008-08-15 | Bristol Myers Squibb Co | Mutierte penicillin g acylasen |
US6897058B2 (en) * | 2000-09-08 | 2005-05-24 | Dsm Ip Assets B.V. | Method for the preparation of enantiomerically enriched amines |
AU2002358777A1 (en) * | 2001-12-27 | 2003-07-15 | Dsm N.V. | Process for the preparation of a beta-lactam antibiotic with mutated penicillin acylase |
JP4113427B2 (ja) * | 2002-08-16 | 2008-07-09 | 富士フイルム株式会社 | 熱現像感光材料 |
DE10251747A1 (de) * | 2002-11-05 | 2004-05-19 | Tuhh-Technologie-Gmbh | Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung |
WO2004111241A1 (en) * | 2003-06-12 | 2004-12-23 | Stichting Voor De Technische Wetenschappen | Penicillin acylase |
KR100530299B1 (ko) * | 2003-08-11 | 2005-11-22 | 산도즈 게엠베하 | 변이 세팔로스포린 c 아실라제 및 이를 이용한 7-aca 제조방법 |
ITMI20040016A1 (it) * | 2004-01-12 | 2004-04-12 | Antibiotics S P A | Cefalosporina c acilasi |
CN101177688B (zh) * | 2006-11-08 | 2010-12-01 | 中国科学院上海生命科学研究院 | 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株 |
CZ200782A3 (cs) * | 2007-01-31 | 2009-05-27 | Mikrobiologický ústav AV CR, v.v.i. | Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci |
US8288141B2 (en) * | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
WO2010027710A2 (en) * | 2008-08-27 | 2010-03-11 | Codexis, Inc. | Ketoreductase polypeptides and uses thereof |
US8426178B2 (en) * | 2008-08-27 | 2013-04-23 | Codexis, Inc. | Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
EP2329014B1 (en) | 2008-08-29 | 2014-10-22 | Codexis, Inc. | Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one |
US8247192B2 (en) | 2008-11-10 | 2012-08-21 | Codexis, Inc. | Penicillin-G acylases |
EP2367940B1 (en) * | 2008-12-23 | 2017-11-22 | DSM Sinochem Pharmaceuticals Netherlands B.V. | Mutant penicillin G acylases |
EP2467473B1 (en) | 2009-08-19 | 2016-03-23 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
HUE026367T2 (en) | 2010-05-04 | 2016-06-28 | Codexis Inc | Biocatalysts of ezetimibe synthesis |
WO2012175585A1 (en) | 2011-06-23 | 2012-12-27 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for preparing 3'-thiosubstituted cephalosporins employing a penicillin g acylase |
EP2723881A2 (en) | 2011-06-23 | 2014-04-30 | DSM Sinochem Pharmaceuticals Netherlands B.V. | Novel crystalline cefoperazone intermediate |
WO2013057197A1 (en) | 2011-10-20 | 2013-04-25 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for the formylation of cefamandole |
WO2013057196A1 (en) | 2011-10-20 | 2013-04-25 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for the preparation of cefamandole nafate |
RU2537845C2 (ru) * | 2012-04-25 | 2015-01-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) | Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы |
CN103667418B (zh) * | 2013-12-18 | 2015-03-25 | 华北制药河北华民药业有限责任公司 | 高活性β-内酰胺抗生素合成用酶的高通量筛选方法 |
JP6379531B2 (ja) * | 2014-03-10 | 2018-08-29 | 東洋紡株式会社 | 新規β−アミノ酸の製造方法 |
SG11201708356PA (en) | 2015-05-07 | 2017-11-29 | Codexis Inc | Penicillin-g acylases |
WO2017186864A1 (en) | 2016-04-27 | 2017-11-02 | Sandoz Ag | Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum |
WO2019005337A1 (en) * | 2017-06-27 | 2019-01-03 | Codexis, Inc. | PENICILLIN G ACYLASES |
CN110343734B (zh) * | 2019-06-14 | 2021-03-23 | 浙江工业大学 | 一种l-草铵膦化学酶法生产方法 |
CN111172142B (zh) * | 2020-02-14 | 2021-09-28 | 上海陶宇晟生物技术有限责任公司 | 一种热稳定性高的头孢菌素c酰化酶突变体 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6121097A (ja) * | 1984-07-10 | 1986-01-29 | Asahi Chem Ind Co Ltd | 7−アミノセフアロスポラン酸及びその誘導体の製造法 |
US4981789A (en) * | 1987-03-18 | 1991-01-01 | Merck & Co., Inc. | One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives |
EP0322032A3 (en) * | 1987-12-21 | 1991-01-30 | Merck & Co. Inc. | One-step enzymatic conversion of cephalosporin c and derivatives to 7-aminocephalosporanic acid and derivatives |
EP0453048B1 (en) * | 1990-04-18 | 1998-06-17 | Gist-Brocades N.V. | Mutated beta-lactam acylase genes |
KR0152667B1 (ko) * | 1990-04-18 | 1998-10-01 | 한스 월터 라벤 | 페니실린 g 아실라아제, 이 효소를 코드화한 유전자 및 이 효소의 제조방법 |
GB9019724D0 (en) * | 1990-09-10 | 1990-10-24 | Fujisawa Pharmaceutical Co | Cephalosporin c acylase |
GB9204439D0 (en) * | 1992-02-27 | 1992-04-15 | Fujisawa Pharmaceutical Co | A new cephalosporin c acylase |
TW400384B (en) * | 1993-11-01 | 2000-08-01 | Fujisawa Pharmaceutical Co | A new cephalosporin C acylase |
US5891703A (en) * | 1994-08-12 | 1999-04-06 | Gist-Hrocades | Mutated penicillin G acylase genes |
US5516679A (en) * | 1994-12-23 | 1996-05-14 | Bristol-Myers Squibb Company | Penicillin V amidohydrolase gene from Fusarium oxysporum |
ATE402997T1 (de) * | 1996-11-05 | 2008-08-15 | Bristol Myers Squibb Co | Mutierte penicillin g acylasen |
-
1995
- 1995-08-14 US US08/793,229 patent/US5891703A/en not_active Expired - Lifetime
- 1995-08-14 JP JP50703396A patent/JP4147319B2/ja not_active Expired - Fee Related
- 1995-08-14 AT AT95929882T patent/ATE189477T1/de active
- 1995-08-14 DE DE69514923T patent/DE69514923T2/de not_active Expired - Fee Related
- 1995-08-14 CN CNB951952269A patent/CN1159444C/zh not_active Expired - Lifetime
- 1995-08-14 ES ES95929882T patent/ES2144139T3/es not_active Expired - Lifetime
- 1995-08-14 EP EP95929882A patent/EP0775210B1/en not_active Expired - Lifetime
- 1995-08-14 AU AU33462/95A patent/AU3346295A/en not_active Abandoned
- 1995-08-14 WO PCT/EP1995/003249 patent/WO1996005318A1/en active IP Right Grant
- 1995-08-14 MX MX9701025A patent/MX9701025A/es unknown
-
1999
- 1999-04-05 US US09/285,957 patent/US6033823A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CN1158638A (zh) | 1997-09-03 |
US6033823A (en) | 2000-03-07 |
ATE189477T1 (de) | 2000-02-15 |
JPH10507072A (ja) | 1998-07-14 |
DE69514923T2 (de) | 2000-11-02 |
AU3346295A (en) | 1996-03-07 |
JP4147319B2 (ja) | 2008-09-10 |
DE69514923D1 (de) | 2000-03-09 |
EP0775210A1 (en) | 1997-05-28 |
MX9701025A (es) | 1997-05-31 |
WO1996005318A1 (en) | 1996-02-22 |
EP0775210B1 (en) | 2000-02-02 |
US5891703A (en) | 1999-04-06 |
CN1159444C (zh) | 2004-07-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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