MX9701025A - Genes de penicilina g acilasa mutados. - Google Patents

Genes de penicilina g acilasa mutados.

Info

Publication number
MX9701025A
MX9701025A MX9701025A MX9701025A MX9701025A MX 9701025 A MX9701025 A MX 9701025A MX 9701025 A MX9701025 A MX 9701025A MX 9701025 A MX9701025 A MX 9701025A MX 9701025 A MX9701025 A MX 9701025A
Authority
MX
Mexico
Prior art keywords
penicillin
acylase
mutated penicillin
acylases
amino acid
Prior art date
Application number
MX9701025A
Other languages
English (en)
Inventor
Jan Metske Van Der Laan
Adriana Marina Riemens
Wilhelmus Johannes Quax
Original Assignee
Gist Brocades Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Bv filed Critical Gist Brocades Bv
Publication of MX9701025A publication Critical patent/MX9701025A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position

Abstract

Se describen nuevas Penicilina G acilasas de beta-lactama mutantes, que presentan especificaciones de sustrato alteradas. Estas Penicilina G acilasa son obtenidas por la expresion de un gen que codifica para la Penicilina G acilasa y que tiene una secuencia de aminoácidos, la cual difiere por lo menos en un aminoácido de la Penicilina G acilasa de tipo silvestre.
MX9701025A 1994-08-12 1995-08-14 Genes de penicilina g acilasa mutados. MX9701025A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94202314 1994-08-12
PCT/EP1995/003249 WO1996005318A1 (en) 1994-08-12 1995-08-14 Mutated penicillin g acylase genes

Publications (1)

Publication Number Publication Date
MX9701025A true MX9701025A (es) 1997-05-31

Family

ID=8217100

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9701025A MX9701025A (es) 1994-08-12 1995-08-14 Genes de penicilina g acilasa mutados.

Country Status (10)

Country Link
US (2) US5891703A (es)
EP (1) EP0775210B1 (es)
JP (1) JP4147319B2 (es)
CN (1) CN1159444C (es)
AT (1) ATE189477T1 (es)
AU (1) AU3346295A (es)
DE (1) DE69514923T2 (es)
ES (1) ES2144139T3 (es)
MX (1) MX9701025A (es)
WO (1) WO1996005318A1 (es)

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JP4147319B2 (ja) * 1994-08-12 2008-09-10 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 変異ペニシリンｇアシラーゼ遺伝子
US6403356B1 (en) * 1996-11-05 2002-06-11 Bristol-Myers Squibb Co. Mutant penicillin G acylases
JP2004508051A (ja) * 2000-09-08 2004-03-18 ディーエスエム　エヌ．ブイ． エナンチオマーに富むアミンの製造法
AU2002358777A1 (en) * 2001-12-27 2003-07-15 Dsm N.V. Process for the preparation of a beta-lactam antibiotic with mutated penicillin acylase
JP4113427B2 (ja) * 2002-08-16 2008-07-09 富士フイルム株式会社 熱現像感光材料
DE10251747A1 (de) * 2002-11-05 2004-05-19 Tuhh-Technologie-Gmbh Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung
WO2004111241A1 (en) * 2003-06-12 2004-12-23 Stichting Voor De Technische Wetenschappen Penicillin acylase
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CN101177688B (zh) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株
CZ300467B6 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
EP2329013B1 (en) * 2008-08-27 2015-10-28 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288141B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
SG10201404330VA (en) 2008-08-29 2014-10-30 Codexis Inc Ketoreductase Polypeptides For The Stereoselective Production Of (4S)-3[(5S)-5(4-Fluorophenyl)-5-Hydroxypentanoyl]-4-Phenyl-1,3-Oxazolidin-2-One
WO2010054319A2 (en) * 2008-11-10 2010-05-14 Codexis, Inc. Penicillin-g acylases
MX2011006802A (es) 2008-12-23 2011-07-20 Dsm Ip Assets Bv Acilasas de penicilina g mutante.
ES2575560T3 (es) 2009-08-19 2016-06-29 Codexis, Inc. Polipéptidos cetorreductasa para preparar fenilefrina
US9040262B2 (en) 2010-05-04 2015-05-26 Codexis, Inc. Biocatalysts for ezetimibe synthesis
PL2723882T3 (pl) 2011-06-23 2020-01-31 Centrient Pharmaceuticals Netherlands B.V. Sposób wytwarzania 3’-tiopodstawionych cefalosporyn z zastosowaniem acylazy penicyliny
EP2723881A2 (en) 2011-06-23 2014-04-30 DSM Sinochem Pharmaceuticals Netherlands B.V. Novel crystalline cefoperazone intermediate
WO2013057197A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the formylation of cefamandole
WO2013057196A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the preparation of cefamandole nafate
RU2537845C2 (ru) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы
CN103667418B (zh) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 高活性β-内酰胺抗生素合成用酶的高通量筛选方法
JP6379531B2 (ja) * 2014-03-10 2018-08-29 東洋紡株式会社 新規β−アミノ酸の製造方法
EP3292136B1 (en) 2015-05-07 2021-02-17 Codexis, Inc. Penicillin-g acylases
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
EP3645713A4 (en) * 2017-06-27 2021-06-30 Codexis, Inc. PENICILLIN G ACYLASES
CN110343734B (zh) * 2019-06-14 2021-03-23 浙江工业大学 一种l-草铵膦化学酶法生产方法
CN111172142B (zh) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体

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Publication number Priority date Publication date Assignee Title
JPS6121097A (ja) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
US4981789A (en) * 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (en) * 1987-12-21 1991-01-30 Merck & Co. Inc. One-step enzymatic conversion of cephalosporin c and derivatives to 7-aminocephalosporanic acid and derivatives
US5168048A (en) * 1990-04-18 1992-12-01 Gist-Brocades Nv Penicillin G acylase, a gene encoding the same and a method for the production of this enzyme
WO1991016435A1 (en) * 1990-04-18 1991-10-31 Gist-Brocades N.V. Mutated beta-lactam acylase genes
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
JP4147319B2 (ja) * 1994-08-12 2008-09-10 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 変異ペニシリンｇアシラーゼ遺伝子
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
US6403356B1 (en) * 1996-11-05 2002-06-11 Bristol-Myers Squibb Co. Mutant penicillin G acylases

Also Published As

Publication number Publication date
EP0775210B1 (en) 2000-02-02
US5891703A (en) 1999-04-06
AU3346295A (en) 1996-03-07
US6033823A (en) 2000-03-07
CN1158638A (zh) 1997-09-03
DE69514923T2 (de) 2000-11-02
WO1996005318A1 (en) 1996-02-22
EP0775210A1 (en) 1997-05-28
JPH10507072A (ja) 1998-07-14
ES2144139T3 (es) 2000-06-01
CN1159444C (zh) 2004-07-28
DE69514923D1 (de) 2000-03-09
ATE189477T1 (de) 2000-02-15
JP4147319B2 (ja) 2008-09-10

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