UA26870C2 - Спосіб підвищеhhя продукуваhhя протеїhу с, спосіб підвищеhhя рівhя його модифікації - Google Patents

Спосіб підвищеhhя продукуваhhя протеїhу с, спосіб підвищеhhя рівhя його модифікації

Info

Publication number
UA26870C2
UA26870C2 UA94129175A UA94129175A UA26870C2 UA 26870 C2 UA26870 C2 UA 26870C2 UA 94129175 A UA94129175 A UA 94129175A UA 94129175 A UA94129175 A UA 94129175A UA 26870 C2 UA26870 C2 UA 26870C2
Authority
UA
Ukraine
Prior art keywords
increasing
protein
production
cells
modification level
Prior art date
Application number
UA94129175A
Other languages
English (en)
Russian (ru)
Inventor
Брайан Уільям ГРІHHЕЛЛ
Original Assignee
Елі Ліллі Енд Компані
Эли Лилли Энд Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Елі Ліллі Енд Компані, Эли Лилли Энд Компани filed Critical Елі Ліллі Енд Компані
Publication of UA26870C2 publication Critical patent/UA26870C2/uk

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Abstract

Запропоновано спосіб підвищення продукування протеїну С при культивуванні аденовірустрансформованих рекомбінантних клітин ссавців. Культивування здійснюють при температурі близько 39 С, а згадані клітини вибирають з групи, що включає рекомбінантні клітини AV 12 та рекомбінантні клітини 293.
UA94129175A 1993-12-15 1994-12-14 Спосіб підвищеhhя продукуваhhя протеїhу с, спосіб підвищеhhя рівhя його модифікації UA26870C2 (uk)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (1)

Publication Number Publication Date
UA26870C2 true UA26870C2 (uk) 1999-12-29

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
UA94129175A UA26870C2 (uk) 1993-12-15 1994-12-14 Спосіб підвищеhhя продукуваhhя протеїhу с, спосіб підвищеhhя рівhя його модифікації

Country Status (28)

Country Link
US (1) US5618714A (uk)
EP (1) EP0658626B1 (uk)
JP (1) JP3662614B2 (uk)
KR (1) KR950018454A (uk)
CN (1) CN1107887A (uk)
AT (1) ATE196165T1 (uk)
AU (1) AU687914B2 (uk)
BR (1) BR9404991A (uk)
CA (1) CA2138101A1 (uk)
CO (1) CO4340654A1 (uk)
CZ (2) CZ315794A3 (uk)
DE (1) DE69425809T2 (uk)
DK (1) DK0658626T3 (uk)
ES (1) ES2149244T3 (uk)
FI (1) FI106385B (uk)
GR (1) GR3034909T3 (uk)
HU (1) HU219508B (uk)
IL (1) IL111983A0 (uk)
NO (1) NO944846L (uk)
NZ (1) NZ270140A (uk)
PE (1) PE42695A1 (uk)
PL (1) PL176381B1 (uk)
PT (1) PT658626E (uk)
RU (1) RU2110574C1 (uk)
SI (1) SI0658626T1 (uk)
UA (1) UA26870C2 (uk)
YU (1) YU73494A (uk)
ZA (1) ZA949981B (uk)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
CN100404673C (zh) 2001-02-19 2008-07-23 默克专利有限公司 鉴定t细胞表位的方法及制备具有降低的免疫原性的分子的用途
WO2003085138A1 (en) * 2002-03-29 2003-10-16 Merck & Co., Inc. Methods of virus production
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
WO2006014839A2 (en) * 2004-07-23 2006-02-09 The University Of Rochester Activated protein c inhibits undesirable effects of plasminogen activator in the brain
EP1913155A4 (en) * 2005-06-23 2009-08-12 Univ British Columbia COAGULATION FACTOR III POLYMORPHISMS ASSOCIATED WITH PREDICTION RELATING TO THE RESULTS AND RESPONSE OF A SUBJECT TO THERAPY
EP2041338A4 (en) * 2006-06-09 2009-09-30 Univ British Columbia INTERFERON GAMMA POLYMORPHISMS AS INDICATORS OF PATIENT RESULTS IN FATIGUE PATIENTS
US20110171200A1 (en) * 2008-01-15 2011-07-14 Walley Keith R Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
EP0289586A4 (en) * 1986-11-17 1990-04-10 New England Medical Ct INCREASING GAMMA CARBOXYLATION OF RECOMBINANT, VITAMIN K-DEPENDENT PROTEINS.
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
CA2064774C (en) * 1989-08-11 2000-01-11 Anur A. Kumar Culture methods for producing activated protein c
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
JPH05276952A (ja) * 1991-01-29 1993-10-26 Teijin Ltd ヒトプロテインｃ発現ベクター

Also Published As

Publication number Publication date
US5618714A (en) 1997-04-08
DE69425809T2 (de) 2001-02-01
RU2110574C1 (ru) 1998-05-10
IL111983A0 (en) 1995-03-15
FI106385B (fi) 2001-01-31
EP0658626B1 (en) 2000-09-06
EP0658626A1 (en) 1995-06-21
HUT69979A (en) 1995-09-28
HU9403600D0 (en) 1995-02-28
FI945877A (fi) 1995-06-16
BR9404991A (pt) 1995-08-08
JP3662614B2 (ja) 2005-06-22
CO4340654A1 (es) 1996-07-30
KR950018454A (ko) 1995-07-22
CA2138101A1 (en) 1995-06-16
PL176381B1 (pl) 1999-05-31
HU219508B (hu) 2001-04-28
NO944846D0 (no) 1994-12-14
SI0658626T1 (en) 2001-02-28
NO944846L (no) 1995-06-16
CZ315794A3 (en) 1995-10-18
PL306291A1 (en) 1995-06-26
AU8045294A (en) 1995-06-22
NZ270140A (en) 1996-05-28
RU94043769A (ru) 1997-03-27
JPH07222589A (ja) 1995-08-22
ES2149244T3 (es) 2000-11-01
PT658626E (pt) 2001-01-31
FI945877A0 (fi) 1994-12-14
PE42695A1 (es) 1995-12-12
ZA949981B (en) 1996-06-14
DK0658626T3 (da) 2000-10-16
GR3034909T3 (en) 2001-02-28
AU687914B2 (en) 1998-03-05
CN1107887A (zh) 1995-09-06
CZ290724B6 (cs) 2002-10-16
ATE196165T1 (de) 2000-09-15
YU73494A (sh) 1997-03-07
DE69425809D1 (de) 2000-10-12

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