PE42695A1 - Procedimiento para producir proteina c - Google Patents

Procedimiento para producir proteina c

Info

Publication number
PE42695A1
PE42695A1 PE1994257065A PE25706594A PE42695A1 PE 42695 A1 PE42695 A1 PE 42695A1 PE 1994257065 A PE1994257065 A PE 1994257065A PE 25706594 A PE25706594 A PE 25706594A PE 42695 A1 PE42695 A1 PE 42695A1
Authority
PE
Peru
Prior art keywords
procedure
cell lines
degrees centigrade
recombinant proteins
produce protein
Prior art date
Application number
PE1994257065A
Other languages
English (en)
Inventor
Brian William Grinnell
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of PE42695A1 publication Critical patent/PE42695A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE REFIERE A UN PROCEDIMIENTO PARA INCREMENTAR LA PRODUCCION DE LA PROTEINA C QUE TIENE ACTIVIDAD ANTICOAGULANTE O PROFIBRINOLITICA; EN UNA CELULA HUESPED MAMIFERA RECOMBINANTE TRANSFORMADA CON ADENOVIRUS TAL COMO: 1) LA LINEA CELULAR HUMAN KIDNEY 293; 2) LA LINEA DE CELULAS SYRIAM HAMSTER AV12; CARACTERIZADO POR COMPRENDER EL CULTIVO DE DICHAS CELULAS A UNA TEMPERATURA DE APROXIMADAMENTE 38°C A 39°C, QUE INCREMENTA EN EL CASO DE (1) LA PRODUCCION DE LA SUSTANCIA C Y EN EL CASO (2) LA PRODUCCION DE MODIFICACIONES POST-TRADUCCION TAL COMO GAMMA-CARBOXILACION DURANTE LA MADURACION NECESARIA PARA LA PRODUCCION DE PROTEINA C ACTIVA
PE1994257065A 1993-12-15 1994-12-14 Procedimiento para producir proteina c PE42695A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (1)

Publication Number Publication Date
PE42695A1 true PE42695A1 (es) 1995-12-12

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1994257065A PE42695A1 (es) 1993-12-15 1994-12-14 Procedimiento para producir proteina c

Country Status (28)

Country Link
US (1) US5618714A (es)
EP (1) EP0658626B1 (es)
JP (1) JP3662614B2 (es)
KR (1) KR950018454A (es)
CN (1) CN1107887A (es)
AT (1) ATE196165T1 (es)
AU (1) AU687914B2 (es)
BR (1) BR9404991A (es)
CA (1) CA2138101A1 (es)
CO (1) CO4340654A1 (es)
CZ (2) CZ315794A3 (es)
DE (1) DE69425809T2 (es)
DK (1) DK0658626T3 (es)
ES (1) ES2149244T3 (es)
FI (1) FI106385B (es)
GR (1) GR3034909T3 (es)
HU (1) HU219508B (es)
IL (1) IL111983A0 (es)
NO (1) NO944846L (es)
NZ (1) NZ270140A (es)
PE (1) PE42695A1 (es)
PL (1) PL176381B1 (es)
PT (1) PT658626E (es)
RU (1) RU2110574C1 (es)
SI (1) SI0658626T1 (es)
UA (1) UA26870C2 (es)
YU (1) YU73494A (es)
ZA (1) ZA949981B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
WO2002069232A2 (en) 2001-02-19 2002-09-06 Merck Patent Gmbh Method for identification of t-cell epitopes and use for preparing molecules with reeduced immunogenicity
AU2003226012B2 (en) 2002-03-29 2007-05-24 Merck Sharp & Dohme Corp. Methods of virus production
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
EP1773371A4 (en) * 2004-07-23 2009-12-30 Univ Rochester ACTIVATED PROTEIN C INHIBITS SIDE EFFECTS OF PLASMINOGEN ACTIVATOR IN THE BRAIN
WO2006136033A1 (en) * 2005-06-23 2006-12-28 The University Of British Columbia Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy
WO2007140625A1 (en) * 2006-06-09 2007-12-13 The University Of British Columbia Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects
WO2009089620A1 (en) * 2008-01-15 2009-07-23 The Universityof British Columbia Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
EP0289586A4 (en) * 1986-11-17 1990-04-10 New England Medical Ct INCREASING GAMMA CARBOXYLATION OF RECOMBINANT, VITAMIN K-DEPENDENT PROTEINS.
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
ES2047946T3 (es) * 1989-08-11 1994-03-01 Zymogenetics Inc Metodos de cultivos celulares para producir proteina c activada.
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
AU1180692A (en) * 1991-01-29 1992-08-27 Teijin Limited Human protein c expression vector

Also Published As

Publication number Publication date
FI945877A (fi) 1995-06-16
FI106385B (fi) 2001-01-31
AU8045294A (en) 1995-06-22
UA26870C2 (uk) 1999-12-29
HU219508B (hu) 2001-04-28
FI945877A0 (fi) 1994-12-14
CZ290724B6 (cs) 2002-10-16
HU9403600D0 (en) 1995-02-28
CZ315794A3 (en) 1995-10-18
HUT69979A (en) 1995-09-28
EP0658626A1 (en) 1995-06-21
IL111983A0 (en) 1995-03-15
DE69425809D1 (de) 2000-10-12
EP0658626B1 (en) 2000-09-06
JPH07222589A (ja) 1995-08-22
RU2110574C1 (ru) 1998-05-10
AU687914B2 (en) 1998-03-05
DE69425809T2 (de) 2001-02-01
KR950018454A (ko) 1995-07-22
PL306291A1 (en) 1995-06-26
YU73494A (sh) 1997-03-07
CA2138101A1 (en) 1995-06-16
NZ270140A (en) 1996-05-28
BR9404991A (pt) 1995-08-08
GR3034909T3 (en) 2001-02-28
US5618714A (en) 1997-04-08
NO944846L (no) 1995-06-16
DK0658626T3 (da) 2000-10-16
ATE196165T1 (de) 2000-09-15
JP3662614B2 (ja) 2005-06-22
CN1107887A (zh) 1995-09-06
NO944846D0 (no) 1994-12-14
ES2149244T3 (es) 2000-11-01
PL176381B1 (pl) 1999-05-31
SI0658626T1 (en) 2001-02-28
PT658626E (pt) 2001-01-31
RU94043769A (ru) 1997-03-27
ZA949981B (en) 1996-06-14
CO4340654A1 (es) 1996-07-30

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Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed