DE69425809T2 - Methoden zur Herstellung von Protein C - Google Patents

Methoden zur Herstellung von Protein C

Info

Publication number
DE69425809T2
DE69425809T2 DE69425809T DE69425809T DE69425809T2 DE 69425809 T2 DE69425809 T2 DE 69425809T2 DE 69425809 T DE69425809 T DE 69425809T DE 69425809 T DE69425809 T DE 69425809T DE 69425809 T2 DE69425809 T2 DE 69425809T2
Authority
DE
Germany
Prior art keywords
methods
cell lines
degrees centigrade
recombinant proteins
making protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69425809T
Other languages
English (en)
Other versions
DE69425809D1 (de
Inventor
Brian William Grinnell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cardiome Pharma Corp
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of DE69425809D1 publication Critical patent/DE69425809D1/de
Application granted granted Critical
Publication of DE69425809T2 publication Critical patent/DE69425809T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69425809T 1993-12-15 1994-12-14 Methoden zur Herstellung von Protein C Expired - Lifetime DE69425809T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (2)

Publication Number Publication Date
DE69425809D1 DE69425809D1 (de) 2000-10-12
DE69425809T2 true DE69425809T2 (de) 2001-02-01

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69425809T Expired - Lifetime DE69425809T2 (de) 1993-12-15 1994-12-14 Methoden zur Herstellung von Protein C

Country Status (28)

Country Link
US (1) US5618714A (de)
EP (1) EP0658626B1 (de)
JP (1) JP3662614B2 (de)
KR (1) KR950018454A (de)
CN (1) CN1107887A (de)
AT (1) ATE196165T1 (de)
AU (1) AU687914B2 (de)
BR (1) BR9404991A (de)
CA (1) CA2138101A1 (de)
CO (1) CO4340654A1 (de)
CZ (2) CZ315794A3 (de)
DE (1) DE69425809T2 (de)
DK (1) DK0658626T3 (de)
ES (1) ES2149244T3 (de)
FI (1) FI106385B (de)
GR (1) GR3034909T3 (de)
HU (1) HU219508B (de)
IL (1) IL111983A0 (de)
NO (1) NO944846L (de)
NZ (1) NZ270140A (de)
PE (1) PE42695A1 (de)
PL (1) PL176381B1 (de)
PT (1) PT658626E (de)
RU (1) RU2110574C1 (de)
SI (1) SI0658626T1 (de)
UA (1) UA26870C2 (de)
YU (1) YU73494A (de)
ZA (1) ZA949981B (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
CA2438652A1 (en) 2001-02-19 2002-09-06 Merck Patent Gesellschaft Mit Beschraenkter Haftung Method for identification of t-cell epitopes and use for preparing molecules with reeduced immunogenicity
ATE386824T1 (de) 2002-03-29 2008-03-15 Merck & Co Inc Verfahren zur virusproduktion
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
US20080305100A1 (en) * 2004-07-23 2008-12-11 Zlokovic Berislav V Activated Protein C Inhibits Undesirable Effects of Plasminogen Activator in the Brain
EP1913155A4 (de) * 2005-06-23 2009-08-12 Univ British Columbia Polymorphismen in gerinnungsfaktor iii im zusammenhang mit der vorhersage des ergebnisses für den patienten und des ansprechens des patienten auf eine therapie
CA2654761A1 (en) * 2006-06-09 2007-12-13 The University Of British Columbia Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects
WO2009089620A1 (en) * 2008-01-15 2009-07-23 The Universityof British Columbia Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
EP0245949B1 (de) * 1986-04-09 1997-10-29 Eli Lilly And Company Verfahren zur Anwendung von den BK-Virus-Verstärker enthaltenden eukaryotischen Expressionsvektoren
EP0289586A4 (de) * 1986-11-17 1990-04-10 New England Medical Ct Steigerung der gamma-carboxylation von rekombinanten, vitamin-k-abhängigen proteinen.
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
JP3045307B2 (ja) * 1989-08-11 2000-05-29 ザイモジェネティクス，インコーポレイティド 活性化プロテインｃを生成するための細胞培養法
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
AU1180692A (en) * 1991-01-29 1992-08-27 Teijin Limited Human protein c expression vector

Also Published As

Publication number Publication date
PT658626E (pt) 2001-01-31
ATE196165T1 (de) 2000-09-15
FI106385B (fi) 2001-01-31
NO944846L (no) 1995-06-16
CO4340654A1 (es) 1996-07-30
BR9404991A (pt) 1995-08-08
AU687914B2 (en) 1998-03-05
JPH07222589A (ja) 1995-08-22
CZ315794A3 (en) 1995-10-18
FI945877A0 (fi) 1994-12-14
PL306291A1 (en) 1995-06-26
NZ270140A (en) 1996-05-28
FI945877A (fi) 1995-06-16
PE42695A1 (es) 1995-12-12
RU2110574C1 (ru) 1998-05-10
HU9403600D0 (en) 1995-02-28
RU94043769A (ru) 1997-03-27
UA26870C2 (uk) 1999-12-29
ZA949981B (en) 1996-06-14
CZ290724B6 (cs) 2002-10-16
EP0658626A1 (de) 1995-06-21
GR3034909T3 (en) 2001-02-28
DE69425809D1 (de) 2000-10-12
IL111983A0 (en) 1995-03-15
ES2149244T3 (es) 2000-11-01
AU8045294A (en) 1995-06-22
HU219508B (hu) 2001-04-28
YU73494A (sh) 1997-03-07
JP3662614B2 (ja) 2005-06-22
DK0658626T3 (da) 2000-10-16
CN1107887A (zh) 1995-09-06
HUT69979A (en) 1995-09-28
SI0658626T1 (en) 2001-02-28
KR950018454A (ko) 1995-07-22
US5618714A (en) 1997-04-08
PL176381B1 (pl) 1999-05-31
EP0658626B1 (de) 2000-09-06
CA2138101A1 (en) 1995-06-16
NO944846D0 (no) 1994-12-14

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: CARDIOME PHARMA CORP., VANCOUVER, CA

8328 Change in the person/name/address of the agent

Representative=s name: KROHER, STROBEL RECHTS- UND PATENTANWAELTE, 80336

8328 Change in the person/name/address of the agent

Representative=s name: DR. SCHOEN & PARTNER, 80336 MUENCHEN